Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization(748 views) Hayafune M, Berisio R, Marchetti R, Silipo A, Kayama M, Desaki Y, Arima S, Squeglia F, Ruggiero A, Tokuyasu K, Molinaro A, Kaku H, Shibuya N
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2014 Jan 21; 111(3): E404-E404.
Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan
Institute of Biostructures and Bioimaging, National Research Council, I-80134 Naples, Italy
Department of Chemical Sciences, Universita di Napoli Federico II, I-80126 Naples, Italy
Food Resource Division, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan
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Assadi-Porter, F. M., Direct NMR detection of the binding of functional ligands to the human sweet receptor, a heterodimeric family 3 GPCR (2008) J Am Chem Soc, 130 (23), pp. 7212-7213
Szczepina, M. G., Bleile, D. W., Pinto, B. M., Investigation of the binding of a carbohydrate-mimetic peptide to its complementary anticarbohydrate antibody by STD-NMR spectroscopy and molecular-dynamics simulations (2011) Chemistry, 17 (41), pp. 11446-11455
Enr quez-Navas, P. M., Marradi, M., Padro, D., Angulo, J., Penad s, S., A solution NMR study of the interactions of oligomannosides and the anti-HIV-1 2G12 antibody reveals distinct binding modes for branched ligands (2011) Chemistry, 17 (5), pp. 1547-1560
Johnson, M. A., Pinto, B. M., NMR spectroscopic and molecular modeling studies of protein-carbohydrate and protein-peptide interactions (2004) Carbohydr Res, 339 (5), pp. 907-928
Liu, T. T., Chitin-induced dimerization activates a plant immune receptor (2012) Science, 336 (6085), pp. 1160-1164
Hubbard, S. R., Till, J. H., Protein tyrosine kinase structure and function (2000) Annu Rev Biochem, 69, pp. 373-398
Heldin, C. H., Dimerization of cell surface receptors in signal transduction (1995) Cell, 80 (2), pp. 213-223
Curtis, M. D., Grossniklaus, U., A gateway cloning vector set for high-throughput functional analysis of genes in planta (2003) Plant Physiol, 133 (2), pp. 462-469
States, D. J., Haberkorn, R. A., Ruben, D. J., A two-dimensional nuclear overhauser experiment with pure absorption phase in 4 quadrants (1982) J Magn Reson, 48 (2), pp. 286-292
Asensio, J. L., Jimenez-Barbero, J., The use of the AMBER force field in conformational analysis of carbohydrate molecules: Determination of the solution conformation of methyl -lactoside by NMR spectroscopy, assisted by molecular mechanics and dynamics calculations (1995) Biopolymers, 35 (1), pp. 55-73
Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization
Perception of microbe-associated molecular patterns (MAMPs) through pattern recognition receptors (PRRs) triggers various defense responses in plants. This MAMP-triggered immunity plays a major role in the plant resistance against various pathogens. To clarify the molecular basis of the specific recognition of chitin oligosaccharides by the rice PRR, CEBiP (chitin-elicitor binding protein), as well as the formation and activation of the receptor complex, biochemical, NMR spectroscopic, and computational studies were performed. Deletion and domain-swapping experiments showed that the central lysine motif in the ectodomain of CEBiP is essential for the binding of chitin oligosaccharides. Epitope mapping by NMR spectroscopy indicated the preferential binding of longer-chain chitin oligosaccharides, such as heptamer-octamer, to CEBiP, and also the importance of N-acetyl groups for the binding. Molecular modeling/docking studies clarified the molecular interaction between CEBiP and chitin oligosaccharides and indicated the importance of Ile (122) in the central lysine motif region for ligand binding, a notion supported by site-directed mutagenesis. Based on these results, it was indicated that two CEBiP molecules simultaneously bind to one chitin oligosaccharide from the opposite side, resulting in the dimerization of CEBiP. The model was further supported by the observations that the addition of (GlcNAc) (8) induced dimerization of the ectodomain of CEBiP in vitro, and the dimerization and (GlcNAc) (8) -induced reactive oxygen generation were also inhibited by a unique oligosaccharide, (GlcN beta 1, 4GlcNAc) (4), which is supposed to have N-acetyl groups only on one side of the molecule. Based on these observations, we proposed a hypothetical model for the ligand-induced activation of a receptor complex, involving both CEBiP and Oryza sativa chitin-elicitor receptor kinase-1
Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization
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