Biochemical Characterisation Of The D60a Mutant Of The Elongation Factor 1 Alpha From The Archaeon Sulfolobus Solfataricus(307 views) Ruggiero I, Cantiello P, Lamberti A, Sorrentino A, Martucci NM, Ruggiero A, Arcone R, Vitagliano L, Arcari P, Masullo M
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, I-80131 Napoli, Italy
Dipartimento di Scienze Farmacobiologiche, Università degli Studi Magna Graecia di Catanzaro, Complesso Ninì Barbieri, I-88021 Roccelletta di Borgia, CZ, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134 Napoli, Italy
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Krab, I. M., Parmeggiani, A., Mechanisms of EF-Tu, a pioneer GTPase (2002) Prog. Nucleic Acid Res. Mol. Biol., 71, pp. 513-551
Andersen, G. R., Pedersen, L., Valente, L., Chatterjee, I., Kinzy, T. G., Kjeldgaard, M., Nyborg, J., Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A: eEF1B (2000) Mol. Cell, 6, pp. 1261-1266
Andersen, G. R., Thirup, S., Spremulli, L. L., Nyborg, J., High resolution crystal structure of bovine mitochondrial EF-Tu in complex with GDP (2000) J. Mol. Biol., 297, pp. 421-436
Gaucher, E. A., Thomson, J. M., Burgan, M. F., Benner, S. A., Inferring the palaeo environment of ancient bacteria on the basis of resurrected proteins (2003) Nature, 425, pp. 285-288
Pittman, Y. R., Valente, L., Jeppesen, M. G., Andersen, G. R., Patel, S., Kinzy, T. G., Mg2+ and a key lysine modulate exchange activity of eukaryotic translation elongation factor 1B (2006) J. Biol. Chem., 281, pp. 19457-19468
Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
Kraulis, P. J., MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures (1991) J. Appl. Crystallogr., 24, pp. 946-950
Hutchinson, E. G., Thornton, J. M., PROMOTIF a program to identify and analyze structural motifs in proteins (1996) Protein Sci., 5, pp. 212-220
Heffron, S. E., Mui, S., Aorora, A., Abel, K., Bergmann, E., Jurnak, F., Molecular complementarity between tetracycline and the GTPase active site of elongation factor Tu (2006) Acta Crystallogr. D Biol. Crystallogr., 62, pp. 1392-1400
Biochemical Characterisation Of The D60a Mutant Of The Elongation Factor 1 Alpha From The Archaeon Sulfolobus Solfataricus