The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies (342 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2016; 1864(2): 195-203.
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Paper type: Journal Article,
Impact factor: 5.476, 5-year impact factor: 5.725
Url: https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84948453364&partnerID=40&md5=6569aec1c5036717080cf6d0ed6aa37a
Keywords: Autoprocessing, Single-Point Mutation, X-Ray Crystallography, γ-Glutamyltranspeptidase, γ-Gt Precursor, Alanine, Bacterial Enzyme, Carbonyl Derivative, Enzyme Precursor, Gamma Glutamyltransferase, Hydroxyl Group, Threonine, Article, Bacillus Licheniformis, Controlled Study, Crystal Structure, Enzyme Activity, Enzyme Mechanism, Enzyme Structure, Hydrogen Bond, Nonhuman, Nucleophilicity, Priority Journal, Site Directed Mutagenesis, X Ray Crystallography,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone, 16, Naples, Italy
Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi City, Taiwan
References:
Castellano, I., Merlino, A., (2012) Cell. Mol. Life Sci., 69, pp. 3381-339
Stole, E., Smith, T.K., Manning, J.M., Meister, A., (1994) J. Biol. Chem., 269, pp. 21435-21439
Tate, S.S., Meister, A., (1981) Mol. Cell. Biochem., p. 39
Hanigan, M.H., Ricketts, W.A., (1993) Biochemistry, 32, pp. 6302-6306
Taniguchi, N., Ikeda, Y., (1998) Adv. Enzymol. Relat. Areas Mol. Biol., p. 72
Hanigan, M.H., Gallagher, B.C., Townsend, D.M., Gabarra, V., (1999) Carcinogenesis, 20, pp. 553-559
Wilk, S., Mizoguchi, H., Orlowski, M., (1978) J. Pharmacol. Exp. Ther., 206, pp. 227-232
Zhang, Q., Kulczynska, A., Webb, D.J., Megson, I.L., Botting, N.P., (2013) Chem. Commun., 49, pp. 1389-1391
Allison, R.D., (1985) Methods Enzymol., 113, pp. 419-437
Tate, S.S., (1986) FEBS Lett., 194, pp. 33-38
Inoue, M., Hiratake, J., Suzuki, H., Kumagai, H., Sakata, K., (2000) Biochemistry, 39, pp. 7764-7771
Xu, Q., Buckley, D., Guan, C., Guo, H.-C., (1999) Cell, 98, pp. 651-661
Qian, X., Guan, C., Guo, H.-C., (2003) Structure, 11, pp. 997-1003
Kim, Y., Yoon, K.-H., Khang, Y., Turley, S., Hol, W.G., (2000) Structure, 8, pp. 1059-1068
Hewitt, L., Kasche, V., Lummer, K., Lewis, R., Murshudov, G., Verma, C., Dodson, G., Wilson, K., (2000) J. Mol. Biol., 302, pp. 887-898
Ditzel, L., Huber, R., Mann, K., Heinemeyer, W., Wolf, D.H., Groll, M., (1998) J. Mol. Biol., 279, pp. 1187-1191
Bokhove, M., Yoshida, H., Hensgens, C.M., Van Der Laan, J.M., Sutherland, J.D., Dijkstra, B.W., (2010) Structure, 18, pp. 301-308
Minami, H., Suzuki, H., Kumagai, H., (2003) FEMS Microbiol. Lett., 224, pp. 169-173
Boanca, G., Sand, A., Barycki, J.J., (2006) J. Biol. Chem., 281, pp. 19029-19037
Hung, C.-P., Yang, J.-C., Chen, J.-H., Chi, M.-C., Lin, L.-L., (2011) J. Biol. Phys., 37, pp. 463-475
Murty, N.A., Tiwary, E., Sharma, R., Nair, N., Gupta, R., (2012) Enzym. Microb. Technol., 50, pp. 159-164
Chang, H.P., Liang, W.C., Lyu, R.C., Chi, M.C., Wang, T.F., Su, K.L., Hung, H.C., Lin, L.L., (2010) Biochemistry (Mosc), 75, pp. 919-929
Okada, T., Suzuki, H., Wada, K., Kumagai, H., Fukuyama, K., (2006) Proc. Natl. Acad. Sci. U. S. A., 103, pp. 6471-6476
Wada, K., Irie, M., Suzuki, H., Fukuyama, K., (2010) FEBS J., 277, pp. 1000-1009
Oinonen, C., Rouvinen, J., (2000) Protein Sci., p. 9
Lee, Y.S., Kim, H.W., Lee, K.B., Park, S.S., (2000) Biochim. Biophys. Acta, 1523, pp. 123-127
Kim, Y., Kim, S., Earnest, T.N., Hol, W.G., (2002) J. Biol. Chem., 277, pp. 2823-2829
West, M.B., Chen, Y., Wickham, S., Heroux, A., Cahill, K., Hanigan, M.H., Mooers, B.H., (2013) J. Biol. Chem., 288, pp. 31902-31913
Lin, L.L., Chen, Y.Y., Chi, M.C., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 1523-1529
Moallic, C., Dabonne, S., Colas, B., Sine, J.P., (2006) Protein J., 25, pp. 391-397
Castellano, I., Di Salle, A., Merlino, A., Rossi, M., Laara, F., (2011) Extremophiles, 15, pp. 259-270
Chi, M.C., Lo, Y.H., Chen, Y.Y., Lin, L.L., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 2290-2297
Lyu, R.C., Hu, H.Y., Kuo, L.Y., Lo, H.F., Ong, P.L., Chang, H.P., Lin, L.L., (2009) Curr. Microbiol., 59, pp. 101-106
Otwinowski, Z., Minor, W., (1997) Methods in Enzymology, 276, pp. 307-326. , ed. J. Charles W. Carter, Academic Press
Emsley, P., Cowtan, K., (2004) Acta Crystallogr. Sect. D: Biol. Crystallogr., 60, pp. 2126-2132
Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., Echols, N., Headd, J.J., Zwart, P.H., (2010) Acta Crystallogr. Sect. D: Biol. Crystallogr., 66, pp. 213-221
Noren, C.J., Wang, J., Perler, F.B., (2000) Angew. Chem., 39, pp. 450-466
Chi, M.C., Chen, Y.Y., Lo, H.F., Lin, L.L., (2012) FEBS Open Bio, 2, pp. 298-304
Hashimoto, W., Suzuki, H., Nohara, S., Kumagai, H., (1992) Biochem. Biophys. Res. Commun., 189, pp. 173-178
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2016; 1864(2): 195-203.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 5.476, 5-year impact factor: 5.725
Url: https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84948453364&partnerID=40&md5=6569aec1c5036717080cf6d0ed6aa37a
Keywords: Autoprocessing, Single-Point Mutation, X-Ray Crystallography, γ-Glutamyltranspeptidase, γ-Gt Precursor, Alanine, Bacterial Enzyme, Carbonyl Derivative, Enzyme Precursor, Gamma Glutamyltransferase, Hydroxyl Group, Threonine, Article, Bacillus Licheniformis, Controlled Study, Crystal Structure, Enzyme Activity, Enzyme Mechanism, Enzyme Structure, Hydrogen Bond, Nonhuman, Nucleophilicity, Priority Journal, Site Directed Mutagenesis, X Ray Crystallography,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone, 16, Naples, Italy
Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi City, Taiwan
References:
Castellano, I., Merlino, A., (2012) Cell. Mol. Life Sci., 69, pp. 3381-339
Stole, E., Smith, T.K., Manning, J.M., Meister, A., (1994) J. Biol. Chem., 269, pp. 21435-21439
Tate, S.S., Meister, A., (1981) Mol. Cell. Biochem., p. 39
Hanigan, M.H., Ricketts, W.A., (1993) Biochemistry, 32, pp. 6302-6306
Taniguchi, N., Ikeda, Y., (1998) Adv. Enzymol. Relat. Areas Mol. Biol., p. 72
Hanigan, M.H., Gallagher, B.C., Townsend, D.M., Gabarra, V., (1999) Carcinogenesis, 20, pp. 553-559
Wilk, S., Mizoguchi, H., Orlowski, M., (1978) J. Pharmacol. Exp. Ther., 206, pp. 227-232
Zhang, Q., Kulczynska, A., Webb, D.J., Megson, I.L., Botting, N.P., (2013) Chem. Commun., 49, pp. 1389-1391
Allison, R.D., (1985) Methods Enzymol., 113, pp. 419-437
Tate, S.S., (1986) FEBS Lett., 194, pp. 33-38
Inoue, M., Hiratake, J., Suzuki, H., Kumagai, H., Sakata, K., (2000) Biochemistry, 39, pp. 7764-7771
Xu, Q., Buckley, D., Guan, C., Guo, H.-C., (1999) Cell, 98, pp. 651-661
Qian, X., Guan, C., Guo, H.-C., (2003) Structure, 11, pp. 997-1003
Kim, Y., Yoon, K.-H., Khang, Y., Turley, S., Hol, W.G., (2000) Structure, 8, pp. 1059-1068
Hewitt, L., Kasche, V., Lummer, K., Lewis, R., Murshudov, G., Verma, C., Dodson, G., Wilson, K., (2000) J. Mol. Biol., 302, pp. 887-898
Ditzel, L., Huber, R., Mann, K., Heinemeyer, W., Wolf, D.H., Groll, M., (1998) J. Mol. Biol., 279, pp. 1187-1191
Bokhove, M., Yoshida, H., Hensgens, C.M., Van Der Laan, J.M., Sutherland, J.D., Dijkstra, B.W., (2010) Structure, 18, pp. 301-308
Minami, H., Suzuki, H., Kumagai, H., (2003) FEMS Microbiol. Lett., 224, pp. 169-173
Boanca, G., Sand, A., Barycki, J.J., (2006) J. Biol. Chem., 281, pp. 19029-19037
Hung, C.-P., Yang, J.-C., Chen, J.-H., Chi, M.-C., Lin, L.-L., (2011) J. Biol. Phys., 37, pp. 463-475
Murty, N.A., Tiwary, E., Sharma, R., Nair, N., Gupta, R., (2012) Enzym. Microb. Technol., 50, pp. 159-164
Chang, H.P., Liang, W.C., Lyu, R.C., Chi, M.C., Wang, T.F., Su, K.L., Hung, H.C., Lin, L.L., (2010) Biochemistry (Mosc), 75, pp. 919-929
Okada, T., Suzuki, H., Wada, K., Kumagai, H., Fukuyama, K., (2006) Proc. Natl. Acad. Sci. U. S. A., 103, pp. 6471-6476
Wada, K., Irie, M., Suzuki, H., Fukuyama, K., (2010) FEBS J., 277, pp. 1000-1009
Oinonen, C., Rouvinen, J., (2000) Protein Sci., p. 9
Lee, Y.S., Kim, H.W., Lee, K.B., Park, S.S., (2000) Biochim. Biophys. Acta, 1523, pp. 123-127
Kim, Y., Kim, S., Earnest, T.N., Hol, W.G., (2002) J. Biol. Chem., 277, pp. 2823-2829
West, M.B., Chen, Y., Wickham, S., Heroux, A., Cahill, K., Hanigan, M.H., Mooers, B.H., (2013) J. Biol. Chem., 288, pp. 31902-31913
Lin, L.L., Chen, Y.Y., Chi, M.C., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 1523-1529
Moallic, C., Dabonne, S., Colas, B., Sine, J.P., (2006) Protein J., 25, pp. 391-397
Castellano, I., Di Salle, A., Merlino, A., Rossi, M., Laara, F., (2011) Extremophiles, 15, pp. 259-270
Chi, M.C., Lo, Y.H., Chen, Y.Y., Lin, L.L., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 2290-2297
Lyu, R.C., Hu, H.Y., Kuo, L.Y., Lo, H.F., Ong, P.L., Chang, H.P., Lin, L.L., (2009) Curr. Microbiol., 59, pp. 101-106
Otwinowski, Z., Minor, W., (1997) Methods in Enzymology, 276, pp. 307-326. , ed. J. Charles W. Carter, Academic Press
Emsley, P., Cowtan, K., (2004) Acta Crystallogr. Sect. D: Biol. Crystallogr., 60, pp. 2126-2132
Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., Echols, N., Headd, J.J., Zwart, P.H., (2010) Acta Crystallogr. Sect. D: Biol. Crystallogr., 66, pp. 213-221
Noren, C.J., Wang, J., Perler, F.B., (2000) Angew. Chem., 39, pp. 450-466
Chi, M.C., Chen, Y.Y., Lo, H.F., Lin, L.L., (2012) FEBS Open Bio, 2, pp. 298-304
Hashimoto, W., Suzuki, H., Nohara, S., Kumagai, H., (1992) Biochem. Biophys. Res. Commun., 189, pp. 173-178
The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They are synthetized as single-chain precursors, which are then cleaved to form mature enzymes. Basic aspects of autocatalytic processing of these pro-enzymes are still unknown. Here we describe the X-ray structure of the precursor mimic of Bacillus licheniformis γ-GT (BlGT), obtained by mutating catalytically important threonine to alanine (T399A-BlGT), and report results of autoprocessing of mutants of His401, Thr415, Thr417, Glu419 and Arg571. Data suggest that Thr417 is in a competent position to activate the catalytic threonine (Thr399) for nucleophilic attack of the scissile peptide bond and that Thr415 plays a major role in assisting the process. On the basis of these new structural results, a possible mechanism of autoprocessing is proposed. This mechanism, which guesses the existence of a six-membered transition state involving one carbonyl and two hydroxyl groups, is in agreement with all the available experimental data collected on γ-GTs from different species and with our new Ala-scanning mutagenesis data. © 2015 Elsevier B.V. All rights reserved.
γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They are synthetized as single-chain precursors, which are then cleaved to form mature enzymes. Basic aspects of autocatalytic processing of these pro-enzymes are still unknown. Here we describe the X-ray structure of the precursor mimic of Bacillus licheniformis γ-GT (BlGT), obtained by mutating catalytically important threonine to alanine (T399A-BlGT), and report results of autoprocessing of mutants of His401, Thr415, Thr417, Glu419 and Arg571. Data suggest that Thr417 is in a competent position to activate the catalytic threonine (Thr399) for nucleophilic attack of the scissile peptide bond and that Thr415 plays a major role in assisting the process. On the basis of these new structural results, a possible mechanism of autoprocessing is proposed. This mechanism, which guesses the existence of a six-membered transition state involving one carbonyl and two hydroxyl groups, is in agreement with all the available experimental data collected on γ-GTs from different species and with our new Ala-scanning mutagenesis data. © 2015 Elsevier B.V. All rights reserved.
The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies
| ▼ Galectins: Structure, function and therapeutic potential inhibitors |
The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies
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Wekselman I, Davidovich C, Agmon I, Zimmerman E, Rozenberg H, Bashan A, Berisio R, Yonath A
* Ribosome's mode of function: Myths, facts and recent results (394 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 122-130.
Impact Factor: 1.807
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Alterio V, Di Fiore A, D'Ambrosio K, Supuran CT, De Simone G
* X-Ray Crystallography of Carbonic Anhydrase Inhibitors and Its Importance in Drug Design (426 views)
Drug Des Of Zinc Enzyme Inhibitors (ISSN: 9780-4702, 9780-470275009), 2009; N/D: 73-138.
Impact Factor: 1.156
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Merlino A, Sica F, Mazzarella L, Zagari A, Vergara A
* Correlation between Raman and X-ray crystallography data of (Pro-Pro-Gly)(10) (399 views)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2008 Sep; 137(1): 24-27.
Impact Factor: 2.362
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Alterio V, De Simone G, Monti SM, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: Inhibition of human, bacterial, and archaeal isozymes with benzene-1, 3-disulfonamides-Solution and crystallographic studies (329 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Sep 1; 17(15): 4201-4207.
Impact Factor: 2.604
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Di Fiore A, Scozzafava A, Winum JY, Montero JL, Pedone C, Supuran CT, De Simone G
* Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties (410 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Mar 15; 17(6): 1726-1731.
Impact Factor: 2.604
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Menchise V, De Simone G, Di Fiore A, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: X-ray crystallographic studies for the binding of 5-amino-1, 3, 4-thiadiazole-2-sulfonamide and 5-(4-amino-3-chloro-5-fluorophenylsulfonamido)1, 3, 4-thiadiazole-2-sulfon amide to human isoform II (310 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Dec 15; 16(24): 6204-6208.
Impact Factor: 2.538
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Di Fiore A, Pedone C, D'Ambrosio K, Scozzafava A, De Simone G, Supuran CT
* Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II 'selective' inhibitor celecoxib (323 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Jan 15; 16(2): 437-442.
Impact Factor: 2.538
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Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal Structure Of The Alcohol Dehydrogenase From The Hyperthermophilic Archaeon Sulfolobus Solfataricus At 1. 85 Angstrom Resolution (435 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2002 Apr 26; 318(2): 463-477.
Impact Factor: 5.359
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* Design, synthesis and biochemical evaluation of novel carbonic anhydrase inhibitors triggered by structural knowledge on hCA VII (27 views)
Bioorg Med Chem (ISSN: 0968-0896linking, 1464-3391), 2021 Aug 15; 44: 116279-116279.
Impact Factor: 3.641
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Langella E, Alterio V, D'Ambrosio K, Cadoni R, Winum JY, Supuran CT, Monti SM, De Simone G, Di Fiore A
* Exploring benzoxaborole derivatives as carbonic anhydrase inhibitors: a structural and computational analysis reveals their conformational variability as a tool to increase enzyme selectivity (243 views)
J Enzym Inhib Med Ch (ISSN: 1475-6366linking, 1475-6374electronic), 2019 Dec; 34(1): 1498-1505.
Impact Factor: 4.027
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Esposito L, Donnarumma F, Ruggiero A, Leone S, Vitagliano L, Picone D
* Structure, stability and aggregation propensity of a Ribonuclease A-Onconase chimera (292 views)
Int J Biol Macromol (ISSN: 0141-8130linking, 1879-0003electronic), 2019 Jul 15; 133: 1125-1133.
Impact Factor: 4.784
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Buemi MR, Di Fiore A, De Luca L, Angeli A, Mancuso F, Ferro S, Monti SM, Buonanno M, Russo E, De Sarro G, De Simone G, Supuran CT, Gitto R
* Exploring structural properties of potent human carbonic anhydrase inhibitors bearing a 4-(cycloalkylamino-1-carbonyl)benzenesulfonamide moiety (322 views)
Eur J Med Chem (ISSN: 1768-3254electronic, 0223-5234linking), 2019 Feb 1; 163: 443-452.
Impact Factor: 4.833
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Alterio V, Esposito D, Monti SM, Supuran CT, De Simone G
* Crystal structure of the human carbonic anhydrase II adduct with 1-(4-sulfamoylphenyl-ethyl)-2, 4, 6-triphenylpyridinium perchlorate, a membrane-impermeant, isoform selective inhibitor (533 views)
J Enzym Inhib Med Ch (ISSN: 1475-6374electronic, 1475-6366linking), 2018 Dec; 33(1): 151-157.
Impact Factor: 4.293
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Merlino A, Marzo T, Messori L
* Protein Metalation by Anticancer Metallodrugs: A Joint ESI MS and XRD Investigative Strategy (232 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2017 May 23; 23(29): 6942-6947.
Impact Factor: 5.16
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Vullo D, Supuran CT, Scozzafava A, De Simone G, Monti SM, Alterio V, Carta F
* Kinetic and X-ray crystallographic investigations of substituted 2-thio-6-oxo-1, 6-dihydropyrimidine-benzenesulfonamides acting as carbonic anhydrase inhibitors (254 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 0968-0896linking), 2016 Aug 15; 24(16): 3643-3648.
Impact Factor: 2.93
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Messori L, Marzo T, Merlino A
* Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts (312 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2015; N/D: N/D-N/D.
Impact Factor: 3.205
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D'Ambrosio K, Lopez M, Dathan NA, Ouahrani-bettache S, Köhler S, Ascione G, Monti SM, Winum JY, De Simone G
* Structural basis for the rational design of new anti-Brucella agents: The crystal structure of the C366S mutant of l-histidinol dehydrogenase from Brucella suis (452 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Feb; 97(1): 114-120.
Impact Factor: 2.963
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Chi M-C, Lo Y-H, Chen Y-Y, Lin L-L, Merlino A
* γ-Glutamyl transpeptidase architecture: Effect of extra sequence deletion on autoprocessing, structure and stability of the protein from Bacillus licheniformis (313 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014; 1844(12): 2290-2297.
Impact Factor: 4.882
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Lin L-L, Chen Y-Y, Chi M-C, Merlino A
* Low resolution X-ray structure of γ-glutamyltranspeptidase from Bacillus licheniformis: Opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion (410 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014; 1844(9): 1523-1529.
Impact Factor: 4.882
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Alterio V, Pan P, Parkkila S, Buonanno M, Supuran CT, Monti SM, De Simone G
* The Structural Comparison Between Membrane-Associated Human Carbonic Anhydrases Provides Insights Into Drug Design Of Selective Inhibitors (416 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2014; 101(7): 769-778.
Impact Factor: 2.385
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Ronda L, Merlino A, Bettati S, Verde C, Balsamo A, Mazzarella L, Mozzarelli A, Vergara A
* Role of tertiary structures on the Root effect in fish hemoglobins (481 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(9): 1885-1893.
Impact Factor: 3.191
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De Simone G, Alterio V, Supuran CT
* Exploiting the hydrophobic and hydrophilic binding sites for designing carbonic anhydrase inhibitors (507 views)
Expert Opin Drug Discov (ISSN: 1746-0441), 2013 Jul; 8(7): 793-810.
Impact Factor: 3.467
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De Simone G, Supuran CT
* (In)organic anions as carbonic anhydrase inhibitors (378 views)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2012 Jun; 111: 117-129.
Impact Factor: 3.197
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Pica A, Russo Krauss I, Castellano I, Rossi M, La Cara F, Graziano G, Sica F, Merlino A
* Exploring The Unfolding Mechanism Of Gamma-Glutamyltranspeptidases: The Case Of The Thermophilic Enzyme From Geobacillus Thermodenitrificans (396 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2012 Apr; 1824(4): 571-577.
Impact Factor: 3.733
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Russo Krauss I, Sica F, Mattia CA, Merlino A
* Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data (461 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2012 Mar; 13(3): 3782-3800.
Impact Factor: 2.464
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Esposito L, Ruggiero A, Masullo M, Ruocco MR, Lamberti A, Arcari P, Zagari A, Vitagliano L
* Crystallographic and spectroscopic characterizations of Sulfolobus solfataricus TrxA1 provide insights into the determinants of thioredoxin fold stability (300 views)
J Struct Biol (ISSN: 1047-8477, 1047-8477linking), 2012 Feb; 177(2): 506-512.
Impact Factor: 3.361
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Castellano I, Merlino A
* γ-glutamyltranspeptidases: Sequence, structure, biochemical properties, and biotechnological applications (422 views)
Cell Mol Life Sci (ISSN: 1420-682x), 2012; 69(20): 3381-3394.
Impact Factor: 5.615
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Merlino A, Russo Krauss I, Albino A, Pica A, Vergara A, Masullo M, De Vendittis E, Sica F
* Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis (361 views)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2011 Sep; 12(9): 6312-6319.
Impact Factor: 2.598
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Castellano I, Di Salle A, Merlino A, Rossi M, La Cara F
* Gene cloning and protein expression of γ-glutamyltranspeptidases from Thermus thermophilus and Deinococcus radiodurans: Comparison of molecular and structural properties with mesophilic counterparts (340 views)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2011; 15(2): 259-270.
Impact Factor: 2.941
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Di Fiore A, Monti SM, Innocenti A, Winum J-Y, De Simone G, Supuran CT
* Carbonic anhydrase inhibitors: Crystallographic and solution binding studies for the interaction of a boron-containing aromatic sulfamide with mammalian isoforms I-XV (352 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2010 Jun 15; 20(12): 3601-3605.
Impact Factor: 2.661
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De Simone G, Supuran CT
* Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target (398 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2010 Feb; 1804(2): 404-409.
Impact Factor: 2.773
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Guler OO, De Simone G, Supuran CT
* Drug design studies of the novel antitumor targets carbonic anhydrase IX and XII (540 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2010; 17(15): 1516-1526.
Impact Factor: 4.63
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Merlino A, Vitagliano L, Howes BD, Verde C, Di Prisco G, Smulevich G, Sica F, Vergara A
* Combined Crystallographic and Spectroscopic Analysis of Trematomus bernacchii Hemoglobin Highlights Analogies and Differences in the Peculiar Oxidation Pathway of Antarctic Fish Hemoglobins (413 views)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1117-1125.
Impact Factor: 2.605
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Ruggiero A, Masullo M, Marasco D, Ruocco MR, Grimaldi P, Arcari P, Zagari A, Vitagliano L
* The dimeric structure of Sulfolobus solfataricus thioredoxin A2 and the basis of its thermostability (316 views)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2009 Dec; 77(4): 1004-1008.
Impact Factor: 3.085
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Wekselman I, Davidovich C, Agmon I, Zimmerman E, Rozenberg H, Bashan A, Berisio R, Yonath A
* Ribosome's mode of function: Myths, facts and recent results (394 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 122-130.
Impact Factor: 1.807
View Export to BibTeX Export to EndNote
Alterio V, Di Fiore A, D'Ambrosio K, Supuran CT, De Simone G
* X-Ray Crystallography of Carbonic Anhydrase Inhibitors and Its Importance in Drug Design (426 views)
Drug Des Of Zinc Enzyme Inhibitors (ISSN: 9780-4702, 9780-470275009), 2009; N/D: 73-138.
Impact Factor: 1.156
View Export to BibTeX Export to EndNote
Merlino A, Sica F, Mazzarella L, Zagari A, Vergara A
* Correlation between Raman and X-ray crystallography data of (Pro-Pro-Gly)(10) (399 views)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2008 Sep; 137(1): 24-27.
Impact Factor: 2.362
View Export to BibTeX Export to EndNote
Alterio V, De Simone G, Monti SM, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: Inhibition of human, bacterial, and archaeal isozymes with benzene-1, 3-disulfonamides-Solution and crystallographic studies (329 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Sep 1; 17(15): 4201-4207.
Impact Factor: 2.604
View Export to BibTeX Export to EndNote
Di Fiore A, Scozzafava A, Winum JY, Montero JL, Pedone C, Supuran CT, De Simone G
* Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties (410 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Mar 15; 17(6): 1726-1731.
Impact Factor: 2.604
View Export to BibTeX Export to EndNote
Menchise V, De Simone G, Di Fiore A, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: X-ray crystallographic studies for the binding of 5-amino-1, 3, 4-thiadiazole-2-sulfonamide and 5-(4-amino-3-chloro-5-fluorophenylsulfonamido)1, 3, 4-thiadiazole-2-sulfon amide to human isoform II (310 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Dec 15; 16(24): 6204-6208.
Impact Factor: 2.538
View Export to BibTeX Export to EndNote
Di Fiore A, Pedone C, D'Ambrosio K, Scozzafava A, De Simone G, Supuran CT
* Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II 'selective' inhibitor celecoxib (323 views)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Jan 15; 16(2): 437-442.
Impact Factor: 2.538
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal Structure Of The Alcohol Dehydrogenase From The Hyperthermophilic Archaeon Sulfolobus Solfataricus At 1. 85 Angstrom Resolution (435 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2002 Apr 26; 318(2): 463-477.
Impact Factor: 5.359
View Export to BibTeX Export to EndNote
34 Records (34 excluding Abstracts).
Total impact factor: 115.274 (115.274 excluding Abstracts).
Total 5 year impact factor: 107.266 (107.266 excluding Abstracts).
Total impact factor: 115.274 (115.274 excluding Abstracts).
Total 5 year impact factor: 107.266 (107.266 excluding Abstracts).
342 views. Last view on Tuesday 07 March 2023, 12:11:13
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