A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library(483 views) Verdoliva A, Marasco D, De Capua A, Saporito A, Bellofiore P, Manfredi V, Fattorusso R, Pedone C, Ruvo M
Istituto di Biostrutture e Bioimmagini, CNR, Sezione Biostrutture, Via Mezzocannone 16, 80134, Napoli, Italy
TECNOGEN S.C.p.A. Località la Fagianeria, 81015, Piana Di Monte Verna, Caserta, Italy
Facoltà di Scienze Ambientali, Seconda Università di Napoli, Via Vivaldi 43, 81100 Caserta, Italy
TECNOGEN S. C. p. A. Localit la Fagianeria, 81015, Piana Di Monte Verna, Caserta, Italy
Facolt di Scienze Ambientali, Seconda Universit di Napoli, Via Vivaldi 43, 81100 Caserta, Italy
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A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library
By screening a synthetic peptide library of general formula (NH2-Cys1-X2-X3-X4)(2)-Lys-G1y-OH, a disulfide-bridged cyclic peptide, where X2-X3-X4 is the tripeptide Phe-His-His, has been selected as a ligand for immunoglobulin G (IgG). The peptide, after a preliminary chromatographic characterization, has proved useful as a new affinity ligand for the purification of polyclonol as well as monoclonal antibodies from biological fluids, with recovery yields of up to 90% (90% purity). The ligand is able to bind antibody fragments containing both Fab and Fc from different antibody isotypes, a fact suggesting the presence of at least two different antibody-binding sites. While the recognition site on Fab is unknown, comparative binding studies with Fc, in association with the striking similarities of the peptide (named Fc-receptor mimetic, FcRM) with a region of the human FcyRIII receptor, strongly indicate that the peptide could recognize a short amino acid stretch of the lower hinge region, which has a key role in autoimmune disease triggering. The unique properties make the ligand attractive for both the purification of antibody fragments and as a lead for the generation of Fc-receptor antagonists.
A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library
Bogdanovich S, Kim Y, Mizutani T, Yasuma R, Tudisco L, Cicatiello V, Bastos-carvalho A, Kerur N, Hirano Y, Baffi JZ, Tarallo V, Li S, Yasuma T, Arpitha P, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ruvo M, Sandomenico A, Nozaki M, Ijima R, Kaneko H, Ogura Y, Terasaki H, Ambati BK, Leusen JH, Langdon WY, Clark MR, Armour KL, Bruhns P, Verbeek JS, Gelfand BD, De Falco S, Ambati J * Human IgG1 antibodies suppress angiogenesis in a target-independent manner(557 views) Signal Transduct Target Ther (ISSN: 2059-3635print), 2016; 1: N/D-N/D. Impact Factor:0 ViewExport to BibTeXExport to EndNote