Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production(684 views) Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S
Keywords: Beta Interferon, Cell Surface Protein, Immunoglobulin Enhancer Binding Protein, Interleukin 10, Mitogen Activated Protein Kinase, Virus Fusion Protein, Article, Cell Membrane, Controlled Study, Cytokine Production, Enzyme Linked Immunosorbent Assay, Filoviridae, Flavivirus, Human, Human Cell, Immunoprecipitation, In Vitro Study, Nonhuman, Orthomyxovirus, Paramyxovirus, Priority Journal, Protein Domain, Protein Interaction, Protein Phosphorylation, Protein Synthesis, Retrovirus, Rhabdovirus, Signal Transduction, Solid Phase Synthesis, Src Homology Domain, Strain Difference, Togavirus, Virus Entry, Virus Strain, Western Blotting, Cell Line, Enzyme-Linked Immunosorbent Assay, Host-Pathogen Interactions, Interferon-Beta, Interleukin-10, Monocytes, Transcriptional Activation, Viral Fusion Proteins,
Affiliations: *** IBB - CNR ***
Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy.
Department of Biological Sciences, University of Naples 'Federico II', Institute of Biostructures and Bioimaging, Naples, Italy
References: Not available.
Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
OBJECTIVES: The deciphering of intracellular signaling pathways that are activated by the interaction between viral fusion peptides and cellular membranes are important for the understanding of both viral replication strategies and host defense mechanisms.; METHODS: Fusion peptides of several enveloped viruses belonging to different virus families were prepared by standard 9-fluorenylmethoxycarbonyl polyamine solid-phase synthesis and used to stimulate U937 cells in vitro to analyze the phosphorylation patterns of the signaling pathways (PKC, Src, Akt, and MAPK pathways). Immunoprecipitation and Western blotting were carried out by using phosphospecific antibodies. All samples were also assayed for the presence of IL-10 and IFN-beta by ELISA and activation of nuclear factors (AP-1 and NF-kappaB).; RESULTS: We have demonstrated that hydrophobic domains of fusion proteins are able to induce several transduction pathways that lead to cytokine (IFN-beta and IL-10) production, an event that appears to be dependent on early activation of AP-1 and NF-kappaB.; CONCLUSIONS: The results obtained on the signaling activity of fusion peptides from different viruses enabled us to shed some light on the complex mechanism of viral entry and more precisely we focused on the exact signaling event induced by hydrophobic domains characteristic of fusion peptides interacting with the cell membrane. Copyright 2010 S. Karger AG, Basel.
Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
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Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(680 views) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 ViewExport to BibTeXExport to EndNote