AN INTERIM ANALYSIS ON SUSCEPTIBILITY TO VASCULAR ALTERATIONS IN MS AND ALS(466 views) Postiglione E, Criscuolo C, Cianflone A, Lanzillo R, Mancini M, Tedeschi E, Liuzzi R, Vacca G, Vastola M, Capasso M, Mancino T, Mormile R, Passannanti A, Palma G, Caprio M, Incoronato M, Salvatore M, Brescia-Morra V
Veins And Lymphatics (ISSN: 2279-7483), 2015; 4(s1): 21-22.
1Neurosciences, Reproductive and Odontostomatological Sciences,University Federico II, Naples; 2Institute of Biostructure and Bioimaging, National Research Council, Naples; 3Advanced Biomedical Sciences, University Federico II, Naples; 4Haemostasis and Thrombosis Laboratory, DAI of Laboratory Medicine, University Federico II, Naples; 5IRCSS SDN Foundation, Naples, Italy
ackground: Among the factors contributing to brain damage in Multiple Sclerosis (MS), scientific evidences indicate ischemic changes, venous outflow abnormalities and accumulation of proinflammatory and neurotoxic substances.
Objectives
: To study the association among MS, amyotrophic lateral sclerosis (ALS), and vascular changes at molecular, genetic, anatomic and functional level.
Methods
: 300 MS patients, 50 ALS patients, and 300 healthy subjects (HS) will be recruited over 3 years. To assess the endothelial dysfunction development risk and/or a genetic susceptibility, serum levels and single nucleotide polymorphisms (SNP) of homocysteine,VEGF-A, Endothelin 1 (ET-1) and HIF1A will be analyzed and correlated
to micro and macro vascular abnormalities detected by Magnetic Resonance (MR) and Ultrasound (US) imaging.
Results
: Homocysteine levels (HL) were performed in 156 MS, 15 ALS patients and 145 HS, VEGF-A in 50 patients vs 25 HS. Median HL were 13.4 μmol/L in MS, 12.82 μmol/L in ALS patients and 12.6 μmol/L in HS. At Kruskal-Wallis test median HL values were significantly different in the three groups (P=0.002). In particular, median HL were significantly higher in MS and ALS patients vs HS (P<0.05). In MS women median HL were significantly higher compared
to HS (12 vs 9.94 μmol/L, P<0.00001), furthermore HL correlated to age in MS females. Median VEGF-A values tended to be higher in MS patients vs HS (9.68 vs 0, P=0.196). 101 patients (78 RR, 2 PP, 11 SP and 10 ALS) underwent contrast-enhanced brain MR, and 68 patients (54 RR, 1 PP, 10 SP and 3 ALS) underwent US evaluation.
References: Not available.
AN INTERIM ANALYSIS ON SUSCEPTIBILITY TO VASCULAR ALTERATIONS IN MS AND ALS
Background:
Among the factors contributing to brain damage in Multiple Sclerosis (MS), scientific evidences indicate ischemic changes, venous outflow abnormalities and accumulation of proinflammatory and neurotoxic substances.
Objectives:
To study the association among MS, amyotrophic lateral sclerosis (ALS), and vascular changes at molecular, genetic, anatomic and functional level.
Methods:
300 MS patients, 50 ALS patients, and 300 healthy subjects (HS) will be recruited over 3 years. To assess the endothelial dysfunction development risk and/or a genetic susceptibility, serum levels and single nucleotide polymorphisms (SNP) of homocysteine, VEGF-A, Endothelin 1 (ET-1) and HIF1A will be analyzed and correlated
to micro and macro vascular abnormalities detected by Magnetic Resonance (MR) and Ultrasound (US) imaging.
Results
: Homocysteine levels (HL) were performed in 156 MS, 15 ALS patients and 145 HS, VEGF-A in 50 patients vs 25 HS. Median HL were 13.4 μmol/L in MS, 12.82 μmol/L in ALS patients and 12.6 μmol/L in HS. At Kruskal-Wallis test median HL values were significantly different in the three groups (P=0.002). In particular, median HL were significantly higher in MS and ALS patients vs HS (P<0.05). In MS women median HL were significantly higher compared
to HS (12 vs 9.94 μmol/L, P<0.00001), furthermore HL correlated to age in MS females. Median VEGF-A values tended to be higher in MS patients vs HS (9.68 vs 0, P=0.196). 101 patients (78 RR, 2 PP, 11 SP and 10 ALS) underwent contrast-enhanced brain MR, and 68 patients (54 RR, 1 PP, 10 SP and 3 ALS) underwent US evaluation.
Conclusions
: Combining different molecular analysis and imaging modalities may provide new insights into the vascular aspects of MS pathogenesis. These preliminary results support an altered vascular profile in MS, especially in females, and ALS patients. Definitive results will be available at project termination in 2016.
AN INTERIM ANALYSIS ON SUSCEPTIBILITY TO VASCULAR ALTERATIONS IN MS AND ALS