Keywords: Analogue, Backbone, Linker, Nucleobase, Peptide Nucleic Acid, Synthesis, Nucleic Acid Base, Oligonucleotide, Dna Modification, Gene Expression, Genetic Analysis, In Vivo Study, Protein Structure, Review, Animals, Humans, Nucleic Acid Conformation, Protein Binding, Protein Conformation,
Affiliations: *** IBB - CNR ***
Università degli Studi di Napoli 'Federico II', Dipartimento Delle Scienze Biologiche, Facoltà di Scienze Biotecnologiche, 80134 Napoli, Italy
Istituto di Biostrutture e Bioimmagini CNR, via Mezzocannone 16, 80134 Napoli, Italy
Universit degli Studi di Napoli 'Federico II', Dipartimento Delle Scienze Biologiche, Facolt di Scienze Biotecnologiche, 80134 Napoli, Italy
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New peptide nucleic acid analogues: synthesis and applications
Peptide nucleic acids are oligonucleotide mimics characterised by high chemical and enzymatic stability, high specificity and affinity toward complementary DNA/RNA. The lack of charge and polar groups in the backbone decrease their solubility in aqueous environment and their ability to cross cell membranes, reducing their performance in in vivo applications. To improve solubility, increase affinity and specificity of binding and to control recognition between nucleic acids, several analogues bearing modifications on the nucleobase, nucleobase-backbone linker and on the backbone were synthesised. This paper describes the synthesis and applications of Peptide nucleic acid analogues and discusses the potential of analogues for which no application is reported.
New peptide nucleic acid analogues: synthesis and applications