Cell recruitment and transfection in gene activated collagen matrix (597 views)
Biomaterials (ISSN: 0142-9612, 1878-5905electronic, 0142-9612linking), 2010 Jan; 31(3): 570-576.
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Paper type: Journal Article,
Impact factor: 7.883, 5-year impact factor: 9.076
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-70449134628&partnerID=40&md5=9f08ebdda4ae3c1b8d880885059f6967
Keywords: Cell Migration, Cell Recruitment, Collagen, Dna Complexes Immobilization, Gene Activated Matrices, Non Viral Gene Delivery, Cell Transfection, Collagen Matrices, Control Of Gene Expression, Encoded Proteins, Gene-Activated Matrix, In-Vivo, New Approaches, Polyethylenimines, Time-Lapse Video Microscopy, Tissue Regeneration, Tissue Repair, Transfected Cells, Cytology, Employment, Fluorescence Microscopy, Gene Transfer, Nucleic Acids, Three Dimensional, Cell Immobilization, Avidin, Polyethyleneimine, Animal Cell, Article, Biotinylation, Cell Strain 3t3, Complex Formation, Conjugation, Controlled Study, Embryo, Fluorescence Activated Cell Sorting, Gene Activation, Gene Expression Regulation, Gene Targeting, Genetic Transfection, Internalization, Microenvironment, Mouse, Nonhuman, Priority Journal, Protein Modification, Cell Movement, Extracellular Matrix, Materials Testing, Molecular Structure, Nih 3t3 Cells, Spectrometry, Electrospray Ionization,
Affiliations:
*** IBB - CNR ***
Department of Materials and Production Engineering, University of Naples Federico II, Piazzale Tecchio 80, Naples, 80125, Italy
Interdisciplinary Research Centre on Biomaterials (CRIB), Italian Institute of Technology, IIT, Piazzale Tecchio 80, Naples, 80125, Italy
Department of Biological Science, section Biostructures, University of Naples Federico II, via Mezzocannone 16, Naples, 80134, Italy
References:
Not available.
Biomaterials (ISSN: 0142-9612, 1878-5905electronic, 0142-9612linking), 2010 Jan; 31(3): 570-576.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 7.883, 5-year impact factor: 9.076
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-70449134628&partnerID=40&md5=9f08ebdda4ae3c1b8d880885059f6967
Keywords: Cell Migration, Cell Recruitment, Collagen, Dna Complexes Immobilization, Gene Activated Matrices, Non Viral Gene Delivery, Cell Transfection, Collagen Matrices, Control Of Gene Expression, Encoded Proteins, Gene-Activated Matrix, In-Vivo, New Approaches, Polyethylenimines, Time-Lapse Video Microscopy, Tissue Regeneration, Tissue Repair, Transfected Cells, Cytology, Employment, Fluorescence Microscopy, Gene Transfer, Nucleic Acids, Three Dimensional, Cell Immobilization, Avidin, Polyethyleneimine, Animal Cell, Article, Biotinylation, Cell Strain 3t3, Complex Formation, Conjugation, Controlled Study, Embryo, Fluorescence Activated Cell Sorting, Gene Activation, Gene Expression Regulation, Gene Targeting, Genetic Transfection, Internalization, Microenvironment, Mouse, Nonhuman, Priority Journal, Protein Modification, Cell Movement, Extracellular Matrix, Materials Testing, Molecular Structure, Nih 3t3 Cells, Spectrometry, Electrospray Ionization,
Affiliations:
*** IBB - CNR ***
Department of Materials and Production Engineering, University of Naples Federico II, Piazzale Tecchio 80, Naples, 80125, Italy
Interdisciplinary Research Centre on Biomaterials (CRIB), Italian Institute of Technology, IIT, Piazzale Tecchio 80, Naples, 80125, Italy
Department of Biological Science, section Biostructures, University of Naples Federico II, via Mezzocannone 16, Naples, 80134, Italy
References:
Not available.
Cell recruitment and transfection in gene activated collagen matrix
Realization of systems able to both recruit cells and influence their fate (affecting their processes) represents a new approach for tissue regeneration. We investigated the potency of gene activated matrix (CAM) and implemented the CAM strategy in order to achieve a control of gene expression, as well as a specific cell recruitment. To this aim we developed a 3D DNA bio-activated collagen matrix by Poly (ethylenimine) (PEI)/DNA complex immobilization in the matrix through biotin/avidin bond. Moreover, we realised a serum based chemotactic gradient within the matrix in order to directionally attract NIH3T3 cells. In this system, cells are recruited and forced to migrate through the matrix where they find the bound PEI/DNA complexes and are transfected. The transfected cells can act as local in vivo bioreactors, secreting plasmid encoded proteins that augment tissue repair and regeneration. 3D cell migration and cell transfection were monitored through time-lapse video microscopy and fluorescence microscopy. Cell transfection was also quantified through FACS analysis. Results show that our engineered matrix is able to recruit external cells and transfect them once internalized, therefore it could help in tissue repairing strategy. (C) 2009 Elsevier Ltd. All rights reserved.
Realization of systems able to both recruit cells and influence their fate (affecting their processes) represents a new approach for tissue regeneration. We investigated the potency of gene activated matrix (CAM) and implemented the CAM strategy in order to achieve a control of gene expression, as well as a specific cell recruitment. To this aim we developed a 3D DNA bio-activated collagen matrix by Poly (ethylenimine) (PEI)/DNA complex immobilization in the matrix through biotin/avidin bond. Moreover, we realised a serum based chemotactic gradient within the matrix in order to directionally attract NIH3T3 cells. In this system, cells are recruited and forced to migrate through the matrix where they find the bound PEI/DNA complexes and are transfected. The transfected cells can act as local in vivo bioreactors, secreting plasmid encoded proteins that augment tissue repair and regeneration. 3D cell migration and cell transfection were monitored through time-lapse video microscopy and fluorescence microscopy. Cell transfection was also quantified through FACS analysis. Results show that our engineered matrix is able to recruit external cells and transfect them once internalized, therefore it could help in tissue repairing strategy. (C) 2009 Elsevier Ltd. All rights reserved.
Cell recruitment and transfection in gene activated collagen matrix
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Cell recruitment and transfection in gene activated collagen matrix
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Impact Factor: 2.76
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Neve Polimeno M, Ierano C, D'Alterio C, Simona Losito N, Napolitano M, Portella L, Scognamiglio G, Tatangelo F, Maria Trotta A, Curley S, Costantini S, Liuzzi R, Izzo F, Scala S
* CXCR4 expression affects overall survival of HCC patients whereas CXCR7 expression does not (840 views)
Cell Mol Immunol (ISSN: 1672-7681), 2014 Nov 3; 12(4): 474-482.
Impact Factor: 4.112
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Formisano L, Nappi L, Rosa R, Marciano R, D'Amato C, D'Amato V, Damiano V, Raimondo L, Iommelli F, Scorziello A, Troncone G, Veneziani BM, Parsons SJ, De Placido S, Bianco R
* Epidermal growth factor-receptor activation modulates Src-dependent resistance to lapatinib in breast cancer models (705 views)
Breast Cancer Res Biomed Central Ltd (ISSN: 1465-5411), 2014; 16(3): R45-R45.
Impact Factor: 0.635
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Portella L, Vitale R, De Luca S, D'Alterio C, Ieranò C, Napolitano M, Riccio A, Polimeno MN, Monfregola L, Barbieri A, Luciano A, Ciarmiello A, Arra C, Castello G, Amodeo P, Scala S
* Preclinical Development of a Novel Class of CXCR4 Antagonist Impairing Solid Tumors Growth and Metastases (617 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2013 Sep 13; 8(9): N/D-N/D.
Impact Factor: 3.534
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Romano S, Staibano S, Greco A, Brunetti A, Nappo G, Ilardi G, Martinelli R, Sorrentino A, Di Pace A, Mascolo M, Bisogni R, Scalvenzi M, Alfano B, Romano M
* FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential (659 views) (PDF 271 views)
Cell Death & (ISSN: 2041-4889, 2041-4889electronic), 2013 Apr 4; 4(6): e578-e578.
Impact Factor: 5.333
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Pisano M, De Paola I, Nieddu V, Sassu I, Cossu S, Galleri G, Del Gatto A, Budroni M, Cossu A, Saviano M, Palmieri G, Zaccaro L, Rozzo C
* In vitro activity of the alphavbeta3 integrin antagonist RGDechi-hCit on malignant melanoma cells (674 views)
Anticancer Res (ISSN: 0250-7005, 0250-0700, 1791-7530electronic), 2013 Mar; 33(3): 871-879.
Impact Factor: 1.045
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Galasso G, De Rosa R, Ciccarelli M, Sorriento D, Del Giudice C, Strisciuglio T, De Biase C, Luciano R, Piccolo R, Pierri A, Di Gioia G, Prevete N, Trimarco B, Piscione F, Iaccarino G
* β2-adrenergic receptor stimulation improves endothelial progenitor cell-mediated ischemic neoangiogenesis (585 views)
Circ Res (ISSN: 0009-7330, 1524-4571electronic, 0009-7330linking), 2013; 112(7): 1026-1034.
Impact Factor: 11.089
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Finetti F, Basile A, Capasso D, Di Gaetano S, Di Stasi R, Pascale M, Turco CM, Ziche M, Morbidelli L, D'Andrea LD
* Functional and pharmacological characterization of a VEGF mimetic peptide on reparative angiogenesis (740 views)
Biochem Pharmacol Biochemical Pharmacology (ISSN: 0006-2952, 1873-2968), 2012 Sep 1; 84(3): 303-311.
Impact Factor: 4.576
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Guarnieri D, De Capua A, Ventre M, Borzacchiello A, Pedone C, Marasco D, Ruvo M, Netti PA
* Covalently immobilized RGD gradient on PEG hydrogel scaffold influences cell migration parameters (792 views)
Acta Biomaterialia (ISSN: 1878-7568, 1742-7061), 2010 Jul; 6(7): 2532-2539.
Impact Factor: 4.824
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Carriero MV, Longanesi-cattani I, Bifulco K, Maglio O, Lista L, Barbieri A, Votta G, Masucci MT, Arra C, Franco R, De Rosa M, Stoppelli MP, Pavone V
* Structure-based design of an urokinase-type plasminogen activator receptor-derived peptide inhibiting cell migration and lung metastasis (702 views)
Mol Cancer Ther (ISSN: 1535-7163, 1538-8514electronic, 1535-7163linking), 2009 Sep; 8(9): 2708-2717.
Impact Factor: 4.953
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D'Andrea LD, Del Gatto A, De Rosa L, Romanelli A, Pedone C
* Peptides targeting angiogenesis related growth factor receptors (14836 views)
Curr Pharm Des Current Pharmaceutical Design (ISSN: 1873-4286, 1381-6128), 2009 Jul; 15(21): 2414-2429.
Impact Factor: 4.414
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Ierano C, Giuliano P, D'Alterio C, Cioffi M, Mettivier V, Portella L, Napolitano M, Barbieri A, Arra C, Liguori G, Franco R, Palmieri G, Rozzo C, Pacelli R, Castello G, Scala S
* A point mutation (G574A) in the chemokine receptor CXCR4 detected in human cancer cells enhances migration (472 views)
Cell Cycle (ISSN: 1538-4101), 2009 Apr 15; 8(8): 1228-1237.
Impact Factor: 4.087
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Pavone V, Lista L, Maglio O, Longanesi-cattani I, Bifulco K, Arra C, V , Carriero M
* Sub-femtomolar activity of a new cell migration inhibitor (358 views)
Biopolymers, 2009; 92: 350-350.
Impact Factor: 0
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Gioia M, Monaco S, Van Den Steen PE, Sbardella D, Grasso G, Marini S, Overall CM, Opdenakker G, Coletta M
* The Collagen Binding Domain of Gelatinase A Modulates Degradation of Collagen IV by Gelatinase (523 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2009; 386(2): 419-434.
Impact Factor: 3.871
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Bifulco K, Longanesi-cattani I, Gargiulo L, Maglio O, Cataldi M, De Rosa M, Stoppelli MP, Pavone V, Carriero MV
* An urokinase receptor antagonist that inhibits cell migration by blocking the formyl peptide receptor (508 views)
Febs Lett (ISSN: 0014-5793print, 0014-5793linking, 1873-3468electronic), 2008 Apr 2; 582(7): 1141-1146.
Impact Factor: 3.264
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Guarnieri D, Borzacchiello A, De Capua A, Marasco D, Ruvo M, Netti PA
* Dependence of cell migration parameters on steepness of RGD gradient slope (474 views)
World Biomater Congr (ISSN: 9781-6156), 2008; 1: 271-271.
Impact Factor: 0
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Sorriento D, Ciccarelli M, Santulli G, Campanile A, Altobelli GG, Cimini V, Galasso G, Astone D, Piscione F, Pastore L, Trimarco B, Iaccarino G
* The G-protein-coupled receptor kinase 5 inhibits NFκB transcriptional activity by inducing nuclear accumulation of IκBα (688 views)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2008; 105(46): 17818-17823.
Impact Factor: 9.38
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Bucci EM, Valente M, Musumeci D, Sapio R, Anrò V, Roviello GN, Moccia M
* ODN-based drugs for targeting of extracellular proteins (429 views)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1525-7770), 2007; 26(8-9): 1047-1050.
Impact Factor: 0.723
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D'Andrea LD, Del Gatto A, Pedone C, Benedetti E
* Peptide-based molecules in angiogenesis (1005 views)
Chem Biol Drug Des (ISSN: 1747-0277, 1747-0285, 1747-0277linking), 2006 Feb; 67(2): 115-126.
Impact Factor: 2.507
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Zannetti A, Del Vecchio S, Romanelli A, Scala S, Saviano M, Cali' G, Stoppelli MP, Pedone C, Salvatore M
* Inhibition of Sp1 activity by a decoy PNA-DNA chimera prevents urokinase receptor expression and migration of breast cancer cells (657 views)
Biochem Pharmacol Biochemical Pharmacology (ISSN: 0006-2952, 1873-2968), 2005 Nov 1; 70(9): 1277-1287.
Impact Factor: 3.617
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Carriero M, Del Vecchio S, Capozzoli M, Franco P, Fontana L, Zannetti A, Botti G, D'Aiuto G, Salvatore M, Stoppelli M
Urokinase receptor interacts with alpha(v)beta(5) vitronectin receptor, promoting urokinase-dependent cell migration in breast cancer (487 views)
Cancer Res (ISSN: 0008-5472, 1538-7445, 1538-7445electronic), 1999 Oct 15; 59(20): N/D-N/D.
Impact Factor: 8.614
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34 Records (28 excluding Abstracts).
Total impact factor: 155.356 (137.24 excluding Abstracts).
Total 5 year impact factor: 133.48 (115.521 excluding Abstracts).
Total impact factor: 155.356 (137.24 excluding Abstracts).
Total 5 year impact factor: 133.48 (115.521 excluding Abstracts).
597 views. Last view on Tuesday 10 December 2024, 9:58:21
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