Dissecting the contribution of thrombin exosite I in the recognition of thrombin binding aptamer (465 views)
Febs Journal (ISSN: 1742-464x), 2013 Dec; 280(24): 6581-6588.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.986, 5-year impact factor: 3.673
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84889679159&partnerID=40&md5=1f45d2202a9a3ad23e2a5852b16dc0c8
Keywords: Biosensors, G-Quadruplex, Human Thrombin, Protein-Nucleic Acid Complex, Thrombin Binding Aptamer, Thrombosis, Guanine Quadruplex, Thrombin Binding Aptamer Delta T12, Thrombin Exosite I, Unclassified Drug, Article, Complex Formation, Crystallography, Molecular Mechanics, Molecular Recognition, Priority Journal, Protein Binding, Protein Folding, Protein Function, Protein Interaction, Protein Modification, Structure Analysis, Nucleotide, Circular Dichroism, Crystallization, Mutation,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples, Italy
Institute of Biostructures and Bioimaging, CNR, Naples, Italy
Medical Proteomics Laboratory, Institute of Medical Science, University of Tokyo, Japan
Department of Bioengineering, Graduate School of Engineering, University of Tokyo, Japan
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan
Interuniversity Consortium of Structural and Systems Biology, Rome, Italy
References:
Janin, J., Rodier, F., Chakrabarti, P., Bahadur, R.P., Macromolecular recognition in the Protein Data Bank (2007) Acta Crystallogr D, 63, pp. 1-
Hong, H., Goel, S., Zhang, Y., Cai, W., Molecular imaging with nucleic acid aptamers (2011) Curr Med Chem, 18, pp. 4195-4205
Keefe, A.D., Pai, S., Ellington, A., Aptamers as therapeutics (2010) Nat Rev Drug Discov, 9, pp. 537-550
Hermann, T., Patel, D.J., Adaptive recognition by nucleic acid aptamers (2000) Science, 287, pp. 820-825
Tan, W., Wang, H., Chen, Y., Zhang, X., Zhu, H., Yang, C., Yang, R., Liu, C., Molecular aptamers for drug delivery (2011) Trends Biotechnol, 29, pp. 634-640
Musumeci, D., Montesarchio, D., Polyvalent nucleic acid aptamers and modulation of their activity: A focus on the thrombin binding aptamer (2012) Pharmacol Ther, 136, pp. 202-215
Dhar, S., Gu, F.X., Langer, R., Farokhzad, O.C., Lippard, S.J., Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt(IV) prodrug-PLGA-PEG nanoparticles (2008) Proc Natl Acad Sci USA, 105, pp. 17356-17361
Bunka, D.H., Platonova, O., Stockley, P.G., Development of aptamer therapeutics (2010) Curr Opin Pharmacol, 10, pp. 557-562
Bompiani, K.M., Woodruff, R.S., Becker, R.C., Nimjee, S.M., Sullenger, B.A., Antidote control of aptamer therapeutics: The road to a safer class of drug agents (2012) Curr Pharm Biotechnol, 13, pp. 1924-1934
Avino, A., Fabrega, C., Tintore, M., Eritja, R., Thrombin binding aptamer, more than a simple aptamer: Chemically modified derivatives and biomedical applications (2012) Curr Pharm des, 18, pp. 2036-2047
Huntington, J.A., Molecular recognition mechanisms of thrombin (2005) J Thromb Haemost, 3, pp. 1861-1872
Fenton II, J.W., Thrombin specificity (1981) Ann N y Acad Sci, 370, pp. 468-495
Adams, T.E., Huntington, J.A., Thrombin-cofactor interactions: Structural insights into regulatory mechanisms (2006) Arterioscler Thromb Vasc Biol, 26, pp. 1738-1745
Welsby, I.J., Monroe, D.M., Lawson, J.H., Hoffmann, M., Recombinant activated factor VII and the anaesthetist (2005) Anaesthesia, 60, pp. 1203-1212
Bock, L.C., Griffin, L.C., Latham, J.A., Vermaas, E.H., Toole, J.J., Selection of single-stranded DNA molecules that bind and inhibit human thrombin (1992) Nature, 355, pp. 564-566
Russo Krauss, I., Pica, A., Merlino, A., Mazzarella, L., Sica, F., Duplex-quadruplex motifs in a peculiar structural organization cooperatively contribute to thrombin binding of a DNA aptamer (2013) Acta Crystallogr D, 69. , doi: 10.1107/S0907444913022269
Russo Krauss, I., Merlino, A., Randazzo, A., Novellino, E., Mazzarella, L., Sica, F., High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity (2012) Nucleic Acids Res, 40, pp. 8119-8128
MacAya, R.F., Schultze, P., Smith, F.W., Roe, J.A., Feigon, J., Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution (1993) Proc Natl Acad Sci USA, 90, pp. 3745-3749
Burge, S., Parkinson, G.N., Hazel, P., Todd, A.K., Neidle, S., Quadruplex DNA: Sequence, topology and structure (2006) Nucleic Acids Res, 34, pp. 5402-5415
Borbone, N., Bucci, M., Oliviero, G., Morelli, E., Amato, J., D'Atri, V., D'Errico, S., Piccialli, G., Investigating the role of T7 and T12 residues on the biological properties of thrombin-binding aptamer: Enhancement of anticoagulant activity by a single nucleobase modification (2012) J Med Chem, 55, pp. 10716-10728
Sagi, J., G-quadruplexes incorporating modified constituents: A review (2013) J Biomol Struct Dyn, , in press. doi: 10.1080/07391102.2013.775074
Bunka, D.H., Stockley, P.G., Aptamers come of age - At last (2006) Nat Rev Microbiol, 4, pp. 588-596
Nagatoishi, S., Sugimoto, N., Interaction of water with the G-quadruplex loop contributes to the binding energy of G-quadruplex to protein (2012) Mol BioSyst, 8, pp. 2766-2770
Figueiredo, A.C., Clement, C.C., Zakia, S., Gingold, J., Philipp, M., Pereira, P.J., Rational design and characterization of D-Phe-Pro-D-Arg-derived direct thrombin inhibitors (2012) PLoS ONE, 7, pp. e34354
Lima, L.M., Becker, C.F., Giesel, G.M., Marques, A.F., Cargnelutti, M.T., De Oliveira Neto, M., Monteiro, R.Q., Polikarpov, I., Structural and thermodynamic analysis of thrombin:suramin interaction in solution and crystal phases (2009) Biochim Biophys Acta, 1794, pp. 873-881
Davis, J.T., G-quartets 40 years later: From 5′-GMP to molecular biology and supramolecular chemistry (2004) Angew Chem Int Ed Engl, 43, pp. 668-698
Bonifacio, L., Church, F.C., Jarstfer, M.B., Effect of locked-nucleic acid on a biologically active G-quadruplex. A structure-activity relationship of the thrombin aptamer (2008) Int J Mol Sci, 9, pp. 422-433
Heckel, A., Mayer, G., Light regulation of aptamer activity: An anti-thrombin aptamer with caged thymidine nucleobases (2005) J Am Chem Soc, 127, pp. 822-823
Nagatoishi, S., Isono, N., Tsumoto, K., Sugimoto, N., Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding (2011) Biochimie, 93, pp. 1231-1238
Padmanabhan, K., Tulinsky, A., An ambiguous structure of a DNA 15-mer thrombin complex (1996) Acta Crystallogr D, 52, pp. 272-282
Otwinowski, Z., Minor, W., Processing of X-ray diffraction data collected in oscillation mode (1997) Methods in Enzymology, pp. 307-326. , In (Carter Jr.C.W., ed.), Academic Press, New York
McCoy, A.J., Grosse-Kunstleve, R.W., Adams, P.D., Winn, M.D., Storoni, L.C., Read, R.J., Phaser crystallographic software (2007) J Appl Crystallogr, 40, pp. 658-674
Brunger, A.T., Adams, P.D., Clore, G.M., Delano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Pannu, N.S., Crystallography and NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallogr D, 54, pp. 905-921
Vagin, A.A., Steiner, R.A., Lebedev, A.A., Potterton, L., McNicholas, S., Long, F., Murshudov, G.N., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr D, 60, pp. 2184-2195
Emsley, P., Lohkamp, B., Scott, W.G., Cowtan, K., Features and development of Coot (2010) Acta Crystallogr D, 66, pp. 486-501
Laskowski, R.A., Moss, D.S., Thornton, J.M., Main-chain bond lengths and bond angles in protein structures (1993) J Mol Biol, 231, pp. 1049-1067
Schrodinger, L., (2010) The PyMOL Molecular Graphics System, Version 1.3, , Schrödinger, LLC
Keefe, A. D., Pai, S., Ellington, A., Aptamers as therapeutics (2010) Nat Rev Drug Discov, 9, pp. 537-550
Dhar, S., Gu, F. X., Langer, R., Farokhzad, O. C., Lippard, S. J., Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt (IV) prodrug-PLGA-PEG nanoparticles (2008) Proc Natl Acad Sci USA, 105, pp. 17356-17361
Bunka, D. H., Platonova, O., Stockley, P. G., Development of aptamer therapeutics (2010) Curr Opin Pharmacol, 10, pp. 557-562
Bompiani, K. M., Woodruff, R. S., Becker, R. C., Nimjee, S. M., Sullenger, B. A., Antidote control of aptamer therapeutics: The road to a safer class of drug agents (2012) Curr Pharm Biotechnol, 13, pp. 1924-1934
Huntington, J. A., Molecular recognition mechanisms of thrombin (2005) J Thromb Haemost, 3, pp. 1861-1872
Fenton II, J. W., Thrombin specificity (1981) Ann N y Acad Sci, 370, pp. 468-495
Adams, T. E., Huntington, J. A., Thrombin-cofactor interactions: Structural insights into regulatory mechanisms (2006) Arterioscler Thromb Vasc Biol, 26, pp. 1738-1745
Welsby, I. J., Monroe, D. M., Lawson, J. H., Hoffmann, M., Recombinant activated factor VII and the anaesthetist (2005) Anaesthesia, 60, pp. 1203-1212
Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H., Toole, J. J., Selection of single-stranded DNA molecules that bind and inhibit human thrombin (1992) Nature, 355, pp. 564-566
MacAya, R. F., Schultze, P., Smith, F. W., Roe, J. A., Feigon, J., Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution (1993) Proc Natl Acad Sci USA, 90, pp. 3745-3749
Bunka, D. H., Stockley, P. G., Aptamers come of age - At last (2006) Nat Rev Microbiol, 4, pp. 588-596
Figueiredo, A. C., Clement, C. C., Zakia, S., Gingold, J., Philipp, M., Pereira, P. J., Rational design and characterization of D-Phe-Pro-D-Arg-derived direct thrombin inhibitors (2012) PLoS ONE, 7, pp. e34354
Lima, L. M., Becker, C. F., Giesel, G. M., Marques, A. F., Cargnelutti, M. T., De Oliveira Neto, M., Monteiro, R. Q., Polikarpov, I., Structural and thermodynamic analysis of thrombin: suramin interaction in solution and crystal phases (2009) Biochim Biophys Acta, 1794, pp. 873-881
Davis, J. T., G-quartets 40 years later: From 5 -GMP to molecular biology and supramolecular chemistry (2004) Angew Chem Int Ed Engl, 43, pp. 668-698
McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., Read, R. J., Phaser crystallographic software (2007) J Appl Crystallogr, 40, pp. 658-674
Brunger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Pannu, N. S., Crystallography and NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallogr D, 54, pp. 905-921
Vagin, A. A., Steiner, R. A., Lebedev, A. A., Potterton, L., McNicholas, S., Long, F., Murshudov, G. N., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr D, 60, pp. 2184-2195
Laskowski, R. A., Moss, D. S., Thornton, J. M., Main-chain bond lengths and bond angles in protein structures (1993) J Mol Biol, 231, pp. 1049-1067
Febs Journal (ISSN: 1742-464x), 2013 Dec; 280(24): 6581-6588.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.986, 5-year impact factor: 3.673
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84889679159&partnerID=40&md5=1f45d2202a9a3ad23e2a5852b16dc0c8
Keywords: Biosensors, G-Quadruplex, Human Thrombin, Protein-Nucleic Acid Complex, Thrombin Binding Aptamer, Thrombosis, Guanine Quadruplex, Thrombin Binding Aptamer Delta T12, Thrombin Exosite I, Unclassified Drug, Article, Complex Formation, Crystallography, Molecular Mechanics, Molecular Recognition, Priority Journal, Protein Binding, Protein Folding, Protein Function, Protein Interaction, Protein Modification, Structure Analysis, Nucleotide, Circular Dichroism, Crystallization, Mutation,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples, Italy
Institute of Biostructures and Bioimaging, CNR, Naples, Italy
Medical Proteomics Laboratory, Institute of Medical Science, University of Tokyo, Japan
Department of Bioengineering, Graduate School of Engineering, University of Tokyo, Japan
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan
Interuniversity Consortium of Structural and Systems Biology, Rome, Italy
References:
Janin, J., Rodier, F., Chakrabarti, P., Bahadur, R.P., Macromolecular recognition in the Protein Data Bank (2007) Acta Crystallogr D, 63, pp. 1-
Hong, H., Goel, S., Zhang, Y., Cai, W., Molecular imaging with nucleic acid aptamers (2011) Curr Med Chem, 18, pp. 4195-4205
Keefe, A.D., Pai, S., Ellington, A., Aptamers as therapeutics (2010) Nat Rev Drug Discov, 9, pp. 537-550
Hermann, T., Patel, D.J., Adaptive recognition by nucleic acid aptamers (2000) Science, 287, pp. 820-825
Tan, W., Wang, H., Chen, Y., Zhang, X., Zhu, H., Yang, C., Yang, R., Liu, C., Molecular aptamers for drug delivery (2011) Trends Biotechnol, 29, pp. 634-640
Musumeci, D., Montesarchio, D., Polyvalent nucleic acid aptamers and modulation of their activity: A focus on the thrombin binding aptamer (2012) Pharmacol Ther, 136, pp. 202-215
Dhar, S., Gu, F.X., Langer, R., Farokhzad, O.C., Lippard, S.J., Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt(IV) prodrug-PLGA-PEG nanoparticles (2008) Proc Natl Acad Sci USA, 105, pp. 17356-17361
Bunka, D.H., Platonova, O., Stockley, P.G., Development of aptamer therapeutics (2010) Curr Opin Pharmacol, 10, pp. 557-562
Bompiani, K.M., Woodruff, R.S., Becker, R.C., Nimjee, S.M., Sullenger, B.A., Antidote control of aptamer therapeutics: The road to a safer class of drug agents (2012) Curr Pharm Biotechnol, 13, pp. 1924-1934
Avino, A., Fabrega, C., Tintore, M., Eritja, R., Thrombin binding aptamer, more than a simple aptamer: Chemically modified derivatives and biomedical applications (2012) Curr Pharm des, 18, pp. 2036-2047
Huntington, J.A., Molecular recognition mechanisms of thrombin (2005) J Thromb Haemost, 3, pp. 1861-1872
Fenton II, J.W., Thrombin specificity (1981) Ann N y Acad Sci, 370, pp. 468-495
Adams, T.E., Huntington, J.A., Thrombin-cofactor interactions: Structural insights into regulatory mechanisms (2006) Arterioscler Thromb Vasc Biol, 26, pp. 1738-1745
Welsby, I.J., Monroe, D.M., Lawson, J.H., Hoffmann, M., Recombinant activated factor VII and the anaesthetist (2005) Anaesthesia, 60, pp. 1203-1212
Bock, L.C., Griffin, L.C., Latham, J.A., Vermaas, E.H., Toole, J.J., Selection of single-stranded DNA molecules that bind and inhibit human thrombin (1992) Nature, 355, pp. 564-566
Russo Krauss, I., Pica, A., Merlino, A., Mazzarella, L., Sica, F., Duplex-quadruplex motifs in a peculiar structural organization cooperatively contribute to thrombin binding of a DNA aptamer (2013) Acta Crystallogr D, 69. , doi: 10.1107/S0907444913022269
Russo Krauss, I., Merlino, A., Randazzo, A., Novellino, E., Mazzarella, L., Sica, F., High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity (2012) Nucleic Acids Res, 40, pp. 8119-8128
MacAya, R.F., Schultze, P., Smith, F.W., Roe, J.A., Feigon, J., Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution (1993) Proc Natl Acad Sci USA, 90, pp. 3745-3749
Burge, S., Parkinson, G.N., Hazel, P., Todd, A.K., Neidle, S., Quadruplex DNA: Sequence, topology and structure (2006) Nucleic Acids Res, 34, pp. 5402-5415
Borbone, N., Bucci, M., Oliviero, G., Morelli, E., Amato, J., D'Atri, V., D'Errico, S., Piccialli, G., Investigating the role of T7 and T12 residues on the biological properties of thrombin-binding aptamer: Enhancement of anticoagulant activity by a single nucleobase modification (2012) J Med Chem, 55, pp. 10716-10728
Sagi, J., G-quadruplexes incorporating modified constituents: A review (2013) J Biomol Struct Dyn, , in press. doi: 10.1080/07391102.2013.775074
Bunka, D.H., Stockley, P.G., Aptamers come of age - At last (2006) Nat Rev Microbiol, 4, pp. 588-596
Nagatoishi, S., Sugimoto, N., Interaction of water with the G-quadruplex loop contributes to the binding energy of G-quadruplex to protein (2012) Mol BioSyst, 8, pp. 2766-2770
Figueiredo, A.C., Clement, C.C., Zakia, S., Gingold, J., Philipp, M., Pereira, P.J., Rational design and characterization of D-Phe-Pro-D-Arg-derived direct thrombin inhibitors (2012) PLoS ONE, 7, pp. e34354
Lima, L.M., Becker, C.F., Giesel, G.M., Marques, A.F., Cargnelutti, M.T., De Oliveira Neto, M., Monteiro, R.Q., Polikarpov, I., Structural and thermodynamic analysis of thrombin:suramin interaction in solution and crystal phases (2009) Biochim Biophys Acta, 1794, pp. 873-881
Davis, J.T., G-quartets 40 years later: From 5′-GMP to molecular biology and supramolecular chemistry (2004) Angew Chem Int Ed Engl, 43, pp. 668-698
Bonifacio, L., Church, F.C., Jarstfer, M.B., Effect of locked-nucleic acid on a biologically active G-quadruplex. A structure-activity relationship of the thrombin aptamer (2008) Int J Mol Sci, 9, pp. 422-433
Heckel, A., Mayer, G., Light regulation of aptamer activity: An anti-thrombin aptamer with caged thymidine nucleobases (2005) J Am Chem Soc, 127, pp. 822-823
Nagatoishi, S., Isono, N., Tsumoto, K., Sugimoto, N., Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding (2011) Biochimie, 93, pp. 1231-1238
Padmanabhan, K., Tulinsky, A., An ambiguous structure of a DNA 15-mer thrombin complex (1996) Acta Crystallogr D, 52, pp. 272-282
Otwinowski, Z., Minor, W., Processing of X-ray diffraction data collected in oscillation mode (1997) Methods in Enzymology, pp. 307-326. , In (Carter Jr.C.W., ed.), Academic Press, New York
McCoy, A.J., Grosse-Kunstleve, R.W., Adams, P.D., Winn, M.D., Storoni, L.C., Read, R.J., Phaser crystallographic software (2007) J Appl Crystallogr, 40, pp. 658-674
Brunger, A.T., Adams, P.D., Clore, G.M., Delano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Pannu, N.S., Crystallography and NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallogr D, 54, pp. 905-921
Vagin, A.A., Steiner, R.A., Lebedev, A.A., Potterton, L., McNicholas, S., Long, F., Murshudov, G.N., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr D, 60, pp. 2184-2195
Emsley, P., Lohkamp, B., Scott, W.G., Cowtan, K., Features and development of Coot (2010) Acta Crystallogr D, 66, pp. 486-501
Laskowski, R.A., Moss, D.S., Thornton, J.M., Main-chain bond lengths and bond angles in protein structures (1993) J Mol Biol, 231, pp. 1049-1067
Schrodinger, L., (2010) The PyMOL Molecular Graphics System, Version 1.3, , Schrödinger, LLC
Keefe, A. D., Pai, S., Ellington, A., Aptamers as therapeutics (2010) Nat Rev Drug Discov, 9, pp. 537-550
Dhar, S., Gu, F. X., Langer, R., Farokhzad, O. C., Lippard, S. J., Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt (IV) prodrug-PLGA-PEG nanoparticles (2008) Proc Natl Acad Sci USA, 105, pp. 17356-17361
Bunka, D. H., Platonova, O., Stockley, P. G., Development of aptamer therapeutics (2010) Curr Opin Pharmacol, 10, pp. 557-562
Bompiani, K. M., Woodruff, R. S., Becker, R. C., Nimjee, S. M., Sullenger, B. A., Antidote control of aptamer therapeutics: The road to a safer class of drug agents (2012) Curr Pharm Biotechnol, 13, pp. 1924-1934
Huntington, J. A., Molecular recognition mechanisms of thrombin (2005) J Thromb Haemost, 3, pp. 1861-1872
Fenton II, J. W., Thrombin specificity (1981) Ann N y Acad Sci, 370, pp. 468-495
Adams, T. E., Huntington, J. A., Thrombin-cofactor interactions: Structural insights into regulatory mechanisms (2006) Arterioscler Thromb Vasc Biol, 26, pp. 1738-1745
Welsby, I. J., Monroe, D. M., Lawson, J. H., Hoffmann, M., Recombinant activated factor VII and the anaesthetist (2005) Anaesthesia, 60, pp. 1203-1212
Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H., Toole, J. J., Selection of single-stranded DNA molecules that bind and inhibit human thrombin (1992) Nature, 355, pp. 564-566
MacAya, R. F., Schultze, P., Smith, F. W., Roe, J. A., Feigon, J., Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution (1993) Proc Natl Acad Sci USA, 90, pp. 3745-3749
Bunka, D. H., Stockley, P. G., Aptamers come of age - At last (2006) Nat Rev Microbiol, 4, pp. 588-596
Figueiredo, A. C., Clement, C. C., Zakia, S., Gingold, J., Philipp, M., Pereira, P. J., Rational design and characterization of D-Phe-Pro-D-Arg-derived direct thrombin inhibitors (2012) PLoS ONE, 7, pp. e34354
Lima, L. M., Becker, C. F., Giesel, G. M., Marques, A. F., Cargnelutti, M. T., De Oliveira Neto, M., Monteiro, R. Q., Polikarpov, I., Structural and thermodynamic analysis of thrombin: suramin interaction in solution and crystal phases (2009) Biochim Biophys Acta, 1794, pp. 873-881
Davis, J. T., G-quartets 40 years later: From 5 -GMP to molecular biology and supramolecular chemistry (2004) Angew Chem Int Ed Engl, 43, pp. 668-698
McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., Read, R. J., Phaser crystallographic software (2007) J Appl Crystallogr, 40, pp. 658-674
Brunger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Pannu, N. S., Crystallography and NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallogr D, 54, pp. 905-921
Vagin, A. A., Steiner, R. A., Lebedev, A. A., Potterton, L., McNicholas, S., Long, F., Murshudov, G. N., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr D, 60, pp. 2184-2195
Laskowski, R. A., Moss, D. S., Thornton, J. M., Main-chain bond lengths and bond angles in protein structures (1993) J Mol Biol, 231, pp. 1049-1067
Dissecting the contribution of thrombin exosite I in the recognition of thrombin binding aptamer
Thrombin plays a pivotal role in the coagulation cascade; therefore, it represents a primary target in the treatment of several blood diseases. The 15-mer DNA oligonucleotide 5-GGTTGGTGTGGTTGG-3, known as thrombin binding aptamer (TBA), is a highly potent inhibitor of the enzyme. TBA folds as an antiparallel chair-like G-quadruplex structure, with two G-tetrads surrounded by two TT loops on one side and a TGT loop on the opposite side. Previous crystallographic studies have shown that TBA binds thrombin exosite I by its TT loops, T3T4 and T12T13. In order to get a better understanding of the thrombin-TBA interaction, we have undertaken a crystallographic characterization of the complexes between thrombin and two TBA mutants, TBAT3 and TBAT12, which lack the nucleobase of T3 and T12, respectively. The structural details of the two complexes show that exosite I is actually split into two regions, which contribute differently to TBA recognition. These results provide the basis for a more rational design of new aptamers with improved therapeutic action.
Thrombin plays a pivotal role in the coagulation cascade; therefore, it represents a primary target in the treatment of several blood diseases. The 15-mer DNA oligonucleotide 5-GGTTGGTGTGGTTGG-3, known as thrombin binding aptamer (TBA), is a highly potent inhibitor of the enzyme. TBA folds as an antiparallel chair-like G-quadruplex structure, with two G-tetrads surrounded by two TT loops on one side and a TGT loop on the opposite side. Previous crystallographic studies have shown that TBA binds thrombin exosite I by its TT loops, T3T4 and T12T13. In order to get a better understanding of the thrombin-TBA interaction, we have undertaken a crystallographic characterization of the complexes between thrombin and two TBA mutants, TBAT3 and TBAT12, which lack the nucleobase of T3 and T12, respectively. The structural details of the two complexes show that exosite I is actually split into two regions, which contribute differently to TBA recognition. These results provide the basis for a more rational design of new aptamers with improved therapeutic action.
Dissecting the contribution of thrombin exosite I in the recognition of thrombin binding aptamer
Dissecting the contribution of thrombin exosite I in the recognition of thrombin binding aptamer
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Falanga AP, Terracciano M, Oliviero G, Roviello GN, Borbone N
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Greco F, Musumeci D, Borbone N, Falanga AP, D , #xerrico S, Terracciano M, Piccialli I, Roviello GN, Oliviero G
* Exploring the Parallel G-Quadruplex Nucleic Acid World: A Spectroscopic and Computational Investigation on the Binding of the c-myc Oncogene NHE III1 Region by the Phytochemical Polydatin (25 views)
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Impact Factor: 4.412
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Platella C, Capasso D, Riccardi C, Musumeci D, Dellagreca M, Montesarchio D
* Natural compounds from Juncus plants interacting with telomeric and oncogene G-quadruplex structures as potential anticancer agents (8 views)
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Impact Factor: 3.876
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Platella C, Gaglione R, Napolitano E, Arciello A, Pirota V, Doria F, Musumeci D, Montesarchio D
* DNA Binding Mode Analysis of a Core-Extended Naphthalene Diimide as a Conformation-Sensitive Fluorescent Probe of G-Quadruplex Structures (10 views)
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Balanikas E, Martinez-fernandez L, Improta R, Podbevšek P, Baldacchino G, Markovitsi D
* The Structural Duality of Nucleobases in Guanine Quadruplexes Controls Their Low-Energy Photoionization (12 views)
J Phys Chem Lett (ISSN: 1948-7185linking, 1948-7185electronic), 2021 Sep 2; 12(34): 8309-8313.
Impact Factor: 6.475
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Mulliri S, Laaksonen A, Spanu P, Farris R, Farci M, Mingoia F, Roviello GN, Mocci F
* Spectroscopic and In Silico Studies on the Interaction of Substituted Pyrazolo[1, 2-a]benzo[1, 2, 3, 4]tetrazine-3-one Derivatives with c-Myc G4-DNA (26 views)
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Impact Factor: 5.923
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Platella C, Napolitano E, Riccardi C, Musumeci D, Montesarchio D
* Disentangling the Structure-Activity Relationships of Naphthalene Diimides as Anticancer G-Quadruplex-Targeting Drugs (12 views)
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Impact Factor: 7.446
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Baroni S, Stefania R, Broche LM, Senn N, Lurie DJ, Ross PJ, Aime S, Geninatti Crich S
* A Novel Class of (1) H-MRI Contrast Agents Based on the Relaxation Enhancement Induced on Water Protons by (14) N-Containing Imidazole Moieties (74 views)
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Impact Factor: 12.959
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Green JA, Asha H, Santoro F, Improta R
* Excitonic Model for Strongly Coupled Multichromophoric Systems: The Electronic Circular Dichroism Spectra of Guanine Quadruplexes as Test Cases (82 views)
J Chem Theory Comput (ISSN: 1549-9618linking, 1549-9626electronic), 2021 Jan 12; 17(1): 405-415.
Impact Factor: 5.39
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Riccardi C, Napolitano E, Platella C, Musumeci D, Melone MAB, Montesarchio D
* Anti-VEGF DNA-based aptamers in cancer therapeutics and diagnostics (10 views)
Med Res Rev (ISSN: 0198-6325linking, 1098-1128electronic), 2021 Jan; 41(1): 464-506.
Impact Factor: 9.3
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Riccardi C, Napolitano E, Platella C, Musumeci D, Montesarchio D
* G-quadruplex-based aptamers targeting human thrombin: Discovery, chemical modifications and antithrombotic effects (12 views)
Pharmacol Ther (ISSN: 0163-7258linking), 2021 Jan; 217: 107649-107649.
Impact Factor: 11.127
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Ogloblina AM, Iaccarino N, Capasso D, Di Gaetano S, Garzarella EU, Dolinnaya NG, Yakubovskaya MG, Pagano B, Amato J, Randazzo A
* Toward G-Quadruplex-Based Anticancer Agents: Biophysical and Biological Studies of Novel AS1411 Derivatives (61 views)
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Impact Factor: 4.556
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Cusano AM, Aliberti A, Cusano A, Ruvo M
* Detection of small DNA fragments by biolayer interferometry (79 views)
Anal Biochem (ISSN: 0003-2697linking, 1096-0309electronic), 2020 Oct 15; 607: 113898-113898.
Impact Factor: 2.219
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Smaldone G, Ruggiero A, Balasco N, Vitagliano L
* Development of a Protein Scaffold for Arginine Sensing Generated through the Dissection of the Arginine-Binding Protein from Thermotoga maritima (72 views)
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Impact Factor: 4.556
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Ronda L, Tonelli A, Sogne E, Autiero I, Spyrakis F, Pellegrino S, Abbiati G, Maffioli E, Schulte C, Piano R, Cozzini P, Mozzarelli A, Bettati S, Clerici F, Milani P, Lenardi C, Tedeschi G, Gelmi ML
* Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus (85 views)
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Impact Factor: 3.275
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Platella C, Raucci U, Rega N, D’atri S, Levati L, Roviello GN, Fuggetta MP, Musumeci D, Montesarchio D
* Shedding light on the interaction of polydatin and resveratrol with G-quadruplex and duplex DNA: a biophysical, computational and biological approach (205 views)
Int J Biol Macromol (ISSN: 0141-8130linking, 1879-0003electronic), 2020 May 15; 151: 1163-1172.
Impact Factor: 5.162
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Martínez-fernández L, Esposito L, Improta R
* Studying the excited electronic states of guanine rich DNA quadruplexes by quantum mechanical methods: main achievements and perspectives (70 views)
Photochem Photobiol Sci (ISSN: 1474-905xlinking), 2020 Apr 15; 19(4): 436-444.
Impact Factor: 2.831
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Fik-jaskółka MA, Mkrtchyan AF, Saghyan AS, Palumbo R, Belter A, Hayriyan LA, Simonyan H, Roviello V, Roviello GN
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Impact Factor: 3.232
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Pelliccia S, Amato J, Capasso D, Di Gaetano S, Massarotti A, Piccolo M, Irace C, Tron GC, Pagano B, Randazzo A, Novellino E, Giustiniano M
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Impact Factor: 6.205
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Riccardi C, Capasso D, Rozza GM, Platella C, Montesarchio D, Di Gaetano S, Marzo T, Pratesi A, Messori L, Roviello GN, Musumeci D
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Impact Factor: 3.224
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Marzano M, Falanga AP, Marasco D, Borbone N, D , #errico S, Piccialli G, Roviello GN, Oliviero G
* Evaluation of an Analogue of the Marine ε-PLL Peptide as a Ligand of G-quadruplex DNA Structures (164 views) (PDF 61 views)
Mar Drugs (ISSN: 1660-3397linking), 2020 Jan 11; 18(1): 49-60.
Impact Factor: 4.073
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Martinez-fernandez L, Changenet P, Banyasz A, Gustavsson T, Markovitsi D, Improta R
* Comprehensive Study of Guanine Excited State Relaxation and Photoreactivity in G-quadruplexes (60 views)
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Impact Factor: 6.687
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Marzano M, Falanga AP, D'Errico S, Pinto B, Roviello GN, Piccialli G, Oliviero G, Borbone N
* New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes (279 views)
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Impact Factor: 3.06
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Martinez-fernandez L, Banyasz A, Markovitsi D, Improta R
* Topology Controls the Electronic Absorption and Delocalization of Electron Holes in Guanine Quadruplexes (393 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Oct 12; 24(57): 15185-15189.
Impact Factor: 5.476
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Smaldone G, Berisio R, Balasco N, D'Auria S, Vitagliano L, Ruggiero A
* Domain swapping dissection in Thermotoga maritima arginine binding protein: How structural flexibility may compensate destabilization (263 views)
Bba-Gen Subjects (ISSN: 1570-9639print, 1570-9639linking, 0005-2728), 2018 May 31; 1866(9): 952-962.
Impact Factor: 2.773
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Deiana M, Mettra B, Martinez-fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K
* Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe (415 views)
J Phys Chem Lett (ISSN: 1948-7185, 1948-7185electronic, 1948-7185linking), 2017 Dec 7; 8(23): 5915-5920.
Impact Factor: 8.709
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Banyasz A, Martinez-fernandez L, Balty C, Perron M, Douki T, Improta R, Markovitsi D
* Absorption of Low-Energy UV Radiation by Human Telomere G-Quadruplexes Generates Long-Lived Guanine Radical Cations (184 views)
J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 0002-7863print), 2017 Aug 2; 139(30): 10561-10568.
Impact Factor: 14.357
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Platella C, Guida S, Bonmassar L, Aquino A, Bonmassar E, Ravagnan G, Montesarchio D, Roviello GN, Musumeci D, Fuggetta MP
* Antitumour activity of resveratrol on human melanoma cells: a possible mechanism related to its interaction with malignant cell telomerase (290 views)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2017 Aug 02; 1861(11PtA): 2843-2851.
Impact Factor: 4.28
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D'Urso A, Randazzo R, Rizzo V, Gangemi CMA, Romanucci V, Zarrelli A, Tomaselli G, Milardi D, Borbone N, Purrello R, Piccialli G, Di Fabio G, Oliviero G
* Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms (284 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2017 Jul 14; 19(26): 17404-17410.
Impact Factor: 3.906
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Platella C, Riccardi C, Montesarchio D, Roviello GN, Musumeci D
* G-quadruplex-based aptamers against protein targets in therapy and diagnostics (307 views)
Bba-Gen Subjects (ISSN: 0304-4165, 0006-3002, 1872-8006electronic), 2017 May; 1861(5PtB): 1429-1447.
Impact Factor: 3.679
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Esposito V, Pirone L, Mayol L, Pedone E, Virgilio A, Galeone A
* Exploring the binding of d(GGGT)4 to the HIV-1 integrase: An approach to investigate G-quadruplex aptamer/target protein interactions (371 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016; 127: 19-22.
Impact Factor: 3.112
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Giaquinto M, Micco A, Aliberti A, Ricciardi A, Ruvo M, Cutolo A, Cusano A
* Microgel photonics and lab on fiber technology for advanced label-free fiber optic nanoprobes (211 views)
Proc Spie Int Soc Opt Eng (ISSN: 0277-786x9781510602199), 2016; 9916: N/D-N/D.
Impact Factor: 0.958
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Palladino P, Aura AM, Spoto G
* Surface plasmon resonance for the label-free detection of Alzheimer's β-amyloid peptide aggregation (528 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2016; 408(3): 849-854.
Impact Factor: 3.431
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Scognamiglio PL, Di Natale C, Leone M, Poletto M, Vitagliano L, Tell G, Marasco D
* G-quadruplex DNA recognition by nucleophosmin: new insights from protein dissection (570 views)
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Impact Factor: 5.353
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Romanucci V, Milardi D, Campagna T, Gaglione M, Messere A, D'Urso A, Crisafi E, La Rosa C, Zarrelli A, Balzarini J, Di Fabio G
* Synthesis, biophysical characterization and anti-HIV activity of d(TG(3)AG) Quadruplexes bearing hydrophobic tails at the 5 '-end (365 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 0968-0896linking), 2014 Feb 1; 22(3): 960-966.
Impact Factor: 2.793
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Russo Krauss I, Parkinson GN, Merlino A, Mattia CA, Randazzo A, Novellino E, Mazzarella L, Sica F
* A regular thymine tetrad and a peculiar supramolecular assembly in the first crystal structure of an all-LNA G-quadruplex (610 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2014 Feb; 70(2): 362-370.
Impact Factor: 7.232
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Giaquinto M, Micco A, Aliberti A, Ricciardi A, Ruvo M, Cutolo A, Cusano A
* Lab on fiber biosensors integrated with microgels (227 views)
Opt Infobase Conf Pap (ISSN: 9780-960038053), 2014; N/D: N/D-N/D.
Impact Factor: 0
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (471 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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D'Agata R, Spoto G
* Surface plasmon resonance imaging for nucleic acid detection (361 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 573-584.
Impact Factor: 3.578
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Russo Krauss I, Merlino A, Randazzo A, Novellino E, Mazzarella L, Sica F
* High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity (561 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962, 1362-4962electronic), 2012 Sep; 40(16): 8119-8128.
Impact Factor: 8.278
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D'Agata R, Spoto G
* Artificial DNA and surface plasmon resonance (536 views)
Artificial Dna Pna & Xna (ISSN: 1949-095x), 2012 Apr; 3(2): N/D-N/D.
Impact Factor: 0
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Musumeci D, Oliviero G, Roviello GN, Bucci EM, Piccialli G
* G-quadruplex-forming oligonucleotide conjugated to magnetic nanoparticles: Synthesis, characterization, and enzymatic stability assays (816 views)
Bioconjug Chem (ISSN: 1043-1802, 1520-4812, 1520-4812electronic), 2012 Mar 21; 23(3): 382-391.
Impact Factor: 4.58
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Magliulo M, Cella L, Pacelli R
* Novel technique radio frequency identification (RFID) based to manage patient flow in a radiotherapy department (309 views)
Proc Ieee Embs Int Conf Biomed Health Informatics (ISSN: 9781-4577, 9781-457721779), 2012; N/D: 972-975.
Impact Factor: 0.41
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Ruggiero A, Dattelbaum JD, Pennacchio A, Iozzino L, Staiano M, Luchansky MS, Der BS, Berisio R, D'Auria S, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein (506 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2011 Nov 1; 67(11): 1462-1465.
Impact Factor: 0.506
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA (434 views)
Lect Notes Electr Eng, 2010 May 15; 91LNEE: 485-488.
Impact Factor: 0
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging (425 views)
Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2010 May 15; 25(9): 2095-2100.
Impact Factor: 5.361
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D'Agata R, Corradini R, Grasso G, Marchelli R, Spoto G
* Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging (412 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2008 Sep 1; 9(13): 2067-2070.
Impact Factor: 3.322
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Staiano M, Saviano M, Herman P, Grycznyski Z, Fini C, Varriale A, Parracino A, Kold AB, Rossi M, D'Auria S
* Time-resolved fluorescence spectroscopy and molecular dynamics simulations point out the effects of pressure on the stability and dynamics of the porcine odorant-binding protein (653 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2008; 89(4): 284-291.
Impact Factor: 2.823
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* Exploring the relationship between G-quadruplex nucleic acids and plants: from plant G-quadruplex function to phytochemical G4 ligands with pharmaceutic potential (8 views)
Pharmaceutics, 2022 Nov 4; N/D: N/D-N/D.
Impact Factor: 6.525
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Greco F, Musumeci D, Borbone N, Falanga AP, D , #xerrico S, Terracciano M, Piccialli I, Roviello GN, Oliviero G
* Exploring the Parallel G-Quadruplex Nucleic Acid World: A Spectroscopic and Computational Investigation on the Binding of the c-myc Oncogene NHE III1 Region by the Phytochemical Polydatin (25 views)
Molecules (ISSN: 1420-3049linking), 2022 May 7; 27(9): 2997-3006.
Impact Factor: 4.412
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Platella C, Capasso D, Riccardi C, Musumeci D, Dellagreca M, Montesarchio D
* Natural compounds from Juncus plants interacting with telomeric and oncogene G-quadruplex structures as potential anticancer agents (8 views)
Org Biomol Chem (ISSN: 1477-0520linking), 2021 Nov 8; N/D: N/D-N/D.
Impact Factor: 3.876
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Platella C, Gaglione R, Napolitano E, Arciello A, Pirota V, Doria F, Musumeci D, Montesarchio D
* DNA Binding Mode Analysis of a Core-Extended Naphthalene Diimide as a Conformation-Sensitive Fluorescent Probe of G-Quadruplex Structures (10 views)
Int J Mol Sci (ISSN: 1422-0067linking), 2021 Sep 30; 22 (19 ): N/D-N/D.
Impact Factor:
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Balanikas E, Martinez-fernandez L, Improta R, Podbevšek P, Baldacchino G, Markovitsi D
* The Structural Duality of Nucleobases in Guanine Quadruplexes Controls Their Low-Energy Photoionization (12 views)
J Phys Chem Lett (ISSN: 1948-7185linking, 1948-7185electronic), 2021 Sep 2; 12(34): 8309-8313.
Impact Factor: 6.475
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Mulliri S, Laaksonen A, Spanu P, Farris R, Farci M, Mingoia F, Roviello GN, Mocci F
* Spectroscopic and In Silico Studies on the Interaction of Substituted Pyrazolo[1, 2-a]benzo[1, 2, 3, 4]tetrazine-3-one Derivatives with c-Myc G4-DNA (26 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic, 1661-6596), 2021 Jun 2; 22(11): 6028-6028.
Impact Factor: 5.923
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Platella C, Napolitano E, Riccardi C, Musumeci D, Montesarchio D
* Disentangling the Structure-Activity Relationships of Naphthalene Diimides as Anticancer G-Quadruplex-Targeting Drugs (12 views)
J Med Chem (ISSN: 0022-2623linking, 0022-2623print), 2021 Apr 8; 64(7): 3578-3603.
Impact Factor: 7.446
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Baroni S, Stefania R, Broche LM, Senn N, Lurie DJ, Ross PJ, Aime S, Geninatti Crich S
* A Novel Class of (1) H-MRI Contrast Agents Based on the Relaxation Enhancement Induced on Water Protons by (14) N-Containing Imidazole Moieties (74 views)
Angew Chem Int Ed (ISSN: 1433-7851linking, 1521-3773electronic), 2021 Feb 19; 60(8): 4208-4214.
Impact Factor: 12.959
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Green JA, Asha H, Santoro F, Improta R
* Excitonic Model for Strongly Coupled Multichromophoric Systems: The Electronic Circular Dichroism Spectra of Guanine Quadruplexes as Test Cases (82 views)
J Chem Theory Comput (ISSN: 1549-9618linking, 1549-9626electronic), 2021 Jan 12; 17(1): 405-415.
Impact Factor: 5.39
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Riccardi C, Napolitano E, Platella C, Musumeci D, Melone MAB, Montesarchio D
* Anti-VEGF DNA-based aptamers in cancer therapeutics and diagnostics (10 views)
Med Res Rev (ISSN: 0198-6325linking, 1098-1128electronic), 2021 Jan; 41(1): 464-506.
Impact Factor: 9.3
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Riccardi C, Napolitano E, Platella C, Musumeci D, Montesarchio D
* G-quadruplex-based aptamers targeting human thrombin: Discovery, chemical modifications and antithrombotic effects (12 views)
Pharmacol Ther (ISSN: 0163-7258linking), 2021 Jan; 217: 107649-107649.
Impact Factor: 11.127
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Ogloblina AM, Iaccarino N, Capasso D, Di Gaetano S, Garzarella EU, Dolinnaya NG, Yakubovskaya MG, Pagano B, Amato J, Randazzo A
* Toward G-Quadruplex-Based Anticancer Agents: Biophysical and Biological Studies of Novel AS1411 Derivatives (61 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic, 1661-6596), 2020 Oct 21; 21(20): N/D-N/D.
Impact Factor: 4.556
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Cusano AM, Aliberti A, Cusano A, Ruvo M
* Detection of small DNA fragments by biolayer interferometry (79 views)
Anal Biochem (ISSN: 0003-2697linking, 1096-0309electronic), 2020 Oct 15; 607: 113898-113898.
Impact Factor: 2.219
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Smaldone G, Ruggiero A, Balasco N, Vitagliano L
* Development of a Protein Scaffold for Arginine Sensing Generated through the Dissection of the Arginine-Binding Protein from Thermotoga maritima (72 views)
Int J Mol Sc (ISSN: 1422-0067linking, 1422-0067electronic, 1661-6596), 2020 Oct 12; 21(20): N/D-N/D.
Impact Factor: 4.556
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Ronda L, Tonelli A, Sogne E, Autiero I, Spyrakis F, Pellegrino S, Abbiati G, Maffioli E, Schulte C, Piano R, Cozzini P, Mozzarelli A, Bettati S, Clerici F, Milani P, Lenardi C, Tedeschi G, Gelmi ML
* Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus (85 views)
Sensors (ISSN: 1424-8220linking, 1424-3210), 2020 Sep 2; 20(17): N/D-N/D.
Impact Factor: 3.275
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Platella C, Raucci U, Rega N, D’atri S, Levati L, Roviello GN, Fuggetta MP, Musumeci D, Montesarchio D
* Shedding light on the interaction of polydatin and resveratrol with G-quadruplex and duplex DNA: a biophysical, computational and biological approach (205 views)
Int J Biol Macromol (ISSN: 0141-8130linking, 1879-0003electronic), 2020 May 15; 151: 1163-1172.
Impact Factor: 5.162
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Martínez-fernández L, Esposito L, Improta R
* Studying the excited electronic states of guanine rich DNA quadruplexes by quantum mechanical methods: main achievements and perspectives (70 views)
Photochem Photobiol Sci (ISSN: 1474-905xlinking), 2020 Apr 15; 19(4): 436-444.
Impact Factor: 2.831
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Fik-jaskółka MA, Mkrtchyan AF, Saghyan AS, Palumbo R, Belter A, Hayriyan LA, Simonyan H, Roviello V, Roviello GN
* Spectroscopic and SEM evidences for G4-DNA binding by a synthetic alkyne-containing amino acid with anticancer activity (210 views) (PDF 75 views)
Spectrochim Acta A (ISSN: 1386-1425linking), 2020 Mar 15; 229: 117884-117884.
Impact Factor: 3.232
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Pelliccia S, Amato J, Capasso D, Di Gaetano S, Massarotti A, Piccolo M, Irace C, Tron GC, Pagano B, Randazzo A, Novellino E, Giustiniano M
* Bio-Inspired Dual-Selective BCL-2/c-MYC G-Quadruplex Binders: Design, Synthesis, and Anticancer Activity of Drug-like Imidazo[2, 1-i]purine Derivatives (98 views)
J Med Chem (ISSN: 0022-2623linking, 0022-2623print), 2020 Mar 12; 63(5): 2035-2050.
Impact Factor: 6.205
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Riccardi C, Capasso D, Rozza GM, Platella C, Montesarchio D, Di Gaetano S, Marzo T, Pratesi A, Messori L, Roviello GN, Musumeci D
* Synthesis, DNA binding studies, and antiproliferative activity of novel Pt(II)- complexes with an L-alanyl-based ligand (261 views)
J Chem Res (ISSN: 0162-0134linking, 0162-0134print), 2020 Feb; 203: 110868-110868.
Impact Factor: 3.224
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Marzano M, Falanga AP, Marasco D, Borbone N, D , #errico S, Piccialli G, Roviello GN, Oliviero G
* Evaluation of an Analogue of the Marine ε-PLL Peptide as a Ligand of G-quadruplex DNA Structures (164 views) (PDF 61 views)
Mar Drugs (ISSN: 1660-3397linking), 2020 Jan 11; 18(1): 49-60.
Impact Factor: 4.073
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Martinez-fernandez L, Changenet P, Banyasz A, Gustavsson T, Markovitsi D, Improta R
* Comprehensive Study of Guanine Excited State Relaxation and Photoreactivity in G-quadruplexes (60 views)
J Phys Chem Lett (ISSN: 1948-7185linking, 1948-7185electronic), 2019 Nov 7; 10(21): 6873-6877.
Impact Factor: 6.687
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Marzano M, Falanga AP, D'Errico S, Pinto B, Roviello GN, Piccialli G, Oliviero G, Borbone N
* New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes (279 views)
Molecules (ISSN: 1420-3049linking, 1420-3049electronic), 2019 Feb 12; 24(3): 654-654.
Impact Factor: 3.06
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Martinez-fernandez L, Banyasz A, Markovitsi D, Improta R
* Topology Controls the Electronic Absorption and Delocalization of Electron Holes in Guanine Quadruplexes (393 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Oct 12; 24(57): 15185-15189.
Impact Factor: 5.476
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Smaldone G, Berisio R, Balasco N, D'Auria S, Vitagliano L, Ruggiero A
* Domain swapping dissection in Thermotoga maritima arginine binding protein: How structural flexibility may compensate destabilization (263 views)
Bba-Gen Subjects (ISSN: 1570-9639print, 1570-9639linking, 0005-2728), 2018 May 31; 1866(9): 952-962.
Impact Factor: 2.773
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Deiana M, Mettra B, Martinez-fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K
* Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe (415 views)
J Phys Chem Lett (ISSN: 1948-7185, 1948-7185electronic, 1948-7185linking), 2017 Dec 7; 8(23): 5915-5920.
Impact Factor: 8.709
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Banyasz A, Martinez-fernandez L, Balty C, Perron M, Douki T, Improta R, Markovitsi D
* Absorption of Low-Energy UV Radiation by Human Telomere G-Quadruplexes Generates Long-Lived Guanine Radical Cations (184 views)
J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 0002-7863print), 2017 Aug 2; 139(30): 10561-10568.
Impact Factor: 14.357
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Platella C, Guida S, Bonmassar L, Aquino A, Bonmassar E, Ravagnan G, Montesarchio D, Roviello GN, Musumeci D, Fuggetta MP
* Antitumour activity of resveratrol on human melanoma cells: a possible mechanism related to its interaction with malignant cell telomerase (290 views)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2017 Aug 02; 1861(11PtA): 2843-2851.
Impact Factor: 4.28
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D'Urso A, Randazzo R, Rizzo V, Gangemi CMA, Romanucci V, Zarrelli A, Tomaselli G, Milardi D, Borbone N, Purrello R, Piccialli G, Di Fabio G, Oliviero G
* Stabilization vs. destabilization of G-quadruplex superstructures: the role of the porphyrin derivative having spermine arms (284 views)
Phys Chem Chem Phys (ISSN: 1463-9076, 1463-3907, 1463-9084electronic), 2017 Jul 14; 19(26): 17404-17410.
Impact Factor: 3.906
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Platella C, Riccardi C, Montesarchio D, Roviello GN, Musumeci D
* G-quadruplex-based aptamers against protein targets in therapy and diagnostics (307 views)
Bba-Gen Subjects (ISSN: 0304-4165, 0006-3002, 1872-8006electronic), 2017 May; 1861(5PtB): 1429-1447.
Impact Factor: 3.679
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Esposito V, Pirone L, Mayol L, Pedone E, Virgilio A, Galeone A
* Exploring the binding of d(GGGT)4 to the HIV-1 integrase: An approach to investigate G-quadruplex aptamer/target protein interactions (371 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016; 127: 19-22.
Impact Factor: 3.112
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Giaquinto M, Micco A, Aliberti A, Ricciardi A, Ruvo M, Cutolo A, Cusano A
* Microgel photonics and lab on fiber technology for advanced label-free fiber optic nanoprobes (211 views)
Proc Spie Int Soc Opt Eng (ISSN: 0277-786x9781510602199), 2016; 9916: N/D-N/D.
Impact Factor: 0.958
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Palladino P, Aura AM, Spoto G
* Surface plasmon resonance for the label-free detection of Alzheimer's β-amyloid peptide aggregation (528 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2016; 408(3): 849-854.
Impact Factor: 3.431
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Scognamiglio PL, Di Natale C, Leone M, Poletto M, Vitagliano L, Tell G, Marasco D
* G-quadruplex DNA recognition by nucleophosmin: new insights from protein dissection (570 views)
Bba-Gen Subjects (ISSN: 0005-2728, 0006-3002print, 1570-9639print), 2014 Jun; 1840(6): 2050-2059.
Impact Factor: 5.353
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Romanucci V, Milardi D, Campagna T, Gaglione M, Messere A, D'Urso A, Crisafi E, La Rosa C, Zarrelli A, Balzarini J, Di Fabio G
* Synthesis, biophysical characterization and anti-HIV activity of d(TG(3)AG) Quadruplexes bearing hydrophobic tails at the 5 '-end (365 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 0968-0896linking), 2014 Feb 1; 22(3): 960-966.
Impact Factor: 2.793
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Russo Krauss I, Parkinson GN, Merlino A, Mattia CA, Randazzo A, Novellino E, Mazzarella L, Sica F
* A regular thymine tetrad and a peculiar supramolecular assembly in the first crystal structure of an all-LNA G-quadruplex (610 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2014 Feb; 70(2): 362-370.
Impact Factor: 7.232
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Giaquinto M, Micco A, Aliberti A, Ricciardi A, Ruvo M, Cutolo A, Cusano A
* Lab on fiber biosensors integrated with microgels (227 views)
Opt Infobase Conf Pap (ISSN: 9780-960038053), 2014; N/D: N/D-N/D.
Impact Factor: 0
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (471 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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D'Agata R, Spoto G
* Surface plasmon resonance imaging for nucleic acid detection (361 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 573-584.
Impact Factor: 3.578
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Russo Krauss I, Merlino A, Randazzo A, Novellino E, Mazzarella L, Sica F
* High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity (561 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962, 1362-4962electronic), 2012 Sep; 40(16): 8119-8128.
Impact Factor: 8.278
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D'Agata R, Spoto G
* Artificial DNA and surface plasmon resonance (536 views)
Artificial Dna Pna & Xna (ISSN: 1949-095x), 2012 Apr; 3(2): N/D-N/D.
Impact Factor: 0
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Musumeci D, Oliviero G, Roviello GN, Bucci EM, Piccialli G
* G-quadruplex-forming oligonucleotide conjugated to magnetic nanoparticles: Synthesis, characterization, and enzymatic stability assays (816 views)
Bioconjug Chem (ISSN: 1043-1802, 1520-4812, 1520-4812electronic), 2012 Mar 21; 23(3): 382-391.
Impact Factor: 4.58
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Magliulo M, Cella L, Pacelli R
* Novel technique radio frequency identification (RFID) based to manage patient flow in a radiotherapy department (309 views)
Proc Ieee Embs Int Conf Biomed Health Informatics (ISSN: 9781-4577, 9781-457721779), 2012; N/D: 972-975.
Impact Factor: 0.41
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Ruggiero A, Dattelbaum JD, Pennacchio A, Iozzino L, Staiano M, Luchansky MS, Der BS, Berisio R, D'Auria S, Vitagliano L
* Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein (506 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2011 Nov 1; 67(11): 1462-1465.
Impact Factor: 0.506
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA (434 views)
Lect Notes Electr Eng, 2010 May 15; 91LNEE: 485-488.
Impact Factor: 0
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D'Agata R, Corradini R, Ferretti C, Zanoli L, Gatti M, Marchelli R, Spoto G
* Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging (425 views)
Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2010 May 15; 25(9): 2095-2100.
Impact Factor: 5.361
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D'Agata R, Corradini R, Grasso G, Marchelli R, Spoto G
* Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging (412 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2008 Sep 1; 9(13): 2067-2070.
Impact Factor: 3.322
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Staiano M, Saviano M, Herman P, Grycznyski Z, Fini C, Varriale A, Parracino A, Kold AB, Rossi M, D'Auria S
* Time-resolved fluorescence spectroscopy and molecular dynamics simulations point out the effects of pressure on the stability and dynamics of the porcine odorant-binding protein (653 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2008; 89(4): 284-291.
Impact Factor: 2.823
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48 Records (43 excluding Abstracts).
Total impact factor: 221.008 (215.961 excluding Abstracts).
Total 5 year impact factor: 198.465 (192.343 excluding Abstracts).
Total impact factor: 221.008 (215.961 excluding Abstracts).
Total 5 year impact factor: 198.465 (192.343 excluding Abstracts).
465 views. Last view on Thursday 04 May 2023, 19:56:35
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