Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy (467 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.191, 5-year impact factor: 3.282
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84879048177&partnerID=40&md5=512e21ff48bb8d4814830568aa75948a
Keywords: Chemical Shift Perturbation, Death Domain, Ped Pea15 Protein, Protein-Protein Interaction, D4 Alpha Protein, Phospholipase D1, Phosphoprotein, Phosphoprotein Enriched In Astrocyte 15, Phosphoprotein Enriched In Diabetes, Protein Kinase C Alpha, Synthetic Peptide, Unclassified Drug, Human, Peptide Fragment, Signal Peptide, Allosterism, Article, Binding Site, Controlled Study, Enzyme Activity, Enzyme Linked Immunosorbent Assay, Nuclear Magnetic Resonance Spectroscopy, Priority Journal, Protein Analysis, Protein Binding, Protein Expression, Protein Protein Interaction, Chemistry, Metabolism, Protein Conformation, Enzyme-Linked Immunosorbent Assay, Intracellular Signaling Peptides And Proteins, Intracellular Signaling Peptides And Proteins Chemistry Metabolism, Peptide Fragments Chemistry Metabolism, Phospholipase D Chemistry Metabolism, Phosphoproteins Chemistry Metabolism,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Napoli, Italy
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43, 81100
References:
Araujo, H., Danziger, N., Cordier, J., Glowinski, J., Chneiweiss, H., Characterization of PEA-15, a major substrate for protein kinase C in astrocytes (1993) Journal of Biological Chemistry, 268 (8), pp. 5911-592
Estelles, A., Yokoyama, M., Nothias, F., Vincent, J.-D., Glowinski, J., Vernier, P., Chneiweiss, H., The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human (1996) Journal of Biological Chemistry, 271 (25), pp. 14800-14806. , DOI 10.1074/jbc.271.25.14800
Hill, J.M., Vaidyanathan, H., Ramos, J.W., Ginsberg, M.H., Werner, M.H., Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain (2002) EMBO Journal, 21 (23), pp. 6494-6504. , DOI 10.1093/emboj/cdf641
Twomey, E.C., Wei, Y., High-definition NMR structure of PED/PEA-15 death effector domain reveals details of key polar side chain interactions (2012) Biochem. Biophys. Res. Commun., 424, pp. 141-146
Fiory, F., Formisano, P., Perruolo, G., Beguinot, F., Frontiers: PED/PEA-15, a multifunctional protein controlling cell survival and glucose metabolism (2009) Am. J. Physiol. Endocrinol. Metab., 297, pp. 592-601
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO Journal, 17 (14), pp. 3858-3866. , DOI 10.1093/emboj/17.14.3858
Valentino, R., Lupoli, G.A., Raciti, G.A., Oriente, F., Farinaro, E., Della Valle, E., Salomone, M., Beguinot, F., The PEA15 gene is overexpressed and related to insulin resistance in healthy first-degree relatives of patients with type 2 diabetes (2006) Diabetologia, 49 (12), pp. 3058-3066. , DOI 10.1007/s00125-006-0455-5
Ungaro, P., Teperino, R., Mirra, P., Cassese, A., Fiory, F., Perruolo, G., Miele, C., Beguinot, F., Molecular cloning and characterization of the human PED/PEA-15 gene promoter reveals antagonistic regulation by HNF-4alpha and COUP-TFII (2008) J. Biol. Chem., 283, pp. 30970-30979
Vigliotta, G., Miele, C., Santopietro, S., Portella, G., Perfetti, A., Maitan, M.A., Cassese, A., Beguinot, F., Overexpression of the ped/pea-15 gene causes diabetes by impairing glucose-stimulated insulin secretion in addition to insulin action (2004) Molecular and Cellular Biology, 24 (11), pp. 5005-5015. , DOI 10.1128/MCB.24.11.5005-5015.2004
Miele, C., Raciti, G.A., Cassese, A., Romano, C., Giacco, F., Oriente, F., Paturzo, F., Beguinot, F., PED/PEA-15 regulates glucose-induced insulin secretion by restraining potassium channel expression in pancreatic beta-cells (2007) Diabetes, 56 (3), pp. 622-633. , http://diabetes.diabetesjournals.org/cgi/reprint/56/3/622.pdf, DOI 10.2337/db06-1260
Zhang, Y., Redina, O., Altshuller, Y.M., Yamazaki, M., Ramos, J., Chneiweiss, H., Kanaho, Y., Frohman, M.A., Regulation of expression of phospholipase D1 and D2 by PEA-15, a novel protein that interacts with them (2000) J. Biol. Chem., 275, pp. 35224-35232
Viparelli, F., Cassese, A., Doti, N., Paturzo, F., Marasco, D., Dathan, N.A., Monti, S.M., Ruvo, M., Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (2008) J. Biol. Chem., 283, pp. 21769-21778
Doti, N., Cassese, A., Marasco, D., Paturzo, F., Sabatella, M., Viparelli, F., Dathan, N., Ruvo, M., Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (2010) Mol. Biosyst., 6, pp. 2039-2048
Mayer, M., Meyer, B., Group epitope mapping by saturation transfer difference NMR to identify segments of a ligand in direct contact with a protein receptor (2001) Journal of the American Chemical Society, 123 (25), pp. 6108-6117. , DOI 10.1021/ja0100120
Farina, B., Pirone, L., Russo, L., Viparelli, F., Doti, N., Pedone, C., Pedone, E.M., Fattorusso, R., NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (2010) FEBS J., 277, pp. 4229-4240
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214
Pace, C.N., Vajdos, F., Fee, L., Grimsley, G., Gray, T., How to measure and predict the molar absorption coefficient of a protein (1995) Protein Sci., 4, pp. 2411-2423
Stan Tsai, C., (2007) Biomacromolecules: Introduction to Structure, Function and Informatics, pp. 183-209. , J. Wiley and sons, Hoboken, NJ
Marion, D., Ikura, M., Tschudin, R., Bax, A., Rapid recording of 2D NMR spectra without phase-cycling. Application to the study of hydrogen exchange in proteins (1989) J. Magn. Reson., 85, pp. 393-399
Piotto, M., Saudek, V., Sklenar, V., Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions (1992) J. Biomol. NMR, 2, pp. 661-665
Sklenar, V., Piotto, M., Leppik, R., Saudek, V., Gradient-tailored water suppression for 1H-15N HSQC experiments optimized to retain full sensitivity (1993) J. Magn. Reson. A, 102, pp. 241-245
Braunschweiler, L., Ernst, R.R., Coherence transfer by isotropic mixing: Application to proton correlation spectroscopy (1983) J. Magn. Reson., 53, pp. 521-528
Anil-Kumar, R.R., Wüthrich Ernst, K., A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules (1980) Biochem. Biophys. Res. Commun., 95, pp. 1-6
Hwang, T.L., Shaka, A.J., Water suppression that works: Excitation sculpting using arbitrary wave-forms and pulsed-field gradients (1995) J. Magn. Reson. A, 112, pp. 275-279
Dalvit, C., Efficient multiple-solvent suppression for the study of the interactions of organic solvents with biomolecules (1998) Journal of Biomolecular NMR, 11 (4), pp. 437-444
(2012) ACD/NMR Processor Freeware, , www.acdlabs.com, Version 12.01, Advanced Chemistry Development, Inc., Toronto, ON, Canada
Keller, R.L.J., (2004) The Computer Aided Resonance Assignment Tutorial, , CANTINA Verlag
Gunasekaran, K., Ma, B., Nussinov, R., Is allostery an intrinsic property of all dynamic proteins? (2004) Proteins: Structure, Function and Genetics, 57 (3), pp. 433-443. , DOI 10.1002/prot.20232
Niu, X., Chen, Q., Zhang, J., Shen, W., Shi, Y., Wu, J., Interesting structural and dynamical behaviors exhibited by the AF-6 PDZ domain upon Bcr peptide binding (2007) Biochemistry, 46 (51), pp. 15042-15053. , DOI 10.1021/bi701303p
Twomey, E.C., Cordasco, D.F., Wei, Y., Profound conformational changes of PED/PEA-15 in ERK2 complex revealed by NMR backbone dynamics (2012) BBA Prot. and Proteomics, 1824, pp. 1382-1393
Kersse, K., Verspurten, J., VandenBerghe, T., Vandenabeele, P., The death-fold superfamily of homotypic interaction motifs (2011) Trends Biochem. Sci., 36, pp. 541-552
Li, F.Y., Jeffrey, P.D., Yu, J.W., Shi, Y., Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition (2006) J. Biol. Chem., 281, pp. 2960-2968
Yang, J.K., Wang, L., Zheng, L., Wan, F., Ahmed, M., Lenardo, M.J., Wu, H., Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition (2005) Molecular Cell, 20 (6), pp. 939-949. , DOI 10.1016/j.molcel.2005.10.023, PII S1097276505017181
Bagneris, C., Ageichik, A.V., Cronin, N., Wallace, B., Collins, M., Boshoff, C., Waksman, G., Barrett, T., Crystal Structure of a vFlip-IKKgamma Complex: Insights into Viral Activation of the IKK Signalosome (2008) Molecular Cell, 30 (5), pp. 620-631. , DOI 10.1016/j.molcel.2008.04.029, PII S1097276508003602
Qin, H., Srinivasula, S.M., Wu, G., Fernandes-Alnemri, T., Alnemri, E.S., Shi, Y., Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1 (1999) Nature, 399, pp. 547-555
Srimathi, T., Robbins, S.L., Dubas, R.L., Chang, H., Cheng, H., Roder, H., Park, Y.C., Mapping of POP1-binding site on pyrin domain of ASC (2008) J. Biol. Chem., 283, pp. 15390-15398
Kam, Y., Exton, J.H., Dimerization of phospholipase D isozymes (2002) Biochemical and Biophysical Research Communications, 290 (1), pp. 375-380. , DOI 10.1006/bbrc.2001.6146
Koradi, R., Billeter, M., Wuthrich, K., MOLMOL: A program for display and analysis of macromolecular structures (1996) Journal of Molecular Graphics, 14 (1), pp. 51-55. , DOI 10.1016/0263-7855(96)00009-4
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.191, 5-year impact factor: 3.282
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84879048177&partnerID=40&md5=512e21ff48bb8d4814830568aa75948a
Keywords: Chemical Shift Perturbation, Death Domain, Ped Pea15 Protein, Protein-Protein Interaction, D4 Alpha Protein, Phospholipase D1, Phosphoprotein, Phosphoprotein Enriched In Astrocyte 15, Phosphoprotein Enriched In Diabetes, Protein Kinase C Alpha, Synthetic Peptide, Unclassified Drug, Human, Peptide Fragment, Signal Peptide, Allosterism, Article, Binding Site, Controlled Study, Enzyme Activity, Enzyme Linked Immunosorbent Assay, Nuclear Magnetic Resonance Spectroscopy, Priority Journal, Protein Analysis, Protein Binding, Protein Expression, Protein Protein Interaction, Chemistry, Metabolism, Protein Conformation, Enzyme-Linked Immunosorbent Assay, Intracellular Signaling Peptides And Proteins, Intracellular Signaling Peptides And Proteins Chemistry Metabolism, Peptide Fragments Chemistry Metabolism, Phospholipase D Chemistry Metabolism, Phosphoproteins Chemistry Metabolism,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Napoli, Italy
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43, 81100
References:
Araujo, H., Danziger, N., Cordier, J., Glowinski, J., Chneiweiss, H., Characterization of PEA-15, a major substrate for protein kinase C in astrocytes (1993) Journal of Biological Chemistry, 268 (8), pp. 5911-592
Estelles, A., Yokoyama, M., Nothias, F., Vincent, J.-D., Glowinski, J., Vernier, P., Chneiweiss, H., The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human (1996) Journal of Biological Chemistry, 271 (25), pp. 14800-14806. , DOI 10.1074/jbc.271.25.14800
Hill, J.M., Vaidyanathan, H., Ramos, J.W., Ginsberg, M.H., Werner, M.H., Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain (2002) EMBO Journal, 21 (23), pp. 6494-6504. , DOI 10.1093/emboj/cdf641
Twomey, E.C., Wei, Y., High-definition NMR structure of PED/PEA-15 death effector domain reveals details of key polar side chain interactions (2012) Biochem. Biophys. Res. Commun., 424, pp. 141-146
Fiory, F., Formisano, P., Perruolo, G., Beguinot, F., Frontiers: PED/PEA-15, a multifunctional protein controlling cell survival and glucose metabolism (2009) Am. J. Physiol. Endocrinol. Metab., 297, pp. 592-601
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO Journal, 17 (14), pp. 3858-3866. , DOI 10.1093/emboj/17.14.3858
Valentino, R., Lupoli, G.A., Raciti, G.A., Oriente, F., Farinaro, E., Della Valle, E., Salomone, M., Beguinot, F., The PEA15 gene is overexpressed and related to insulin resistance in healthy first-degree relatives of patients with type 2 diabetes (2006) Diabetologia, 49 (12), pp. 3058-3066. , DOI 10.1007/s00125-006-0455-5
Ungaro, P., Teperino, R., Mirra, P., Cassese, A., Fiory, F., Perruolo, G., Miele, C., Beguinot, F., Molecular cloning and characterization of the human PED/PEA-15 gene promoter reveals antagonistic regulation by HNF-4alpha and COUP-TFII (2008) J. Biol. Chem., 283, pp. 30970-30979
Vigliotta, G., Miele, C., Santopietro, S., Portella, G., Perfetti, A., Maitan, M.A., Cassese, A., Beguinot, F., Overexpression of the ped/pea-15 gene causes diabetes by impairing glucose-stimulated insulin secretion in addition to insulin action (2004) Molecular and Cellular Biology, 24 (11), pp. 5005-5015. , DOI 10.1128/MCB.24.11.5005-5015.2004
Miele, C., Raciti, G.A., Cassese, A., Romano, C., Giacco, F., Oriente, F., Paturzo, F., Beguinot, F., PED/PEA-15 regulates glucose-induced insulin secretion by restraining potassium channel expression in pancreatic beta-cells (2007) Diabetes, 56 (3), pp. 622-633. , http://diabetes.diabetesjournals.org/cgi/reprint/56/3/622.pdf, DOI 10.2337/db06-1260
Zhang, Y., Redina, O., Altshuller, Y.M., Yamazaki, M., Ramos, J., Chneiweiss, H., Kanaho, Y., Frohman, M.A., Regulation of expression of phospholipase D1 and D2 by PEA-15, a novel protein that interacts with them (2000) J. Biol. Chem., 275, pp. 35224-35232
Viparelli, F., Cassese, A., Doti, N., Paturzo, F., Marasco, D., Dathan, N.A., Monti, S.M., Ruvo, M., Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (2008) J. Biol. Chem., 283, pp. 21769-21778
Doti, N., Cassese, A., Marasco, D., Paturzo, F., Sabatella, M., Viparelli, F., Dathan, N., Ruvo, M., Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (2010) Mol. Biosyst., 6, pp. 2039-2048
Mayer, M., Meyer, B., Group epitope mapping by saturation transfer difference NMR to identify segments of a ligand in direct contact with a protein receptor (2001) Journal of the American Chemical Society, 123 (25), pp. 6108-6117. , DOI 10.1021/ja0100120
Farina, B., Pirone, L., Russo, L., Viparelli, F., Doti, N., Pedone, C., Pedone, E.M., Fattorusso, R., NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (2010) FEBS J., 277, pp. 4229-4240
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214
Pace, C.N., Vajdos, F., Fee, L., Grimsley, G., Gray, T., How to measure and predict the molar absorption coefficient of a protein (1995) Protein Sci., 4, pp. 2411-2423
Stan Tsai, C., (2007) Biomacromolecules: Introduction to Structure, Function and Informatics, pp. 183-209. , J. Wiley and sons, Hoboken, NJ
Marion, D., Ikura, M., Tschudin, R., Bax, A., Rapid recording of 2D NMR spectra without phase-cycling. Application to the study of hydrogen exchange in proteins (1989) J. Magn. Reson., 85, pp. 393-399
Piotto, M., Saudek, V., Sklenar, V., Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions (1992) J. Biomol. NMR, 2, pp. 661-665
Sklenar, V., Piotto, M., Leppik, R., Saudek, V., Gradient-tailored water suppression for 1H-15N HSQC experiments optimized to retain full sensitivity (1993) J. Magn. Reson. A, 102, pp. 241-245
Braunschweiler, L., Ernst, R.R., Coherence transfer by isotropic mixing: Application to proton correlation spectroscopy (1983) J. Magn. Reson., 53, pp. 521-528
Anil-Kumar, R.R., Wüthrich Ernst, K., A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules (1980) Biochem. Biophys. Res. Commun., 95, pp. 1-6
Hwang, T.L., Shaka, A.J., Water suppression that works: Excitation sculpting using arbitrary wave-forms and pulsed-field gradients (1995) J. Magn. Reson. A, 112, pp. 275-279
Dalvit, C., Efficient multiple-solvent suppression for the study of the interactions of organic solvents with biomolecules (1998) Journal of Biomolecular NMR, 11 (4), pp. 437-444
(2012) ACD/NMR Processor Freeware, , www.acdlabs.com, Version 12.01, Advanced Chemistry Development, Inc., Toronto, ON, Canada
Keller, R.L.J., (2004) The Computer Aided Resonance Assignment Tutorial, , CANTINA Verlag
Gunasekaran, K., Ma, B., Nussinov, R., Is allostery an intrinsic property of all dynamic proteins? (2004) Proteins: Structure, Function and Genetics, 57 (3), pp. 433-443. , DOI 10.1002/prot.20232
Niu, X., Chen, Q., Zhang, J., Shen, W., Shi, Y., Wu, J., Interesting structural and dynamical behaviors exhibited by the AF-6 PDZ domain upon Bcr peptide binding (2007) Biochemistry, 46 (51), pp. 15042-15053. , DOI 10.1021/bi701303p
Twomey, E.C., Cordasco, D.F., Wei, Y., Profound conformational changes of PED/PEA-15 in ERK2 complex revealed by NMR backbone dynamics (2012) BBA Prot. and Proteomics, 1824, pp. 1382-1393
Kersse, K., Verspurten, J., VandenBerghe, T., Vandenabeele, P., The death-fold superfamily of homotypic interaction motifs (2011) Trends Biochem. Sci., 36, pp. 541-552
Li, F.Y., Jeffrey, P.D., Yu, J.W., Shi, Y., Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition (2006) J. Biol. Chem., 281, pp. 2960-2968
Yang, J.K., Wang, L., Zheng, L., Wan, F., Ahmed, M., Lenardo, M.J., Wu, H., Crystal structure of MC159 reveals molecular mechanism of DISC assembly and FLIP inhibition (2005) Molecular Cell, 20 (6), pp. 939-949. , DOI 10.1016/j.molcel.2005.10.023, PII S1097276505017181
Bagneris, C., Ageichik, A.V., Cronin, N., Wallace, B., Collins, M., Boshoff, C., Waksman, G., Barrett, T., Crystal Structure of a vFlip-IKKgamma Complex: Insights into Viral Activation of the IKK Signalosome (2008) Molecular Cell, 30 (5), pp. 620-631. , DOI 10.1016/j.molcel.2008.04.029, PII S1097276508003602
Qin, H., Srinivasula, S.M., Wu, G., Fernandes-Alnemri, T., Alnemri, E.S., Shi, Y., Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1 (1999) Nature, 399, pp. 547-555
Srimathi, T., Robbins, S.L., Dubas, R.L., Chang, H., Cheng, H., Roder, H., Park, Y.C., Mapping of POP1-binding site on pyrin domain of ASC (2008) J. Biol. Chem., 283, pp. 15390-15398
Kam, Y., Exton, J.H., Dimerization of phospholipase D isozymes (2002) Biochemical and Biophysical Research Communications, 290 (1), pp. 375-380. , DOI 10.1006/bbrc.2001.6146
Koradi, R., Billeter, M., Wuthrich, K., MOLMOL: A program for display and analysis of macromolecular structures (1996) Journal of Molecular Graphics, 14 (1), pp. 51-55. , DOI 10.1016/0263-7855(96)00009-4
Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy
PED/PEA15 is a small protein involved in many protein-protein interactions that modulates the function of a number of key cellular effectors involved in major cell functions, including apoptosis, proliferation and glucose metabolism. In particular, PED/PEA15 interacts with the phospholipase D (PLD) isoforms 1 and 2 increasing protein kinase C-alpha isoform activity and affects both insulin-stimulated glucose transport and glucose-stimulated insulin secretion. The C-terminal portion (residues 712-1074) of PLD1, named D4, is still able to interact with PED/PEA15. In this study we characterized, by means of NMR spectroscopy, the molecular interaction of PED/PEA15 with D4 alpha, a smaller region of D4, encompassing residues 712-818, shown to have the same affinity for PED/PEA15 and to induce the same effects as D4 in PED/PEA15-overexpressing cells. Chemical shift perturbation (CSP) studies allowed to define D4 alpha binding site of PED/PEA15 and to identify a smaller region likely affected by an allosteric effect. Moreover, ELISA-like experiments showed that three 20-mer overlapping synthetic peptides, covering the 762-801 region of D4 alpha, strongly inhibit PED/PEA15-D4 alpha interaction through their binding to PED/PEA15 with K(D)s in low micromolar range. Finally, molecular details of the interaction of PED/PEA15 with one of the three peptides have been revealed by CSP and saturation transfer difference (STD) analyses. (C) 2013 Elsevier B.V. All rights reserved.
PED/PEA15 is a small protein involved in many protein-protein interactions that modulates the function of a number of key cellular effectors involved in major cell functions, including apoptosis, proliferation and glucose metabolism. In particular, PED/PEA15 interacts with the phospholipase D (PLD) isoforms 1 and 2 increasing protein kinase C-alpha isoform activity and affects both insulin-stimulated glucose transport and glucose-stimulated insulin secretion. The C-terminal portion (residues 712-1074) of PLD1, named D4, is still able to interact with PED/PEA15. In this study we characterized, by means of NMR spectroscopy, the molecular interaction of PED/PEA15 with D4 alpha, a smaller region of D4, encompassing residues 712-818, shown to have the same affinity for PED/PEA15 and to induce the same effects as D4 in PED/PEA15-overexpressing cells. Chemical shift perturbation (CSP) studies allowed to define D4 alpha binding site of PED/PEA15 and to identify a smaller region likely affected by an allosteric effect. Moreover, ELISA-like experiments showed that three 20-mer overlapping synthetic peptides, covering the 762-801 region of D4 alpha, strongly inhibit PED/PEA15-D4 alpha interaction through their binding to PED/PEA15 with K(D)s in low micromolar range. Finally, molecular details of the interaction of PED/PEA15 with one of the three peptides have been revealed by CSP and saturation transfer difference (STD) analyses. (C) 2013 Elsevier B.V. All rights reserved.
Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy
Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy
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Sacchi S, Cappelletti P, Pirone L, Smaldone G, Pedone E, Pollegioni L
* Elucidating the role of the pLG72 R30K substitution in schizophrenia susceptibility (358 views)
Febs Lett (ISSN: 1873-3468, 0014-5793, 0014-5793print), 2017 Feb; 591(4): 646-655.
Impact Factor: 2.999
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Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
* Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions (279 views) (PDF 172 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2016 Dec 05; 22(49): 17767-17775.
Impact Factor: 5.317
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Pirone L, Smaldone G, Esposito C, Balasco N, Petoukhov MV, Spilotros A, Svergun DI, Di Gaetano S, Vitagliano L, Pedone E
* Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners (323 views) (PDF 192 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016 Sep 24; 131: 106-114.
Impact Factor: 3.112
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Birolo L, Sacchi S, Smaldone G, Molla G, Leo G, Caldinelli L, Pirone L, Eliometri P, Di Gaetano S, Orefice I, Pedone E, Pucci P, Pollegioni L
* Regulating levels of the neuromodulator d-serine in human brain: structural insight into pLG72 and d-amino acid oxidase interaction (261 views) (PDF 207 views)
Febs J (ISSN: 1742-464x), 2016 Sep; 283(18): 3353-3370.
Impact Factor: 3.902
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Ruvo M, Doti N, Lorenzo V, Vitagliano L, Mascanzoni F
* The Interacting Domains of PREP1 and p160 are Endowed with a Remarkable Structural Stability (280 views)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2016 May; 58(5): 328-339.
Impact Factor: 1.634
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Bottini A, Wu B, Barile E, De SK, Leone M, Pellecchia M
* High-Throughput Screening (HTS) by NMR Guided Identification of Novel Agents Targeting the Protein Docking Domain of YopH (422 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7179linking), 2015 Nov 23; 11(8): 919-927.
Impact Factor: 2.98
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Correale S, de Paola I, Morgillo CM, Federico A, Zaccaro L, Pallante P, Galeone A, Fusco A, Pedone E, Luque FJ, Catalanotti B
* Structural Model of the hUbA1-UbcH10 Quaternary Complex: In Silico and Experimental Analysis of the Protein-Protein Interactions between E1, E2 and Ubiquitin (434 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2014 Nov 6; 9(11): e112082-e112082.
Impact Factor: 3.234
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Balasco N, Pirone L, Smaldone G, Di Gaetano S, Esposito L, Pedone E, Vitagliano L
* Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations (366 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014 Jul; 1844(7): 1289-1298.
Impact Factor: 4.882
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Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone E, Fattorusso R
* Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates (389 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2013 Sep 9; 19(37): 12217-12220.
Impact Factor: 5.696
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Attanasio F, Convertino M, Magno A, Caflisch A, Corazza A, Haridas H, Esposito G, Cataldo S, Pignataro B, Milardi D, Rizzarelli E
* Carnosine Inhibits Abeta(42) Aggregation By Perturbing The H-Bond Network In And Around The Central Hydrophobic Cluster (697 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2013 Mar 18; 14(5): 583-592.
Impact Factor: 3.06
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (414 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (357 views)
Chembiochem (ISSN: 1439-4227, 1439-9422, 1439-7633), 2013 Jan 2; 14(1): 100-106.
Impact Factor: 2.92
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Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
* Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its Interaction With Nucleic Acids And Nucleophosmin (npm1) (495 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2013; 452(3): 545-557.
Impact Factor: 4.779
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Mercurio FA, Marasco D, Pirone L, Pedone E, Pellecchia M, Leone M
* Solution Structure of the First Sam Domain of Odin and Binding Studies with the EphA2 Receptor (465 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2012 Mar 13; 51(10): 2136-2145.
Impact Factor: 3.377
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Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (390 views)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
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Ruvo M, Sandomenico A, Tudisco L, De Falco S
* Branched peptides for the modulation of protein-protein interactions: More arms are better than one? (523 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2011 Jun; 18(16): 2429-2437.
Impact Factor: 4.859
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Correale S, Pirone L, Di Marcotullio L, De Smaele E, Greco A, Mazzà D, Moretti M, Alterio V, Vitagliano L, Di Gaetano S, Gulino A, Pedone E
* Molecular organization of the cullin E3 ligase adaptor KCTD11 (435 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Apr; 93(4): 715-724.
Impact Factor: 3.022
View Export to BibTeX Export to EndNote
Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (674 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
View Export to BibTeX Export to EndNote
Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (558 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Molecular dynamics studies of the P pilus rod subunit PapA (485 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 192-199.
Impact Factor: 1.807
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Merlino A, Varriale S, Coscia MR, Mazzarella L, Oreste U
* Structure and dimerization of the teleost transmembrane immunoglobulin region (362 views)
Journal Of Molecular Graphics & Modelling (ISSN: 1093-3263), 2008 Oct; 27(3): 401-407.
Impact Factor: 2.347
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Conformational states and association mechanism of Yersinia pestis Caf1 subunits (322 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2008 Sep 8; 372(4): 804-810.
Impact Factor: 2.648
View Export to BibTeX Export to EndNote
Ruvo M, Marasco D, Viparelli F, Tornatore L, Sandomenico A, Doti N, Pedone C, Benedetti E, Monti SM
* Peptide antagonists of protein-protein interactions identified by screening protein fragments derived by enzyme degradation (349 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008 Sep; 14(8): N/D-N/D.
Impact Factor: 1.654
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (445 views)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
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Tornatore L, Marasco D, Dathan N, Vitale RM, Benedetti E, Papa S, Franzoso G, Ruvo M, Monti SM
* Gadd45 beta forms a homodimeric complex that binds tightly to MKK7 (467 views)
J Mol Biol (ISSN: 0022-2836, 0022-2283, 1089-8638), 2008 Apr 18; 378(1): 97-111.
Impact Factor: 5.673
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* A Molecular Dynamics Study of Pilus Subunits: Insights into Pilus Biogenesis (447 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2007 Apr 6; 367(4): 935-941.
Impact Factor: 4.472
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* Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA-mimic protein and may be involved in the adaptation of Burkholderia cenocepacia (5 views)
Microbiologyopen (ISSN: 2045-8827linking), 2022 Feb; 11(1): e1264-N/D.
Impact Factor: 3.48
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Vincenzi M, Mercurio FA, Leone M
* Protein Interaction Domains: structural features and drug discovery applications (part 2) (79 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2021; 28(5): 854-892.
Impact Factor: 4.184
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Tornatore L, Capece D, Sandomenico A, Verzella D, Vecchiotti D, Zazzeroni F, Ruvo M, Franzoso G
* The Screening of Combinatorial Peptide Libraries for Targeting Key Molecules or Protein-Protein Interactions in the NF-κB Pathway (8 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2366: 343-356.
Impact Factor: 1.17
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Mercurio FA, Di Natale C, Pirone L, Vincenzi M, Marasco D, De Luca S, Pedone EM, Leone M
* Exploring the ability of cyclic peptides to target SAM domains: a computational and experimental study (266 views)
Chembiochem (ISSN: 1439-4227linking, 1439-7633), 2020 Mar 2; 21(5): 702-711.
Impact Factor: 2.641
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Vincenzi M, Mercurio FA, Leone M
* Sam Domains in Multiple Diseases (237 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2020; 27(3): 450-476.
Impact Factor: 3.469
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Rega C, Russo R, Foca A, Sandomenico A, Iaccarino E, Raimondo D, Milanetti E, Tornatore L, Franzoso G, Pedone PV, Ruvo M, Chambery A
* Probing the interaction interface of the GADD45beta/MKK7 and MKK7/DTP3 complexes by chemical cross-linking mass spectrometry (276 views)
Int J Biol Macromol (ISSN: 1879-0003electronic, 0141-8130linking), 2018 Jul 15; 114: 114-123.
Impact Factor: 1.427
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Pirone L, Ripoll-rozada J, Leone M, Ronca R, Lombardo F, Fiorentino G, Andersen JF, Pereira PJB, Arcà B, Pedone E
* Functional analyses yield detailedinsight into the mechanism of thrombin inhibition by the antihemostatic salivary protein cE5 from Anopheles gambiae (367 views) (PDF 190 views)
Jbc Papers (ISSN: 0021-9258linking, 1083-351x), 2017 Jul 28; 292(30): 12632-12642.
Impact Factor: 4.125
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Sacchi S, Cappelletti P, Pirone L, Smaldone G, Pedone E, Pollegioni L
* Elucidating the role of the pLG72 R30K substitution in schizophrenia susceptibility (358 views)
Febs Lett (ISSN: 1873-3468, 0014-5793, 0014-5793print), 2017 Feb; 591(4): 646-655.
Impact Factor: 2.999
View Export to BibTeX Export to EndNote
Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
* Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions (279 views) (PDF 172 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2016 Dec 05; 22(49): 17767-17775.
Impact Factor: 5.317
View Export to BibTeX Export to EndNote
Pirone L, Smaldone G, Esposito C, Balasco N, Petoukhov MV, Spilotros A, Svergun DI, Di Gaetano S, Vitagliano L, Pedone E
* Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners (323 views) (PDF 192 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016 Sep 24; 131: 106-114.
Impact Factor: 3.112
View Export to BibTeX Export to EndNote
Birolo L, Sacchi S, Smaldone G, Molla G, Leo G, Caldinelli L, Pirone L, Eliometri P, Di Gaetano S, Orefice I, Pedone E, Pucci P, Pollegioni L
* Regulating levels of the neuromodulator d-serine in human brain: structural insight into pLG72 and d-amino acid oxidase interaction (261 views) (PDF 207 views)
Febs J (ISSN: 1742-464x), 2016 Sep; 283(18): 3353-3370.
Impact Factor: 3.902
View Export to BibTeX Export to EndNote
Ruvo M, Doti N, Lorenzo V, Vitagliano L, Mascanzoni F
* The Interacting Domains of PREP1 and p160 are Endowed with a Remarkable Structural Stability (280 views)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2016 May; 58(5): 328-339.
Impact Factor: 1.634
View Export to BibTeX Export to EndNote
Bottini A, Wu B, Barile E, De SK, Leone M, Pellecchia M
* High-Throughput Screening (HTS) by NMR Guided Identification of Novel Agents Targeting the Protein Docking Domain of YopH (422 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7179linking), 2015 Nov 23; 11(8): 919-927.
Impact Factor: 2.98
View Export to BibTeX Export to EndNote
Correale S, de Paola I, Morgillo CM, Federico A, Zaccaro L, Pallante P, Galeone A, Fusco A, Pedone E, Luque FJ, Catalanotti B
* Structural Model of the hUbA1-UbcH10 Quaternary Complex: In Silico and Experimental Analysis of the Protein-Protein Interactions between E1, E2 and Ubiquitin (434 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2014 Nov 6; 9(11): e112082-e112082.
Impact Factor: 3.234
View Export to BibTeX Export to EndNote
Balasco N, Pirone L, Smaldone G, Di Gaetano S, Esposito L, Pedone E, Vitagliano L
* Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations (366 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014 Jul; 1844(7): 1289-1298.
Impact Factor: 4.882
View Export to BibTeX Export to EndNote
Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone E, Fattorusso R
* Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates (389 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2013 Sep 9; 19(37): 12217-12220.
Impact Factor: 5.696
View Export to BibTeX Export to EndNote
Attanasio F, Convertino M, Magno A, Caflisch A, Corazza A, Haridas H, Esposito G, Cataldo S, Pignataro B, Milardi D, Rizzarelli E
* Carnosine Inhibits Abeta(42) Aggregation By Perturbing The H-Bond Network In And Around The Central Hydrophobic Cluster (697 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2013 Mar 18; 14(5): 583-592.
Impact Factor: 3.06
View Export to BibTeX Export to EndNote
Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (414 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
View Export to BibTeX Export to EndNote
Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (357 views)
Chembiochem (ISSN: 1439-4227, 1439-9422, 1439-7633), 2013 Jan 2; 14(1): 100-106.
Impact Factor: 2.92
View Export to BibTeX Export to EndNote
Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
* Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its Interaction With Nucleic Acids And Nucleophosmin (npm1) (495 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2013; 452(3): 545-557.
Impact Factor: 4.779
View Export to BibTeX Export to EndNote
Mercurio FA, Marasco D, Pirone L, Pedone E, Pellecchia M, Leone M
* Solution Structure of the First Sam Domain of Odin and Binding Studies with the EphA2 Receptor (465 views)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2012 Mar 13; 51(10): 2136-2145.
Impact Factor: 3.377
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (390 views)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
View Export to BibTeX Export to EndNote
Ruvo M, Sandomenico A, Tudisco L, De Falco S
* Branched peptides for the modulation of protein-protein interactions: More arms are better than one? (523 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2011 Jun; 18(16): 2429-2437.
Impact Factor: 4.859
View Export to BibTeX Export to EndNote
Correale S, Pirone L, Di Marcotullio L, De Smaele E, Greco A, Mazzà D, Moretti M, Alterio V, Vitagliano L, Di Gaetano S, Gulino A, Pedone E
* Molecular organization of the cullin E3 ligase adaptor KCTD11 (435 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Apr; 93(4): 715-724.
Impact Factor: 3.022
View Export to BibTeX Export to EndNote
Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (674 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
View Export to BibTeX Export to EndNote
Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (558 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Molecular dynamics studies of the P pilus rod subunit PapA (485 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 192-199.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Merlino A, Varriale S, Coscia MR, Mazzarella L, Oreste U
* Structure and dimerization of the teleost transmembrane immunoglobulin region (362 views)
Journal Of Molecular Graphics & Modelling (ISSN: 1093-3263), 2008 Oct; 27(3): 401-407.
Impact Factor: 2.347
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Conformational states and association mechanism of Yersinia pestis Caf1 subunits (322 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2008 Sep 8; 372(4): 804-810.
Impact Factor: 2.648
View Export to BibTeX Export to EndNote
Ruvo M, Marasco D, Viparelli F, Tornatore L, Sandomenico A, Doti N, Pedone C, Benedetti E, Monti SM
* Peptide antagonists of protein-protein interactions identified by screening protein fragments derived by enzyme degradation (349 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008 Sep; 14(8): N/D-N/D.
Impact Factor: 1.654
View Export to BibTeX Export to EndNote
Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (445 views)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Impact Factor: 1.621
View Export to BibTeX Export to EndNote
Tornatore L, Marasco D, Dathan N, Vitale RM, Benedetti E, Papa S, Franzoso G, Ruvo M, Monti SM
* Gadd45 beta forms a homodimeric complex that binds tightly to MKK7 (467 views)
J Mol Biol (ISSN: 0022-2836, 0022-2283, 1089-8638), 2008 Apr 18; 378(1): 97-111.
Impact Factor: 5.673
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* A Molecular Dynamics Study of Pilus Subunits: Insights into Pilus Biogenesis (447 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2007 Apr 6; 367(4): 935-941.
Impact Factor: 4.472
View Export to BibTeX Export to EndNote
33 Records (30 excluding Abstracts).
Total impact factor: 107.753 (97.192 excluding Abstracts).
Total 5 year impact factor: 120.472 (110.255 excluding Abstracts).
Total impact factor: 107.753 (97.192 excluding Abstracts).
Total 5 year impact factor: 120.472 (110.255 excluding Abstracts).
467 views. Last view on Sunday 19 March 2023, 8:57:18
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