Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces(477 views) La Manna S, Scognamiglio PL, Di Natale C, Leone M, Mercurio FA, Malfitano AM, Cianfarani F, Madonna S, Caravella S, Albanesi C, Novellino E, Marasco D
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2017 May 04; 138: 106-115.
Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples "Federico II", 80134, Naples, Italy., Institute of Biostructures and Bioimaging, CNR, 80134, Naples, Italy., Laboratory of Cellular and Molecular Biology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy., Laboratory of Experimental Immunology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy., Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples "Federico II", 80134, Naples, Italy. Electronic address: daniela.marasco@unina.it.,
Institute of Biostructures and Bioimaging, CNR, 80134, Naples, Italy.
Laboratory of Cellular and Molecular Biology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy.
Laboratory of Experimental Immunology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy.
References: Not available.
Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces
Interleukin-22 (IL-22) belongs to the family of IL-10 cytokines and is involved in a wide number of human diseases, including inflammatory disorders and cancer pathology. The ligand-receptor complex IL-22/IL-22R plays a key role in several pathways especially in the regulation and resolution of immune responses. The identification of novel compounds able to modulate IL-22/IL-22R complex could open the route to new therapeutic strategies in multiple human diseases. In this study, we designed and characterized IL-22 derived peptides at protein interface regions: several sequences revealed able to interfere with the protein complex with IC(50) in the micromolar range as evaluated through Surface Plasmon Resonance (SPR) experiments. Their conformational characterization was carried out through Circular Dichroism (CD) and Nuclear Magnetic Resonance (NMR) spectroscopies, shedding new light into the features of IL-22 fragments and on structural determinants of IL-22/IL-22R1 recognition. Finally, several peptides were tested on human keratinocyte cultures for evaluating their ability to mimic the activation of molecular pathways downstream to IL-22R in response to IL-22 binding.
Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces
Kállay C, Dávid A, Timári S, Nagy EM, Sanna D, Garribba E, Micera G, De Bona P, Pappalardo G, Rizzarelli E, Sóvágó I * Copper(II) complexes of rat amylin fragments(480 views) Dalton T (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2011 Oct 14; 40(38): 9711-9721. Impact Factor:3.838 ViewExport to BibTeXExport to EndNote
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(532 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote