Mechanism of translation control of the alternative Drosophila melanogaster Voltage Dependent Anion-selective Channel 1 mRNAs(483 views) Leggio L, Guarino F, Magrì A, Accardi-gheit R, Reina S, Specchia V, Damiano F, Tomasello MF, Tommasino M, Messina A
Department of Biological, University of Catania, Geological and Environmental Sciences, Catania, 95125, Italy
Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, 95123, Italy
National Institute of Biostructures and Biosystems (INBB), Catania, Italy
International Agency for Research on Cancer (IARC), World Health Organization, Lyon, 69372, France
IBB-CNR, Institute of Biostructure and Bioimaging, Section of Catania, Via Paolo Gaifami, 18, Catania, 95126, Italy
References: Not available.
Mechanism of translation control of the alternative Drosophila melanogaster Voltage Dependent Anion-selective Channel 1 mRNAs
The eukaryotic porin, also called the Voltage Dependent Anion-selective
Channel (VDAC), is the main pore-forming protein of the outer
mitochondrial membrane. In Drosophila melanogaster, a cluster of genes
evolutionarily linked to VDAC is present on chromosome 2L. The main VDAC
isoform, called VDAC1 (Porin1), is expressed from the first gene of the
cluster. The porin1 gene produces two splice variants, 1A-VDAC and
1B-VDAC, with the same coding sequence but different 5' untranslated
regions (UTRs). Here, we studied the influence of the two 5' UTRs, 1A-5'
UTR and 1B-5' UTR, on transcription and translation of VDAC1 mRNAs. In
porin-less yeast cells, transformation with a construct carrying 1A-VDAC
results in the expression of the corresponding protein and in
complementation of a defective cell phenotype, whereas the 1B-VDAC
sequence actively represses VDAC expression. Identical results were
obtained using constructs containing the two 5' UTRs upstream of the GFP
reporter. A short region of 15 nucleotides in the 1B-5' UTR should be
able to pair with an exposed helix of 18S ribosomal RNA (rRNA), and this
interaction could be involved in the translational repression. Our data
suggest that contacts between the 5' UTR and 18S rRNA sequences could
modulate the translation of Drosophila 1B-VDAC mRNA. The evolutionary
significance of this finding is discussed.
Mechanism of translation control of the alternative Drosophila melanogaster Voltage Dependent Anion-selective Channel 1 mRNAs
No results.
Mechanism of translation control of the alternative Drosophila melanogaster Voltage Dependent Anion-selective Channel 1 mRNAs