Amyloid-like Prep1 peptides exhibit reversible blue-green-red fluorescence in vitro and in living cells(100 views) Monti A, Bruckmann C, Blasi F, Ruvo M, Vitagliano L, Doti N
Keywords: A549 Cells, Fluorescence, Homeodomain Proteins Chemistry, Humans, Hydrogen-Ion Concentration, Optical Imaging, Protein Aggregates, Protein Conformation,
Affiliations: *** IBB - CNR ***
Institute of Biostructures and Bioimaging (IBB)-CNR, Via Mezzocannone 16, Naples 80134, Italy. luigi.vitagliano@cnr.it nunzianna.doti@cnr.it.
References: Not available.
Amyloid-like Prep1 peptides exhibit reversible blue-green-red fluorescence in vitro and in living cells
PREP1-based peptides form amyloid-like aggregates endowed with an intrinsic blue-green-red fluorescence with an unusual sharp maximum at 520 nm upon excitation with visible light under physiological conditions. The peptide PREP1[117-132], whose sequence does not contain aromatic residues, presents a pH-dependent and reversible fluorescence, in line with its structural transition from β-sheet rich aggregates to α-helix structures. These findings further demonstrate that the non-canonical fluorescence exhibited by amyloids is an articulated phenomenon.
Amyloid-like Prep1 peptides exhibit reversible blue-green-red fluorescence in vitro and in living cells