Self-assembled or mixed peptide amphiphile micelles from herpes simplex virus glycoproteins as potential immunomodulatory treatment (921 views)
Int J Nanomed (ISSN: 1178-2013, 1176-9114, 1176-9114linking), 2014 May 7; 9(1): 2137-2148.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 4.383, 5-year impact factor: 4.742
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84900384692&partnerID=40&md5=f49ec43f6f86a8d0673db90c91d6bee8
Keywords: Epitopes, Hsv, Self-Adjuvant Vaccine, Glycoprotein B, Herpes Simplex Vaccine, Interleukin 23, Macrophage Inflammatory Protein 2, Tumor Necrosis Factor Alpha, Cytokine, Simplexvirus, Herpes Simplex Virus Type 1, Immunologic Factor, Micelle, Peptide, Surfactant, Virus Envelope Protein, Amino Terminal Sequence, Animal Cell, Article, Controlled Study, Cytokine Release, Drug Formulation, Drug Mixture, Human, Human Cell, Hydrophobicity, Immunomodulation, Micellization, Mouse, Nonhuman, Peptide Analysis, Solid, Vaccine Production, Zeta Potential, Chemistry, Drug Effects, Feasibility Study, Synthesis, U937 Cell Line, Feasibility Studies, Surface-Active Agents, Viral Envelope Proteins, Cytokines Immunology, Immunologic Factors Chemical Synthesis Pharmacology, Macrophages Drug Effects Immunology, Peptides Chemical Synthesis Pharmacology, Surface-Active Agents Chemical Synthesis Pharmacology, Viral Envelope Proteins Chemistry,
Affiliations:
*** IBB - CNR ***
Department of Pharmacy, Interuniversitary Centre for Research on Bioactive peptides, CIRPeB, University of Naples Federico II, Institute of Biostructures and Bioimaging IBB-CNR, Naples, Italy
Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy
Department of Clinical Pathology and Transfusion Medicine, University Hospital Ruggi d'Aragona, Salerno, Italy
References:
Not available.
Int J Nanomed (ISSN: 1178-2013, 1176-9114, 1176-9114linking), 2014 May 7; 9(1): 2137-2148.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 4.383, 5-year impact factor: 4.742
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84900384692&partnerID=40&md5=f49ec43f6f86a8d0673db90c91d6bee8
Keywords: Epitopes, Hsv, Self-Adjuvant Vaccine, Glycoprotein B, Herpes Simplex Vaccine, Interleukin 23, Macrophage Inflammatory Protein 2, Tumor Necrosis Factor Alpha, Cytokine, Simplexvirus, Herpes Simplex Virus Type 1, Immunologic Factor, Micelle, Peptide, Surfactant, Virus Envelope Protein, Amino Terminal Sequence, Animal Cell, Article, Controlled Study, Cytokine Release, Drug Formulation, Drug Mixture, Human, Human Cell, Hydrophobicity, Immunomodulation, Micellization, Mouse, Nonhuman, Peptide Analysis, Solid, Vaccine Production, Zeta Potential, Chemistry, Drug Effects, Feasibility Study, Synthesis, U937 Cell Line, Feasibility Studies, Surface-Active Agents, Viral Envelope Proteins, Cytokines Immunology, Immunologic Factors Chemical Synthesis Pharmacology, Macrophages Drug Effects Immunology, Peptides Chemical Synthesis Pharmacology, Surface-Active Agents Chemical Synthesis Pharmacology, Viral Envelope Proteins Chemistry,
Affiliations:
*** IBB - CNR ***
Department of Pharmacy, Interuniversitary Centre for Research on Bioactive peptides, CIRPeB, University of Naples Federico II, Institute of Biostructures and Bioimaging IBB-CNR, Naples, Italy
Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy
Department of Clinical Pathology and Transfusion Medicine, University Hospital Ruggi d'Aragona, Salerno, Italy
References:
Not available.
Self-assembled or mixed peptide amphiphile micelles from herpes simplex virus glycoproteins as potential immunomodulatory treatment
The use of micelle aggregates formed from peptide amphiphiles (PAs) as potential synthetic self-adjuvant vaccines to treat Herpes simplex virus (HSV) infection are reported here. The PAs were based on epitopes gB409-505 and gD301-309, selected from HSV envelope glycoprotein B (gB) and glycoprotein D (gD), that had their N-terminus modified with hydrophobic moieties containing two C18 hydrocarbon chains. Pure and mixed micelles of gB and/or gD peptide epitopes were easily prepared after starting with the synthesis of corresponding PAs by solid phase methods. Structural characterization of the aggregates confirmed that they were sufficiently stable and compatible with in vivo use: critical micelle concentration values around 4.0 · 10-7 mol · Kg-1; hydrodynamic radii (RH) between 50-80 nm, and a zeta potential (?) around -40 mV were found for all aggregates. The in vitro results indicate that both peptide epitopes and micelles, at 10 µM, triggered U937 and RAW 264.7 cells to release appreciable levels of cytokines. In particular, interleukin (IL)-23-, IL-6-, IL-8- or macrophage inflammatory protein (MIP)-2-, and tumor necrosis factor (TNF)-a-release increased considerably when cells were treated with the gB-micelles or gD-micelles compared with the production of the same cytokines when the stimulus was the single gB or gD peptide. © 2014 Accardo et al.
The use of micelle aggregates formed from peptide amphiphiles (PAs) as potential synthetic self-adjuvant vaccines to treat Herpes simplex virus (HSV) infection are reported here. The PAs were based on epitopes gB409-505 and gD301-309, selected from HSV envelope glycoprotein B (gB) and glycoprotein D (gD), that had their N-terminus modified with hydrophobic moieties containing two C18 hydrocarbon chains. Pure and mixed micelles of gB and/or gD peptide epitopes were easily prepared after starting with the synthesis of corresponding PAs by solid phase methods. Structural characterization of the aggregates confirmed that they were sufficiently stable and compatible with in vivo use: critical micelle concentration values around 4.0 · 10-7 mol · Kg-1; hydrodynamic radii (RH) between 50-80 nm, and a zeta potential (?) around -40 mV were found for all aggregates. The in vitro results indicate that both peptide epitopes and micelles, at 10 µM, triggered U937 and RAW 264.7 cells to release appreciable levels of cytokines. In particular, interleukin (IL)-23-, IL-6-, IL-8- or macrophage inflammatory protein (MIP)-2-, and tumor necrosis factor (TNF)-a-release increased considerably when cells were treated with the gB-micelles or gD-micelles compared with the production of the same cytokines when the stimulus was the single gB or gD peptide. © 2014 Accardo et al.
Self-assembled or mixed peptide amphiphile micelles from herpes simplex virus glycoproteins as potential immunomodulatory treatment
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Self-assembled or mixed peptide amphiphile micelles from herpes simplex virus glycoproteins as potential immunomodulatory treatment
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Falanga A, Tarallo R, Vitiello G, Vitiello M, Perillo E, Cantisani M, D'Errico G, Galdiero M, Galdiero S
* Biophysical Characterization and Membrane Interaction of the Two Fusion Loops of Glycoprotein B from Herpes Simplex Type I Virus (1217 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 Feb 23; 7(2): N/D-N/D.
Impact Factor: 3.73
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Galdiero S, Vitiello M, Finamore E, Mansi R, Galdiero M, Morelli G, Tesauro D
* Activation of monocytic cells by immunostimulatory lipids conjugated to peptide antigens (937 views)
Molecular Biosystems (ISSN: 1742-206x), 2012; 8(12): 3166-3177.
Impact Factor: 3.35
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Vitiello G, Falanga A, Galdiero M, Marsh D, Galdiero S, D'Errico G
* Lipid composition modulates the interaction of peptides deriving from herpes simplex virus type I glycoproteins B and H with biomembranes (1436 views)
Bba-Gen Subjects (ISSN: 0005-2736, 0006-3002, 0925-4439), 2011 Oct; 1808(10): 2517-2526.
Impact Factor: 3.99
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Galdiero S, Vitiello M, D'Isanto M, Falanga A, Cantisani M, Browne H, Pedone C, Galdiero M
* The identification and characterization of fusogenic domains in herpes virus glycoprotein B molecules (1108 views)
Chembiochem (ISSN: 1439-7633, 1439-4227, 1439-7633electronic), 2008 Mar 25; 9(5): 758-767.
Impact Factor: 3.322
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Galdiero S, Vitiello M, D'Isanto M, Falanga A, Collins C, Raieta K, Pedone C, Browne H, Galdiero M
* Analysis of synthetic peptides from heptad-repeat domains of herpes simplex virus type 1 glycoproteins H and (993 views)
Journal Of General Virology (ISSN: 0022-1317), 2006 May; 87: 1085-1097.
Impact Factor: 3.11
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Galdiero S, Falanga A, Vitiello M, Browne H, Pedone C, Galdiero M
* Fusogenic domains in herpes simplex virus type 1 glycoprotein (852 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Sep 5; 280(31): 28632-28643.
Impact Factor: 5.854
View Export to BibTeX Export to EndNote
* Biophysical Characterization and Membrane Interaction of the Two Fusion Loops of Glycoprotein B from Herpes Simplex Type I Virus (1217 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2012 Feb 23; 7(2): N/D-N/D.
Impact Factor: 3.73
View Export to BibTeX Export to EndNote
Galdiero S, Vitiello M, Finamore E, Mansi R, Galdiero M, Morelli G, Tesauro D
* Activation of monocytic cells by immunostimulatory lipids conjugated to peptide antigens (937 views)
Molecular Biosystems (ISSN: 1742-206x), 2012; 8(12): 3166-3177.
Impact Factor: 3.35
View Export to BibTeX Export to EndNote
Vitiello G, Falanga A, Galdiero M, Marsh D, Galdiero S, D'Errico G
* Lipid composition modulates the interaction of peptides deriving from herpes simplex virus type I glycoproteins B and H with biomembranes (1436 views)
Bba-Gen Subjects (ISSN: 0005-2736, 0006-3002, 0925-4439), 2011 Oct; 1808(10): 2517-2526.
Impact Factor: 3.99
View Export to BibTeX Export to EndNote
Galdiero S, Vitiello M, D'Isanto M, Falanga A, Cantisani M, Browne H, Pedone C, Galdiero M
* The identification and characterization of fusogenic domains in herpes virus glycoprotein B molecules (1108 views)
Chembiochem (ISSN: 1439-7633, 1439-4227, 1439-7633electronic), 2008 Mar 25; 9(5): 758-767.
Impact Factor: 3.322
View Export to BibTeX Export to EndNote
Galdiero S, Vitiello M, D'Isanto M, Falanga A, Collins C, Raieta K, Pedone C, Browne H, Galdiero M
* Analysis of synthetic peptides from heptad-repeat domains of herpes simplex virus type 1 glycoproteins H and (993 views)
Journal Of General Virology (ISSN: 0022-1317), 2006 May; 87: 1085-1097.
Impact Factor: 3.11
View Export to BibTeX Export to EndNote
Galdiero S, Falanga A, Vitiello M, Browne H, Pedone C, Galdiero M
* Fusogenic domains in herpes simplex virus type 1 glycoprotein (852 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Sep 5; 280(31): 28632-28643.
Impact Factor: 5.854
View Export to BibTeX Export to EndNote
6 Records (5 excluding Abstracts).
Total impact factor: 23.356 (19.626 excluding Abstracts).
Total 5 year impact factor: 23.978 (19.734 excluding Abstracts).
Total impact factor: 23.356 (19.626 excluding Abstracts).
Total 5 year impact factor: 23.978 (19.734 excluding Abstracts).
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