Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production(476 views) Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S
Keywords: Beta Interferon, Cell Surface Protein, Immunoglobulin Enhancer Binding Protein, Interleukin 10, Mitogen Activated Protein Kinase, Virus Fusion Protein, Article, Cell Membrane, Controlled Study, Cytokine Production, Enzyme Linked Immunosorbent Assay, Filoviridae, Flavivirus, Human, Human Cell, Immunoprecipitation, In Vitro Study, Nonhuman, Orthomyxovirus, Paramyxovirus, Priority Journal, Protein Domain, Protein Interaction, Protein Phosphorylation, Protein Synthesis, Retrovirus, Rhabdovirus, Signal Transduction, Solid Phase Synthesis, Src Homology Domain, Strain Difference, Togavirus, Virus Entry, Virus Strain, Western Blotting, Cell Line, Enzyme-Linked Immunosorbent Assay, Host-Pathogen Interactions, Interferon-Beta, Interleukin-10, Monocytes, Transcriptional Activation, Viral Fusion Proteins,
Affiliations: *** IBB - CNR ***
Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy.
Department of Biological Sciences, University of Naples 'Federico II', Institute of Biostructures and Bioimaging, Naples, Italy
References: Ahmad, R., Ennaciri, J., Cordeiro, P., El Bassam, S., Menezes, J., Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways (2007) J. Mol. Biol., 367, pp. 25-3
Azran-Shaish, I., Tabakin-Fix, Y., Huleihel, M., Bakhanashvili, M., Aboud, M., HTLV-1 tax-induced NF-kappaB activation is synergistically enhanced by 12-O-tetradecanoylphorbol-13-acetate: Mechanism and implications for Tax oncogenicity (2008) J. Mol. Med., 86, pp. 799-814
Basbous, J., Arpin, C., Gaudray, G., Devaux, C.P., Mesnard, J.M., The HBZ factor of human T-cell leukaemia virus type I dimerizes with transcription factors JunB and c-Jun. and modulates their transcriptional activity (2003) J. Biol. Chem., 278, pp. 43620-43627
Cooray, S., The pivotal role of phosphatidylinositol 3-kinase-Akt signal transduction in virus survival (2004) J. Gen. Virol., 85, pp. 1065-1076
Falvo, J.V., Parekh, B.S., Lin, C.H., Fraenkel, E., Maniatis, T., Assembly of a functional beta interferon enhanceosome is dependent on ATF-2-c-jun heterodimer orientation (2000) Mol. Cell. Biol., 20, pp. 4814-4825
Galdiero, M., Tortora, A., Damiano, N., Vitiello, M., Longanella, A., Galdiero, E., Induction of cytokine mRNA expression in U937 cells by Salmonella typhimurium porins is regulated by different phosphorylation pathways (2005) Med. Microbiol. Immunol., 194, pp. 13-23
He, R., Leeson, A., Andonov, A., Li, Y., Bastien, N., Cao, J., Osiowy, C., Li, X., Activation of AP-1 signal transduction pathway by SARS coronavirus nucleocapsid protein (2003) Biochem. Biophys. Res. Commun., 311, pp. 870-876
Heldwein, E.E., Lou, H., Bender, F.C., Cohen, G.H., Eisenberg, R.J., Harrison, S.C., Crystal structure of glycoprotein B from herpes simplex virus 1 (2006) Science, 313, pp. 217-220
Hess, J., Angel, P., Schorpp-Kistner, M., AP-1 subunits: Quarrel and harmony among siblings (2004) J. Cell. Sci., 117, pp. 5965-5973
Hiscott, J., Convergence of the NF-kappaB and IRF pathways in the regulation of the innate antiviral response (2007) Cytokine Growth Factor Rev., 18, pp. 483-490
Huang, C.F., Lin, S.S., Ho, Y.C., Chen, F.L., Yang, C.C., The immune response induced by hepatitis B virus principal antigens (2006) Cell. Mol. Immunol., 3, pp. 97-106
Irshad, M., Khushboo, I., Singh, S., Singh, S., Hepatitis C virus (HCV): A review of immunological aspects (2008) Int. Rev. Immunol., 27, pp. 497-517
Kim, S., Yu, S.S., Lee, I.S., Ohno, S., Yim, J., Kim, S., Kang, H.S., Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase (s) (1999) J. Gen. Virol., 80, pp. 961-969
Kim, S.Y., Kim, J.C., Kim, J.K., Kim, H.J., Lee, H.M., Choi, M.S., Maeng, P.J., Ahn, J.K., Hepatitis B virus X protein enhances NFkappaB activity through cooperating with VBP1 (2008) BMB Rep., 41, pp. 158-163
Kong, X., Juan, H.S., Behera, A., Peeples, M.E., Wu, J., Lockey, R.F., Mohapatra, S.S., ERK-1/2 activity is required for efficient RSV infection (2004) FEBS Lett., 559, pp. 33-38
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
Mettling, C., Desmetz, C., Fiser, A.L., Réant, B., Corbeau, P., Lin, Y.L., Galphai protein-dependant extracellular signal-regulated kinase-1/2 activation is required for HIV-1 reverse transcription (2008) AIDS, 22, pp. 1569-1576
Monick, M., Staber, J., Thomas, K., Hunninghake, G., Respiratory syncytial virus infection results in activation of multiple protein kinase C isoforms leading to activation of mitogen-activated protein kinase (2001) J. Immunol., 166, pp. 2681-2687
Monje, P., Marinissen, M.J., Gutkind, J.S., Phosphorylation of the carboxyl-terminal transactivation domain of c-Fos by extracellular signal-regulated kinase mediates the transcriptional activation of AP-1 and cellular transformation induced by platelet-derived growth factor (2003) Mol. Cell. Biol., 23, pp. 7030-7043
Murphy, L.O., Smith, S., Chen, R.H., Fingar, D.C., Blenis, J., Molecular interpretation of ERK signal duration by immediate early gene products (2002) Nat. Cell. Biol., 4, pp. 556-564
O'Garra, A., Barrat, F.J., Castro, A.G., Vicari, A., Hawrylowicz, C., Strategies for use of IL-10 or its antagonists in human disease (2008) Immunol. Rev. Jun, 223, pp. 114-131
Pahl, H.L., Sester, M., Burgert, H.G., Baeuerle, P.A., Activation of transcription factor NF-kappaB by the adenovirus E3/19K protein requires its ER retention (1996) J. Cel L Biol., 132, pp. 511-522
Pleschka, S., RNA viruses and the mitogenic Raf/MEK/ERK signal transduction cascade (2008) Biol. Chem., 389, pp. 1273-1282
Renard, P., Ernest, I., Houbion, A., Art, M., Le Calvez, H., Raes, M., Remacle, J., Development of a sensitive multi-well colorimetric assay for active NFkB (2001) Nucl. Acids Res., 29, pp. E21
Roche, S., Bressanelli, S., Rey, F.A., Gaudin, Y., Crystal structure of the low-pH form of the vesicular stomatitis virus glycoprotein G (2006) Science, 313, pp. 187-191
Roche, S., Rey, F.A., Gaudin, Y., Bressanelli, S., Structure of the perfusion form of the vesicular stomatitis virus glycoprotein G (2007) Science, 315, pp. 843-848
Sieczkarski, S.B., Brown, H.A., Whittaker, G.R., Role of protein kinase C betaII in influenza virus entry via late endosomes (2003) J. Virol., 77, pp. 460-469
Smith, C.C., The herpes simplex virus type 2 protein ICP10PK: A master of versatility (2005) Front Biosci., 10, pp. 2820-2831
Sumbayev, V.V., Yasinska, I.M., Role of MAP kinase-dependent apoptotic pathway in innate immune responses and viral infection (2006) Scand. J. Immunol., 63, pp. 391-400
Sun, M., Fuentes, S.M., Timani, K., Sun, D., Murphy, C., Lin, Y., August, A., He, B., Akt plays a critical role in replication of nonsegmented negative-stranded RNA viruses (2008) J. Virol., 82, pp. 105-114
Tanaka, Y., Kanai, F., Ichimura, T., Tateishi, K., Asaoka, Y., Leng, B.G., Zag, A.J., Omata, M., The hepatitis B virus X protein enhances AP-1 activation through interaction with Jab1 (2006) Oncogene, 25, pp. 633-642
Unterstab, G., Ludwig, S., Anton, A., Planz, O., Dauber, B., Krappmann, D., Heins, G., Wolff, T., Viral targeting of the interferon-{beta}-inducing Traf family member-associated NF-{kappa}B activator (TANK)-binding kinase-1 (2005) Proc. Natl. Acad. Sci. USA, 102, pp. 13640-13645
White, J.M., Delos, S.E., Brecher, M., Schornberg, K., Structures and mechanisms of viral membrane fusion proteins: Multiple variations on a common theme (2008) Crit. Rev. Biochem. Mol. Biol., 43, pp. 189-219
Yin, E.T., Galanos, C., Kinsky, S., Bradshaw, R.A., Wessler, S., Lüderitz, O., Sarmiento, M.E., Picogram-sensitive assay for endotoxin: Gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from gram-negative bacteria (1972) Biochim. Biophys. Acta, 261, pp. 284-289
Zheng, H., Li, L.L., Hu, D.S., Deng, X.Y., Cao, Y., Role of Epstein-Barr virus encoded latent membrane protein 1 in the carcinogenesis of nasopharyngeal carcinoma (2007) Cell. Mol. Immunol., 4, pp. 185-196
Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
OBJECTIVES: The deciphering of intracellular signaling pathways that are activated by the interaction between viral fusion peptides and cellular membranes are important for the understanding of both viral replication strategies and host defense mechanisms.; METHODS: Fusion peptides of several enveloped viruses belonging to different virus families were prepared by standard 9-fluorenylmethoxycarbonyl polyamine solid-phase synthesis and used to stimulate U937 cells in vitro to analyze the phosphorylation patterns of the signaling pathways (PKC, Src, Akt, and MAPK pathways). Immunoprecipitation and Western blotting were carried out by using phosphospecific antibodies. All samples were also assayed for the presence of IL-10 and IFN-beta by ELISA and activation of nuclear factors (AP-1 and NF-kappaB).; RESULTS: We have demonstrated that hydrophobic domains of fusion proteins are able to induce several transduction pathways that lead to cytokine (IFN-beta and IL-10) production, an event that appears to be dependent on early activation of AP-1 and NF-kappaB.; CONCLUSIONS: The results obtained on the signaling activity of fusion peptides from different viruses enabled us to shed some light on the complex mechanism of viral entry and more precisely we focused on the exact signaling event induced by hydrophobic domains characteristic of fusion peptides interacting with the cell membrane. Copyright 2010 S. Karger AG, Basel.
Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
No results.
Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(479 views) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 ViewExport to BibTeXExport to EndNote