The Crystal Structure Of Sulfolobus Solfataricus Elongation Factor 1 Alpha In Complex With Magnesium And Gdp(478 views) Vitagliano L, Ruggiero A, Masullo M, Cantiello P, Arcari P, Zagari A
Ist. di Biostrutture e Bioimmagini, CNR, via Mezzocannone 6, I-80134 Napoli, Italy
Ctro. Interuniv. Ric. Peptidi B., via Mezzocannone 6, Roccelletta Borgia I-80134 Napoli, Italy
Dipartimento di Chimica Biologica, Sezione di Biostrutture, Univ. degli Studi Napoli Federico II, via Mezzocannone 6, I-80134 Napoli, Italy
Dipto. di Scienze Farmacobiologiche, Univ. Studi Catanzaro Magna Graecia, 88021 Catanzaro, Italy
Dipto. di Biochim. e Biotecnol. Med., Univ. Studi di Napoli Federico II, Via Pansini 5, I-80131 Napoli, Italy
CEINGE, Biotecnologie Avanzate Scarl, Via Comunale Margherita 482, I-80145 Napoli, Italy
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Andersen, G. R., Nissen, P., Nyborg, J., Elongation factors in protein biosynthesis (2003) Trends Biochem. Sci., 28, pp. 434-441
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Heffron, S. E., Jurnak, F., Structure of an EF-Tu complex with a thiazolyl peptide antibiotic determined at 2. 35 resolution: Atomic basis for GE2270A inhibition of EF-Tu (2000) Biochemistry, 39, pp. 37-45
Andersen, G. R., Pedersen, L., Valente, L., Chatterjee, I., Kinzy, T. G., Kjeldgaard, M., Nyborg, J., Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A: eEF1Balpha (2000) Mol. Cell, 6, pp. 1261-1266
Andersen, G. R., Valente, L., Pedersen, L., Kinzy, T. G., Nyborg, J., Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex (2001) Nat. Struct. Biol., 8, pp. 531-534
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Kraulis, P. J., MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures (1991) J. Appl. Crystallogr., 24, pp. 946-950
Esnouf, R. M., Further additions to MolScript version 1. 4, including reading and conturing of electron-density maps (1999) Acta Crystallogr., Sect. D: Biol. Crystallogr., 55, pp. 938-940
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The Crystal Structure Of Sulfolobus Solfataricus Elongation Factor 1 Alpha In Complex With Magnesium And Gdp
Recent studies have shown that elongation factors extracted from archaea/eukarya and from eubacteria exhibit different structural and functional properties. Along this line, it has been demonstrated that, in contrast to EF-Tu, Sulfolobus solfataricus EF-1alpha in complex with GDP (SsEF-1alpha. GDP) does not bind Mg2+, when the ion is present in the crystallization medium at moderate concentration (5 mM). To further investigate the role that magnesium plays in the exchange process of EF-lalpha and to check the ability of SsEF-1alpha. GDP to bind the ion, we have determined the crystal structure of SsEF-1alpha. GDP in the presence of a nonphysiological concentration (100 mM) of Mg2+. The analysis of the coordination of Mg2+ unveils the structural bases for the marginal role played by the ion in the nucleotide exchange process. Furthermore, nucleotide exchange experiments carried out on a truncated form of SsEF-1alpha, consisting only of the nucleotide binding domain, demonstrate that the low affinity of SsEF-1alpha. GDP for Mg2+ is due to the local architecture of the active site and does not depend on the presence of the other two domains. Finally, considering the available structures of EF-1alpha, a detailed mechanism for the nucleotide exchange process has been traced. Notably, this mechanism involves residues such as His14, Arg95, Gln131, and Glu134, which are strictly conserved in all archaea and eukarya EF-1alpha sequences hitherto reported
The Crystal Structure Of Sulfolobus Solfataricus Elongation Factor 1 Alpha In Complex With Magnesium And Gdp
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(297 views) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 ViewExport to BibTeXExport to EndNote