Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2016 Feb 13; 80: 590-600.
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Paper type: Journal Article,
Impact factor: 7.78, 5-year impact factor: 6.862
Url: https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84958279448&partnerID=40&md5=c96f2cbabc0f0d7d7978b2176151767d
Keywords: Biomolecular Sensing, Human Thyroglobulin (tg) Detection, Label-Free Detection, Long Period Fiber Grating (lpg), Optical Fiber Biosensor, Reflection-Type Lpg, Antibodies, Bioassay, Biomarkers, Biopsy, Diffraction Gratings, Fiber Optic Sensors, Glycoproteins, Needles, Reflection Type, Chemical Detection,
Affiliations:
*** IBB - CNR ***
Optoelectronics Group, Department of Engineering, University of Sannio, Benevento, Italy., Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche (IBB-CNR), Napoli, Italy; Centro Interuniversitario di Ricerca sui Peptidi Bioattivi (CIRPeB), Napoli, Italy., Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche (IBB-CNR), Napoli, Italy; Centro Interuniversitario di Ricerca sui Peptidi Bioattivi (CIRPeB), Napoli, Italy. Electronic address: menotti.ruvo@unina.it., Institute for Polymers, Composites and Biomaterials (IPCB) -CNR, Portici, Italy. Electronic address: anna.borriello@cnr.it., Department of Clinical Medicine and Surgery, University of Napoli "Federico II", Napoli, Italy., Department of Traslational Medicine, University of Napoli "Federico II", Napoli, Italy., Hospital Consulting SpA, Bagno a Ripoli, Firenze, Italy., Optoelectronics Group, Department of Engineering, University of Sannio, Benevento, Italy. Electronic address: a.cusano@unisannio.it.,
References:
Not available.
We report an innovative fiber optic nano-optrode based on Long Period Gratings (LPGs) working in reflection mode for the detection of human Thyroglobulin (TG), a protein marker of differentiated thyroid cancer.
The reflection-type LPG (RT-LPG) biosensor, coated with a single layer of atactic polystyrene (aPS) onto which a specific, high affinity anti-Tg antibody was adsorbed, allowed the label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels.
Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels. The system is characterized by a very high sensitivity and specificity allowing the
ex-vivo
detection of sub ng/ml concentrations of human Tg from needle washouts of fine-needle aspiration biopsies of thyroid nodule from different patients.
No results. |
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Anal Bioanal Chem (ISSN: 1618-2642), 2016; 408(3): 849-854.
Impact Factor: 3.431
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Pilla P, Sandomenico A, Malachovsk V, Borriello A, Giordano M, Cutolo A, Ruvo M, Cusano A
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Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic, 0956-5663linking), 2012 Jan 15; 31(1): 486-491.
Impact Factor: 5.437
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Total impact factor: 8.868 (8.868 excluding Abstracts).
Total 5 year impact factor: 8.695 (8.695 excluding Abstracts).
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