Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide Aβ1-42 by mirror image phage display(709 views) Wiesehan K, Buder K, Linke RP, Patt S, Stoldt M, Unger E, Schmitt B, Bucci E, Willbold D
Glenner, G.G., Henry, J.H., Fujihara, S., (1981) Ann. Pathol., 1, pp. 120-129
Linke, R.P., (2000) Virchows Arch., 436, pp. 439-448
Serpell, L. C., (2000) Biochim. Biophys. Acta, 1502, pp. 16-30
Fox, N. C., Crum, W. R., Scahill, R. I., Stevens, J. M., Janssen, J. C., Rossor, M. N., (2001) Lancet, 358, pp. 201-205
Dezutter, N. A., Dom, R. J., De Groot, T. J., Bormans, G. M., Verbruggen, A. M., (1999) Eur. J. Nucl. Med., 26, pp. 1392-1399
Klunk, W. E., Wang, Y., Huang, G. F., Debnath, M. L., Holt, D. P., Mathis, C. A., (2001) Life Sci., 69, pp. 1471-1484
Skovronsky, D. M., Zhang, B., Kung, M. P., Kung, H. F., Trojanowski, J. Q., Lee, V. M., (2000) Proc. Natl. Acad. Sci. U. S. A., 97, pp. 7609-7614
Agdeppa, E. D., Kepe, V., Liu, J., Flores-Torres, S., Satyamurthy, N., Petric, A., Cole, G. M., Barrio, J. R., (2001) J. Neurosci., 21, pp. RC189
Wengenack, T. M., Curran, G. L., Poduslo, J. F., (2000) Nat. Biotechnol., 18, pp. 868-872
Smith, G. P., (1985) Science, 228, pp. 1315-1317
Mattheakis, L. C., Bhatt, R. R., Dower, W. J., (1994) Proc. Natl. Acad. Sci. U. S. A., 91, pp. 9022-9026
Hanes, J., Pl ckthun, A., (1997) Proc. Natl. Acad. Sci. U. S. A., 94, pp. 4937-4942
Schumacher, T. N., Mayr, L. M., Minor D. L., Jr., Milhollen, M. A., Burgess, M. W., Kim, P. S., (1996) Science, 271, pp. 1854-1857
Zawadzke, L. E., Berg, J. M., (1992) J. Am. Chem. Soc., 114, pp. 4002-4003
Poduslo, J. F., Curran, G. L., Kumar, A., Frangione, B., Soto, C., (1999) J. Neurobiol., 39, pp. 371-382
Findeis, M. A., Musso, G. M., Arico-Muendel, C. C., Benjamin, H. W., Hundal, A. M., Lee, J. J., Chin, J., Molineaux, S. M., (1999) Biochemistry, 38, pp. 6791-6800
Dintzis, H. M., Symer, D. E., Dintzis, R. Z., Zawadzke, L. E., Berg, J. M., (1993) Proteins, 16, pp. 306-308
Robson, S. C., Young, V. K., Cook, N. S., Metternich, R., Konig, W. K., Lesnikoski, B. A., Pierson R. N. III, Bach, F. H., (1996) Transplantation, 61, pp. 862-868
Eckert, D. M., Malashkevich, V. N., Hong, L. H., Carr, P. A., Kim, P. S., (1999) Cell, 99, pp. 103-115
Kozlov, I. A., Mao, S., Xu, Y., Huang, X., Lee, L., Sears, P. S., Gao, C., Wong, C. H., (2001) ChemBioChem, 2, pp. 741-746
Selkoe, D. J., (1999) Nature, 399, pp. A23-31
M ller, B., Restle, T., Reinstein, J., Goody, R. S., (1991) Biochemistry, 30, pp. 3709-3715
Klunk, W. E., Jacob, R. F., Mason, R. P., (1999) Anal. Biochem., 266, pp. 66-76
Schnell, S. A., Staines, W. A., Wessendorf, M. W. J., (1999) Histochem. Cytochem., 47, pp. 719-730
Glenner, G. G., Henry, J. H., Fujihara, S., (1981) Ann. Pathol., 1, pp. 120-129
Linke, R. P., (2000) Virchows Arch., 436, pp. 439-448
Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide Aβ1-42 by mirror image phage display
A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Aβ(1-42). A randomized, 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Aβ(1-42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D-pep) was shown to bind Aβ(1-42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing Aβ amyloid were stained specifically with a fluorescence-labeled derivative of D-pep. Fibrillar deposits derived from other amyloidosis were not labeled by D-pep. Possible applications of this novel and highly specific Aβ ligand in diagnosis and therapy of Alzheimer's disease are discussed.
Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide Aβ1-42 by mirror image phage display