Pathophysiological changes of gram-negative bacterial infection can be reproduced by a synthetic peptide mimicking loop L7 sequence of Haemophilus influenzae porin
Pathophysiological changes of gram-negative bacterial infection can be reproduced by a synthetic peptide mimicking loop L7 sequence of Haemophilus influenzae porin(323 views) Vitiello M, Galdiero S, D'Isanto M, D'Amico M, Di Filippo C, Cantisani M, Galdiero M, Pedone C
Department of Experimental Medicine-II University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
Department of Biological Sciences, Division of Biostructures - University of Naples Federico II, Istituto di Biostrutture, Via Mezzocannone 16, 80134 Naples, Italy
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Huntley, J. F., Conley, P. G., Hagman, K. E., Norgard, M. V., Characterization of Francisella tularensis outer membrane proteins (2007) J. Bacteriol., 189, pp. 561-574
Weiss, M. S., Schulz, G. E., Structure of porin refined at 1. 8 A resolution (1992) J. Mol. Biol., 227, pp. 493-509
Yi, K., Murphy, T. F., Importance of an immunodominant surface exposed loop on outer membrane protein P2 of nontypeable Haemophilus influenzae (1997) Infect. Immun., 65, pp. 150-155
Neary, J. M., Yi, K., Karalus, R. J., Murphy, T. F., Antibodies to loop 6 of the P2 porin protein of nontypeable Haemophilus influenzae are bactericidal against multiple strains (2001) Infect. Immun., 69, pp. 773-778
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Opal, S. M., Gluck, T., Endotoxin as a drug target (2003) Crit. Care Med., 31, pp. 57-64
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Aird, W. C., The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome (2003) Blood, 101, pp. 3765-3777
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 277, pp. 680-685
Tsai, C. M., Frasch, C. E., A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels (1982) Anal. Biochem., 119, pp. 115-119
Yin, E. T., Galanos, C., Kinsky, S., A Bradshaw, R., Wessler, S., Luderity, O., Sarmiento, M. F., Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria (1972) Biochim. Biophys. Acta, 261, pp. 284-289
Issekutz, A. C., Removal of gram-negative endotoxin from solutions by affinity chromatography (1983) J. Immunol. Methods, 61, pp. 275-281
H lm, S., A simple sequentially rejective multiple test procedure (1979) Scand. J. Statist., 6, pp. 65-70
Wang, Y. X., Dong, N., Wu, C., Martin-McNulty, B., Fitch, R. M., da Cunha, V., Vicelette, J., Wu, Q., Lipopolysaccharide attenuates thrombolysis in batroxobin-induced lung vasculature fibrin deposition but not in ferrous chloride-induced carotid artery thrombus in rats: role of endogenous PAI-1 (2003) Thromb. Res., 111, pp. 381-387
Schweda, E. K. H., Richards, J. C., Hood, D. W., Moxon, E. R., Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence (2007) Int. J. Med. Microbiol., 297, pp. 297-306
Shen, K. P., Lo, Y. C., Yang, R. C., Liu, H. W., Chen, I. J., Wu, B. N., Antioxidanteugenosedin-A protects against lipopolysaccharide-induced hypotension, hyperglycaemia and cytokine immunoreactivity in rats and mice (2005) J. Pharm. Pharmacol., 57, pp. 117-125
Pathophysiological changes of gram-negative bacterial infection can be reproduced by a synthetic peptide mimicking loop L7 sequence of Haemophilus influenzae porin
Several in vivo models have been used to dissect the molecular mechanisms that contribute to activate the coagulation and fibrinolytic systems by bacteria and bacterial products but many aspects remain poorly understood. In this study we examined the in vivo effect of the synthetic peptide corresponding to loop L7 from Haemophilus influenzae type b (Hib) porin to evaluate its role on the coagulative/fibrinolytic cascade and the circulating markers of endothelial injury. Plasma was obtained from rats injected intravenously with loop L7, Hib porin or a scrambled peptide and tested for fragment 1+2 (F1+2), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor type I (PAI-1) antigen, von Willebrand factor (vWF) and soluble E-selectin (sE-selectin). The coagulative/fibrinolytic cascade was impaired as shown by PAI-1 level increased. Concomitantly, E-selectin, a marker of endothelial injury, was also significantly elevated. In addition either loop L7 or Hib porin injection induced hyperglycaemia and inflammatory cytokine production. The data were correlated with hemodynamic functions. The results indicate that loop L7 plays an essential role in the pathophysiologic events observed during gram-negative infection. These findings may have implications for the development of alternative therapies to counteract excessive inflammatory responses during septic shock.
Pathophysiological changes of gram-negative bacterial infection can be reproduced by a synthetic peptide mimicking loop L7 sequence of Haemophilus influenzae porin
Pathophysiological changes of gram-negative bacterial infection can be reproduced by a synthetic peptide mimicking loop L7 sequence of Haemophilus influenzae porin
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Testino G, Leone S, Fagoonee S, Del Bas JM, Rodriguez B, Puiggros F, Marine S, Rodriguez MA, Morina D, Armengol L, Caimari A, Arola L, Cimini FA, Barchetta I, Carotti S, Bertoccini L, Baroni MG, Vespasiani-gentilucci U, Cavallo MG, Morini S, Nelson JE, Roth CL, Wilson LA, Yates KP, Aouizerat B, Morgan-stevenson V, Whalen E, Hoofnagle A, Mason M, Gersuk V, Yeh MM, Kowdley KV, Lee SM, Jun DW, Cho YK, Jang KS, Kucukazman M, Ata N, Dal K, Yeniova AO, Kefeli A, Basyigit S, Aktas B, Akin KO, Agladioglu K, Ure OS, Topal F, Nazligul Y, Beyan E, Ertugrul DT, Catena C, Cosma C, Camozzi V, Plebani M, Ermani M, Sechi LA, Fallo F, Goto Y, Ray MB, Mendenhall CL, French SW, Gartside PS Serum vitamin A deficiency and increased intrahepatic expression of cytokeratin antigen in alcoholic liver disease(600 views) Hepatology (ISSN: 1827-1669electronic, 0026-4806linking), 1988 Sep; 83120693611123109(5): 1019-1026. Impact Factor:0.913 ViewExport to BibTeXExport to EndNote
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