Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells (63 views)
Front Genet (ISSN: 1664-8021electronic), 2022 Jan 12; 12: N/D-N/D.
Export to BibTeX Export to EndNote
Paper type: Journal Article
Impact factor: 0, 5-year impact factor: 0
Url: Not available.
Keywords: Not available.
Affiliations:
*** IBB - CNR ***
School of Life Sciences, Manipal Academy of Higher Education, Dubai, United Arab Emirates
Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Saudi Arabia
Molecular Biotechnology Center, Institute of Biostructure and Bioimaging (CNR), Turin, Italy
Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, Saudi Arabia
References:
Not available.
Front Genet (ISSN: 1664-8021electronic), 2022 Jan 12; 12: N/D-N/D.
Export to BibTeX Export to EndNote
Paper type: Journal Article
Impact factor: 0, 5-year impact factor: 0
Url: Not available.
Keywords: Not available.
Affiliations:
*** IBB - CNR ***
School of Life Sciences, Manipal Academy of Higher Education, Dubai, United Arab Emirates
Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Saudi Arabia
Molecular Biotechnology Center, Institute of Biostructure and Bioimaging (CNR), Turin, Italy
Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, Saudi Arabia
References:
Not available.
Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells
Purpose: Plant-derived phytochemicals have shown epigenetic modulatory effect in different types of cancer by reversing the pattern of DNA methylation and chromatin modulation, thereby restoring the function of silenced tumor-suppressor genes. In the present study, attempts have been made to explore chrysin-mediated epigenetic alterations in HeLa cells.Methods: Colony formation and migration assays followed by methylation-specific PCR for examining the methylation status of CpG promoters of various tumor-suppressor genes (TSGs) and the expression of these TSGs at the transcript and protein levels were performed. Furthermore, global DNA methylation; biochemical activities of DNA methyltransferases (DNMTs), histone methyl transferases (HMTs), histone deacetylases (HDACs), and histone acetyl transferases (HATs) along with the expression analysis of chromatin-modifying enzymes; and H3 and H4 histone modification marks analyses were performed after chrysin treatment.Results: The experimental analyses revealed that chrysin treatment encourages cytostatic behavior as well as inhibits the migration capacity of HeLa cells in a time- and dose-dependent manner. Chrysin reduces the methylation of various tumor-suppressor genes, leading to their reactivation at mRNA and protein levels. The expression levels of various chromatin-modifying enzymes viz DNMTs, HMTs, HDACs, and HATS were found to be decreased, and H3 and H4 histone modification marks were modulated too. Also, reduced global DNA methylation was observed following the treatment of chrysin.Conclusion: This study concludes that chrysin can be used as a potential epigenetic modifier for cancer treatment and warrants for further experimental validation.
Purpose: Plant-derived phytochemicals have shown epigenetic modulatory effect in different types of cancer by reversing the pattern of DNA methylation and chromatin modulation, thereby restoring the function of silenced tumor-suppressor genes. In the present study, attempts have been made to explore chrysin-mediated epigenetic alterations in HeLa cells.Methods: Colony formation and migration assays followed by methylation-specific PCR for examining the methylation status of CpG promoters of various tumor-suppressor genes (TSGs) and the expression of these TSGs at the transcript and protein levels were performed. Furthermore, global DNA methylation; biochemical activities of DNA methyltransferases (DNMTs), histone methyl transferases (HMTs), histone deacetylases (HDACs), and histone acetyl transferases (HATs) along with the expression analysis of chromatin-modifying enzymes; and H3 and H4 histone modification marks analyses were performed after chrysin treatment.Results: The experimental analyses revealed that chrysin treatment encourages cytostatic behavior as well as inhibits the migration capacity of HeLa cells in a time- and dose-dependent manner. Chrysin reduces the methylation of various tumor-suppressor genes, leading to their reactivation at mRNA and protein levels. The expression levels of various chromatin-modifying enzymes viz DNMTs, HMTs, HDACs, and HATS were found to be decreased, and H3 and H4 histone modification marks were modulated too. Also, reduced global DNA methylation was observed following the treatment of chrysin.Conclusion: This study concludes that chrysin can be used as a potential epigenetic modifier for cancer treatment and warrants for further experimental validation.
Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells
Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells
Other filter: ([btitle,keywords] "Epigenetic" OR [btit ...
Autiero I, Vitagliano L
* Enhanced molecular dynamic simulation studies unravel long-range effects caused by sequence variations and partner binding in RNA aptamers (31 views)
Mol Ther Nucleic Acids (ISSN: 2162-2531linking), 2023 Dec 12; 34: 102039-102039.
Impact Factor:
View Export to BibTeX Export to EndNote
Wang X, Martínez-fernández L, Zhang Y, Zhang K, Improta R, Kohler B, Xu J, Chen J
* Solvent-Dependent Stabilization of a Charge Transfer State is the Key to Ultrafast Triplet State Formation in an Epigenetic DNA Nucleoside (66 views)
Chemistry (ISSN: 0947-6539linking, 1521-3765electronic), 2021 Jul 26; 27(42): 10932-10940.
Impact Factor: 5.236
View Export to BibTeX Export to EndNote
Sundaram MK, Almutary AG, Alsulimani A, Ahmad SR, Somvanshi P, Bhardwaj T, Pellicano R, Fagoonee S, Hussain A, Haque S
* Antineoplastic action of sulforaphane on HeLa cells by modulation of signaling pathways and epigenetic pathways (59 views)
Hepatology (ISSN: 0026-4806linking, 1827-1669electronic), 2021 Jun 11; N/D: N/D-N/D.
Impact Factor: 4.806
View Export to BibTeX Export to EndNote
Gandhi S, Marr K, Mancini M, Caprio MG, Jakimovski D, Chandra A, Hagemeier J, Hojnacki D, Kolb C, Weinstock-guttman B, Zivadinov R
* No association between variations in extracranial venous anatomy and clinical outcomes in multiple sclerosis patients over 5 years (285 views)
Bmc Neurol (ISSN: 1471-2377linking, 1471-2377electronic), 2019 Jun 11; 19(1): 121-121.
Impact Factor: 2.17
View Export to BibTeX Export to EndNote
Diaferia C, Balasco N, Sibillano T, Giannini C, Vitagliano L, Morelli G, Accardo A
* Structural Characterization of Self-Assembled Tetra-Tryptophan Based Nanostructures: Variations on a Common Theme (173 views)
Chemphyschem (ISSN: 1439-4235linking, 1439-7641), 2018 Jul 5; 19 (13 ): 1635-1642.
Impact Factor: 3.502
View Export to BibTeX Export to EndNote
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L
* Combined HAT/EZH2 modulation leads to cancer-selective cell death (418 views)
Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646.
Impact Factor: 5.008
View Export to BibTeX Export to EndNote
Gullo D, Latina A, Frasca F, Squatrito S, Belfiore A, Vigneri R
* Seasonal variations in TSH serum levels in athyreotic patients under L-thyroxine replacement monotherapy (237 views)
Clin Endocrinol (ISSN: 0300-0664, 1365-2265, 0300-0664linking), 2017 Aug; 87(2): 207-215.
Impact Factor: 3.077
View Export to BibTeX Export to EndNote
Milite C, Castellano S, Benedetti R, Tosco A, Ciliberti C, Vicidomini C, Boully L, Franci G, Altucci L, Mai A, Sbardella G
* Modulation of the activity of histone acetyltransferases by long chain alkylidenemalonates (LoCAMs) (452 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 1464-3391electronic), 2011 Jun 15; 19(12): 3690-3701.
Impact Factor: 2.921
View Export to BibTeX Export to EndNote
Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Del Vecchio L, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M
* MicroRNA-199b-5p Impairs Cancer Stem Cells through Negative Regulation of HES1 in Medulloblastoma (516 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2009 Mar 24; 4(3): e4998-e4998.
Impact Factor: 4.351
View Export to BibTeX Export to EndNote
Chiechio S, Zammataro M, Morales ME, Busceti CL, Drago F, Th Gereau RW, Copani A, Nicoletti F
* Epigenetic modulation of mGlu2 receptors by histone deacetylase inhibitors in the treatment of inflammatory pain (826 views)
Mol Pharmacol (ISSN: 0026-895x), 2009; 75(5): 1014-1020.
Impact Factor: 4.531
View Export to BibTeX Export to EndNote
Sbardella G, Castellano S, Vicidomini C, Rotili D, Nebbioso A, Miceli M, Altucci L, Mai A
* Identification of long chain alkylidenemalonates as novel small molecule modulators of histone acetyltransferases (417 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2008 May 1; 18(9): 2788-2792.
Impact Factor: 2.531
View Export to BibTeX Export to EndNote
Clemente F, D'Avino E, Guaragno M, Menichetti A
* An on line technique to detect cardiac output variations and cardiovascular performances during abdominal aortic surgery (636 views)
Journal Of Clinical Monitoring And Computing (ISSN: 1387-1307), 2004 Apr; 18(2): 81-87.
Impact Factor: 0.888
View Export to BibTeX Export to EndNote
* Enhanced molecular dynamic simulation studies unravel long-range effects caused by sequence variations and partner binding in RNA aptamers (31 views)
Mol Ther Nucleic Acids (ISSN: 2162-2531linking), 2023 Dec 12; 34: 102039-102039.
Impact Factor:
View Export to BibTeX Export to EndNote
Wang X, Martínez-fernández L, Zhang Y, Zhang K, Improta R, Kohler B, Xu J, Chen J
* Solvent-Dependent Stabilization of a Charge Transfer State is the Key to Ultrafast Triplet State Formation in an Epigenetic DNA Nucleoside (66 views)
Chemistry (ISSN: 0947-6539linking, 1521-3765electronic), 2021 Jul 26; 27(42): 10932-10940.
Impact Factor: 5.236
View Export to BibTeX Export to EndNote
Sundaram MK, Almutary AG, Alsulimani A, Ahmad SR, Somvanshi P, Bhardwaj T, Pellicano R, Fagoonee S, Hussain A, Haque S
* Antineoplastic action of sulforaphane on HeLa cells by modulation of signaling pathways and epigenetic pathways (59 views)
Hepatology (ISSN: 0026-4806linking, 1827-1669electronic), 2021 Jun 11; N/D: N/D-N/D.
Impact Factor: 4.806
View Export to BibTeX Export to EndNote
Gandhi S, Marr K, Mancini M, Caprio MG, Jakimovski D, Chandra A, Hagemeier J, Hojnacki D, Kolb C, Weinstock-guttman B, Zivadinov R
* No association between variations in extracranial venous anatomy and clinical outcomes in multiple sclerosis patients over 5 years (285 views)
Bmc Neurol (ISSN: 1471-2377linking, 1471-2377electronic), 2019 Jun 11; 19(1): 121-121.
Impact Factor: 2.17
View Export to BibTeX Export to EndNote
Diaferia C, Balasco N, Sibillano T, Giannini C, Vitagliano L, Morelli G, Accardo A
* Structural Characterization of Self-Assembled Tetra-Tryptophan Based Nanostructures: Variations on a Common Theme (173 views)
Chemphyschem (ISSN: 1439-4235linking, 1439-7641), 2018 Jul 5; 19 (13 ): 1635-1642.
Impact Factor: 3.502
View Export to BibTeX Export to EndNote
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L
* Combined HAT/EZH2 modulation leads to cancer-selective cell death (418 views)
Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646.
Impact Factor: 5.008
View Export to BibTeX Export to EndNote
Gullo D, Latina A, Frasca F, Squatrito S, Belfiore A, Vigneri R
* Seasonal variations in TSH serum levels in athyreotic patients under L-thyroxine replacement monotherapy (237 views)
Clin Endocrinol (ISSN: 0300-0664, 1365-2265, 0300-0664linking), 2017 Aug; 87(2): 207-215.
Impact Factor: 3.077
View Export to BibTeX Export to EndNote
Milite C, Castellano S, Benedetti R, Tosco A, Ciliberti C, Vicidomini C, Boully L, Franci G, Altucci L, Mai A, Sbardella G
* Modulation of the activity of histone acetyltransferases by long chain alkylidenemalonates (LoCAMs) (452 views)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391, 1464-3391electronic), 2011 Jun 15; 19(12): 3690-3701.
Impact Factor: 2.921
View Export to BibTeX Export to EndNote
Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Del Vecchio L, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M
* MicroRNA-199b-5p Impairs Cancer Stem Cells through Negative Regulation of HES1 in Medulloblastoma (516 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2009 Mar 24; 4(3): e4998-e4998.
Impact Factor: 4.351
View Export to BibTeX Export to EndNote
Chiechio S, Zammataro M, Morales ME, Busceti CL, Drago F, Th Gereau RW, Copani A, Nicoletti F
* Epigenetic modulation of mGlu2 receptors by histone deacetylase inhibitors in the treatment of inflammatory pain (826 views)
Mol Pharmacol (ISSN: 0026-895x), 2009; 75(5): 1014-1020.
Impact Factor: 4.531
View Export to BibTeX Export to EndNote
Sbardella G, Castellano S, Vicidomini C, Rotili D, Nebbioso A, Miceli M, Altucci L, Mai A
* Identification of long chain alkylidenemalonates as novel small molecule modulators of histone acetyltransferases (417 views)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2008 May 1; 18(9): 2788-2792.
Impact Factor: 2.531
View Export to BibTeX Export to EndNote
Clemente F, D'Avino E, Guaragno M, Menichetti A
* An on line technique to detect cardiac output variations and cardiovascular performances during abdominal aortic surgery (636 views)
Journal Of Clinical Monitoring And Computing (ISSN: 1387-1307), 2004 Apr; 18(2): 81-87.
Impact Factor: 0.888
View Export to BibTeX Export to EndNote
12 Records (11 excluding Abstracts).
Total impact factor: 39.021 (34.67 excluding Abstracts).
Total 5 year impact factor: 37.839 (33.456 excluding Abstracts).
Total impact factor: 39.021 (34.67 excluding Abstracts).
Total 5 year impact factor: 37.839 (33.456 excluding Abstracts).
63 views. Last view on Monday 09 September 2024, 10:51:26
ibb.cnr.it
