Hyaluronate tetrasaccharide-Cu(II) interaction: A NMR study(441 views) D'Auria G, Flores G, Falcigno L, Oliva R, Vacatello M, Corsaro MM, Parrilli M, Paolillo L
Keywords: Binding Sites, Carbohydrate Sequence, Carbon Chemistry, Copper Chemistry, Glucuronic Acid Chemistry, Hyaluronic Acid Chemistry, Ions, Magnetic Resonance Spectroscopy, Models, Molecular, Monosaccharides Chemistry, Oxygen Chemistry, Polysaccharides Chemistry, Protons, Cu(ii), Paramagnetic Relaxation, Chemical Relaxation, Coordination Reactions, Magnetic Field Effects, Nuclear Magnetic Resonance, Paramagnetic Materials, Metal Ions, Carboxylic Acid, Coordination Compound, Ligand, Tetrasaccharide, Unclassified Drug, Aqueous Solution, Article, Carbon Nuclear Magnetic Resonance, Molecular Interaction, Molecular Model, Proton Nuclear Magnetic Resonance, Cu (ii),
Affiliations: *** IBB - CNR ***
Department of Chemistry, University Federico II of Naples, 80126 Naples, Italy.
Inst. Biostructure/Bioimaging of CNR, 80134 Naples, Italy
Dept. of Organ. Chem. and Biochem., University Federico II of Naples, 80126 Naples, Italy
References: Not available.
Hyaluronate tetrasaccharide-Cu(II) interaction: A NMR study
The coordination of Cu(II) to a hyaluronate tetrasaccharide (HA(t)) was investigated in aqueous solution by C-13 and H-1 relaxation measurements at two magnetic fields, 9 and 14 T The HA(t) interaction with the metal ion was monitored following the nuclear paramagnetic relaxation enhancements R-1p and R-2p produced by the copper addition. The data analysis shows that the paramagnetic effect is differently experienced by the nuclei in different monosaccharide residues. A molecular model for the complex HA(t)-Cu(II) was built taking into account the experimental data. The model shows the presence of two binding sites, both involving the carboxylate groups of the two glucuronic acid units. The first site, that best simulates the HA binding site, is located on the ligand core, while the second one is located on the terminal glucuronic acid residue. Both binding sites involve, in addition to the carboxylate groups, the O4 oxygens of the glucuronic acid residues. (C) 2003 Wiley Periodicals, Inc.
Hyaluronate tetrasaccharide-Cu(II) interaction: A NMR study