Generation And Functional Characterization Of A Bcl10-Inhibitory Peptide That Represses Nf-Kappa B Activation (895 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2009 Sep 15; 422(3): 553-561.
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Paper type: Journal Article,
Impact factor: 5.155, 5-year impact factor: 4.365
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-70249094317&partnerID=40&md5=87926be1bec7103dcecce36cbc1e7a85
Keywords: Bcl10-Inhibitory Peptide, Caspase Recruitment Domain (card), Caspase Recruitment Domain-Containing Membrane-Associated Guanylate Kinase (carma), Nuclear Factor κb (nf-κb), Amino Acid Residues, Functional Characterization, In-Cell, Key Component, Lymphoid Cells, Molecular Complexes, Pathological Conditions, Self-Associations, Signal Transduction Pathways, Cell Membranes, Employment, Organic Acids, Transcription Factors, Association Reactions, Caspase Recruitment Domain Signaling Protein, Immunoglobulin Enhancer Binding Protein, Membrane Associated Guanylate Cyclase Kinase, Protein Bcl 10, Bcl10 Protein, Human, Signal Transducing Adaptor Protein, Amino Acid Sequence, Amino Terminal Sequence, Article, Controlled Study, Enzyme Activation, Enzyme Repression, Human Cell, Liquid Chromatography, Priority Journal, Protein Assembly, Protein Domain, Protein Expression, Protein Motif, Protein Protein Interaction, Tandem Mass Spectrometry, Cell Line, Chemistry, Drug Antagonism, Enzyme Linked Immunosorbent Assay, Genetics, Immunoblotting, Immunoprecipitation, Metabolism, Molecular Genetics, Physiology, Protein Binding, Protein Tertiary Structure, Card Signaling Adaptor Proteins, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Nf-Kappa B, Protein Structure, Nuclear Factor B (nf-B),
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini (IBB), CNR, via Mezzocannone 16, 80134 Napoli, Italy
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 16, 80134 Napoli, Italy
BioGeM Consortium, Via Camporeale, 83031 Ariano Irpino, Italy
Centro di Endocrinologia ed Oncologia Sperimentale, 80131 Napoli, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy
References:
Not available.
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2009 Sep 15; 422(3): 553-561.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 5.155, 5-year impact factor: 4.365
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-70249094317&partnerID=40&md5=87926be1bec7103dcecce36cbc1e7a85
Keywords: Bcl10-Inhibitory Peptide, Caspase Recruitment Domain (card), Caspase Recruitment Domain-Containing Membrane-Associated Guanylate Kinase (carma), Nuclear Factor κb (nf-κb), Amino Acid Residues, Functional Characterization, In-Cell, Key Component, Lymphoid Cells, Molecular Complexes, Pathological Conditions, Self-Associations, Signal Transduction Pathways, Cell Membranes, Employment, Organic Acids, Transcription Factors, Association Reactions, Caspase Recruitment Domain Signaling Protein, Immunoglobulin Enhancer Binding Protein, Membrane Associated Guanylate Cyclase Kinase, Protein Bcl 10, Bcl10 Protein, Human, Signal Transducing Adaptor Protein, Amino Acid Sequence, Amino Terminal Sequence, Article, Controlled Study, Enzyme Activation, Enzyme Repression, Human Cell, Liquid Chromatography, Priority Journal, Protein Assembly, Protein Domain, Protein Expression, Protein Motif, Protein Protein Interaction, Tandem Mass Spectrometry, Cell Line, Chemistry, Drug Antagonism, Enzyme Linked Immunosorbent Assay, Genetics, Immunoblotting, Immunoprecipitation, Metabolism, Molecular Genetics, Physiology, Protein Binding, Protein Tertiary Structure, Card Signaling Adaptor Proteins, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Nf-Kappa B, Protein Structure, Nuclear Factor B (nf-B),
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini (IBB), CNR, via Mezzocannone 16, 80134 Napoli, Italy
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 16, 80134 Napoli, Italy
BioGeM Consortium, Via Camporeale, 83031 Ariano Irpino, Italy
Centro di Endocrinologia ed Oncologia Sperimentale, 80131 Napoli, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy
References:
Not available.
Generation And Functional Characterization Of A Bcl10-Inhibitory Peptide That Represses Nf-Kappa B Activation
The molecular complex containing BCL10 and CARMA [CARD (caspase recruitment domain)-containing MAGUK (membrane-associated guanylate kinase)] proteins has recently been identified as a key component in the signal transduction pathways that regulate activation of the transcription factor NF-κB (nuclear factor κB) in lymphoid and non-lymphoid cells. Assembly of complexes containing BCL10 and CARMA proteins relies on homophilic interactions established between the CARDs of these proteins. In order to identify BCL10-inhibitory peptides, we have established a method of assaying peptides derived from the CARD of BCL10 in binding competition assays of CARD-CARD self-association. By this procedure, a short peptide corresponding to amino acid residues 91-98 of BCL10 has been selected as an effective inhibitor of protein self-association. When tested in cell assays for its capacity to block NF-κB activation, this peptide represses activation of NF-κB mediated by BCL10, CARMA3 and PMA/ionomycin stimulation. Collectively, these results indicate that residues 91-98 of BCL10 are involved in BCL10 self-association and also participate in the interaction with external partners. We also show that blocking of the CARD of BCL10 may potentially be used for the treatment of pathological conditions associated with inappropriate NF-ΚB activation. © The Authors Journal compilation. © 2009 Biochemical Society.
The molecular complex containing BCL10 and CARMA [CARD (caspase recruitment domain)-containing MAGUK (membrane-associated guanylate kinase)] proteins has recently been identified as a key component in the signal transduction pathways that regulate activation of the transcription factor NF-κB (nuclear factor κB) in lymphoid and non-lymphoid cells. Assembly of complexes containing BCL10 and CARMA proteins relies on homophilic interactions established between the CARDs of these proteins. In order to identify BCL10-inhibitory peptides, we have established a method of assaying peptides derived from the CARD of BCL10 in binding competition assays of CARD-CARD self-association. By this procedure, a short peptide corresponding to amino acid residues 91-98 of BCL10 has been selected as an effective inhibitor of protein self-association. When tested in cell assays for its capacity to block NF-κB activation, this peptide represses activation of NF-κB mediated by BCL10, CARMA3 and PMA/ionomycin stimulation. Collectively, these results indicate that residues 91-98 of BCL10 are involved in BCL10 self-association and also participate in the interaction with external partners. We also show that blocking of the CARD of BCL10 may potentially be used for the treatment of pathological conditions associated with inappropriate NF-ΚB activation. © The Authors Journal compilation. © 2009 Biochemical Society.
Generation And Functional Characterization Of A Bcl10-Inhibitory Peptide That Represses Nf-Kappa B Activation
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Generation And Functional Characterization Of A Bcl10-Inhibitory Peptide That Represses Nf-Kappa B Activation
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Alterio V, Monti SM, Truppo E, Pedone C, Supuran CT, De Simone G
* The first example of a significant active site conformational rearrangement in a carbonic anhydrase-inhibitor adduct: The carbonic anhydrase I-topiramate complex (1754 views)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2010 Sep 7; 8(15): 3528-3533.
Impact Factor: 3.451
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La Mendola D, Magrì A, Campagna T, Campitiello MA, Raiola L, Isernia C, Hansson O, Bonomo RP, Rizzarelli E
* A doppel alpha-helix peptide fragment mimics the copper(II) interactions with the whole protein (1762 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2010 Jun 1; 16(21): 6212-6223.
Impact Factor: 5.476
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Zubrzak P, Banas A, Kaczmarek K, Leplawy MT, Sochacki M, Kowalski ML, Szkudlinska B, Zabrocki J, Di Lello P, Isernia C, Saviano M, Pedone C, Benedetti E
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Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2005; 80(2-3): 347-356.
Impact Factor: 2.425
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Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R
* Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (969 views)
Eur J Biochem (ISSN: 0014-2956, 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075.
Impact Factor: 2.849
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Benedetti E, Iacovino R, Pedone C, Rossi F, Saviano M, Isernia C, Montesarchio D, DeNapoli L, Piccialli G
β-Amino acid residues: Conformational characterization of an N- and C-protected homo-β-(S)-leucine (615 views)
Int J Pept Res Ther (ISSN: 0929-5666, 1573-3149), 1997 Jun; 4(3): 129-134.
Impact Factor: 0.557
View Export to BibTeX Export to EndNote
* The first example of a significant active site conformational rearrangement in a carbonic anhydrase-inhibitor adduct: The carbonic anhydrase I-topiramate complex (1754 views)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2010 Sep 7; 8(15): 3528-3533.
Impact Factor: 3.451
View Export to BibTeX Export to EndNote
La Mendola D, Magrì A, Campagna T, Campitiello MA, Raiola L, Isernia C, Hansson O, Bonomo RP, Rizzarelli E
* A doppel alpha-helix peptide fragment mimics the copper(II) interactions with the whole protein (1762 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2010 Jun 1; 16(21): 6212-6223.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Zubrzak P, Banas A, Kaczmarek K, Leplawy MT, Sochacki M, Kowalski ML, Szkudlinska B, Zabrocki J, Di Lello P, Isernia C, Saviano M, Pedone C, Benedetti E
* Analogues of cyclolinopeptide A containing alpha-hydroxymethyl amino acid residues (827 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2005; 80(2-3): 347-356.
Impact Factor: 2.425
View Export to BibTeX Export to EndNote
Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R
* Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (969 views)
Eur J Biochem (ISSN: 0014-2956, 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075.
Impact Factor: 2.849
View Export to BibTeX Export to EndNote
Benedetti E, Iacovino R, Pedone C, Rossi F, Saviano M, Isernia C, Montesarchio D, DeNapoli L, Piccialli G
β-Amino acid residues: Conformational characterization of an N- and C-protected homo-β-(S)-leucine (615 views)
Int J Pept Res Ther (ISSN: 0929-5666, 1573-3149), 1997 Jun; 4(3): 129-134.
Impact Factor: 0.557
View Export to BibTeX Export to EndNote
5 Records (5 excluding Abstracts).
Total impact factor: 14.758 (14.758 excluding Abstracts).
Total 5 year impact factor: 17.313 (17.313 excluding Abstracts).
Total impact factor: 14.758 (14.758 excluding Abstracts).
Total 5 year impact factor: 17.313 (17.313 excluding Abstracts).
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