Shedding light on the interaction of polydatin and resveratrol with G-quadruplex and duplex DNA: a biophysical, computational and biological approach(309 views) Platella C, Raucci U, Rega N, D’atri S, Levati L, Roviello GN, Fuggetta MP, Musumeci D, Montesarchio D
Int J Biol Macromol (ISSN: 0141-8130linking, 1879-0003electronic), 2020 May 15; 151: 1163-1172.
Keywords: G-Quadruplex, Melanoma Cells, Trans-Polydatin And Trans-Resveratrol,
Affiliations: *** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia 21, Naples I-80126, Italy.
Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Piazzale Tecchio 80, Naples I-80125, Italy.
Laboratory of Molecular Oncology, IDI-IRCCS, Via dei Monti di Creta 104, Rome I-00167, Italy.
Institute of Biostructures and Bioimages, CNR, Via Mezzocannone 16, Naples I-80134, Italy.
Institute of Translational Pharmacology, CNR, Via Fosso del Cavaliere 100, Rome I-00133, Italy. Electronic address: mariapia.fuggetta@ift.cnr.it.
References: Not available.
Shedding light on the interaction of polydatin and resveratrol with G-quadruplex and duplex DNA: a biophysical, computational and biological approach
Among polyphenols, trans-resveratrol (tRES) and trans-polydatin (tPD) exert multiple biological effects, particularly antioxidant and antiproliferative. In this work, we have investigated the interaction of tPD with three cancer-related DNA sequences able to form G-quadruplex (G4) structures, as well as with a model duplex, and compared its behaviour with tRES. Interestingly, fluorescence analysis evidenced the ability of tPD to bind all the studied DNA systems, similarly to tRES, with tRES displaying a higher ability to discriminate G4 over duplex with respect to tPD. However, neither tRES nor tPD produced significant conformational changes of the analyzed DNA upon binding, as determined by CD-titration analysis. Computational analysis and biological data confirmed the biophysical results: indeed, molecular docking evidenced the stronger interaction of tRES with the promoter of c-myc oncogene, and immunoblotting assays revealed a reduction of c-myc expression, more effective for tRES than tPD. Furthermore, in vitro assays on melanoma cells proved that tPD was able to significantly reduce telomerase activity, and inhibit cell proliferation, with tRES producing higher effects than tPD.
Shedding light on the interaction of polydatin and resveratrol with G-quadruplex and duplex DNA: a biophysical, computational and biological approach