Faculty of Chemistry, Department of Organic Chemistry, University of Lodz, Tamka 12, 91-403 Lodz, Poland. asiaskiba@02.pl., Nencki Institute of Experimental Biology, Polish Academy of Sciences, ul. Pasteura 3, 02-093 Warszawa, Poland. t.bernas@nencki.gov.pl., Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-089 Warszawa, Poland. trzybinski@chem.uw.edu.pl., Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-089 Warszawa, Poland. kwozniak@chem.uw.edu.pl., Department of Chemical Sciences, University of Naples Federico II, Complesso Univ. di Monte Sant' Angelo, Via Cintia, I-80126 Napoli, Italy. giarita.ferraro@unina.it., Department of Pharmacy, University of Naples Federico II, Via Mezzocannone 16, 80134 Napoli, Italy. daniela.marasco@unina.it., CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, Via Mezzocannone 16, I-80134 Napoli, Italy. daniela.marasco@unina.it., CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, I-80134 Napoli, Italy. daniela.marasco@unina.it., Department of Chemical Sciences, University of Naples Federico II, Complesso Univ. di Monte Sant' Angelo, Via Cintia, I-80126 Napoli, Italy. antonello.merlino@unina.it., CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, I-80134 Napoli, Italy. antonello.merlino@unina.it., Department of Technology of Organic Synthesis, Institute of Chemical Engineering, Ural Federal University, 19 Mira Str., 620002 Ekaterinburg, Russia. shafikoff@gmail.com., Institut fur Physikalische und Theoretische Chemie, Universitat Regensburg, Universitatsstrasse 31, D-93040 Regensburg, Germany. shafikoff@gmail.com., Institut fur Physikalische und Theoretische Chemie, Universitat Regensburg, Universitatsstrasse 31, D-93040 Regensburg, Germany. Rafal.Czerwieniec@uni-bayreuth.de., Lehrstuhl fur Anorganische Chemie I, University of Bayreuth, D-95440 Bayreuth, Germany. Rafal.Czerwieniec@uni-bayreuth.de., Faculty of Chemistr
References: Not available.
Mitochondria Targeting with Luminescent Rhenium(I) Complexes
Two new neutral fac-[Re(CO)(3)(phen)L] compounds (1,2), with phen = 1,10-phenanthroline and L = O(2)C(CH(2))(5)CH(3) or O(2)C(CH(2))(4)C identical withCH, were synthetized in one-pot procedures from fac-[Re(CO)(3)(phen)Cl] and the corresponding carboxylic acids, and were fully characterized by IR and UV-Vis absorption spectroscopy, (1)H- and (13)C-NMR, mass spectrometry and X-ray crystallography. The compounds, which display orange luminescence, were used as probes for living cancer HeLa cell staining. Confocal microscopy revealed accumulation of both dyes in mitochondria. To investigate the mechanism of mitochondrial staining, a new non-emissive compound, fac-[Re(CO)(3)(phen)L], with L = O(2)C(CH(2))(3)((C(5)H(5))Fe(C(5)H(4)), i.e., containing a ferrocenyl moiety, was synthetized and characterized (3). 3 shows the same mitochondrial accumulation pattern as 1 and 2. Emission of 3 can only be possible when ferrocene-containing ligand dissociates from the metal center to produce a species containing the luminescent fac-[Re(CO)(3)(phen)](+) core. The release of ligands from the Re center was verified in vitro through the conjugation with model proteins. These findings suggest that the mitochondria accumulation of compounds 1-3 is due to the formation of luminescent fac-[Re(CO)(3)(phen)](+) products, which react with cellular matrix molecules giving secondary products and are uptaken into the negatively charged mitochondrial membranes. Thus, reported compounds feature a rare dissociation-driven mechanism of action with great potential for biological applications.
Mitochondria Targeting with Luminescent Rhenium(I) Complexes
No results.
Mitochondria Targeting with Luminescent Rhenium(I) Complexes