Keywords: Insulin Degrading Enzyme, Mass Spectrometry, Noncovalent Interaction, Surface Plasmon Resonance, Ubiquitin, Insulinase, Article, Enzyme Activity, Enzyme Binding, Enzyme Degradation, Priority Journal, Protein Degradation, Protein Function, Animals, Insulysin, Protein Binding, Recombinant Proteins, Matrix-Assisted Laser Desorption-Ionization, Spodoptera,
Affiliations: *** IBB - CNR ***
Chemistry Department, Universita di Catania, Viale Andrea Doria 6, 95125, Catania, Italy.
Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, Catania, Italy
References: Farris, W., Mansourian, S., Chang, Y., Lindsley, L., Eckman, E.A., Frosch, M.P., Eckman, C.B., Guenette, S., Insulin-degrading enzyme regulates the levels of insulin, amyloid β-protein, and the β-amyloid precursor protein intracellular domain in vivo (2003) Proc. Natl. Acad. Sci. U. S. A., 100, pp. 4162-416
Shen, Y., Joachimiak, A., Rosner, M.R., Tang, W.-J., Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism (2006) Nature, 443, pp. 870-874
Soto, C., Unfolding the role of protein misfolding in neurodegenerative diseases (2003) Nat. Rev. Neurosci., 4, pp. 49-60
Chiti, F., Dobson, C.M., Protein misfolding, functional amyloid, and human disease (2006) Annu. Rev. Biochem., 75, pp. 333-366
Bertram, L., Blacker, D., Mullin, K., Keeney, D., Jones, J., Basu, S., Yhu, S., Tanzi, R.E., Evidence for genetic linkage of Alzheimer's disease to chromosome 10q (2000) Science, 290, pp. 2302-2303
Qiu, W.Q., Folstein, M.F., Insulin, insulin-degrading enzyme and amyloid-b peptide in Alzheimer's disease: review and hypothesis (2006) Neurobiol. aging, 27, pp. 190-198
Bennett, R.G., Hamel, F.G., Duckworth, W.C., Characterization of the insulin inhibition of the peptidolytic activities of the insulin-degrading enzyme-proteasome complex (1997) Diabetes, 46, pp. 197-203
Saric, T., Muller, D., Seitz, H.-J., Pavelic, K., Non-covalent interaction of ubiquitin with insulin-degrading enzyme (2003) Mol. Cell. Endocrinol., 204, pp. 11-20
Duckworth, W.C., Bennett, R.G., Hamel, F.G., Insulin acts intracellularly on proteasomes through insulin-degrading enzyme (1998) Biochem. Biophys. Res. Commun., 244, pp. 390-394
Bennett, E.J., Shaler, T.A., Woodman, B., Ryu, K.-Y., Zaitseva, T.S., Becker, C.H., Bates, G.P., Kopito, R.R., Global changes to the ubiquitin system in Huntington's disease (2007) Nature, 448, pp. 704-709
Ross, C.A., Pickart, C.M., The ubiquitin-proteasome pathway in Parkinson's disease and other neurodegenerative diseases (2004) Trends Cell Biol., 14, pp. 703-711
Lam, Y.A., Pickart, C.M., Alban, A., Landon, M., Jamieson, C., Ramage, R., Mayer, R.J., Layfield, R., Inhibition of the ubiquitin-proteasome system in Alzheimer's disease (2000) Proc. Natl. Acad. Sci. U. S. A., 97, pp. 9902-9906
Grasso, G., Rizzarelli, E., Spoto, G., AP/MALDI-MS complete characterization of insulin fragments produced by the interaction of IDE with bovine insulin (2007) J. Mass Spectrom., 42, pp. 1590-1598
Grasso, G., D'Agata, R., Rizzarelli, E., Spoto, G., D'Andrea, L., Pedone, C., Picardi, A., Yeo, K.J., Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au (111) surfaces monitored by ESI-MS (2005) J. Mass Spectrom., 40, pp. 1565-1571
Shevchenko, A., Shevchenko, A., Evaluation of the efficiency of in-gel digestion of proteins by peptide isotopic labeling and MALDI mass spectrometry (2001) Anal. Biochem., 296, pp. 279-283
Hershko, A., Ciechanover, A., The ubiquitin system (1998) Annu. Rev. Biochem., 67, pp. 425-479
Qiu, W. Q., Folstein, M. F., Insulin, insulin-degrading enzyme and amyloid-b peptide in Alzheimer's disease: review and hypothesis (2006) Neurobiol. aging, 27, pp. 190-198
Bennett, R. G., Hamel, F. G., Duckworth, W. C., Characterization of the insulin inhibition of the peptidolytic activities of the insulin-degrading enzyme-proteasome complex (1997) Diabetes, 46, pp. 197-203
Duckworth, W. C., Bennett, R. G., Hamel, F. G., Insulin acts intracellularly on proteasomes through insulin-degrading enzyme (1998) Biochem. Biophys. Res. Commun., 244, pp. 390-394
Bennett, E. J., Shaler, T. A., Woodman, B., Ryu, K. -Y., Zaitseva, T. S., Becker, C. H., Bates, G. P., Kopito, R. R., Global changes to the ubiquitin system in Huntington's disease (2007) Nature, 448, pp. 704-709
Ross, C. A., Pickart, C. M., The ubiquitin-proteasome pathway in Parkinson's disease and other neurodegenerative diseases (2004) Trends Cell Biol., 14, pp. 703-711
Lam, Y. A., Pickart, C. M., Alban, A., Landon, M., Jamieson, C., Ramage, R., Mayer, R. J., Layfield, R., Inhibition of the ubiquitin-proteasome system in Alzheimer's disease (2000) Proc. Natl. Acad. Sci. U. S. A., 97, pp. 9902-9906
How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin
Insulin degrading enzyme (IDE) is known to play a pivotal role on amyloidogenic peptide degradation but little is known about the changes in the proteolytic activity of the enzyme upon modification of external factors. Particularly, although it has been reported that altered ubiquitin concentration and/or hyperinsulinaemia increase the risk of developing Alzheimer's disease (AD), the molecular mechanism involved is unclear. In this work, we study the role that ubiquitin plays on IDE capability of binding and degrading insulin molecules and the obtained results indicate that ubiquitin has an allosteric role for IDE and high ubiquitin levels impair IDE activity.
How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin