Interactions of the cytotoxic RNase A dimers with the cytosolic ribonuclease inhibitor(362 views) Naddeo M, Vitagliano L, Russo A, Gotte G, D'Alessio G, Sorrentino S
Febs Lett (ISSN: 0014-5793, 0014-5793print, 1873-3468electronic), 2005 May 9; 579(12): 2663-2668.
Keywords: Antitumor Activity, Domain Swapping, Protein Dimerization, Ribonuclease A, Ribonuclease Cytotoxicity, Ribonuclease Inhibitor, Esterase Inhibitor, Unclassified Drug, Antineoplastic Activity, Article, Complex Formation, Concentration Response, Cytosol, Drug Mechanism, Drug Protein Binding, Enzyme Activity, Enzyme Inhibition, Priority Journal, Animals, Cattle, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Pancreatic, Spectrophotometry,
Affiliations: *** IBB - CNR ***
Dipto. Biol. Strutturale Funzionale, Univ. di Napoli Federico II, Via Mezzocannone 16, I-80134 Napoli, Italy
Ist. di Biostrutture e Bioimmagini, CNR, CIRPEB, Via Mezzocannone 16, I-80134 Napoli, Italy
Dipartimento di Scienze Della Vita, Seconda Università di Napoli, Via Vivaldi 43, I-81110 Caserta, Italy
Dipto. Sci. Neurologiche Della V., Università di Verona, Strada Le Grazie 8, 37134 Verona, Italy
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Leland, P. A., Schultz, L. W., Kim, B. -L., Raines, R. T., Ribonuclease a variants with potent cytotoxic activity (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 10407-10412
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Youle, R. J., D'Alessio, G., (1997) Ribonucleases: Structures and Function, pp. 491-514. , G. D'Alessio J. F. Riordan Academic Press San Diego
Monti, D. M., D'Alessio, G., Cytosolic RNase inhibitor only affects RNases with intrinsic cytotoxicity (2004) J. Biol. Chem., 279, pp. 39195-39198
Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
Jones, T. A., Zou, J. -Y., Cowan, S. W., Kjeldgaard, M., Improved methods for the building of protein models in electron density maps and the location of errors in these (1991) Acta Crystallogr., 47, pp. 110-119
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Newton, D. L., Xue, Y., Boque, L., Wlodawer, A., Kung, H. F., Rybak, S. M., Expression and characterization of a cytotoxic human-frog chimeric ribonuclease: Potential for cancer therapy (1997) Protein Eng., 10, pp. 463-470
Kraulis, P. J., Molscript - A program to produce both detailed and schematic plots of protein structures (1991) J. Appl. Crystallogr., 24, pp. 945-949
Merritt, E. A., Murphy, M. E. P., RASTER3D version 2. 0: A program for photorealistic molecular graphics (1994) Acta Crystallogr., 50, pp. 869-873
Interactions of the cytotoxic RNase A dimers with the cytosolic ribonuclease inhibitor
Ribonuclease A (RNase A) dimers have been recently found to be endowed with some of the special, i.e., non-catalytic biological activities of RNases, such as antitumor and aspermatogenic activities. These activities have been so far attributed to RNases which can escape the neutralizing action of the cytosolic RNase inhibitor (cRI). However, when the interactions of the two cytotoxic RNase A dimers with cRI were investigated in a quantitative fashion and at the molecular level, the dimers were found to bind cRI with high affinity and to form tight complexes. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Interactions of the cytotoxic RNase A dimers with the cytosolic ribonuclease inhibitor
No results.
Interactions of the cytotoxic RNase A dimers with the cytosolic ribonuclease inhibitor