Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase
Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase(268 views)(PDF public186 views) Aulitto M, Fusco S, Fiorentino G, Limauro D, Pedone E, Bartolucci S, Contursi P
Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase
BACKGROUND:
The
genus Thermus, which has been considered for a long time as a fruitful
source of biotechnological relevant enzymes, has emerged more recently
as suitable host to overproduce thermozymes. Among these,
α-galactosidases are widely used in several industrial bioprocesses that
require high working temperatures and for which thermostable variants
offer considerable advantages over their thermolabile counterparts.
RESULTS:
Thermus
thermophilus HB27 strain was used for the homologous expression of the
TTP0072 gene encoding for an α-galactosidase (TtGalA). Interestingly, a
soluble and active histidine-tagged enzyme was produced in larger
amounts (5 mg/L) in this thermophilic host than in Escherichia coli
(0.5 mg/L). The purified recombinant enzyme showed an optimal activity
at 90 °C and retained more than 40% of activity over a broad range of pH
(from 5 to 8).
CONCLUSIONS:
TtGalA
is among the most thermoactive and thermostable α-galactosidases
discovered so far, thus pointing to T. thermophilus as cell factory for
the recombinant production of biocatalysts active at temperature values
over 90 °C.
Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase
Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase
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