Crystallization of the collagen-like polypeptide (PPG)(10) aboard the International Space Station. 1. Video observation (496 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2002 Oct; 58: 1690-1694.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.76, 5-year impact factor: 9.416
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036793496&partnerID=40&md5=e79d94d9e72c7924fcd5976441fb7b47
Keywords: Advanced Protein Crystallization Facility, Crystallogenesis, Microgravity, (prolyl Prolyl Glycine)10, (prolyl-Prolyl-Glycine)10, Collagen, Peptide, Sepharose, Chemistry, Comparative Study, Conference Paper, Flight Experiment, Iss Project, Long Duration, Manned, Methodology, Solution And Solubility, Space Flight, Videorecording, Weightlessness, Time Factors, Video Recording, (prolyl Prolyl Glycine) 10, (prolyl-Prolyl-Glycine) 10,
Affiliations:
*** IBB - CNR ***
Dipartimento di Chimica Biologica, Universita degli Studi di Napoli, Via Mezzocannone 6, Napoli, Italy
References:
Not available.
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2002 Oct; 58: 1690-1694.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.76, 5-year impact factor: 9.416
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036793496&partnerID=40&md5=e79d94d9e72c7924fcd5976441fb7b47
Keywords: Advanced Protein Crystallization Facility, Crystallogenesis, Microgravity, (prolyl Prolyl Glycine)10, (prolyl-Prolyl-Glycine)10, Collagen, Peptide, Sepharose, Chemistry, Comparative Study, Conference Paper, Flight Experiment, Iss Project, Long Duration, Manned, Methodology, Solution And Solubility, Space Flight, Videorecording, Weightlessness, Time Factors, Video Recording, (prolyl Prolyl Glycine) 10, (prolyl-Prolyl-Glycine) 10,
Affiliations:
*** IBB - CNR ***
Dipartimento di Chimica Biologica, Universita degli Studi di Napoli, Via Mezzocannone 6, Napoli, Italy
References:
Not available.
Crystallization of the collagen-like polypeptide (PPG)(10) aboard the International Space Station. 1. Video observation
Single chains of the collagen model polypeptide with sequence (Pro-Pro-Gly)(10), hereafter referred to as (PPG)(10), aggregate to form rod-shaped triple helices. Crystals of (PPG)(10) were grown in the Advanced Protein Crystallization Facility (APCF) both onboard the International Space Station (ISS) and on Earth. The experiments allow the direct comparison of four different crystallization environments for the first time: solution in microgravity (mug), agarose gel in mug, solution on earth, and gel on earth. Both on board and on ground, the crystal growth was monitored by a CCD video camera. The image analysis provided information on the spatial distribution of the crystals, their movement and their growth rate. The analysis of the distribution of crystals reveals that the crystallization process occurs as it does in batch conditions. Slow motions have been observed onboard the ISS. Different to Space-Shuttle experiment, the crystals onboard the ISS moved coherently and followed parallel trajectories. Growth rate and induction time are very similar both in gel and in solution, suggesting that the crystal growth rate is controlled by the kinetics at the interface under the used experimental conditions. These results provide the first data in the crystallogenesis of (PPG)(10), which is a representative member of non-globular, rod-like proteins.
Single chains of the collagen model polypeptide with sequence (Pro-Pro-Gly)(10), hereafter referred to as (PPG)(10), aggregate to form rod-shaped triple helices. Crystals of (PPG)(10) were grown in the Advanced Protein Crystallization Facility (APCF) both onboard the International Space Station (ISS) and on Earth. The experiments allow the direct comparison of four different crystallization environments for the first time: solution in microgravity (mug), agarose gel in mug, solution on earth, and gel on earth. Both on board and on ground, the crystal growth was monitored by a CCD video camera. The image analysis provided information on the spatial distribution of the crystals, their movement and their growth rate. The analysis of the distribution of crystals reveals that the crystallization process occurs as it does in batch conditions. Slow motions have been observed onboard the ISS. Different to Space-Shuttle experiment, the crystals onboard the ISS moved coherently and followed parallel trajectories. Growth rate and induction time are very similar both in gel and in solution, suggesting that the crystal growth rate is controlled by the kinetics at the interface under the used experimental conditions. These results provide the first data in the crystallogenesis of (PPG)(10), which is a representative member of non-globular, rod-like proteins.
Crystallization of the collagen-like polypeptide (PPG)(10) aboard the International Space Station. 1. Video observation
No results. |
Crystallization of the collagen-like polypeptide (PPG)(10) aboard the International Space Station. 1. Video observation
Other filter: ([btitle,keywords] "Advanced Protein Cry ...
Kelly SE, Di Benedetto A, Greco A, Howard CM, Sollars VE, Primerano DA, Valluri JV, Claudio PP
* Rapid Selection and Proliferation of CD133(+) Cells from Cancer Cell Lines: Chemotherapeutic Implications (649 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2010 Apr 8; 5(4): N/D-N/D.
Impact Factor: 4.411
View Export to BibTeX Export to EndNote
Vergara A, Castagnolo D, Carotenuto L, Vitagliano L, Berisio R, Sorrentino G, Gonzalez-ramirez L, Garcia-ruiz J, Zagari A
* Phase behavior and crystallogenesis under counter-diffusion conditions of the collagen-model peptide (Pro-Pro-Gly)10 (674 views)
J Cryst Growth (ISSN: 0022-0248), 2009 Jan 1; 311(2): 304-309.
Impact Factor: 1.534
View Export to BibTeX Export to EndNote
Vergara A, Lorber B, Sauter C, Giege R, Zagari A
* Lessons from crystals grown in the Advanced Protein Crystallisation Facility for conventional crystallisation applied to structural biology (501 views)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2005; 118(2-3): 102-112.
Impact Factor: 1.925
View Export to BibTeX Export to EndNote
Castagnolo D, Piccolo C, Carotenuto L, Vergara A, Zagari A
* Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 3. Analysis of residual acceleration-induced motion (649 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2003; 59(4): 773-776.
Impact Factor: 2.208
View Export to BibTeX Export to EndNote
Vergara A, Lorber B, Zagari A, Giege R
* Physical aspects of protein crystal growth investigated with the advanced protein crystallization facility in reduced-gravity environments (788 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2003; 59(1): 2-15.
Impact Factor: 2.208
View Export to BibTeX Export to EndNote
Berisio R, Vitagliano L, Vergara A, Sorrentino G, Mazzarella L, Zagari A
* Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction (588 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2002 Oct; 58(10II): 1695-1699.
Impact Factor: 1.76
View Export to BibTeX Export to EndNote
Carotenuto L, Cartwright J, Otálora F, Garcia-ruiz J, Castagnolo D, Piccolo C, Berisio R, Vitagliano L, Mazzarella L, Zagari A
* Depletion Zone Around Sedimenting Protein Crystals In Microgravity (367 views)
Esa Journal, 2001; 454: 323-329.
Impact Factor: 0.895
View Export to BibTeX Export to EndNote
Carotenuto L, Berisio R, Piccolo C, Vitagliano L, Zagari A
* Video observation of protein crystal growth in the advanced protein crystallization facility aboard the space shuttle mission STS-95 (527 views)
J Cryst Growth (ISSN: 0022-0248), 2001; 232(1-4): 481-488.
Impact Factor: 1.283
View Export to BibTeX Export to EndNote
Berisio R, Vitagliano L, Sorrentino G, Carotenuto L, Piccolo C, Mazzarella L, Zagari A
Effects of microgravity on the crystal quality of a collagen-like polypeptide (844 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2000 Jan; 56(1): 55-61.
Impact Factor: 3.067
View Export to BibTeX Export to EndNote
Carotenuto L, Castagnolo D, Piccolo C, Berisio R, Vitagliano L, Mazzarella L, Zagari A
Biomolecule crystallisation in microgravity (453 views)
Eur Space Agency Spec Publ Esa Sp (ISSN: 0379-6566), 2000; (454I): 437-443.
Impact Factor: 3.445
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia C, Rossi M, Lamzin VS, Willson KS, Zagari A
Protein Crystal Growth In The Advance Protein Crystallisation Facility On The Lms Mission: A Comparison Of Sulfolobus Solfataricus Alchool Dehydrogenase Crystals Grown On The Ground And In Microgravity (407 views)
Acta Crystallogr D Biol Crystallogr, 1998; 54: 386-390.
Impact Factor: 0
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia CA, Rossi M, Lamzin VS, Wilson KS, Zagari A
Protein crystal growth in the advanced protein crystallization facility on the LMS mission: A comparison of Sulfolobus solfataricus alcohol dehydrogenase crystals grown on the ground and in microgravity (699 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 1998; 54(3): 386-390.
Impact Factor: 2.244
View Export to BibTeX Export to EndNote
* Rapid Selection and Proliferation of CD133(+) Cells from Cancer Cell Lines: Chemotherapeutic Implications (649 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2010 Apr 8; 5(4): N/D-N/D.
Impact Factor: 4.411
View Export to BibTeX Export to EndNote
Vergara A, Castagnolo D, Carotenuto L, Vitagliano L, Berisio R, Sorrentino G, Gonzalez-ramirez L, Garcia-ruiz J, Zagari A
* Phase behavior and crystallogenesis under counter-diffusion conditions of the collagen-model peptide (Pro-Pro-Gly)10 (674 views)
J Cryst Growth (ISSN: 0022-0248), 2009 Jan 1; 311(2): 304-309.
Impact Factor: 1.534
View Export to BibTeX Export to EndNote
Vergara A, Lorber B, Sauter C, Giege R, Zagari A
* Lessons from crystals grown in the Advanced Protein Crystallisation Facility for conventional crystallisation applied to structural biology (501 views)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2005; 118(2-3): 102-112.
Impact Factor: 1.925
View Export to BibTeX Export to EndNote
Castagnolo D, Piccolo C, Carotenuto L, Vergara A, Zagari A
* Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 3. Analysis of residual acceleration-induced motion (649 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2003; 59(4): 773-776.
Impact Factor: 2.208
View Export to BibTeX Export to EndNote
Vergara A, Lorber B, Zagari A, Giege R
* Physical aspects of protein crystal growth investigated with the advanced protein crystallization facility in reduced-gravity environments (788 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2003; 59(1): 2-15.
Impact Factor: 2.208
View Export to BibTeX Export to EndNote
Berisio R, Vitagliano L, Vergara A, Sorrentino G, Mazzarella L, Zagari A
* Crystallization of the collagen-like polypeptide (PPG)10 aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction (588 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2002 Oct; 58(10II): 1695-1699.
Impact Factor: 1.76
View Export to BibTeX Export to EndNote
Carotenuto L, Cartwright J, Otálora F, Garcia-ruiz J, Castagnolo D, Piccolo C, Berisio R, Vitagliano L, Mazzarella L, Zagari A
* Depletion Zone Around Sedimenting Protein Crystals In Microgravity (367 views)
Esa Journal, 2001; 454: 323-329.
Impact Factor: 0.895
View Export to BibTeX Export to EndNote
Carotenuto L, Berisio R, Piccolo C, Vitagliano L, Zagari A
* Video observation of protein crystal growth in the advanced protein crystallization facility aboard the space shuttle mission STS-95 (527 views)
J Cryst Growth (ISSN: 0022-0248), 2001; 232(1-4): 481-488.
Impact Factor: 1.283
View Export to BibTeX Export to EndNote
Berisio R, Vitagliano L, Sorrentino G, Carotenuto L, Piccolo C, Mazzarella L, Zagari A
Effects of microgravity on the crystal quality of a collagen-like polypeptide (844 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2000 Jan; 56(1): 55-61.
Impact Factor: 3.067
View Export to BibTeX Export to EndNote
Carotenuto L, Castagnolo D, Piccolo C, Berisio R, Vitagliano L, Mazzarella L, Zagari A
Biomolecule crystallisation in microgravity (453 views)
Eur Space Agency Spec Publ Esa Sp (ISSN: 0379-6566), 2000; (454I): 437-443.
Impact Factor: 3.445
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia C, Rossi M, Lamzin VS, Willson KS, Zagari A
Protein Crystal Growth In The Advance Protein Crystallisation Facility On The Lms Mission: A Comparison Of Sulfolobus Solfataricus Alchool Dehydrogenase Crystals Grown On The Ground And In Microgravity (407 views)
Acta Crystallogr D Biol Crystallogr, 1998; 54: 386-390.
Impact Factor: 0
View Export to BibTeX Export to EndNote
Esposito L, Sica F, Sorrentino G, Berisio R, Carotenuto L, Giordano A, Raia CA, Rossi M, Lamzin VS, Wilson KS, Zagari A
Protein crystal growth in the advanced protein crystallization facility on the LMS mission: A comparison of Sulfolobus solfataricus alcohol dehydrogenase crystals grown on the ground and in microgravity (699 views)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 1998; 54(3): 386-390.
Impact Factor: 2.244
View Export to BibTeX Export to EndNote
12 Records (11 excluding Abstracts).
Total impact factor: 24.98 (20.569 excluding Abstracts).
Total 5 year impact factor: 54.331 (49.721 excluding Abstracts).
Total impact factor: 24.98 (20.569 excluding Abstracts).
Total 5 year impact factor: 54.331 (49.721 excluding Abstracts).
496 views. Last view on Sunday 08 December 2024, 0:58:25
ibb.cnr.it
