An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius(326 views) Bartolucci S, De Simone G, Galdiero S, Improta R, Menchise V, Pedone C, Pedone E, Saviano M
Dipartimento di Chimica Biologica, University of Naples Federico II, 80134 Naples, Italy
Ist. Biostrutture e Bioimmagini-CNR, 80134 Naples, Italy
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Pace, C. N., Alston, R. W., Shaw, K. L., Charge-charge interactions influence the denatured state ensemble and contribute to protein stability (2000) Protein Sci., 9, pp. 1395-1398
Pace, C. N., Single surface stabilizer (2000) Nat. Struct. Biol., 7, pp. 345-346
Petsko, G. A., Structural basis of thermostability in hyperthermophilic proteins, or "there's more than one way to skin a cat" (2001) Methods Enzymol., 334, pp. 469-478
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Szil gyi, A., Z vodszky, P., Structural differences between mesophilic, moderately thermophilic and extremely thermophilic proteins subunits: Results of a comprehensive survey (2000) Structure, 8, pp. 493-504
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An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius
We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.
An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius
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Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(449 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote