Detection of small DNA fragments by biolayer interferometry (151 views)
Anal Biochem (ISSN: 0003-2697linking, 1096-0309electronic), 2020 Oct 15; 607: 113898-113898.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.219, 5-year impact factor: 3.039
Url: Not available.
Keywords: Biolayer Interferometry, Biosensors, Molecular Quantification, Small Dna Hybridization Sensor,
Affiliations:
*** IBB - CNR ***
Cerict Scarl, Italy. Electronic address: angelamaria.cusano@cerict.it.
Dipartimento di Ingegneria, Università Del Sannio, Italy.
Istituto di Biostrutture e Bioimmagini, CNR, Italy. Electronic address: menotti.ruvo@unina.it.
References:
Not available.
Anal Biochem (ISSN: 0003-2697linking, 1096-0309electronic), 2020 Oct 15; 607: 113898-113898.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 2.219, 5-year impact factor: 3.039
Url: Not available.
Keywords: Biolayer Interferometry, Biosensors, Molecular Quantification, Small Dna Hybridization Sensor,
Affiliations:
*** IBB - CNR ***
Cerict Scarl, Italy. Electronic address: angelamaria.cusano@cerict.it.
Dipartimento di Ingegneria, Università Del Sannio, Italy.
Istituto di Biostrutture e Bioimmagini, CNR, Italy. Electronic address: menotti.ruvo@unina.it.
References:
Not available.
Detection of small DNA fragments by biolayer interferometry
Small molecular weight species such as miRNAs and other nucleic acid fragments are gaining an increased interest as biomarkers for relevant diseases. Also, cheap and rapid assays for their routine detection are becoming an urgent need. We have investigated the usability and convenience of a price affordable, label free and fast technique for their detection on a laboratory scale small device based on Bio-Layer Interferometry. Using a model DNA fragment (7 kDa), we have found that the technique is effectively fast and sensitive enough for the detection of nucleic acid fragments having a MW below the stated molecular size detection limit (10 kDa). The test molecule has been detected in solution at 100 nM in a direct capture experiment and up to about 10 nM following an improved approach where an enhancing probe is used to increase the apparent molecular dimensions of the analyte. The technique, following further optimizations, can be applied for the routine, cheap and fast analysis of small nucleic acid fragments that have a relevance in diagnosis and in therapy.
Small molecular weight species such as miRNAs and other nucleic acid fragments are gaining an increased interest as biomarkers for relevant diseases. Also, cheap and rapid assays for their routine detection are becoming an urgent need. We have investigated the usability and convenience of a price affordable, label free and fast technique for their detection on a laboratory scale small device based on Bio-Layer Interferometry. Using a model DNA fragment (7 kDa), we have found that the technique is effectively fast and sensitive enough for the detection of nucleic acid fragments having a MW below the stated molecular size detection limit (10 kDa). The test molecule has been detected in solution at 100 nM in a direct capture experiment and up to about 10 nM following an improved approach where an enhancing probe is used to increase the apparent molecular dimensions of the analyte. The technique, following further optimizations, can be applied for the routine, cheap and fast analysis of small nucleic acid fragments that have a relevance in diagnosis and in therapy.
Detection of small DNA fragments by biolayer interferometry
Detection of small DNA fragments by biolayer interferometry
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View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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Impact Factor: 0.506
View Export to BibTeX Export to EndNote
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Lect Notes Electr Eng, 2010 May 15; 91LNEE: 485-488.
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View Export to BibTeX Export to EndNote
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Impact Factor: 5.361
View Export to BibTeX Export to EndNote
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Impact Factor: 3.322
View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
17 Records (13 excluding Abstracts).
Total impact factor: 51.516 (50.148 excluding Abstracts).
Total 5 year impact factor: 46.799 (45.487 excluding Abstracts).
Total impact factor: 51.516 (50.148 excluding Abstracts).
Total 5 year impact factor: 46.799 (45.487 excluding Abstracts).
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