Keywords: Biosensing Techniques, Dna, Peptide Nucleic Acids, Surface Plasmon Resonance, Analytic Method, Biotinylation, Chemical Labeling, Dna Determination, Dna Rna Hybridization, Electrochemical Detection, Gene Probe, Genetic Procedures, Human, Nucleic Acid Analysis, Review, Rna Analysis, Sensitivity And Specificity, Sequence Analysis, Single Nucleotide Polymorphism,
Affiliations: *** IBB - CNR ***
Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy
Institute of Biostructures and Bioimages, CNR, Catania, Italy
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Lee, H. J., Wark, A. W., Corn, R. M., Creating Advanced Multifunctional Biosensors with Surface Enzymatic Transformations (2006) Langmuir, 22, pp. 5241-5250
Rothenh usler, B., Knoll, W., Surface plasmon microscopy (1988) Nature, 332, pp. 615-617
Jensen, K. K., rum, H., Nielsen, P. E., Norden, B., Kinetics for Hybridization of Peptide Nucleic Acids (PNA) with DNA and RNA Studied with the BIAcore Technique (1997) Biochemistry, 36, pp. 5072-5077
Burgener, M., S nger, M., Candrian, U., Synthesis of a stable and specific surface plasmon resonance biosensor surface employing covalently immobilized peptide nucleic acids (2000) Bioconjugate Chem, 11, pp. 749-754
Goodrich, T. T., Lee, H. J., Corn, R. M., Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA MICROARRAYS (2004) J Am Chem Soc, 126, pp. 4086-4087
Fang, S., Lee, H. J., Wark, A. W., Corn, R. M., Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions (2006) J Am Chem Soc, 128, pp. 14044-14046
He, L., Musick, M. D., Nicewarner, S. R., Salinas, F. G., Benkovic, S. J., Natan, M. J., Colloidal au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization (2000) J Am Chem Soc, 122, pp. 9071-9077
Su, X., Teh, H. F., Aung, K. M. M., Zong, Y., Gao, Z., Femtomol SPR detection of DNA - DNA hybridization with the assistance of DNA-guided polyaniline deposition (2008) Biosens Bioelectron, 23, pp. 1715-1720
Li, Y., Wark, A. W., Lee, H. J., Corn, R. M., Single-nucleotide polymorphism genotyping by nanoparticle-enhanced surface plasmon resonance imaging measurements of surface ligation reactions (2006) Anal Chem, 78, pp. 3158-3164
Peterlinz, K. A., Herne, T. M., Tarlov, M. J., Georgiadis, R. M., Observation of hybridization and dehybridization of thiol-tethered DNA using two-color surface plasmon resonance spectroscopy (1997) J Am Chem Soc, 119, pp. 3401-3402
Peterson, A. W., Heaton, R. J., Georgiadis, R., Kinetic control of hybridization in surface immobilized DNA monolayer films (2000) J Am Chem Soc, 122, pp. 7837-7838
Feller, B. E., Kellis Jr, J. T., Casc o-Pereira, L. G., Knoll, W., Robertson, C. R., Frank, C. W., Fluorescence Quantification for Surface Plasmon Excitation (2008) Langmuir, 24, pp. 12303-12311
Chu, L. Q., F rch, R., Knoll, W., Surface-plasmon-enhanced fluorescence spectroscopy for DNA detection using fluorescently labeled PNA as "DNA indicator (2007) Angew Chem Int Ed, 46, pp. 4944-4947
Surface plasmon-based optical detection of DNA peptide nucleic acids
Surface plasmon-based optical methods using peptide nucleic acids (PNAs) probes have recently emerged as powerful and highly sensitive tools for DNA and RNA detection. Surface plasmon resonance (SPR) is a powerful technique for the high sensitive and label-free detection of nucleic acids while PNAs help in targeting complementary sequences with exceptionally high selectivity and sensitivity. The present review summarizes the research efforts paid over the last years in the above mentioned field. In particular, fundamental aspects of the SPR detection of the PNA/DNA hybridization will be discussed. Then, research activities aimed at demonstrating the ultrasensitive DNA detection and the possibilities offered in single nucleotide polymorphisms detection will be presented. The contribution given by surface plasmon-allied techniques and by the combined use of the electrochemical detection will be also discussed. The SPR detection of DNA by chiral PNAs will be finally mentioned.
Surface plasmon-based optical detection of DNA peptide nucleic acids