Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries (366 views)
Am J Physiol Heart Circ Physiol American Journal Of Physiology Heart And Circulatory Physiology (ISSN: 0363-6135), 2003; 285(154-1): H369-H369.
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Paper type: Journal Article,
Impact factor: 3.658, 5-year impact factor: 4.046
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-17644434970&partnerID=40&md5=7be520972b84b443a30cc041dbcb19b7
Keywords: Aging, Beta-Adrenergic Receptor, Exercise, Acetylcholine, Alpha 2 Adrenergic Receptor Stimulating Agent, Beta Adrenergic Receptor Stimulating Agent, Brimonidine, G Protein Coupled Receptor Kinase, Isoprenaline, Muscarinic Agent, N(g) Methylarginine, Nitroprusside Sodium, Phenylephrine, Adrenergic System, Animal Experiment, Article, Blood Vessel Reactivity, Carotid Artery, Controlled Study, Desensitization, Enzyme Activity, Nonhuman, Priority Journal, Receptor Down Regulation, Signal Transduction, Vasodilatation, Adrenergic Beta-Agonists, Blotting, Western, Body Weight, Carotid Arteries, Cells, Cultured, Cytosol, Endothelium, Vascular, Enzyme Inhibitors, Isoproterenol, Muscle Relaxation, Smooth, Nitric Oxide Synthase, Nitric Oxide Synthase Type Iii, Omega-N-Methylarginine, Organ Size, Physical Conditioning, Quinoxalines, Inbred Wky, Vasodilator Agents,
Affiliations:
*** IBB - CNR ***
Department of Geriatrics, University of Naples Federico II, 80131 Naples, Italy
Department of Internal Medicine, University of Naples Federico II, 80131 Naples, Italy
Department of Gerontology, II University of Naples, 80131 Naples, Italy
Cattedra di Geriatria, Univ. degli Studi Napoli Federico II, Via Sergio Pansini 5, 80131 Naples, Italy
References:
Not available.
Am J Physiol Heart Circ Physiol American Journal Of Physiology Heart And Circulatory Physiology (ISSN: 0363-6135), 2003; 285(154-1): H369-H369.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.658, 5-year impact factor: 4.046
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-17644434970&partnerID=40&md5=7be520972b84b443a30cc041dbcb19b7
Keywords: Aging, Beta-Adrenergic Receptor, Exercise, Acetylcholine, Alpha 2 Adrenergic Receptor Stimulating Agent, Beta Adrenergic Receptor Stimulating Agent, Brimonidine, G Protein Coupled Receptor Kinase, Isoprenaline, Muscarinic Agent, N(g) Methylarginine, Nitroprusside Sodium, Phenylephrine, Adrenergic System, Animal Experiment, Article, Blood Vessel Reactivity, Carotid Artery, Controlled Study, Desensitization, Enzyme Activity, Nonhuman, Priority Journal, Receptor Down Regulation, Signal Transduction, Vasodilatation, Adrenergic Beta-Agonists, Blotting, Western, Body Weight, Carotid Arteries, Cells, Cultured, Cytosol, Endothelium, Vascular, Enzyme Inhibitors, Isoproterenol, Muscle Relaxation, Smooth, Nitric Oxide Synthase, Nitric Oxide Synthase Type Iii, Omega-N-Methylarginine, Organ Size, Physical Conditioning, Quinoxalines, Inbred Wky, Vasodilator Agents,
Affiliations:
*** IBB - CNR ***
Department of Geriatrics, University of Naples Federico II, 80131 Naples, Italy
Department of Internal Medicine, University of Naples Federico II, 80131 Naples, Italy
Department of Gerontology, II University of Naples, 80131 Naples, Italy
Cattedra di Geriatria, Univ. degli Studi Napoli Federico II, Via Sergio Pansini 5, 80131 Naples, Italy
References:
Not available.
Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries
Aging is associated with alterations in β-adrenergic receptor (β-AR) signaling and reduction in cardiovascular responses to β-AR stimulation. Because exercise can attenuate age-related impairment in myocardial β-AR signaling and function, we tested whether training could also exert favorable effects on vascular β-AR responses. We evaluated common carotid artery responsiveness in isolated vessel ring preparations from 8 aged male Wistar-Kyoto (WKY) rats trained for 6 wk in a 5 days/wk swimming protocol, 10 untrained agematched rats, and 10 young WKY rats. Vessels were preconstricted with phenylephrine (10-6 M), and vasodilation was assessed in response to the β-AR agonist isoproterenol (10-10-3 × 10-8 M), the α2-AR agonist UK-14304 (10-9-10-6 M), the muscarinic receptor agonist ACh (10-9-10-6 M), and nitroprusside (10-8-10-5 M). β-AR density and cytoplasmic β-AR kinase (β-ARK) activity were tested on pooled carotid arteries. β-ARK expression was assessed in two endothelial cell lines from bovine aorta and aorta isolated from a 12-wk WKY rat. β-AR, α2-AR, and muscarinic responses, but not that to nitroprusside, were depressed in untrained aged vs. young animals. Exercise training restored β-AR and muscarinic responses but did not affect vasodilation induced by UK-14304 and nitroprusside. Aged carotid arteries showed reduced β-AR number and increased β-ARK activity. Training counterbalanced these phenomena and restored β-AR density and β-ARK activity to levels observed in young rat carotids. Our data indicate that age impairs β-AR vasorelaxation in rat carotid arteries through β-AR downregulation and desensitization. Exercise restores this response and reverts age-related modification in β-ARs and β-ARK. Our data support an important role for β-ARK in vascular β-AR vasorelaxation.
Aging is associated with alterations in β-adrenergic receptor (β-AR) signaling and reduction in cardiovascular responses to β-AR stimulation. Because exercise can attenuate age-related impairment in myocardial β-AR signaling and function, we tested whether training could also exert favorable effects on vascular β-AR responses. We evaluated common carotid artery responsiveness in isolated vessel ring preparations from 8 aged male Wistar-Kyoto (WKY) rats trained for 6 wk in a 5 days/wk swimming protocol, 10 untrained agematched rats, and 10 young WKY rats. Vessels were preconstricted with phenylephrine (10-6 M), and vasodilation was assessed in response to the β-AR agonist isoproterenol (10-10-3 × 10-8 M), the α2-AR agonist UK-14304 (10-9-10-6 M), the muscarinic receptor agonist ACh (10-9-10-6 M), and nitroprusside (10-8-10-5 M). β-AR density and cytoplasmic β-AR kinase (β-ARK) activity were tested on pooled carotid arteries. β-ARK expression was assessed in two endothelial cell lines from bovine aorta and aorta isolated from a 12-wk WKY rat. β-AR, α2-AR, and muscarinic responses, but not that to nitroprusside, were depressed in untrained aged vs. young animals. Exercise training restored β-AR and muscarinic responses but did not affect vasodilation induced by UK-14304 and nitroprusside. Aged carotid arteries showed reduced β-AR number and increased β-ARK activity. Training counterbalanced these phenomena and restored β-AR density and β-ARK activity to levels observed in young rat carotids. Our data indicate that age impairs β-AR vasorelaxation in rat carotid arteries through β-AR downregulation and desensitization. Exercise restores this response and reverts age-related modification in β-ARs and β-ARK. Our data support an important role for β-ARK in vascular β-AR vasorelaxation.
Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries
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Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries
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Ruggiero D, Paolillo S, Ratta GD, Mariniello A, Formisano T, Pellegrino AM, Filardi PP
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Monaldi Arch Chest Dis Card Ser (ISSN: 1122-0643, 1122-0643print), 2013 Sep; 80(3): 106-110.
Impact Factor: 7.132
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Iaccarino G, Ciccarelli M, Sorriento D, Cipolletta E, Cerullo V, Iovino GL, Paudice A, Elia A, Santulli G, Campanile A, Arcucci O, Pastore L, Salvatore F, Condorelli G, Trimarco B
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Circulationcirculation (ISSN: 0009-7322), 2004; 109(21): 2587-2593.
Impact Factor: 12.563
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Blomqvist G, Tavitian B, Pappata S, Crouzel C, Jobert A, Doignon I, Di Giamberardino L
* Quantitative measurement of cerebral acetylcholinesterase using (250 views)
Journal Of Cerebral Blood Flow And Metabolism (ISSN: 0271-678x), 2001 Feb; 21(2): 114-131.
Impact Factor: 5.477
View Export to BibTeX Export to EndNote
* [Endothelial function as a marker of pre-clinical atherosclerosis: assessment techniques and clinical implications] (351 views)
Monaldi Arch Chest Dis Card Ser (ISSN: 1122-0643, 1122-0643print), 2013 Sep; 80(3): 106-110.
Impact Factor: 7.132
View Export to BibTeX Export to EndNote
Iaccarino G, Ciccarelli M, Sorriento D, Cipolletta E, Cerullo V, Iovino GL, Paudice A, Elia A, Santulli G, Campanile A, Arcucci O, Pastore L, Salvatore F, Condorelli G, Trimarco B
* AKT participates in endothelial dysfunction in hypertension (375 views)
Circulationcirculation (ISSN: 0009-7322), 2004; 109(21): 2587-2593.
Impact Factor: 12.563
View Export to BibTeX Export to EndNote
Blomqvist G, Tavitian B, Pappata S, Crouzel C, Jobert A, Doignon I, Di Giamberardino L
* Quantitative measurement of cerebral acetylcholinesterase using (250 views)
Journal Of Cerebral Blood Flow And Metabolism (ISSN: 0271-678x), 2001 Feb; 21(2): 114-131.
Impact Factor: 5.477
View Export to BibTeX Export to EndNote
3 Records (3 excluding Abstracts).
Total impact factor: 25.172 (25.172 excluding Abstracts).
Total 5 year impact factor: 27.615 (27.615 excluding Abstracts).
Total impact factor: 25.172 (25.172 excluding Abstracts).
Total 5 year impact factor: 27.615 (27.615 excluding Abstracts).
366 views. Last view on Friday 24 November 2023, 1:19:23
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