Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin (681 views)
Biol Chem Biological Chemistry (ISSN: 1431-6730, 1437-4315), 2008 Nov; 389(11): 1421-1426.
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Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.035, 5-year impact factor: 2.945
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-56549094997&partnerID=40&md5=0c8758d42e3bb9a3e15bcb4f813332a3
Keywords: Apoa-I Zaragoza, Apoa-I Zavalla, High-Density Lipoprotein, Lecithin-Cholesterol Acyltransferase, Peptides, Reverse Cholesterol Transport, Apolipoprotein A1, Haptoglobin, High Density Lipoprotein Cholesterol, Phosphatidylcholine Sterol Acyltransferase, Amino Acid Substitution, Article, Binding Assay, Binding Site, Correlation Analysis, Enzyme Activity, Enzyme Regulation, Human, Point Mutation, Priority Journal, Protein Binding, Protein Protein Interaction, Protein Variant, Amino Acid Sequence, Competitive, Molecular Sequence Data, Phosphatidylcholine-Sterol O-Acyltransferase,
Affiliations:
*** IBB - CNR ***
Dipartimento delle Scienze Biologiche, Universita di Napoli Federico II, I-80134 Napoli, Italy.
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, I-80134 Napoli, Italy
Dipartimento di Patologia Sistematica, Sezione di Dermatologia, Università di Napoli Federico II, I-80131 Napoli, Italy
Istituto Per Il Sistema Produzione Animale in Ambiente Mediterraneo, Consiglio Nazionale Delle Ricerche, I-80147 Napoli, Italy
Istituto di Genetica e Biofisica 'A. Buzzati Traverso', Consiglio Nazionale Delle Ricerche, I-80131 Napoli, Italy
References:
Not available.
Biol Chem Biological Chemistry (ISSN: 1431-6730, 1437-4315), 2008 Nov; 389(11): 1421-1426.
Export to BibTeX Export to EndNote
Paper type: Journal Article, Research Support, Non-U. S. Gov'T,
Impact factor: 3.035, 5-year impact factor: 2.945
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-56549094997&partnerID=40&md5=0c8758d42e3bb9a3e15bcb4f813332a3
Keywords: Apoa-I Zaragoza, Apoa-I Zavalla, High-Density Lipoprotein, Lecithin-Cholesterol Acyltransferase, Peptides, Reverse Cholesterol Transport, Apolipoprotein A1, Haptoglobin, High Density Lipoprotein Cholesterol, Phosphatidylcholine Sterol Acyltransferase, Amino Acid Substitution, Article, Binding Assay, Binding Site, Correlation Analysis, Enzyme Activity, Enzyme Regulation, Human, Point Mutation, Priority Journal, Protein Binding, Protein Protein Interaction, Protein Variant, Amino Acid Sequence, Competitive, Molecular Sequence Data, Phosphatidylcholine-Sterol O-Acyltransferase,
Affiliations:
*** IBB - CNR ***
Dipartimento delle Scienze Biologiche, Universita di Napoli Federico II, I-80134 Napoli, Italy.
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale Delle Ricerche, I-80134 Napoli, Italy
Dipartimento di Patologia Sistematica, Sezione di Dermatologia, Università di Napoli Federico II, I-80131 Napoli, Italy
Istituto Per Il Sistema Produzione Animale in Ambiente Mediterraneo, Consiglio Nazionale Delle Ricerche, I-80147 Napoli, Italy
Istituto di Genetica e Biofisica 'A. Buzzati Traverso', Consiglio Nazionale Delle Ricerche, I-80131 Napoli, Italy
References:
Not available.
Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin
The high-density lipoprotein apolipoprotein A-I (ApoA-I) stimulates the enzyme lecithin-cholesterol acyltransferase (LCAT) in the reverse cholesterol transport pathway. Two ApoA-I variants, Zaragoza (L144R) and Zavalla (L159P), are associated with low levels of HDL-cholesterol but normal LCAT activity. Haptoglobin interacts with ApoA-I, impairing LCAT stimulation. Synthetic peptides matching the haptoglobin-binding site of native or variant ApoA-I (native, P2a; variants, Zav-pep and Zar-pep) bound haptoglobin with different activity: Zar-pep>P2a>Zav-pep. They also differently rescued LCAT in vitro activity in the presence of haptoglobin (P2a=Zar-pep>Zav-pep). Therefore, both amino acid conversions affect haptoglobin binding and LCAT regulation. We highlight the role of haptoglobin in LCAT regulation in subjects with ApoA-I variants. © 2008 by Walter de Gruyter.
The high-density lipoprotein apolipoprotein A-I (ApoA-I) stimulates the enzyme lecithin-cholesterol acyltransferase (LCAT) in the reverse cholesterol transport pathway. Two ApoA-I variants, Zaragoza (L144R) and Zavalla (L159P), are associated with low levels of HDL-cholesterol but normal LCAT activity. Haptoglobin interacts with ApoA-I, impairing LCAT stimulation. Synthetic peptides matching the haptoglobin-binding site of native or variant ApoA-I (native, P2a; variants, Zav-pep and Zar-pep) bound haptoglobin with different activity: Zar-pep>P2a>Zav-pep. They also differently rescued LCAT in vitro activity in the presence of haptoglobin (P2a=Zar-pep>Zav-pep). Therefore, both amino acid conversions affect haptoglobin binding and LCAT regulation. We highlight the role of haptoglobin in LCAT regulation in subjects with ApoA-I variants. © 2008 by Walter de Gruyter.
Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin
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Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin
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Cigliano L, Pugliese CR, Spagnuolo MS, Palumbo R, Abrescia P
* Haptoglobin binds the antiatherogenic protein apolipoprotein e - Impairment of apolipoprotein e stimulation of both lecithin: Cholesterol acyltransferase activity and cholesterol uptake by hepatocytes (747 views)
Febs J (ISSN: 1742-464x), 2009 Nov; 276(21): 6158-6171.
Impact Factor: 3.042
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Cigliano L, Maresca B, Salvatore A, Nino M, Monfrecola G, Ayala F, Carlucci A, Pugliese RC, Pedone C, Abrescia P
* Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity (729 views)
Journal Of The European Academy Of Dermatology And Venereology (ISSN: 1468-3083, 0926-9959), 2008 Apr; 22(4): 417-425.
Impact Factor: 2.276
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* Haptoglobin binds the antiatherogenic protein apolipoprotein e - Impairment of apolipoprotein e stimulation of both lecithin: Cholesterol acyltransferase activity and cholesterol uptake by hepatocytes (747 views)
Febs J (ISSN: 1742-464x), 2009 Nov; 276(21): 6158-6171.
Impact Factor: 3.042
View Export to BibTeX Export to EndNote
Cigliano L, Maresca B, Salvatore A, Nino M, Monfrecola G, Ayala F, Carlucci A, Pugliese RC, Pedone C, Abrescia P
* Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity (729 views)
Journal Of The European Academy Of Dermatology And Venereology (ISSN: 1468-3083, 0926-9959), 2008 Apr; 22(4): 417-425.
Impact Factor: 2.276
View Export to BibTeX Export to EndNote
2 Records (2 excluding Abstracts).
Total impact factor: 5.318 (5.318 excluding Abstracts).
Total 5 year impact factor: 5.333 (5.333 excluding Abstracts).
Total impact factor: 5.318 (5.318 excluding Abstracts).
Total 5 year impact factor: 5.333 (5.333 excluding Abstracts).
681 views. Last view on Tuesday 15 April 2025, 17:20:39
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