Human Carbonic Anhydrase Vii Protects Cells From Oxidative Damage(367 views) Del Giudice R, Monti DM, Truppo E, Arciello A, Supuran CT, De Simone G, Monti SM
Keywords: Carbonic Anhydrase, Eukaryotic Cells, Oxidative Stress Protection, S-Glutathionylation, Actin, Carbonate Dehydratase, Carbonate Dehydratase Vii, Cysteine, Procaspase 3, Protein Bax, Protein Bcl 2, Serine, Unclassified Drug, Apoptosis, Article, Cell Damage, Cell Protection, Cell Viability, Dna Sequence, Expression Vector, Genetic Transfection, Human, Human Cell, Immunofluorescence, Priority Journal, Signal Transduction, Western Blotting, Wild Type, Fluorescent Antibody Technique, Hela Cells,
Affiliations: *** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, I-80126 Naples, Italy
Institute of Biostructures and Bioimaging-CNR, Via Mezzocannone 16, I-80134 Naples, Italy
Department of NEUROFARBA, University of Florence, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Florence, Italy
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Human Carbonic Anhydrase Vii Protects Cells From Oxidative Damage
Human carbonic anhydrase (hCA) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Recently, S-glutathionylation of two cysteine residues from the enzyme was revealed, suggesting a new role as oxygen radical scavenger. We analyzed the effect of native and tetramutated hCA VII (all cysteines mutated into serines) in a eukaryotic system by stressing cells with an oxidant agent. Results clearly show that native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process. Our findings definitively confirm hCA VII protective role toward oxidative insult
Human Carbonic Anhydrase Vii Protects Cells From Oxidative Damage