A new spot test for sulfhydryl-containing compounds(509 views) Cappiello M, Alterio V, Amodeo P, Del Corso A, Scaloni A, Pedone C, Moschini R, De Donatis GM, De Simone G, Mura U
Institute of Biological Chemistry University of Roma, 00185 Roma, Italy
Department of Physiology and Biochemistry, University of Pisa, I-56126 Pisa, Italy
Institute of Biostructures and Bioimages, CNR, I-80134 Naples, Italy
Institute of Biomolecular Chemistry, CNR, Comprensorio Olivetti, I-80078 Pozzuoli (Naples), Italy
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, I-80147 Naples, Italy
References: Not available.
A new spot test for sulfhydryl-containing compounds
Bovine lens leucyl aminopeptidase (blLAP), a homohexameric metallopeptidase preferring bulky and hydrophobic amino acids at the N-terminus of (di) peptides, contains two Zn2+ ions per subunit that are essential for catalytic activity. They may be replaced by other divalent cations with different exchange kinetics. The protein readily exchangeable site (site 1) can be occupied by Zn2+, Mn2+, Mg2+, or Co2+, while the tight binding site (site 2) can be occupied by Zn2+ or Co 2+. We recently reported that introduction of Mn2+ into site 1 generates a novel activity of blLAP toward CysGly [Cappiello, M., et al. (2004) Biochem. J. 378, 35-44], which in contrast is not hydrolyzed by the (Zn/Zn) enzyme. This finding, while disclosing a potential specific role for blLAP in glutathione metabolism, raised a question about the features required for molecules to be a substrate for the enzyme. To clarify the interaction of the enzyme with sulfhydryl-containing derivatives, (Zn/Zn) - and (Mn/Zn) blLAP forms were prepared and functional-structural studies were undertaken. Thus, a kinetic analysis of various compounds with both enzyme forms was performed; the crystal structure of (Zn/Zn) blLAP in complex with the peptidomimetic derivative Zofenoprilat was determined, and a modeling study on the CysGly- (Zn/Zn) blLAP complex was carried out. This combined approach provided insight into the interaction of blLAP with sulfhydryl-containing derivatives, showing that the metal exchange in site 1 modulates binding to these molecules that may result in enzyme substrates or inhibitors, depending on the nature of the metal. 2006 American Chemical Society
A new spot test for sulfhydryl-containing compounds
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A new spot test for sulfhydryl-containing compounds
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(532 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(562 views) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 ViewExport to BibTeXExport to EndNote