Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries (431 views visite)
Chem Biol Drug Des Chemical Biology And Drug Design (ISSN: 1747-0277, 1747-0285), 2011 May; 77(5): 319-327.
Export to Esporta in BibTeX Export to Esporta in EndNote
Paper type Tipo di articolo: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.282, 5-year impact factor Impact factor a 5 anni: 2.477
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-79954618035&partnerID=40&md5=bd71e2be486cbcd993494354f9485d5f
Keywords Parole chiave: Competitive Assays, Enzyme-Linked Immunosorbent Assay, Simplified Combinatorial Peptide Libraries, Surface Plasmon Resonance, Glutamylglycylglycyltyrosylaspartylhistidyltyrosine, Glutamylglycylglycyltyrosylprolylhistidyltyrosine, Lysylglycylglycylmethionylalanylmethionylleucine, Lysylglycylglycylserylprolylphenylalanylglutamine, Lysylglycylglycylserylprolylphenylalanyltyrosine, Phospholipase D1, Phospholipase D1 D4 Alpha, Phospholipase D1 Ped, Unclassified Drug, Article, Combinatorial Library, Controlled Study, Enzyme Linked Immunosorbent Assay, Insulin Resistance, Non Insulin Dependent Diabetes Mellitus, Nonhuman, Priority Journal, Protein Interaction, Screening, Automation, Laboratory, Binding, Cloning, Molecular, Combinatorial Chemistry Techniques, Type 2, Escherichia Coli, High-Throughput Screening Assays, Inhibitory Concentration 50, Intracellular Signaling Peptides And Proteins, Kinetics, Phosphoproteins, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Structure-Activity Relationship,
Affiliations Affiliazioni:
*** IBB - CNR ***
Department of Biological Sciences, School of Biotechnological Sciences, University of Naples Federico II, Via Mezzocannone, 16, 80134, Naples, Italy
Department of Pharmaceutical and Toxicological Chemistry, University of Naples ''Federico II'', Via Montesano 49, Naples, Italy
Institute of Biostructures and Bioimaging - CNR, Via Mezzocannone 16, 80134, Naples, Italy
References Riferimenti:
Scior, T., Bernard, P., Medina-Franco, J.L., Maggiora, G.M., Large compound databases for structure-activity relationships studies in drug discovery (2007) Mini Rev Med Chem, 7, pp. 851-86
Frank, R., Heikens, W., Heisterberg-Moutsis, G., Blocker, H., A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports (1983) Nucleic Acids Res, 11, pp. 4365-4377
Houghten, R.A., General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids (1985) Proc Natl Acad Sci USA, 82, pp. 5131-5135
Balakin, K.V., Kozintsev, A.V., Kiselyov, A.S., Savchuk, N.P., Rational design approaches to chemical libraries for hit identification (2006) Curr Drug Discov Technol, 3, pp. 49-65
Ecker, D.J., Crooke, S.T., Combinatorial drug discovery: which methods will produce the greatest value? (1995) Biotechnology (NY), 13, pp. 351-360
Kennedy, J.P., Williams, L., Bridges, T.M., Daniels, R.N., Weaver, D., Lindsley, C.W., Application of combinatorial chemistry science on modern drug discovery (2008) J Comb Chem, 10, pp. 345-354
Pinilla, C., Appel, J.R., Blanc, P., Houghten, R.A., Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries (1992) BioTechniques, 13, pp. 901-905
Betzi, S., Restouin, A., Opi, S., Arold, S.T., Parrot, I., Guerlesquin, F., Morelli, X., Collette, Y., Protein-protein interaction inhibition (2P2I) combining high throughput and virtual screening: application to the HIV-1 Nef protein (2007) Proc Natl Acad Sci USA, 104, pp. 19256-19261
Wells, J.A., McClendon, C.L., Reaching for high-hanging fruit in drug discovery at protein-protein interfaces (2007) Nature, 450, pp. 1001-1009
Chene, P., Drugs targeting protein-protein interactions (2006) ChemMedChem, 1, pp. 400-411
Shultz, M.D., Ham, Y.W., Lee, S.G., Davis, D.A., Brown, C., Chmielewski, J., Small-molecule dimerization inhibitors of wild-type and mutant HIV protease: a focused library approach (2004) J Am Chem Soc, 126, pp. 9886-9887
Zhao, H.F., Kiyota, T., Chowdhury, S., Purisima, E., Banville, D., Konishi, Y., Shen, S.H., A mammalian genetic system to screen for small molecules capable of disrupting protein-protein interactions (2004) Anal Chem, 76, pp. 2922-2927
Ponticelli, S., Marasco, D., Tarallo, V., Albuquerque, R.J., Mitola, S., Takeda, A., Stassen, J.M., De Falco, S., Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1 (2008) J Biol Chem, 283, pp. 34250-34259
Sillerud, L.O., Larson, R.S., Design and structure of peptide and peptidomimetic antagonists of protein-protein interaction (2005) Curr Protein Pept Sci, 6, pp. 151-169
Martin, E.J., Blaney, J.M., Siani, M.A., Spellmeyer, D.C., Wong, A.K., Moos, W.H., Measuring diversity: experimental design of combinatorial libraries for drug discovery (1995) J Med Chem, 38, pp. 1431-1436
Marasco, D., Perretta, G., Sabatella, M., Ruvo, M., Past and future perspectives of synthetic peptide libraries (2008) Curr Protein Pept Sci, 9, pp. 447-467
He, J., Eckert, R., Pharm, T., Simanian, M.D., Hu, C., Yarbrough, D.K., Qi, F., Shi, W., Novel synthetic antimicrobial peptides against Streptococcus mutans (2007) Antimicrob Agents Chemother, 51, pp. 1351-1358
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO J, 17, pp. 3858-3866
Fiory, F., Formisano, P., Perruolo, G., Beguinot, F., Frontiers: PED/PEA-15, a multifunctional protein controlling cell survival and glucose metabolism (2009) Am J Physiol Endocrinol Metab, 297, pp. E592-E601
Viparelli, F., Cassese, A., Doti, N., Paturzo, F., Marasco, D., Dathan, N.A., Monti, S.M., Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (2008) J Biol Chem, 283, pp. 21769-21778
Doti, N., Cassese, A., Marasco, D., Paturzo, F., Sabatella, M., Viparelli, F., Dathan, N., Ruvo, M., Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (2010) Mol Biosyst, 6, pp. 2039-2048
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) Int J Pept Protein Res, 35, pp. 161-214
Geysen, H.M., Rodda, S.J., Mason, T.J., A priori delineation of a peptide which mimics a discontinuous antigenic determinant (1986) Mol Immunol, 23, pp. 709-715
Ruvo, M., Scardino, P., Cassani, G., Fassina, G., Facile manual synthesis of peptide libraries (1994) Protein Pept Lett, 1, pp. 187-192
Johnsson, B., Lofas, S., Lindquist, G., Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors (1991) Anal Biochem, 198, pp. 268-277
Flier, J.S., Insulin receptors and insulin resistance (1983) Annu Rev Med, 34, pp. 145-160
Cheng, A.C., Coleman, R.G., Smyth, K.T., Cao, Q., Soulard, P., Caffrey, D.R., Salzberg, A.C., Huang, E.S., Structure-based maximal affinity model predicts small-molecule druggability (2007) Nat Biotechnol, 25, pp. 71-75
Arkin, M.R., Randal, M., DeLano, W.L., Hyde, J., Luong, T.N., Oslob, J.D., Raphael, D.R., Braisted, A.C., Binding of small molecules to an adaptive protein-protein interface (2003) Proc Natl Acad Sci USA, 100, pp. 1603-1608
Sidhu, S.S., Fairbrother, W.J., Deshayes, K., Exploring protein-protein interactions with phage display (2003) Chembiochem, 4, pp. 14-25
Pillutla, R.C., Hsiao, K.C., Beasley, J.R., Brandt, J., Ostergaard, S., Hansen, P.H., Spetzler, J.C., Goldstein, N.I., Peptides identify the critical hotspots involved in the biological activation of the insulin receptor (2002) J Biol Chem, 277, pp. 22590-22594
Nakamura, G.R., Reynolds, M.E., Chen, Y.M., Starovasnik, M.A., Lowman, H.B., Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor (2002) Proc Natl Acad Sci USA, 99, pp. 1303-1308
Besterman, J.M., Duronio, V., Cuatrecasas, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc Natl Acad Sci USA, 83, pp. 6785-6789
Sung, T.C., Zhang, Y., Morris, A.J., Frohman, M.A., Structural analysis of human phospholipase D1 (1999) J Biol Chem, 274, pp. 3659-3666
Xie, Z., Ho, W.T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D is required for catalytic activity (1998) J Biol Chem, 273, pp. 34679-34682
Houghten, R. A., General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids (1985) Proc Natl Acad Sci USA, 82, pp. 5131-5135
Balakin, K. V., Kozintsev, A. V., Kiselyov, A. S., Savchuk, N. P., Rational design approaches to chemical libraries for hit identification (2006) Curr Drug Discov Technol, 3, pp. 49-65
Ecker, D. J., Crooke, S. T., Combinatorial drug discovery: which methods will produce the greatest value? (1995) Biotechnology (NY), 13, pp. 351-360
Kennedy, J. P., Williams, L., Bridges, T. M., Daniels, R. N., Weaver, D., Lindsley, C. W., Application of combinatorial chemistry science on modern drug discovery (2008) J Comb Chem, 10, pp. 345-354
Wells, J. A., McClendon, C. L., Reaching for high-hanging fruit in drug discovery at protein-protein interfaces (2007) Nature, 450, pp. 1001-1009
Shultz, M. D., Ham, Y. W., Lee, S. G., Davis, D. A., Brown, C., Chmielewski, J., Small-molecule dimerization inhibitors of wild-type and mutant HIV protease: a focused library approach (2004) J Am Chem Soc, 126, pp. 9886-9887
Zhao, H. F., Kiyota, T., Chowdhury, S., Purisima, E., Banville, D., Konishi, Y., Shen, S. H., A mammalian genetic system to screen for small molecules capable of disrupting protein-protein interactions (2004) Anal Chem, 76, pp. 2922-2927
Sillerud, L. O., Larson, R. S., Design and structure of peptide and peptidomimetic antagonists of protein-protein interaction (2005) Curr Protein Pept Sci, 6, pp. 151-169
Martin, E. J., Blaney, J. M., Siani, M. A., Spellmeyer, D. C., Wong, A. K., Moos, W. H., Measuring diversity: experimental design of combinatorial libraries for drug discovery (1995) J Med Chem, 38, pp. 1431-1436
Fields, G. B., Noble, R. L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) Int J Pept Protein Res, 35, pp. 161-214
Geysen, H. M., Rodda, S. J., Mason, T. J., A priori delineation of a peptide which mimics a discontinuous antigenic determinant (1986) Mol Immunol, 23, pp. 709-715
Flier, J. S., Insulin receptors and insulin resistance (1983) Annu Rev Med, 34, pp. 145-160
Cheng, A. C., Coleman, R. G., Smyth, K. T., Cao, Q., Soulard, P., Caffrey, D. R., Salzberg, A. C., Huang, E. S., Structure-based maximal affinity model predicts small-molecule druggability (2007) Nat Biotechnol, 25, pp. 71-75
Arkin, M. R., Randal, M., DeLano, W. L., Hyde, J., Luong, T. N., Oslob, J. D., Raphael, D. R., Braisted, A. C., Binding of small molecules to an adaptive protein-protein interface (2003) Proc Natl Acad Sci USA, 100, pp. 1603-1608
Sidhu, S. S., Fairbrother, W. J., Deshayes, K., Exploring protein-protein interactions with phage display (2003) Chembiochem, 4, pp. 14-25
Pillutla, R. C., Hsiao, K. C., Beasley, J. R., Brandt, J., Ostergaard, S., Hansen, P. H., Spetzler, J. C., Goldstein, N. I., Peptides identify the critical hotspots involved in the biological activation of the insulin receptor (2002) J Biol Chem, 277, pp. 22590-22594
Nakamura, G. R., Reynolds, M. E., Chen, Y. M., Starovasnik, M. A., Lowman, H. B., Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor (2002) Proc Natl Acad Sci USA, 99, pp. 1303-1308
Besterman, J. M., Duronio, V., Cuatrecasas, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc Natl Acad Sci USA, 83, pp. 6785-6789
Sung, T. C., Zhang, Y., Morris, A. J., Frohman, M. A., Structural analysis of human phospholipase D1 (1999) J Biol Chem, 274, pp. 3659-3666
Xie, Z., Ho, W. T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D is required for catalytic activity (1998) J Biol Chem, 273, pp. 34679-34682
Chem Biol Drug Des Chemical Biology And Drug Design (ISSN: 1747-0277, 1747-0285), 2011 May; 77(5): 319-327.
Export to Esporta in BibTeX Export to Esporta in EndNote
Paper type Tipo di articolo: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.282, 5-year impact factor Impact factor a 5 anni: 2.477
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-79954618035&partnerID=40&md5=bd71e2be486cbcd993494354f9485d5f
Keywords Parole chiave: Competitive Assays, Enzyme-Linked Immunosorbent Assay, Simplified Combinatorial Peptide Libraries, Surface Plasmon Resonance, Glutamylglycylglycyltyrosylaspartylhistidyltyrosine, Glutamylglycylglycyltyrosylprolylhistidyltyrosine, Lysylglycylglycylmethionylalanylmethionylleucine, Lysylglycylglycylserylprolylphenylalanylglutamine, Lysylglycylglycylserylprolylphenylalanyltyrosine, Phospholipase D1, Phospholipase D1 D4 Alpha, Phospholipase D1 Ped, Unclassified Drug, Article, Combinatorial Library, Controlled Study, Enzyme Linked Immunosorbent Assay, Insulin Resistance, Non Insulin Dependent Diabetes Mellitus, Nonhuman, Priority Journal, Protein Interaction, Screening, Automation, Laboratory, Binding, Cloning, Molecular, Combinatorial Chemistry Techniques, Type 2, Escherichia Coli, High-Throughput Screening Assays, Inhibitory Concentration 50, Intracellular Signaling Peptides And Proteins, Kinetics, Phosphoproteins, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Structure-Activity Relationship,
Affiliations Affiliazioni:
*** IBB - CNR ***
Department of Biological Sciences, School of Biotechnological Sciences, University of Naples Federico II, Via Mezzocannone, 16, 80134, Naples, Italy
Department of Pharmaceutical and Toxicological Chemistry, University of Naples ''Federico II'', Via Montesano 49, Naples, Italy
Institute of Biostructures and Bioimaging - CNR, Via Mezzocannone 16, 80134, Naples, Italy
References Riferimenti:
Scior, T., Bernard, P., Medina-Franco, J.L., Maggiora, G.M., Large compound databases for structure-activity relationships studies in drug discovery (2007) Mini Rev Med Chem, 7, pp. 851-86
Frank, R., Heikens, W., Heisterberg-Moutsis, G., Blocker, H., A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports (1983) Nucleic Acids Res, 11, pp. 4365-4377
Houghten, R.A., General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids (1985) Proc Natl Acad Sci USA, 82, pp. 5131-5135
Balakin, K.V., Kozintsev, A.V., Kiselyov, A.S., Savchuk, N.P., Rational design approaches to chemical libraries for hit identification (2006) Curr Drug Discov Technol, 3, pp. 49-65
Ecker, D.J., Crooke, S.T., Combinatorial drug discovery: which methods will produce the greatest value? (1995) Biotechnology (NY), 13, pp. 351-360
Kennedy, J.P., Williams, L., Bridges, T.M., Daniels, R.N., Weaver, D., Lindsley, C.W., Application of combinatorial chemistry science on modern drug discovery (2008) J Comb Chem, 10, pp. 345-354
Pinilla, C., Appel, J.R., Blanc, P., Houghten, R.A., Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries (1992) BioTechniques, 13, pp. 901-905
Betzi, S., Restouin, A., Opi, S., Arold, S.T., Parrot, I., Guerlesquin, F., Morelli, X., Collette, Y., Protein-protein interaction inhibition (2P2I) combining high throughput and virtual screening: application to the HIV-1 Nef protein (2007) Proc Natl Acad Sci USA, 104, pp. 19256-19261
Wells, J.A., McClendon, C.L., Reaching for high-hanging fruit in drug discovery at protein-protein interfaces (2007) Nature, 450, pp. 1001-1009
Chene, P., Drugs targeting protein-protein interactions (2006) ChemMedChem, 1, pp. 400-411
Shultz, M.D., Ham, Y.W., Lee, S.G., Davis, D.A., Brown, C., Chmielewski, J., Small-molecule dimerization inhibitors of wild-type and mutant HIV protease: a focused library approach (2004) J Am Chem Soc, 126, pp. 9886-9887
Zhao, H.F., Kiyota, T., Chowdhury, S., Purisima, E., Banville, D., Konishi, Y., Shen, S.H., A mammalian genetic system to screen for small molecules capable of disrupting protein-protein interactions (2004) Anal Chem, 76, pp. 2922-2927
Ponticelli, S., Marasco, D., Tarallo, V., Albuquerque, R.J., Mitola, S., Takeda, A., Stassen, J.M., De Falco, S., Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1 (2008) J Biol Chem, 283, pp. 34250-34259
Sillerud, L.O., Larson, R.S., Design and structure of peptide and peptidomimetic antagonists of protein-protein interaction (2005) Curr Protein Pept Sci, 6, pp. 151-169
Martin, E.J., Blaney, J.M., Siani, M.A., Spellmeyer, D.C., Wong, A.K., Moos, W.H., Measuring diversity: experimental design of combinatorial libraries for drug discovery (1995) J Med Chem, 38, pp. 1431-1436
Marasco, D., Perretta, G., Sabatella, M., Ruvo, M., Past and future perspectives of synthetic peptide libraries (2008) Curr Protein Pept Sci, 9, pp. 447-467
He, J., Eckert, R., Pharm, T., Simanian, M.D., Hu, C., Yarbrough, D.K., Qi, F., Shi, W., Novel synthetic antimicrobial peptides against Streptococcus mutans (2007) Antimicrob Agents Chemother, 51, pp. 1351-1358
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO J, 17, pp. 3858-3866
Fiory, F., Formisano, P., Perruolo, G., Beguinot, F., Frontiers: PED/PEA-15, a multifunctional protein controlling cell survival and glucose metabolism (2009) Am J Physiol Endocrinol Metab, 297, pp. E592-E601
Viparelli, F., Cassese, A., Doti, N., Paturzo, F., Marasco, D., Dathan, N.A., Monti, S.M., Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (2008) J Biol Chem, 283, pp. 21769-21778
Doti, N., Cassese, A., Marasco, D., Paturzo, F., Sabatella, M., Viparelli, F., Dathan, N., Ruvo, M., Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (2010) Mol Biosyst, 6, pp. 2039-2048
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) Int J Pept Protein Res, 35, pp. 161-214
Geysen, H.M., Rodda, S.J., Mason, T.J., A priori delineation of a peptide which mimics a discontinuous antigenic determinant (1986) Mol Immunol, 23, pp. 709-715
Ruvo, M., Scardino, P., Cassani, G., Fassina, G., Facile manual synthesis of peptide libraries (1994) Protein Pept Lett, 1, pp. 187-192
Johnsson, B., Lofas, S., Lindquist, G., Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors (1991) Anal Biochem, 198, pp. 268-277
Flier, J.S., Insulin receptors and insulin resistance (1983) Annu Rev Med, 34, pp. 145-160
Cheng, A.C., Coleman, R.G., Smyth, K.T., Cao, Q., Soulard, P., Caffrey, D.R., Salzberg, A.C., Huang, E.S., Structure-based maximal affinity model predicts small-molecule druggability (2007) Nat Biotechnol, 25, pp. 71-75
Arkin, M.R., Randal, M., DeLano, W.L., Hyde, J., Luong, T.N., Oslob, J.D., Raphael, D.R., Braisted, A.C., Binding of small molecules to an adaptive protein-protein interface (2003) Proc Natl Acad Sci USA, 100, pp. 1603-1608
Sidhu, S.S., Fairbrother, W.J., Deshayes, K., Exploring protein-protein interactions with phage display (2003) Chembiochem, 4, pp. 14-25
Pillutla, R.C., Hsiao, K.C., Beasley, J.R., Brandt, J., Ostergaard, S., Hansen, P.H., Spetzler, J.C., Goldstein, N.I., Peptides identify the critical hotspots involved in the biological activation of the insulin receptor (2002) J Biol Chem, 277, pp. 22590-22594
Nakamura, G.R., Reynolds, M.E., Chen, Y.M., Starovasnik, M.A., Lowman, H.B., Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor (2002) Proc Natl Acad Sci USA, 99, pp. 1303-1308
Besterman, J.M., Duronio, V., Cuatrecasas, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc Natl Acad Sci USA, 83, pp. 6785-6789
Sung, T.C., Zhang, Y., Morris, A.J., Frohman, M.A., Structural analysis of human phospholipase D1 (1999) J Biol Chem, 274, pp. 3659-3666
Xie, Z., Ho, W.T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D is required for catalytic activity (1998) J Biol Chem, 273, pp. 34679-34682
Houghten, R. A., General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids (1985) Proc Natl Acad Sci USA, 82, pp. 5131-5135
Balakin, K. V., Kozintsev, A. V., Kiselyov, A. S., Savchuk, N. P., Rational design approaches to chemical libraries for hit identification (2006) Curr Drug Discov Technol, 3, pp. 49-65
Ecker, D. J., Crooke, S. T., Combinatorial drug discovery: which methods will produce the greatest value? (1995) Biotechnology (NY), 13, pp. 351-360
Kennedy, J. P., Williams, L., Bridges, T. M., Daniels, R. N., Weaver, D., Lindsley, C. W., Application of combinatorial chemistry science on modern drug discovery (2008) J Comb Chem, 10, pp. 345-354
Wells, J. A., McClendon, C. L., Reaching for high-hanging fruit in drug discovery at protein-protein interfaces (2007) Nature, 450, pp. 1001-1009
Shultz, M. D., Ham, Y. W., Lee, S. G., Davis, D. A., Brown, C., Chmielewski, J., Small-molecule dimerization inhibitors of wild-type and mutant HIV protease: a focused library approach (2004) J Am Chem Soc, 126, pp. 9886-9887
Zhao, H. F., Kiyota, T., Chowdhury, S., Purisima, E., Banville, D., Konishi, Y., Shen, S. H., A mammalian genetic system to screen for small molecules capable of disrupting protein-protein interactions (2004) Anal Chem, 76, pp. 2922-2927
Sillerud, L. O., Larson, R. S., Design and structure of peptide and peptidomimetic antagonists of protein-protein interaction (2005) Curr Protein Pept Sci, 6, pp. 151-169
Martin, E. J., Blaney, J. M., Siani, M. A., Spellmeyer, D. C., Wong, A. K., Moos, W. H., Measuring diversity: experimental design of combinatorial libraries for drug discovery (1995) J Med Chem, 38, pp. 1431-1436
Fields, G. B., Noble, R. L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) Int J Pept Protein Res, 35, pp. 161-214
Geysen, H. M., Rodda, S. J., Mason, T. J., A priori delineation of a peptide which mimics a discontinuous antigenic determinant (1986) Mol Immunol, 23, pp. 709-715
Flier, J. S., Insulin receptors and insulin resistance (1983) Annu Rev Med, 34, pp. 145-160
Cheng, A. C., Coleman, R. G., Smyth, K. T., Cao, Q., Soulard, P., Caffrey, D. R., Salzberg, A. C., Huang, E. S., Structure-based maximal affinity model predicts small-molecule druggability (2007) Nat Biotechnol, 25, pp. 71-75
Arkin, M. R., Randal, M., DeLano, W. L., Hyde, J., Luong, T. N., Oslob, J. D., Raphael, D. R., Braisted, A. C., Binding of small molecules to an adaptive protein-protein interface (2003) Proc Natl Acad Sci USA, 100, pp. 1603-1608
Sidhu, S. S., Fairbrother, W. J., Deshayes, K., Exploring protein-protein interactions with phage display (2003) Chembiochem, 4, pp. 14-25
Pillutla, R. C., Hsiao, K. C., Beasley, J. R., Brandt, J., Ostergaard, S., Hansen, P. H., Spetzler, J. C., Goldstein, N. I., Peptides identify the critical hotspots involved in the biological activation of the insulin receptor (2002) J Biol Chem, 277, pp. 22590-22594
Nakamura, G. R., Reynolds, M. E., Chen, Y. M., Starovasnik, M. A., Lowman, H. B., Stable "zeta" peptides that act as potent antagonists of the high-affinity IgE receptor (2002) Proc Natl Acad Sci USA, 99, pp. 1303-1308
Besterman, J. M., Duronio, V., Cuatrecasas, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc Natl Acad Sci USA, 83, pp. 6785-6789
Sung, T. C., Zhang, Y., Morris, A. J., Frohman, M. A., Structural analysis of human phospholipase D1 (1999) J Biol Chem, 274, pp. 3659-3666
Xie, Z., Ho, W. T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D is required for catalytic activity (1998) J Biol Chem, 273, pp. 34679-34682
Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries
Most biological processes involve permanent and temporary interactions between different proteins: protein complexes often play key roles in human diseases and, as a consequence, molecules that prevent protein-protein interactions can be potential new therapeutic agents to treat diseases. Here, we describe a simplified approach by which small synthetic peptide libraries were screened to identify the inhibitors of the complex between phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes15 (PED/PEA15) and D4α, a functional domain of the phospholipase D1, that is involved in the molecular mechanisms of insulin resistance occurring in type 2 diabetes. By using an enzyme-linked immunosorbent assay (ELISA)-based screening, performed on a fully automated platform, we analyzed two simplified peptide libraries in a positional scanning format. This screening led to the identification of small peptides able to inhibit PED/PEA15-D4α interaction. The selection of inhibitors was carried out employing combined competitive and direct experiments, through ELISA and surface plasmon resonance techniques, providing peptides with IC 50 values in the micromolar range. Our results showed that the protein complex PED/PEA15-D4α is susceptible to peptides having H-donor groups and aromatic rings on specific positions. These small sequences can be considered as promising scaffolds that could be converted into higher-affinity inhibitor compounds. By a simplified approach small synthetic peptide libraries were screened to identify inhibitors of the complex between PED/PEA15 and D4α that is involved in molecular mechanisms of insulin resistance in type 2 diabetes. Small peptide sequences having H-donor groups and aromatic rings on specific positions are able to inhibit the complex and are promising scaffolds that could be converted into higher-affinity inhibitor compounds. © 2011 John Wiley & Sons A/S.
Most biological processes involve permanent and temporary interactions between different proteins: protein complexes often play key roles in human diseases and, as a consequence, molecules that prevent protein-protein interactions can be potential new therapeutic agents to treat diseases. Here, we describe a simplified approach by which small synthetic peptide libraries were screened to identify the inhibitors of the complex between phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes15 (PED/PEA15) and D4α, a functional domain of the phospholipase D1, that is involved in the molecular mechanisms of insulin resistance occurring in type 2 diabetes. By using an enzyme-linked immunosorbent assay (ELISA)-based screening, performed on a fully automated platform, we analyzed two simplified peptide libraries in a positional scanning format. This screening led to the identification of small peptides able to inhibit PED/PEA15-D4α interaction. The selection of inhibitors was carried out employing combined competitive and direct experiments, through ELISA and surface plasmon resonance techniques, providing peptides with IC 50 values in the micromolar range. Our results showed that the protein complex PED/PEA15-D4α is susceptible to peptides having H-donor groups and aromatic rings on specific positions. These small sequences can be considered as promising scaffolds that could be converted into higher-affinity inhibitor compounds. By a simplified approach small synthetic peptide libraries were screened to identify inhibitors of the complex between PED/PEA15 and D4α that is involved in molecular mechanisms of insulin resistance in type 2 diabetes. Small peptide sequences having H-donor groups and aromatic rings on specific positions are able to inhibit the complex and are promising scaffolds that could be converted into higher-affinity inhibitor compounds. © 2011 John Wiley & Sons A/S.
Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries
Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries
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Viparelli F, Cassese A, Doti N, Paturzo F, Marasco D, Dathan NA, Monti SM, Basile G, Ungaro P, Sabatella M, Miele C, Teperino R, Consiglio E, Pedone C, Beguinot F, Formisano P, Ruvo M
* Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (389 visite)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Sep 1; 283(31): 21769-21778.
Impact Factor: 5.52
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D'Agata R, Corradini R, Grasso G, Marchelli R, Spoto G
* Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging (366 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2008 Sep 1; 9(13): 2067-2070.
Impact Factor: 3.322
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Grasso G, Rizzarelli E, Spoto G
* How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin (323 visite)
Bba-Gen Subjects (ISSN: 0006-3002, 1570-9639, 0925-4439), 2008 Jul; 1784(7-8): 1122-1126.
Impact Factor: 2.233
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Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (408 visite)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
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Cigliano L, Maresca B, Salvatore A, Nino M, Monfrecola G, Ayala F, Carlucci A, Pugliese RC, Pedone C, Abrescia P
* Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity (339 visite)
Journal Of The European Academy Of Dermatology And Venereology (ISSN: 1468-3083, 0926-9959), 2008 Apr; 22(4): 417-425.
Impact Factor: 2.276
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Gallotta G, Palmieri V, Piedimonte V, Rendina D, De Bonis S, Russo V, Celentano A, Di Minno M, Postiglione A, Di Minno G
* Increased troponin I predicts in-hospital occurrence of hemodynamic instability in patients with sub-massive or non-massive pulmonary embolism independent to clinical, echocardiographic and laboratory information (254 visite)
Int J Cardiol (ISSN: 0167-5273, 1874-1754electronic, 0167-5273linking), 2008 Mar 14; 124(3): 351-357.
Impact Factor: 3.121
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Migliore T, Parrella LS, Caputi A, Silvestri N, Romano R, Pace L, Imbriaco M, Losi MA, Betocchi S
* Pathogenesis of hypertrophic cardiomyopathy. Impact of growth factors on left ventricular anatomy (332 visite)
Minerva Cardioangiol (ISSN: 0026-4725), 2008 Feb; 56(1): 13-20.
Impact Factor: 0.481
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Rossi M, Ruvo M, Marasco D, Colombo M, Cassani G, Verdoliva A
* Anti-allergic properties of a new all-D synthetic immunoglobulin-binding peptide (402 visite)
Mol Immunol (ISSN: 0161-5890), 2008 Jan; 45(1): 226-234.
Impact Factor: 3.555
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Zanoli L, Dagata R, Spoto G
* Surface plasmon-based optical detection of DNA peptide nucleic acids (244 visite)
Minerva Biotecnol (ISSN: 1120-4826), 2008; 20(4): 165-174.
Impact Factor: 0.1
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Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111) (306 visite)
Journal Of The American Society For Mass Spectrometry (ISSN: 1044-0305), 2007 May; 18(5): 961-969.
Impact Factor: 3.664
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Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* In situ AP/MALDI-MS characterization of anchored matrix metalloproteinases (381 visite)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2006 Dec; 41(12): 1561-1569.
Impact Factor: 2.945
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D'Agata R, Grasso G, Iacono G, Spoto G, Vecchio G
* Lectin recognition of a new SOD mimic bioconjugate studied with surface plasmon resonance imaging (273 visite)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2006 Feb 21; 4(4): 610-612.
Impact Factor: 2.874
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Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (478 visite)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
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Verdoliva A, Marasco D, De Capua A, Saporito A, Bellofiore P, Manfredi V, Fattorusso R, Pedone C, Ruvo M
* A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library (433 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2005 Jul; 6(7): 1242-1253.
Impact Factor: 3.94
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Arena G, Contino A, D'Agata R, Sgarlata C, Spoto G
* Ordered anchored cavities at work: A new and rapid SPR-based method for the detection of trace amounts of Cs+ (387 visite)
New J Chem (ISSN: 1144-0546), 2005; 29(11): 1393-1395.
Impact Factor: 2.574
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Rossi M, Manfredi V, Ruvo M, Fassina G, Verdoliva A
* Sequence-simplification and chimeric assembly: New models of peptide antigen modification (358 visite)
Mol Immunol (ISSN: 0161-5890), 2002 Nov; 39(7-8): 443-451.
Impact Factor: 2.414
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De Falco S, Ruvo M, Verdoliva A, Scarallo A, Raimondo D, Raucci A, Fassina G
* N-terminal myristylation of HBV preS1 domain enhances receptor recognition (391 visite)
J Pept Res (ISSN: 1397-002x, 1397-002xlinking), 2001 May; 57(5): 390-400.
Impact Factor: 1.522
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Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S
* Fusion in Coq (424 visite)
Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596.
Impact Factor: 0.415
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Monti SM, Fogliano V, Randazzo G, Peluso G, Logrieco A, Ritieni A
Polyclonal antibodies against fusaproliferin (276 visite)
Can J Microbiol (ISSN: 0008-4166), 1999 Jan; 45(1): 45-50.
Impact Factor: 1.072
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Bruckmann C, Tamburri S, De Lorenzi V, Doti N, Monti A, Mathiasen L, Cattaneo A, Ruvo M, Bachi A, Blasi F
* Mapping the native interaction surfaces of PREP1 with PBX1 by cross-linking mass-spectrometry and mutagenesis (43 visite)
Sci Rep (ISSN: 2045-2322linking), 2020 Oct 8; 10(1): 16809-16809.
Impact Factor: 3.998
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De Luca S, Verdoliva V, Saviano M, Fattorusso R, Diana D
* SPR and NMR characterization of the molecular interaction between A9 peptide and a model system of HER2 receptor: A fragment approach for selecting peptide structures specific for their target (98 visite)
J Pept Sci (ISSN: 1075-2617linking), 2020 Feb; 26(2): e3231-e3231.
Impact Factor: 1.877
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La Manna S, Scognamiglio PL, Di Natale C, Leone M, Mercurio FA, Malfitano AM, Cianfarani F, Madonna S, Caravella S, Albanesi C, Novellino E, Marasco D
* Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces (369 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2017 May 04; 138: 106-115.
Impact Factor: 3.188
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Leiria Campo V, Riul TB, Oliveira Bortot L, Martins-teixeira MB, Fiori Marchiori M, Iaccarino E, Ruvo M, Dias-baruffi M, Carvalho I
* A Synthetic MUC1 Glycopeptide Bearing betaGalNAc-Thr as a Tn Antigen Isomer Induces the Production of Antibodies against Tumor Cells (794 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2017 Mar 16; 18(6): 527-538.
Impact Factor: 2.774
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Accardo A, Arena F, Gianolio E, Marasco D, Ringhieri P, Boffa C, Bardini P, Aime S, Morelli G
* Diolein based nanostructures as targeted theranostics (405 visite)
J Biomed Nanotechnol (ISSN: 1550-7033), 2016; 12(5): 1076-1088.
Impact Factor: 4.521
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Palladino P, Aura AM, Spoto G
* Surface plasmon resonance for the label-free detection of Alzheimer's β-amyloid peptide aggregation (467 visite)
Anal Bioanal Chem (ISSN: 1618-2642), 2016; 408(3): 849-854.
Impact Factor: 3.431
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Foca A, Sanguigno L, Foca G, Strizzi L, Iannitti R, Palumbo R, Hendrix MJ, Leonardi A, Ruvo M, Sandomenico A
* New Anti-Nodal Monoclonal Antibodies Targeting the Nodal Pre-Helix Loop Involved in Cripto-1 Binding (615 visite) (PDF 252 visite)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2015 Sep 7; 16(9): 21342-21362.
Impact Factor: 3.257
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Calvanese L, Sandomenico A, Caporale A, Foca A, Foca G, D'Auria G, Falcigno L, Ruvo M
* Conformational features and binding affinities to Cripto, ALK7 and ALK4 of Nodal synthetic fragments (399 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617linking), 2015 Jan 15; 21(4): 283-293.
Impact Factor: 1.951
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GrassoG, Satriano C, Milardi D
* A neglected modulator of insulin-degrading enzyme activity and conformation: The pH (354 visite)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2015; 203-204: 33-40.
Impact Factor: 2.363
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Lanza V, D'Agata R, Iacono G, Bellia F, Spoto G, Vecchio G
* Cyclam glycoconjugates as lectin ligands and protective agents of metal-induced amyloid aggregation (495 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2015; 153: 377-382.
Impact Factor: 3.205
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Falcigno L, D'Auria G, Calvanese L, Marasco D, Iacobelli R, Scognamiglio PL, Brun P, Danesin R, Pasqualin M, Castagliuolo I, Dettin M
* Osteogenic properties of a short BMP-2 chimera peptide (450 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617linking), 2015; 21(9): 700-709.
Impact Factor: 1.951
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Marasco D, Messori L, Marzo T, Merlino A
* Oxaliplatin vs. cisplatin: Competition experiments on their binding to lysozyme (689 visite)
Dalton T (ISSN: 1477-9226, 1477-9234, 1477-9226linking), 2015; 44(22): 10392-10398.
Impact Factor: 4.177
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Mercurio FA, Di Natale C, Pirone L, Scognamiglio PL, Marasco D, Pedone EM, Saviano M, Leone M
* Peptide Fragments of Odin-Sam1: Conformational Analysis and Interaction Studies with EphA2-Sam (431 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2015; 16(11): 1629-1636.
Impact Factor: 2.85
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Altieri F, Di Stadio CS, Severino V, Sandomenico A, Minopoli G, Miselli G, Di Maro A, Ruvo M, Chambery A, Quagliariello V, Masullo M, Rippa E, Arcari P
* Anti-amyloidogenic property of human gastrokine (467 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Nov; 106C: 91-100.
Impact Factor: 2.963
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Calvanese L, Falcigno L, Maglione C, Marasco D, Ruggiero A, Squeglia F, Berisio R, D'Auria G
* Structural and binding properties of the PASTA domain of PonA2, a key penicillin binding protein from Mycobacterium tuberculosis (434 visite)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2014 Jul; 101(7): 712-719.
Impact Factor: 2.385
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Scognamiglio PL, Di Natale C, Leone M, Poletto M, Vitagliano L, Tell G, Marasco D
* G-quadruplex DNA recognition by nucleophosmin: new insights from protein dissection (373 visite)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2014 Jun; 1840(6): 2050-2059.
Impact Factor: 5.353
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Roviello GN, Musumeci D, D'Alessandro C, Pedone C
* Binding ability of a thymine-functionalized oligolysine towards nucleic acids (308 visite)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2014 Feb 1; 22(3): 997-1002.
Impact Factor: 2.793
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Romanucci V, Milardi D, Campagna T, Gaglione M, Messere A, D'Urso A, Crisafi E, La Rosa C, Zarrelli A, Balzarini J, Di Fabio G
* Synthesis, biophysical characterization and anti-HIV activity of d(TG(3)AG) Quadruplexes bearing hydrophobic tails at the 5 '-end (329 visite)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2014 Feb 1; 22(3): 960-966.
Impact Factor: 2.793
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Cantisani M, Falanga A, Incoronato N, Russo L, De Simone A, Morelli G, Berisio R, Galdiero M, Galdiero S
* Conformational modifications of gB from herpes simplex virus type 1 analyzed by synthetic peptides (444 visite)
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2013 Nov 14; 56(21): 8366-8376.
Impact Factor: 5.48
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Farina B, Doti N, Pirone L, Malgieri G, Pedone E, Ruvo M, Fattorusso R
* Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy (429 visite)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Impact Factor: 3.191
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Galdiero S, Falanga A, Cantisani M, Vitiello M, Morelli G, Galdiero M
* Peptide-Lipid Interactions: Experiments and Applications (695 visite)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2013 Sep; 14(9): 18758-18789.
Impact Factor: 2.339
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Sandomenico A, Severino V, Chambery A, Focà A, Focà G, Farina C, Ruvo M
* A Comparative Structural and Bioanalytical Study of IVIG Clinical Lots (315 visite)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2013 Jul; 54(3): 983-995.
Impact Factor: 2.275
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Madonna S, Scarponi C, Doti N, Carbone T, Cavani A, Scognamiglio PL, Marasco D, Albanesi C
* Therapeutical potential of a peptide mimicking the SOCS1 kinase inhibitory region in skin immune responses (534 visite)
Eur J Immunol (ISSN: 0014-2980), 2013 Jul; 43(7): 1883-1895.
Impact Factor: 4.518
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (318 visite)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (311 visite)
Chembiochem (ISSN: 1439-4227, 1439-9422, 1439-7633), 2013 Jan 2; 14(1): 100-106.
Impact Factor: 2.92
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Causa F, Della Moglie R, Iaccino E, Mimmi S, Marasco D, Scognamiglio PL, Battista E, Palmieri C, Cosenza C, Sanguigno L, Quinto I, Scala G, Netti PA
* Evolutionary screening and adsorption behavior of engineered M13 bacteriophage and derived dodecapeptide for selective decoration of gold interfaces (367 visite)
J Colloid Interface Sci (ISSN: 0021-9797, 1095-7103electronic, 0021-9797linking), 2013 Jan 1; 389(1): 220-229.
Impact Factor: 1.646
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D'Agata R, Spoto G
* Surface plasmon resonance imaging for nucleic acid detection (318 visite)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 573-584.
Impact Factor: 3.578
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Tagliamonte M, Marasco D, Ruggiero A, de Stradis A, Tornesello ML, Totrov M, Buonaguro FM, Buonaguro L
* HIV p24 as Scaffold for Presenting Conformational HIV Env Antigens (353 visite)
Plosone (ISSN: 1932-6203, 1932-6203electronic), 2012 Sep 17; 7(8): N/D-N/D.
Impact Factor: 3.73
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D'Agata R, Spoto G
* Artificial DNA and surface plasmon resonance (493 visite)
Artificial Dna Pna & Xna (ISSN: 1949-095x), 2012 Apr; 3(2): N/D-N/D.
Impact Factor: 0
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Mercurio FA, Marasco D, Pirone L, Pedone E, Pellecchia M, Leone M
* Solution Structure of the First Sam Domain of Odin and Binding Studies with the EphA2 Receptor (415 visite)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2012 Mar 13; 51(10): 2136-2145.
Impact Factor: 3.377
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Zanoli LM, D'Agata R, Spoto G
* Functionalized gold nanoparticles for ultrasensitive DNA detection (276 visite)
Anal Bioanal Chem (ISSN: 1618-2642), 2012 Feb; 402(5): 1759-1771.
Impact Factor: 3.659
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Pilla P, Sandomenico A, Malachovsk V, Borriello A, Giordano M, Cutolo A, Ruvo M, Cusano A
* A protein-based biointerfacing route toward label-free immunoassays with long period gratings in transition mode (361 visite)
Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic, 0956-5663linking), 2012 Jan 15; 31(1): 486-491.
Impact Factor: 5.437
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Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (366 visite)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
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D'Errico G, Ercole C, Lista M, Pizzo E, Falanga A, Galdiero S, Spadaccini R, Picone D
* Enforcing the positive charge of N-termini enhances membrane interaction and antitumor activity of bovine seminal ribonuclease (421 visite)
Bba-Gen Subjects (ISSN: 0005-2736, 0006-3002, 0925-4439), 2011 Dec; 1808(12): 3007-3015.
Impact Factor: 3.99
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D'Agata R, Breveglieri G, Zanoli LM, Borgatti M, Spoto G, Gambari R
* Direct Detection of Point Mutations in Nonamplified Human Genomic DNA (636 visite)
Analytical Chemistry (ISSN: 0003-2700), 2011 Nov 15; 83(22): 8711-8717.
Impact Factor: 5.856
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Vitagliano L, Fiume G, Scognamiglio PL, Doti N, Cannavo R, Puca A, Pedone C, Scala G, Quinto I, Marasco D
* Structural and functional insights into IkappaB-alpha/HIV-1 Tat interaction (523 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Sep; 93(9): 1592-1600.
Impact Factor: 3.022
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Esposito C, Marasco D, Delogu G, Pedone E, Berisio R
* Heparin-binding hemagglutinin HBHA from Mycobacterium tuberculosis affects actin polymerisation (290 visite)
Biochem Biophys Res Commun (ISSN: 0006-291x), 2011 Jul 1; 410(2): 339-344.
Impact Factor: 2.484
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Pirone L, Correale S, De Paola I, Zaccaro L, De Simone G, Vitagliano L, Pedone E, Di Gaetano S
* Design, synthesis and characterization of a peptide able to bind proteins of the KCTD family: implications for KCTD - cullin 3 recognition (353 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2011 May; 17(5): 373-376.
Impact Factor: 1.799
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Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G
* Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions (344 visite)
Nucleic Acids Res (ISSN: 1362-4962, 0305-1048, 1362-4962electronic), 2010 Dec; 38(22): 8239-8256.
Impact Factor: 7.836
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Calvanese L, Marasco D, Doti N, Saporito A, D'Auria G, Paolillo L, Ruvo M, Falcigno L
* Structural Investigations on the Nodal-Cripto Binding: A Theoretical and Experimental Approach (412 visite)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2010 Nov; 93(11): 1011-1021.
Impact Factor: 2.572
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Palmieri C, Falcone C, Iaccino E, Tuccillo FM, Gaspari M, Trimboli F, De Laurentiis A, Luberto L, Pontoriero M, Pisano A, Vecchio E, Fierro O, Panico M, Larobina M, Gargiulo S, Costa N, Dal Piaz F, Schiavone M, Arra C, Giudice A, Palma G, Barbieri A, Quinto I, Scala G
* In vivo targeting and growth inhibition of the A20 murine B-cell lymphoma by an idiotype-specific peptide binder (495 visite)
Blood (ISSN: 0006-4971, 0006-6497, 0390-6078), 2010 Jul 15; 116(2): 226-238.
Impact Factor: 10.558
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Galdiero S, Falanga A, Vitiello M, Raiola L, Russo L, Pedone C, Isernia C, Galdiero M
* The presence of a single N-terminal histidine residue enhances the fusogenic properties of a Membranotropic peptide derived from herpes simplex virus type 1 glycoprotein (432 visite)
Jbc Papers (ISSN: 1083-351x, 0021-9258, 0021-9258linking), 2010 May 28; 285(22): 17123-17136.
Impact Factor: 5.328
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA (347 visite)
Lect Notes Electr Eng, 2010 May 15; 91LNEE: 485-488.
Impact Factor: 0
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging (324 visite)
Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2010 May 15; 25(9): 2095-2100.
Impact Factor: 5.361
Dettagli Esporta in BibTeX Esporta in EndNote
Galdiero S, Falanga A, Vitiello G, Vitiello M, Pedone C, D'Errico G, Galdiero M
* Role of membranotropic sequences from herpes simplex virus type I glycoproteins B and H in the fusion process (515 visite)
Bba-Gen Subjects (ISSN: 0006-3002, 0005-2736, 0925-4439), 2010 Mar; 1798(3): 579-591.
Impact Factor: 4.647
Dettagli Esporta in BibTeX Esporta in EndNote
D'Agata R, Breveglieri G, Zanoli L, Borgatti M, Spoto G, Gambari R
* Surface Plasmon Resonance Imaging (SPR-I), peptide nucleic acid (PNA) probes and nanoparticle-enhancement for PCR-free ultrasensitive detection of beta-thalassemia mutations in human genomic DNA (262 visite)
International Journal Of Molecular Medicine (ISSN: 1107-3756), 2010; 26: N/D-N/D.
Impact Factor: 1.814
Dettagli Esporta in BibTeX Esporta in EndNote
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S
* Viral fusion peptides induce several signal transduction pathway activations that are essential for interleukin-10 and beta-interferon production (432 visite)
Intervirology (ISSN: 1423-0100, 0300-5526), 2010; 53(6): 381-389.
Impact Factor: 1.756
Dettagli Esporta in BibTeX Esporta in EndNote
Bellia F, Calabrese V, Guarino F, Cavallaro M, Cornelius C, De Pinto V, Rizzarelli E
* Carnosinase levels in aging brain: redox state induction and cellular stress response (334 visite)
Antioxid Redox Signal (ISSN: 1557-7716, 1523-0864, 1523-0864linking), 2009 Nov; 11(11): 2759-2775.
Impact Factor: 7.581
Dettagli Esporta in BibTeX Esporta in EndNote
Sandomenico A, Monti SM, Marasco D, Dathan N, Palumbo R, Saviano M, Ruvo M
* Ige-Binding Properties And Selectivity Of Peptide Mimics Of The Fcvarepsilonri Binding Site (1605 visite)
Mol Immunol (ISSN: 0161-5890), 2009 Oct; 46(16): 3300-3309.
Impact Factor: 3.202
Dettagli Esporta in BibTeX Esporta in EndNote
Marasco D, Stilo R, Sandomenico A, Monti SM, Tizzano B, De Capua A, Varricchio E, Liguoro D, Zotti T, Formisano S, Ruvo M, Vito P
* Generation And Functional Characterization Of A Bcl10-Inhibitory Peptide That Represses Nf-Kappa B Activation (361 visite)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2009 Sep 15; 422(3): 553-561.
Impact Factor: 5.155
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, D'Agata R, Zanoli L, Spoto G
* Microfluidic networks for surface plasmon resonance imaging real-time kinetics experiments (256 visite)
Microchemical Journal (ISSN: 0026-265x), 2009 Sep; 93(1): 82-86.
Impact Factor: 2.579
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, Bush AI, D'Agata R, Rizzarelli E, Spoto G
* Enzyme solid-state support assays: a surface plasmon resonance and mass spectrometry coupled study of immobilized insulin degrading enzyme (345 visite)
Eur Biophys J (ISSN: 1432-1017, 0175-7571), 2009 Apr; 38(4): 407-414.
Impact Factor: 2.437
Dettagli Esporta in BibTeX Esporta in EndNote
Ciaccio C, Tundo GR, Grasso G, Spoto G, Marasco D, Ruvo M, Gioia M, Rizzarelli E, Coletta M
* Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity (424 visite)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 Feb 6; 385(5): 1556-1567.
Impact Factor: 3.871
Dettagli Esporta in BibTeX Esporta in EndNote
Sandomenico A, Marasco D, Monti SM, Saviano M, Pedone C, Benedetti E, Ruvo M
* Peptides binding the type E immunoglobulins (400 visite)
Adv Exp Med Biol (ISSN: 0065-2598, 0065-2598print), 2009; 611: 573-574.
Impact Factor: 2.02
Dettagli Esporta in BibTeX Esporta in EndNote
Ruvo M, Sandomenico A, Marasco D, Monti SM, Tizzano B, De Capua A, Pedone C, Saporito A, Vitale RM, Formisano S, Stilo R, Liguoro D
* Self-association regions in the CARD of Bcl-10 (341 visite)
Adv Exp Med Biol (ISSN: 0065-2598, 0065-2598print), 2009; 611: 569-570.
Impact Factor: 2.02
Dettagli Esporta in BibTeX Esporta in EndNote
Gioia M, Monaco S, Van Den Steen PE, Sbardella D, Grasso G, Marini S, Overall CM, Opdenakker G, Coletta M
* The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase (378 visite)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Impact Factor: 3.871
Dettagli Esporta in BibTeX Esporta in EndNote
Viparelli F, Cassese A, Doti N, Paturzo F, Marasco D, Dathan NA, Monti SM, Basile G, Ungaro P, Sabatella M, Miele C, Teperino R, Consiglio E, Pedone C, Beguinot F, Formisano P, Ruvo M
* Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (389 visite)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Sep 1; 283(31): 21769-21778.
Impact Factor: 5.52
Dettagli Esporta in BibTeX Esporta in EndNote
D'Agata R, Corradini R, Grasso G, Marchelli R, Spoto G
* Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging (366 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2008 Sep 1; 9(13): 2067-2070.
Impact Factor: 3.322
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, Rizzarelli E, Spoto G
* How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin (323 visite)
Bba-Gen Subjects (ISSN: 0006-3002, 1570-9639, 0925-4439), 2008 Jul; 1784(7-8): 1122-1126.
Impact Factor: 2.233
Dettagli Esporta in BibTeX Esporta in EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (408 visite)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
Dettagli Esporta in BibTeX Esporta in EndNote
Cigliano L, Maresca B, Salvatore A, Nino M, Monfrecola G, Ayala F, Carlucci A, Pugliese RC, Pedone C, Abrescia P
* Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity (339 visite)
Journal Of The European Academy Of Dermatology And Venereology (ISSN: 1468-3083, 0926-9959), 2008 Apr; 22(4): 417-425.
Impact Factor: 2.276
Dettagli Esporta in BibTeX Esporta in EndNote
Gallotta G, Palmieri V, Piedimonte V, Rendina D, De Bonis S, Russo V, Celentano A, Di Minno M, Postiglione A, Di Minno G
* Increased troponin I predicts in-hospital occurrence of hemodynamic instability in patients with sub-massive or non-massive pulmonary embolism independent to clinical, echocardiographic and laboratory information (254 visite)
Int J Cardiol (ISSN: 0167-5273, 1874-1754electronic, 0167-5273linking), 2008 Mar 14; 124(3): 351-357.
Impact Factor: 3.121
Dettagli Esporta in BibTeX Esporta in EndNote
Migliore T, Parrella LS, Caputi A, Silvestri N, Romano R, Pace L, Imbriaco M, Losi MA, Betocchi S
* Pathogenesis of hypertrophic cardiomyopathy. Impact of growth factors on left ventricular anatomy (332 visite)
Minerva Cardioangiol (ISSN: 0026-4725), 2008 Feb; 56(1): 13-20.
Impact Factor: 0.481
Dettagli Esporta in BibTeX Esporta in EndNote
Rossi M, Ruvo M, Marasco D, Colombo M, Cassani G, Verdoliva A
* Anti-allergic properties of a new all-D synthetic immunoglobulin-binding peptide (402 visite)
Mol Immunol (ISSN: 0161-5890), 2008 Jan; 45(1): 226-234.
Impact Factor: 3.555
Dettagli Esporta in BibTeX Esporta in EndNote
Zanoli L, Dagata R, Spoto G
* Surface plasmon-based optical detection of DNA peptide nucleic acids (244 visite)
Minerva Biotecnol (ISSN: 1120-4826), 2008; 20(4): 165-174.
Impact Factor: 0.1
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111) (306 visite)
Journal Of The American Society For Mass Spectrometry (ISSN: 1044-0305), 2007 May; 18(5): 961-969.
Impact Factor: 3.664
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, Fragai M, Rizzarelli E, Spoto G, Yeo KJ
* In situ AP/MALDI-MS characterization of anchored matrix metalloproteinases (381 visite)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2006 Dec; 41(12): 1561-1569.
Impact Factor: 2.945
Dettagli Esporta in BibTeX Esporta in EndNote
D'Agata R, Grasso G, Iacono G, Spoto G, Vecchio G
* Lectin recognition of a new SOD mimic bioconjugate studied with surface plasmon resonance imaging (273 visite)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2006 Feb 21; 4(4): 610-612.
Impact Factor: 2.874
Dettagli Esporta in BibTeX Esporta in EndNote
Grasso G, D'Agata R, Rizzarelli E, Spoto G, D'Andrea LD, Pedone C, Picardi A, Romanelli A, Fragai M, Yeo KJ
* Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS (478 visite)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2005 Dec; 40(12): 1565-1571.
Impact Factor: 3.574
Dettagli Esporta in BibTeX Esporta in EndNote
Verdoliva A, Marasco D, De Capua A, Saporito A, Bellofiore P, Manfredi V, Fattorusso R, Pedone C, Ruvo M
* A new ligand for immunoglobulin G subdomains by screening of a synthetic peptide library (433 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2005 Jul; 6(7): 1242-1253.
Impact Factor: 3.94
Dettagli Esporta in BibTeX Esporta in EndNote
Arena G, Contino A, D'Agata R, Sgarlata C, Spoto G
* Ordered anchored cavities at work: A new and rapid SPR-based method for the detection of trace amounts of Cs+ (387 visite)
New J Chem (ISSN: 1144-0546), 2005; 29(11): 1393-1395.
Impact Factor: 2.574
Dettagli Esporta in BibTeX Esporta in EndNote
Rossi M, Manfredi V, Ruvo M, Fassina G, Verdoliva A
* Sequence-simplification and chimeric assembly: New models of peptide antigen modification (358 visite)
Mol Immunol (ISSN: 0161-5890), 2002 Nov; 39(7-8): 443-451.
Impact Factor: 2.414
Dettagli Esporta in BibTeX Esporta in EndNote
De Falco S, Ruvo M, Verdoliva A, Scarallo A, Raimondo D, Raucci A, Fassina G
* N-terminal myristylation of HBV preS1 domain enhances receptor recognition (391 visite)
J Pept Res (ISSN: 1397-002x, 1397-002xlinking), 2001 May; 57(5): 390-400.
Impact Factor: 1.522
Dettagli Esporta in BibTeX Esporta in EndNote
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S
* Fusion in Coq (424 visite)
Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596.
Impact Factor: 0.415
Dettagli Esporta in BibTeX Esporta in EndNote
Monti SM, Fogliano V, Randazzo G, Peluso G, Logrieco A, Ritieni A
Polyclonal antibodies against fusaproliferin (276 visite)
Can J Microbiol (ISSN: 0008-4166), 1999 Jan; 45(1): 45-50.
Impact Factor: 1.072
Dettagli Esporta in BibTeX Esporta in EndNote
75 Records (72 escludendo Abstract e Conferenze).
Impact factor totale: 240.622 (235.078 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 241.26 (235.197 escludendo Abstract e Conferenze).
Impact factor totale: 240.622 (235.078 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 241.26 (235.197 escludendo Abstract e Conferenze).
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