A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction (1484 views) (PDF public 178 views)
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220linking), 2017 Nov 28; 17(12): N/D-N/D.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.475, 5-year impact factor: 3.014
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037068916&doi=10.3390%2fs17122747&partnerID=40&md5=38d8490185afe49e9272525e3f00707f
Keywords: Qcm Immunosensor, Anti-Cd34 Antibody, Dental Pulp, Self-Assembled Monolayer, Stem Cells, Antibodies, Cell Culture, Cell Immobilization, Cell Proliferation, Cost Effectiveness, Cytology, Gold Coatings, Quartz, Self Assembled Monolayers, Antibody Immobilization, Cell Populations, Real-Time Detection, Recognition Mechanism, Saving Operations, Simultaneous Detection, Quartz Crystal Microbalances, Silicon Dioxide, Genetic Procedures, Immunoassay, Biosensing Techniques, Quartz Crystal Microbalance Techniques,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. ornella.maglio@unina.it., Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy. ornella.maglio@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. salvatore.costanzo@upmc.fr., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. rc1274@york.ac.uk., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. gerardo.zambrano@unina.it., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. mauro@novaetech.com., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. battaglia@novaetech.com., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. ferrini@novaetech.com., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. flavia.nastri@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. vincenzo.pavone@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. alombard@unina.it.,
Faculty of Chemistry, University Pierre et Marie Curie, 4 Place Jussieu, Paris, 75005, France
References:
Not available.
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220linking), 2017 Nov 28; 17(12): N/D-N/D.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.475, 5-year impact factor: 3.014
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037068916&doi=10.3390%2fs17122747&partnerID=40&md5=38d8490185afe49e9272525e3f00707f
Keywords: Qcm Immunosensor, Anti-Cd34 Antibody, Dental Pulp, Self-Assembled Monolayer, Stem Cells, Antibodies, Cell Culture, Cell Immobilization, Cell Proliferation, Cost Effectiveness, Cytology, Gold Coatings, Quartz, Self Assembled Monolayers, Antibody Immobilization, Cell Populations, Real-Time Detection, Recognition Mechanism, Saving Operations, Simultaneous Detection, Quartz Crystal Microbalances, Silicon Dioxide, Genetic Procedures, Immunoassay, Biosensing Techniques, Quartz Crystal Microbalance Techniques,
Affiliations:
*** IBB - CNR ***
Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. ornella.maglio@unina.it., Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy. ornella.maglio@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. salvatore.costanzo@upmc.fr., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. rc1274@york.ac.uk., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. gerardo.zambrano@unina.it., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. mauro@novaetech.com., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. battaglia@novaetech.com., Novaetech S.r.l., Centro Direzionale, Isola G7, 80143 Napoli, Italy. ferrini@novaetech.com., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. flavia.nastri@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. vincenzo.pavone@unina.it., Department of Chemical Sciences, University of Napoli "Federico II" Via Cintia, 80126 Napoli, Italy. alombard@unina.it.,
Faculty of Chemistry, University Pierre et Marie Curie, 4 Place Jussieu, Paris, 75005, France
References:
Not available.
A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction
A cost-effective immunosensor for the detection and isolation of dental pulp stemcells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specificmonoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCMsurface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with amixed cell population, was analyzed; the results indicated that the developed QCMimmunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell fromsampleswith high complexity, being simple, selective, and providing for more convenient and time-saving operations. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
A cost-effective immunosensor for the detection and isolation of dental pulp stemcells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specificmonoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCMsurface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with amixed cell population, was analyzed; the results indicated that the developed QCMimmunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell fromsampleswith high complexity, being simple, selective, and providing for more convenient and time-saving operations. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction
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A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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Impact Factor: 5.546
View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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Impact Factor: 4.556
View Export to BibTeX Export to EndNote
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Impact Factor: 4.041
View Export to BibTeX Export to EndNote
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Impact Factor: 7.36
View Export to BibTeX Export to EndNote
Fagoonee S, Famulari ES, Silengo L, Tolosano E, Altruda F
* Long Term Liver Engraftment of Functional Hepatocytes Obtained from Germline Cell-Derived Pluripotent Stem Cells (298 views)
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Impact Factor: 3.057
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De Chiara L, Fagoonee S, Ranghino A, Bruno S, Camussi G, Tolosano E, Silengo L, Altruda F
* Renal cells from spermatogonial germline stem cells protect against kidney injury (502 views)
J Am Soc Nephrol (ISSN: 1046-6673), 2014 Feb; 25(2): 316-328.
Impact Factor: 9.343
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Severino V, Alessio N, Farina A, Sandomenico A, Cipollaro M, Peluso G, Galderisi U, Chambery A
* Insulin-like growth factor binding proteins 4 and 7 released by senescent cells promote premature senescence in mesenchymal stem cells (3878 views)
Cell Death & (ISSN: 2041-4889, 2041-4889electronic), 2013 Nov 7; 4(11): e911-e911.
Impact Factor: 5.177
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Romano S, Staibano S, Greco A, Brunetti A, Nappo G, Ilardi G, Martinelli R, Sorrentino A, Di Pace A, Mascolo M, Bisogni R, Scalvenzi M, Alfano B, Romano M
* FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential (659 views) (PDF 271 views)
Cell Death & (ISSN: 2041-4889, 2041-4889electronic), 2013 Apr 4; 4(6): e578-e578.
Impact Factor: 5.333
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Galasso G, De Rosa R, Ciccarelli M, Sorriento D, Del Giudice C, Strisciuglio T, De Biase C, Luciano R, Piccolo R, Pierri A, Di Gioia G, Prevete N, Trimarco B, Piscione F, Iaccarino G
* β2-adrenergic receptor stimulation improves endothelial progenitor cell-mediated ischemic neoangiogenesis (585 views)
Circ Res (ISSN: 0009-7330, 1524-4571electronic, 0009-7330linking), 2013; 112(7): 1026-1034.
Impact Factor: 11.089
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Riccio A, Cubellis MV
* Gain of function in CDKN1C (591 views)
Nature Genetics (ISSN: 1061-4036), 2012 Jul; 44(7): 737-738.
Impact Factor: 35.209
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Lonardo E, Parish CL, Ponticelli S, Marasco D, Ribeiro D, Ruvo M, De Falco S, Arenas E, Minchiotti G
* A Small Synthetic Cripto Blocking Peptide Improves Neural Induction, Dopaminergic Differentiation, and Functional Integration of Mouse Embryonic Stem Cells in a Rat Model of Parkinson's Disease (903 views)
Stem Cells (ISSN: 1066-5099), 2010 Sep; 28(8): 1326-1337.
Impact Factor: 7.871
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Kelly SE, Di Benedetto A, Greco A, Howard CM, Sollars VE, Primerano DA, Valluri JV, Claudio PP
* Rapid Selection and Proliferation of CD133(+) Cells from Cancer Cell Lines: Chemotherapeutic Implications (653 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2010 Apr 8; 5(4): N/D-N/D.
Impact Factor: 4.411
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Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Del Vecchio L, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M
* MicroRNA-199b-5p Impairs Cancer Stem Cells through Negative Regulation of HES1 in Medulloblastoma (552 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2009 Mar 24; 4(3): e4998-e4998.
Impact Factor: 4.351
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Chambery A, Vissers JPC, Langridge JI, Lonardo E, Minchiotti G, Ruvo M, Parente A
* Qualitative and quantitative proteomic profiling of cripto-1- embryonic stem cells by means of accurate mass LC-MS analysis (1294 views)
J Proteome Res (ISSN: 1535-3893), 2009; 8(2): 1047-1058.
Impact Factor: 5.132
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Castiglione M, Calafiore M, Costa L, Sortino MA, Nicoletti F, Copani A
* Group I metabotropic glutamate receptors control proliferation, survival and differentiation of cultured neural progenitor cells isolated from the subventricular zone of adult mice (764 views)
Neuropharm (ISSN: 0028-3908, 1873-7064electronic, 0028-3908linking), 2008 Sep; 55(4): 560-567.
Impact Factor: 3.383
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29 Records (23 excluding Abstracts).
Total impact factor: 189.788 (158.116 excluding Abstracts).
Total 5 year impact factor: 146.373 (113.531 excluding Abstracts).
Total impact factor: 189.788 (158.116 excluding Abstracts).
Total 5 year impact factor: 146.373 (113.531 excluding Abstracts).
1484 views. Last view on Tuesday 10 December 2024, 8:47:14
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