The multiple forms of bovine seminal ribonuclease: Structure and stability of a C-terminal swapped dimer (328 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2013 Nov 29; 587(23): 3755-3762.
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Paper type: Journal Article,
Impact factor: 3.341, 5-year impact factor: 3.47
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84887989317&partnerID=40&md5=69ab07525e4c7e1b7cd5d22f32504202
Keywords: Bovine Seminal Ribonuclease, C-Terminal 3d Domain Swapping, Cytotoxic Rnase, Hinge Peptide, Multiple Swapping, Dimer, Isoenzyme, Amino Acid Sequence, Article, Carboxy Terminal Sequence, Chemical Model, Controlled Study, Enzyme Stability, Enzyme Structure, Mutation, Nonhuman, Priority Journal, Protein Function, Protein Quaternary Structure, Seminal Plasma, X Ray, Artificial Dimer Of Bs-Rnase Obtained By Incubation In Acetic Acid Of Mbs And Lyophilisation, Artificial Dimer Of Staa-Bs-Rnase Obtained By Incubation In Acetic Acid Of Staa-Mbs And Lyophilisation, Bovine Pancreatic Ribonuclease (ec 3 1 2 27 5), Covalent Swapped Isomer Of Bs-Rnase, Covalent Unswapped Isomer Of Bs-Rnase, D(art)-Bs, D(art)-Staa-Bs, Dithiothreitol, G16s N17t P19a S20a Variant Of Bs-Rnase, G16s N17t P19a S20a Variant Of Mbs, Iodoacetamide, L28q Variant Of Mbs, L28q-Mbs, M×m, Monomeric Derivative Of Bs-Rnase With Cysteines 31 And 32 Either Linked To Glutathione Moieties Or Alkylated With Iodoacetamide, Ncd-Bs, Non-Covalent Swapped Dimer Of Bs-Rnase With Cysteines 31 And 32 Linked To Iodoacetamide, Obtained By Selective Reduction Of The Swapped Isoform And Iodoalkylation, P19a L28q Variant Of Bs-Rnase, P19a L28q Variant Of Mbs, Palq-Bs-Rnase, Palq-Mbs, R80s Variant Of Staa-Bs-Rnase, R80s Variant Of Staa-Mbs, R80s-Staa-Bs-Rnase, R80s-Staa-Mbs, Ribonuclease Inhibitor, Size-Exclusion Chromatography, Animals, Cattle, Crystallography, X-Ray, Endoribonucleases, Molecular Sequence Data, Protein Multimerization, Protein Structure, Tertiary, D (art)-Bs, D (art)-Staa-Bs,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples 'Federico II', via Cintia, 80126 Naples, Italy
Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, 80134 Naples, Italy
National Institute Biostructures and Biosystems, Inter-University Consortium, Viale Medaglie d'Oro 305, I-00136 Rome, Italy
References:
Liu, Y., Eisenberg, D., 3D domain swapping: As domains continue to swap (2002) Protein Sci., 11, pp. 1285-129
Vitagliano, L., Adinolfi, S., Sica, F., Merlino, A., Zagari, A., A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease (1999) J. Mol. Biol., 293, pp. 569-577
Youle R., .J., D'Alessio, G., (1997) Antitumor RNases In: Ribonucleases: Structures and Functions, pp. 491-514. , (G. D'Alessio and J.F. Riordan Eds.), Academic Press, New York
Sambashivan, S., Liu, Y., Sawaya, M.R., Gingery, M., Eisenberg, D., Amyloid-like fibrils of ribonuclease A with three-dimensional domain-swapped and native-like structure (2005) Nature, 437, pp. 266-269
Bennett, M.J., Sawaya, M.R., Eisenberg, D., Deposition diseases and 3D domain swapping (2006) Structure, 14, pp. 811-824
Koharudin, L.M., Liu, L., Gronenborn, A.M., Different 3D domain-swapped oligomeric cyanovirin-N structures suggest trapped folding intermediates (2013) Proc. Natl. Acad. Sci. USA, 110, pp. 7702-7707
Crestfield, A.M., Stein, W.H., Moore, S., On the preparation of bovine pancreatic ribonuclease A (1963) J. Biol. Chem., 238, pp. 618-621
Gotte, G., Mahmoud Helmy, A., Ercole, C., Spadaccini, R., Laurents, D.V., Double domain swapping in bovine seminal RNase: Formation of distinct N- and C-swapped tetramers and multimers with increasing biological activities (2012) PLoS One, 7, p. 46804
Piccoli, R., Tamburrini, M., Piccialli, G., Di Donato, A., Parente, A., The dual-mode quaternary structure of seminal RNase (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 1870-1874
Matousek, J., The effect of bovine seminal ribonuclease (AS RNase) on cells of Crocker tumour in mice (1973) Experientia, 29, pp. 858-859
Kim, J.S., Soucek, J., Matousek, J., Raines, R.T., Structural basis for the biological activities of bovine seminal ribonuclease (1995) J. Biol. Chem., 270, pp. 10525-10530
Merlino, A., Ercole, C., Picone, D., Pizzo, E., Mazzarella, L., The buried diversity of bovine seminal ribonuclease: Shape and cytotoxicity of the swapped non-covalent form of the enzyme (2008) J. Mol. Biol., 376, pp. 427-437
Giancola, C., Ercole, C., Fotticchia, I., Spadaccini, R., Pizzo, E., Structure-cytotoxicity relationships in bovine seminal ribonuclease: New insights from heat and chemical denaturation studies on variants (2011) FEBS J., 278, pp. 111-122
Sica, F., Di Fiore, A., Merlino, A., Mazzarella, L., Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease: An enzyme tailored to evade ribonuclease protein inhibitor (2004) J. Biol. Chem., 279, pp. 36753-36760
Kobe, B., Deisenhofer, J., Mechanism of ribonuclease inhibition by ribonuclease inhibitor protein based on the crystal structure of its complex with ribonuclease A (1996) J. Mol. Biol., 264, pp. 1028-1043
Merlino, A., Russo Krauss, I., Perillo, M., Mattia, C.A., Ercole, C., Toward an antitumor form of bovine pancreatic ribonuclease: The crystal structure of three noncovalent dimeric mutants (2009) Biopolymers, 91, pp. 1029-1037
Ercole, C., Colamarino, R.A., Pizzo, E., Fogolari, F., Spadaccini, R., Comparison of the structural and functional properties of RNase A and BS-RNase: A stepwise mutagenesis approach (2009) Biopolymers, 91, pp. 1009-1017
Spadaccini, R., Ercole, C., Gentile, M.A., Sanfelice, D., Boelens, R., NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: New insights for 3D domain swapping (2012) PLoS One, 7, p. 29076
Liu, Y., Hart, P.J., Schlunegger, M.P., Eisenberg, D., The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 3437-3442
Liu, Y., Gotte, G., Libonati, M., Eisenberg, D., A domain-swapped RNase A dimer with implications for amyloid formation (2001) Nat. Struct. Biol., 8, pp. 211-214
Cozza, G., Moro, S., Gotte, G., Elucidation of the ribonuclease A aggregation process mediated by 3D domain swapping: A computational approach reveals possible new multimeric structures (2008) Biopolymers, 89, pp. 26-39
Mazzarella, L., Vitagliano, L., Zagari, A., Swapping structural determinants of ribonucleases: An energetic analysis of the hinge peptide 16-22 (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 3799-3803
Di Donato, A., Cafaro, V., D'Alessio, G., Ribonuclease A can be transformed into a dimeric ribonuclease with antitumor activity (1994) J. Biol. Chem., 269, pp. 17394-17396
Picone, D., Di Fiore, A., Ercole, C., Franzese, M., Sica, F., The role of the hinge loop in domain swapping. The special case of bovine seminal ribonuclease (2005) J. Biol. Chem., 280, pp. 13771-13778
Ercole, C., Avitabile, F., Del Vecchio, P., Crescenzi, O., Tancredi, T., Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase (2003) Eur. J. Biochem., 270, pp. 4729-4735
Merlino, A., Picone, D., Ercole, C., Balsamo, A., Sica, F., Chain termini cross-talk in the swapping process of bovine pancreatic ribonuclease (2012) Biochimie, 94, pp. 1108-1118
Otwinowski, Z., Minor, W., Processing of X-ray diffraction data collected in oscillation mode (1997) Methods Enzymol., pp. 307-326. , Charles W. Carter Jr. Academic Press
Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., PHENIX: A comprehensive Python-based system for macromolecular structure solution (2010) Acta Crystallogr.
Vagin, A.A., Steiner, R.A., Lebedev, A.A., Potterton, L., McNicholas, S., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr.
Emsley, P., Lohkamp, B., Scott, W.G., Cowtan, K., Features and development of Coot (2010) Acta Crystallogr.
Laskowski, R.A., Macarthur, M.W., Moss, D.S., Thornton, J.M., PROCHECK: A program to check the stereochemical quality of protein structures (1993) J. Appl. Crystallogr., 26, pp. 283-291
(2010) The Pymol Molecular Graphics System, Version 1.3r1. Pymol, , http://wwwpymolorg, Schrodinger, LLC
Gotte, G., Bertoldi, M., Libonati, M., Structural versatility of bovine ribonuclease A. Distinct conformers of trimeric and tetrameric aggregates of the enzyme (1999) Eur. J. Biochem., 265, pp. 680-687
Merlino, A., Avella, G., Di Gaetano, S., Arciello, A., Piccoli, R., Structural features for the mechanism of antitumor action of a dimeric human pancreatic ribonuclease variant (2009) Protein Sci., 18, pp. 50-57
Pica, A., Merlino, A., Buell, A.K., Knowles, T.P.J., Pizzo, E., Three-dimensional domain swapping and supramolecular protein assembly: Insights from the X-ray structure of a dimeric swapped variant of human pancreatic RNase. Acta Crystallogr (2013) D, 69, pp. 2116-2123
Youle R., . J., D'Alessio, G., (1997) Antitumor RNases In: Ribonucleases: Structures and Functions, pp. 491-514. , (G. D'Alessio and J. F. Riordan Eds.), Academic Press, New York
Bennett, M. J., Sawaya, M. R., Eisenberg, D., Deposition diseases and 3D domain swapping (2006) Structure, 14, pp. 811-824
Koharudin, L. M., Liu, L., Gronenborn, A. M., Different 3D domain-swapped oligomeric cyanovirin-N structures suggest trapped folding intermediates (2013) Proc. Natl. Acad. Sci. USA, 110, pp. 7702-7707
Crestfield, A. M., Stein, W. H., Moore, S., On the preparation of bovine pancreatic ribonuclease A (1963) J. Biol. Chem., 238, pp. 618-621
Kim, J. S., Soucek, J., Matousek, J., Raines, R. T., Structural basis for the biological activities of bovine seminal ribonuclease (1995) J. Biol. Chem., 270, pp. 10525-10530
Liu, Y., Hart, P. J., Schlunegger, M. P., Eisenberg, D., The crystal structure of a 3D domain-swapped dimer of RNase A at a 2. 1-A resolution (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 3437-3442
Adams, P. D., Afonine, P. V., Bunkoczi, G., Chen, V. B., Davis, I. W., PHENIX: A comprehensive Python-based system for macromolecular structure solution (2010) Acta Crystallogr.
Vagin, A. A., Steiner, R. A., Lebedev, A. A., Potterton, L., McNicholas, S., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr.
Laskowski, R. A., Macarthur, M. W., Moss, D. S., Thornton, J. M., PROCHECK: A program to check the stereochemical quality of protein structures (1993) J. Appl. Crystallogr., 26, pp. 283-291
(2010) The Pymol Molecular Graphics System, Version 1. 3r1. Pymol, , http: //wwwpymolorg, Schrodinger, LLC
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2013 Nov 29; 587(23): 3755-3762.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.341, 5-year impact factor: 3.47
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84887989317&partnerID=40&md5=69ab07525e4c7e1b7cd5d22f32504202
Keywords: Bovine Seminal Ribonuclease, C-Terminal 3d Domain Swapping, Cytotoxic Rnase, Hinge Peptide, Multiple Swapping, Dimer, Isoenzyme, Amino Acid Sequence, Article, Carboxy Terminal Sequence, Chemical Model, Controlled Study, Enzyme Stability, Enzyme Structure, Mutation, Nonhuman, Priority Journal, Protein Function, Protein Quaternary Structure, Seminal Plasma, X Ray, Artificial Dimer Of Bs-Rnase Obtained By Incubation In Acetic Acid Of Mbs And Lyophilisation, Artificial Dimer Of Staa-Bs-Rnase Obtained By Incubation In Acetic Acid Of Staa-Mbs And Lyophilisation, Bovine Pancreatic Ribonuclease (ec 3 1 2 27 5), Covalent Swapped Isomer Of Bs-Rnase, Covalent Unswapped Isomer Of Bs-Rnase, D(art)-Bs, D(art)-Staa-Bs, Dithiothreitol, G16s N17t P19a S20a Variant Of Bs-Rnase, G16s N17t P19a S20a Variant Of Mbs, Iodoacetamide, L28q Variant Of Mbs, L28q-Mbs, M×m, Monomeric Derivative Of Bs-Rnase With Cysteines 31 And 32 Either Linked To Glutathione Moieties Or Alkylated With Iodoacetamide, Ncd-Bs, Non-Covalent Swapped Dimer Of Bs-Rnase With Cysteines 31 And 32 Linked To Iodoacetamide, Obtained By Selective Reduction Of The Swapped Isoform And Iodoalkylation, P19a L28q Variant Of Bs-Rnase, P19a L28q Variant Of Mbs, Palq-Bs-Rnase, Palq-Mbs, R80s Variant Of Staa-Bs-Rnase, R80s Variant Of Staa-Mbs, R80s-Staa-Bs-Rnase, R80s-Staa-Mbs, Ribonuclease Inhibitor, Size-Exclusion Chromatography, Animals, Cattle, Crystallography, X-Ray, Endoribonucleases, Molecular Sequence Data, Protein Multimerization, Protein Structure, Tertiary, D (art)-Bs, D (art)-Staa-Bs,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples 'Federico II', via Cintia, 80126 Naples, Italy
Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, 80134 Naples, Italy
National Institute Biostructures and Biosystems, Inter-University Consortium, Viale Medaglie d'Oro 305, I-00136 Rome, Italy
References:
Liu, Y., Eisenberg, D., 3D domain swapping: As domains continue to swap (2002) Protein Sci., 11, pp. 1285-129
Vitagliano, L., Adinolfi, S., Sica, F., Merlino, A., Zagari, A., A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease (1999) J. Mol. Biol., 293, pp. 569-577
Youle R., .J., D'Alessio, G., (1997) Antitumor RNases In: Ribonucleases: Structures and Functions, pp. 491-514. , (G. D'Alessio and J.F. Riordan Eds.), Academic Press, New York
Sambashivan, S., Liu, Y., Sawaya, M.R., Gingery, M., Eisenberg, D., Amyloid-like fibrils of ribonuclease A with three-dimensional domain-swapped and native-like structure (2005) Nature, 437, pp. 266-269
Bennett, M.J., Sawaya, M.R., Eisenberg, D., Deposition diseases and 3D domain swapping (2006) Structure, 14, pp. 811-824
Koharudin, L.M., Liu, L., Gronenborn, A.M., Different 3D domain-swapped oligomeric cyanovirin-N structures suggest trapped folding intermediates (2013) Proc. Natl. Acad. Sci. USA, 110, pp. 7702-7707
Crestfield, A.M., Stein, W.H., Moore, S., On the preparation of bovine pancreatic ribonuclease A (1963) J. Biol. Chem., 238, pp. 618-621
Gotte, G., Mahmoud Helmy, A., Ercole, C., Spadaccini, R., Laurents, D.V., Double domain swapping in bovine seminal RNase: Formation of distinct N- and C-swapped tetramers and multimers with increasing biological activities (2012) PLoS One, 7, p. 46804
Piccoli, R., Tamburrini, M., Piccialli, G., Di Donato, A., Parente, A., The dual-mode quaternary structure of seminal RNase (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 1870-1874
Matousek, J., The effect of bovine seminal ribonuclease (AS RNase) on cells of Crocker tumour in mice (1973) Experientia, 29, pp. 858-859
Kim, J.S., Soucek, J., Matousek, J., Raines, R.T., Structural basis for the biological activities of bovine seminal ribonuclease (1995) J. Biol. Chem., 270, pp. 10525-10530
Merlino, A., Ercole, C., Picone, D., Pizzo, E., Mazzarella, L., The buried diversity of bovine seminal ribonuclease: Shape and cytotoxicity of the swapped non-covalent form of the enzyme (2008) J. Mol. Biol., 376, pp. 427-437
Giancola, C., Ercole, C., Fotticchia, I., Spadaccini, R., Pizzo, E., Structure-cytotoxicity relationships in bovine seminal ribonuclease: New insights from heat and chemical denaturation studies on variants (2011) FEBS J., 278, pp. 111-122
Sica, F., Di Fiore, A., Merlino, A., Mazzarella, L., Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease: An enzyme tailored to evade ribonuclease protein inhibitor (2004) J. Biol. Chem., 279, pp. 36753-36760
Kobe, B., Deisenhofer, J., Mechanism of ribonuclease inhibition by ribonuclease inhibitor protein based on the crystal structure of its complex with ribonuclease A (1996) J. Mol. Biol., 264, pp. 1028-1043
Merlino, A., Russo Krauss, I., Perillo, M., Mattia, C.A., Ercole, C., Toward an antitumor form of bovine pancreatic ribonuclease: The crystal structure of three noncovalent dimeric mutants (2009) Biopolymers, 91, pp. 1029-1037
Ercole, C., Colamarino, R.A., Pizzo, E., Fogolari, F., Spadaccini, R., Comparison of the structural and functional properties of RNase A and BS-RNase: A stepwise mutagenesis approach (2009) Biopolymers, 91, pp. 1009-1017
Spadaccini, R., Ercole, C., Gentile, M.A., Sanfelice, D., Boelens, R., NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: New insights for 3D domain swapping (2012) PLoS One, 7, p. 29076
Liu, Y., Hart, P.J., Schlunegger, M.P., Eisenberg, D., The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 3437-3442
Liu, Y., Gotte, G., Libonati, M., Eisenberg, D., A domain-swapped RNase A dimer with implications for amyloid formation (2001) Nat. Struct. Biol., 8, pp. 211-214
Cozza, G., Moro, S., Gotte, G., Elucidation of the ribonuclease A aggregation process mediated by 3D domain swapping: A computational approach reveals possible new multimeric structures (2008) Biopolymers, 89, pp. 26-39
Mazzarella, L., Vitagliano, L., Zagari, A., Swapping structural determinants of ribonucleases: An energetic analysis of the hinge peptide 16-22 (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 3799-3803
Di Donato, A., Cafaro, V., D'Alessio, G., Ribonuclease A can be transformed into a dimeric ribonuclease with antitumor activity (1994) J. Biol. Chem., 269, pp. 17394-17396
Picone, D., Di Fiore, A., Ercole, C., Franzese, M., Sica, F., The role of the hinge loop in domain swapping. The special case of bovine seminal ribonuclease (2005) J. Biol. Chem., 280, pp. 13771-13778
Ercole, C., Avitabile, F., Del Vecchio, P., Crescenzi, O., Tancredi, T., Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase (2003) Eur. J. Biochem., 270, pp. 4729-4735
Merlino, A., Picone, D., Ercole, C., Balsamo, A., Sica, F., Chain termini cross-talk in the swapping process of bovine pancreatic ribonuclease (2012) Biochimie, 94, pp. 1108-1118
Otwinowski, Z., Minor, W., Processing of X-ray diffraction data collected in oscillation mode (1997) Methods Enzymol., pp. 307-326. , Charles W. Carter Jr. Academic Press
Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., PHENIX: A comprehensive Python-based system for macromolecular structure solution (2010) Acta Crystallogr.
Vagin, A.A., Steiner, R.A., Lebedev, A.A., Potterton, L., McNicholas, S., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr.
Emsley, P., Lohkamp, B., Scott, W.G., Cowtan, K., Features and development of Coot (2010) Acta Crystallogr.
Laskowski, R.A., Macarthur, M.W., Moss, D.S., Thornton, J.M., PROCHECK: A program to check the stereochemical quality of protein structures (1993) J. Appl. Crystallogr., 26, pp. 283-291
(2010) The Pymol Molecular Graphics System, Version 1.3r1. Pymol, , http://wwwpymolorg, Schrodinger, LLC
Gotte, G., Bertoldi, M., Libonati, M., Structural versatility of bovine ribonuclease A. Distinct conformers of trimeric and tetrameric aggregates of the enzyme (1999) Eur. J. Biochem., 265, pp. 680-687
Merlino, A., Avella, G., Di Gaetano, S., Arciello, A., Piccoli, R., Structural features for the mechanism of antitumor action of a dimeric human pancreatic ribonuclease variant (2009) Protein Sci., 18, pp. 50-57
Pica, A., Merlino, A., Buell, A.K., Knowles, T.P.J., Pizzo, E., Three-dimensional domain swapping and supramolecular protein assembly: Insights from the X-ray structure of a dimeric swapped variant of human pancreatic RNase. Acta Crystallogr (2013) D, 69, pp. 2116-2123
Youle R., . J., D'Alessio, G., (1997) Antitumor RNases In: Ribonucleases: Structures and Functions, pp. 491-514. , (G. D'Alessio and J. F. Riordan Eds.), Academic Press, New York
Bennett, M. J., Sawaya, M. R., Eisenberg, D., Deposition diseases and 3D domain swapping (2006) Structure, 14, pp. 811-824
Koharudin, L. M., Liu, L., Gronenborn, A. M., Different 3D domain-swapped oligomeric cyanovirin-N structures suggest trapped folding intermediates (2013) Proc. Natl. Acad. Sci. USA, 110, pp. 7702-7707
Crestfield, A. M., Stein, W. H., Moore, S., On the preparation of bovine pancreatic ribonuclease A (1963) J. Biol. Chem., 238, pp. 618-621
Kim, J. S., Soucek, J., Matousek, J., Raines, R. T., Structural basis for the biological activities of bovine seminal ribonuclease (1995) J. Biol. Chem., 270, pp. 10525-10530
Liu, Y., Hart, P. J., Schlunegger, M. P., Eisenberg, D., The crystal structure of a 3D domain-swapped dimer of RNase A at a 2. 1-A resolution (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 3437-3442
Adams, P. D., Afonine, P. V., Bunkoczi, G., Chen, V. B., Davis, I. W., PHENIX: A comprehensive Python-based system for macromolecular structure solution (2010) Acta Crystallogr.
Vagin, A. A., Steiner, R. A., Lebedev, A. A., Potterton, L., McNicholas, S., REFMAC5 dictionary: Organization of prior chemical knowledge and guidelines for its use (2004) Acta Crystallogr.
Laskowski, R. A., Macarthur, M. W., Moss, D. S., Thornton, J. M., PROCHECK: A program to check the stereochemical quality of protein structures (1993) J. Appl. Crystallogr., 26, pp. 283-291
(2010) The Pymol Molecular Graphics System, Version 1. 3r1. Pymol, , http: //wwwpymolorg, Schrodinger, LLC
The multiple forms of bovine seminal ribonuclease: Structure and stability of a C-terminal swapped dimer
Bovine seminal ribonuclease (BS-RNase) acquires an interesting anti-tumor activity associated with the swapping on the N-terminal. The first direct experimental evidence on the formation of a C-terminal swapped dimer (C-dimer) obtained from the monomeric derivative of BS-RNase, although under non-native conditions, is here reported. The X-ray model of this dimer reveals a quaternary structure different from that of the C-dimer of RNase A, due to the presence of three mutations in the hinge peptide 111-116. The mutations increase the hinge peptide flexibility and decrease the stability of the C-dimer against dissociation. The biological implications of the structural data are also discussed. Structure summary of protein interactions: BS-RNase and BS-RNase bind by x-ray crystallography (View interaction) BS-RNase and BS-RNase bind by molecular sieving (1, 2) BS-RNase and BS-RNase bind by blue native page (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Bovine seminal ribonuclease (BS-RNase) acquires an interesting anti-tumor activity associated with the swapping on the N-terminal. The first direct experimental evidence on the formation of a C-terminal swapped dimer (C-dimer) obtained from the monomeric derivative of BS-RNase, although under non-native conditions, is here reported. The X-ray model of this dimer reveals a quaternary structure different from that of the C-dimer of RNase A, due to the presence of three mutations in the hinge peptide 111-116. The mutations increase the hinge peptide flexibility and decrease the stability of the C-dimer against dissociation. The biological implications of the structural data are also discussed. Structure summary of protein interactions: BS-RNase and BS-RNase bind by x-ray crystallography (View interaction) BS-RNase and BS-RNase bind by molecular sieving (1, 2) BS-RNase and BS-RNase bind by blue native page (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
The multiple forms of bovine seminal ribonuclease: Structure and stability of a C-terminal swapped dimer
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The multiple forms of bovine seminal ribonuclease: Structure and stability of a C-terminal swapped dimer
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Impact Factor: 3.415
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Picone D, Di Fiore A, Ercole C, Franzese M, Sica F, Tomaselli S, Mazzarella L
* The role of the hinge loop in domain swapping - The special case of bovine seminal ribonuclease (367 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Apr 8; 280(14): 13771-13778.
Impact Factor: 5.854
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Sica F, Di Fiore A, Merlino A, Mazzarella L
* Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease - An enzyme tailored to evade ribonuclease protein inhibitor (443 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2004 Sep 27; 279(35): 36753-36760.
Impact Factor: 6.355
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Merlino A, Vitagliano L, Sica F, Zagari A, Mazzarella L
* Population shift vs induced fit: The case of bovine seminal ribonuclease swapping dimer (384 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2004 Apr 15; 73(6): 689-695.
Impact Factor: 2.863
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Berisio R, Sica F, De Lorenzo C, Di Fiore A, Piccoli R, Zagari A, Mazzarella L
* Crystal Structure Of The Dimeric Un-Swapped Form Of Bovine Seminal Ribonuclease (383 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2003 Nov 6; 554(1-2): 105-110.
Impact Factor: 3.609
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Sica F, Di Fiore A, Zagari A, Mazzarella L
* The unswapped chain of bovine seminal ribonuclease: Crystal structure of the free and liganded monomeric derivative (355 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2003 Sep 1; 52(2): 263-271.
Impact Factor: 4.313
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Spalletti-cernia D, Sorrentino R, Di Gaetano S, Arciello A, Garbi C, Piccoli R, D'Alessio G, Vecchio G, Laccetti P, Santoro M
* Antineoplastic ribonucleases selectively kill thyroid carcinoma cells via caspase-mediated induction of apoptosis (384 views)
Journal Of Clinical Endocrinology & Metabolism (ISSN: 0021-972x), 2003 Jun; 88(6): 2900-2907.
Impact Factor: 5.873
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Vitagliano L, Adinolfi S, Sica F, Merlino A, Zagari A, Mazzarella L
A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease (381 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 1999 Oct 29; 293(3): 569-577.
Impact Factor: 5.501
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Sica F, Adinolfi S, Berisio R, De Lorenzo C, Mazzarella L, Piccoli R, Vitagliano L, Zagari A
Crystallization of multiple forms of bovine seminal ribonuclease in the liganded and unliganded state (282 views)
J Cryst Growth (ISSN: 0022-0248), 1999; 196(2-4): 305-312.
Impact Factor: 1.492
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Vitagliano L, Adinolfi S, Riccio A, Sica F, Zagari A, Mazzarella L
Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2 ', 5 ')adenosine (425 views)
Protein Sci (ISSN: 0961-8368, 1469-896xelectronic), 1998 Sep; 7(8): 1691-1699.
Impact Factor: 4.44
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Sica F, Adinolfi S, Vitagliano L, Zagari A, Capasso S, Mazzarella L
Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions (293 views)
J Cryst Growth (ISSN: 0022-0248), 1996; 168(1-4): 192-197.
Impact Factor: 1.259
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* Enforcing the positive charge of N-termini enhances membrane interaction and antitumor activity of bovine seminal ribonuclease (478 views)
Bba-Gen Subjects (ISSN: 0005-2736, 0006-3002, 0925-4439), 2011 Dec; 1808(12): 3007-3015.
Impact Factor: 3.99
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Merlino A, Russo Krauss I, Perillo M, Mattia CA, Ercole C, Picone D, Vergara A, Sica F
* Toward an Antitumor Form of Bovine Pancreatic Ribonuclease: The Crystal Structure of Three Noncovalent Dimeric Mutants (399 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1029-1037.
Impact Factor: 2.605
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Merlino A, Ercole C, Picone D, Pizzo E, Mazzarella L, Sica F
* The buried diversity of bovine seminal ribonuclease: Shape and cytotoxicity of the swapped non-covalent form of the enzyme (451 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2008 Feb 15; 376(2): 427-437.
Impact Factor: 4.146
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Naddeo M, Vitagliano L, Russo A, Gotte G, D'Alessio G, Sorrentino S
* Interactions of the cytotoxic RNase A dimers with the cytosolic ribonuclease inhibitor (362 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2005 May 9; 579(12): 2663-2668.
Impact Factor: 3.415
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Picone D, Di Fiore A, Ercole C, Franzese M, Sica F, Tomaselli S, Mazzarella L
* The role of the hinge loop in domain swapping - The special case of bovine seminal ribonuclease (367 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2005 Apr 8; 280(14): 13771-13778.
Impact Factor: 5.854
View Export to BibTeX Export to EndNote
Sica F, Di Fiore A, Merlino A, Mazzarella L
* Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease - An enzyme tailored to evade ribonuclease protein inhibitor (443 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2004 Sep 27; 279(35): 36753-36760.
Impact Factor: 6.355
View Export to BibTeX Export to EndNote
Merlino A, Vitagliano L, Sica F, Zagari A, Mazzarella L
* Population shift vs induced fit: The case of bovine seminal ribonuclease swapping dimer (384 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2004 Apr 15; 73(6): 689-695.
Impact Factor: 2.863
View Export to BibTeX Export to EndNote
Berisio R, Sica F, De Lorenzo C, Di Fiore A, Piccoli R, Zagari A, Mazzarella L
* Crystal Structure Of The Dimeric Un-Swapped Form Of Bovine Seminal Ribonuclease (383 views)
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2003 Nov 6; 554(1-2): 105-110.
Impact Factor: 3.609
View Export to BibTeX Export to EndNote
Sica F, Di Fiore A, Zagari A, Mazzarella L
* The unswapped chain of bovine seminal ribonuclease: Crystal structure of the free and liganded monomeric derivative (355 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2003 Sep 1; 52(2): 263-271.
Impact Factor: 4.313
View Export to BibTeX Export to EndNote
Spalletti-cernia D, Sorrentino R, Di Gaetano S, Arciello A, Garbi C, Piccoli R, D'Alessio G, Vecchio G, Laccetti P, Santoro M
* Antineoplastic ribonucleases selectively kill thyroid carcinoma cells via caspase-mediated induction of apoptosis (384 views)
Journal Of Clinical Endocrinology & Metabolism (ISSN: 0021-972x), 2003 Jun; 88(6): 2900-2907.
Impact Factor: 5.873
View Export to BibTeX Export to EndNote
Vitagliano L, Adinolfi S, Sica F, Merlino A, Zagari A, Mazzarella L
A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease (381 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 1999 Oct 29; 293(3): 569-577.
Impact Factor: 5.501
View Export to BibTeX Export to EndNote
Sica F, Adinolfi S, Berisio R, De Lorenzo C, Mazzarella L, Piccoli R, Vitagliano L, Zagari A
Crystallization of multiple forms of bovine seminal ribonuclease in the liganded and unliganded state (282 views)
J Cryst Growth (ISSN: 0022-0248), 1999; 196(2-4): 305-312.
Impact Factor: 1.492
View Export to BibTeX Export to EndNote
Vitagliano L, Adinolfi S, Riccio A, Sica F, Zagari A, Mazzarella L
Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2 ', 5 ')adenosine (425 views)
Protein Sci (ISSN: 0961-8368, 1469-896xelectronic), 1998 Sep; 7(8): 1691-1699.
Impact Factor: 4.44
View Export to BibTeX Export to EndNote
Sica F, Adinolfi S, Vitagliano L, Zagari A, Capasso S, Mazzarella L
Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions (293 views)
J Cryst Growth (ISSN: 0022-0248), 1996; 168(1-4): 192-197.
Impact Factor: 1.259
View Export to BibTeX Export to EndNote
14 Records (14 excluding Abstracts).
Total impact factor: 55.715 (55.715 excluding Abstracts).
Total 5 year impact factor: 46.597 (46.597 excluding Abstracts).
Total impact factor: 55.715 (55.715 excluding Abstracts).
Total 5 year impact factor: 46.597 (46.597 excluding Abstracts).
328 views. Last view on Saturday 14 January 2023, 17:52:38
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