Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids (90 views)
Front Mol Neurosci (ISSN: 1662-5099linking), 2019 Aug 16; 12: 195-195.
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Paper type: Journal Article
Impact factor: 0, 5-year impact factor: 0
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Keywords: Cstb , Epm1a , Cerebral Organoids , Local Protein Synthesis , Synaptic Plasticity , Synaptosomes
Affiliations:
*** IBB - CNR ***
Department of Biology, University of Naples Federico II, Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, Italy.
Division of Molecular Biology, Biomedical Center, Faculty of Medicine, LMU, Munich, Germany.
Institute of Biostructures and Bioimaging, National Research Council (CNR), Naples, Italy.
Department of Pharmacy, University of Naples Federico II, Naples, Italy.
Institute of Genetics and Biophysics "Adriano Buzzati Traverso", National Research Council (CNR), Naples, Italy.
Department of Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
References:
Not available.
Front Mol Neurosci (ISSN: 1662-5099linking), 2019 Aug 16; 12: 195-195.
Export to BibTeX Export to EndNote
Paper type: Journal Article
Impact factor: 0, 5-year impact factor: 0
Url: Not available.
Keywords: Cstb , Epm1a , Cerebral Organoids , Local Protein Synthesis , Synaptic Plasticity , Synaptosomes
Affiliations:
*** IBB - CNR ***
Department of Biology, University of Naples Federico II, Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, Italy.
Division of Molecular Biology, Biomedical Center, Faculty of Medicine, LMU, Munich, Germany.
Institute of Biostructures and Bioimaging, National Research Council (CNR), Naples, Italy.
Department of Pharmacy, University of Naples Federico II, Naples, Italy.
Institute of Genetics and Biophysics "Adriano Buzzati Traverso", National Research Council (CNR), Naples, Italy.
Department of Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany.
References:
Not available.
Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids
Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases.
Cystatin B (CSTB) is a ubiquitous protein belonging to a superfamily of protease inhibitors. CSTB may play a critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form of progressive myoclonic epilepsy. However, the molecular mechanisms underlying the role of CSTB in the central nervous system (CNS) are largely unknown. To investigate the possible involvement of CSTB in the synaptic plasticity, we analyzed its expression in synaptosomes as a model system in studying the physiology of the synaptic regions of the CNS. We found that CSTB is not only present in the synaptosomes isolated from rat and mouse brain cortex, but also secreted into the medium in a depolarization-controlled manner. In addition, using biorthogonal noncanonical amino acid tagging (BONCAT) procedure, we demonstrated, for the first time, that CSTB is locally synthesized in the synaptosomes. The synaptic localization of CSTB was confirmed in a human 3D model of cortical development, namely cerebral organoids. Altogether, these results suggest that CSTB may play a role in the brain plasticity and open a new perspective in studying the involvement of CSTB deregulation in neurodegenerative and neuropsychiatric diseases.
Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids
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Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids
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Impact Factor: 3.383
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Travaglia A, La Mendola D, Magrì A, Nicoletti VG, Pietropaolo A, Rizzarelli E
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Impact Factor: 5.831
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* Functional partnership between mGlu3 and mGlu5 metabotropic glutamate receptors in the central nervous system (468 views)
Neuropharm (ISSN: 0028-3908linking, 1873-7064electronic), 2018 Jan; 128: 301-313.
Impact Factor: 3.383
View Export to BibTeX Export to EndNote
Travaglia A, La Mendola D, Magrì A, Nicoletti VG, Pietropaolo A, Rizzarelli E
* Copper, BDNF and Its N-terminal domain: inorganic features and biological perspectives (625 views)
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2012 Dec 3; 18(49): 15618-15631.
Impact Factor: 5.831
View Export to BibTeX Export to EndNote
2 Records (2 excluding Abstracts).
Total impact factor: 9.214 (9.214 excluding Abstracts).
Total 5 year impact factor: 9.36 (9.36 excluding Abstracts).
Total impact factor: 9.214 (9.214 excluding Abstracts).
Total 5 year impact factor: 9.36 (9.36 excluding Abstracts).
90 views. Last view on Saturday 23 March 2024, 21:50:32
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