Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring(261 views) Susana F, Fagoonee S, Alessandro S, Valentina G, Chiara R, Marco P, Fiorella A, Danilo D
Talanta (ISSN: 0039-9140linking), 2021 Jan 15; 222: 121441-121441.
Keywords: Cell Monitoring, Chemoresistance, Colorectal Cancer, Impedance, Biosensing Techniques
, Cell Culture Techniques
, Colonic Neoplasms Drug Therapy
, Drug Resistance
, Electric Impedance
, Humans
, Pharmaceutical Preparations,
Affiliations: *** IBB - CNR ***
Department of Electronics and Telecommunications (DET), Politecnico di Torino, Turin, Italy. Electronic address: susana.fuentes@polito.it.
Institute of Biostructure and Bioimaging (CNR), Molecular Biotechnology Center (MBC), Turin, Italy.
Eltek S.p.A, Casale Monferrato, Alessandria, Italy.
Department of Oncology and Interdepartmental Center of Research in Molecular Biotechnology, University of Turin, Turin, Italy.
Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center (MBC), University of Turin, Turin, Italy.
References: Not available.
Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring
Interest in impedance-based cellular assays is rising due to their remarkable advantages, including label-free, low cost, non-invasive, non-destructive, quantitative and real-time monitoring. In order to test their potential in cancer treatment decision and early detection of chemoresistance, we devised a new custom-made impedance measuring system based on electric cell-substrate impedance sensing (ECIS), optimized for long term impedance measurements. This device was employed in a proof of concept cell culture impedance analysis for the characterization of chemo-resistant colon cancer cells. Doxorubicin-resistant HT-29 cells were used for this purpose and monitored for 140 h. Analysis of impedance-based curves reveal different trends from chemo-sensitive and chemo-resistant cells. An impedance-based cytoxicity assay with different concentrations of doxorubicin was also performed using ECIS. The obtained results confirm the feasibility of ECIS in the study of drug resistance and show promises for studies of time-dependent factors related to physiological and behavioral changes in cells during resistance acquisition. The methodology presented herein, allows the continuous monitoring of cells under normal culture conditions as well as upon drug exposure. The ECIS device used, sets the basis for high-throughput early detection of resistance to drugs, administered in the clinical practice to cancer patients, and for the screening of new drugs in vitro, on patient-derived cells.
Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring
No results.
Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring