Hyperpigmentation induced by topical 5-aminolaevulinic acid plus visible light (495 views)
J Photochem Photobiol B (ISSN: 1011-1344, 1011-1344print, 1011-1344linking), 2002 Nov; 68(2-3): 147-155.
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Paper type: Journal Article,
Impact factor: 1.573, 5-year impact factor: 2.892
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036866519&partnerID=40&md5=5344360a81e8162ed9babd7ac889871d
Keywords: 5-Aminolaevulinic Acid, Melanogenesis, Photodynamic Therapy, Visible Light, Aminolevulinic Acid, Photosensitizing Agent, Protoporphyrin, Adult, Article, Colorimetry, Concentration (parameters), Condyloma, Controlled Study, Cream, Drug Effect, Drug Formulation, Drug Penetration, Electron Microscopy, Erythema, Female, Fluorescence Analysis, Histopathology, Human, Human Experiment, Human Tissue, Hyperpigmentation, Immunohistochemistry, In Vivo Study, Irradiation, Melanocyte, Normal Human, Priority Journal, Protective Clothing, Psoriasis, Radiation Dose, Skin Cancer, Skin Discoloration, Skin Edema, Skin Penetration, Verruca Vulgaris, Chemically Induced Disorder, Middle Aged, Photochemotherapy, Radiation Exposure, Topical Drug Administration, Ultrastructure,
Affiliations:
*** IBB - CNR ***
Department of Dermatology, University Federico II, Via S. Pansini 5, 80131 Napoli, Italy
Dept. of Biomorphol./Function. Sci., Section of Pathology, University Federico II, Napoli, Italy
Department of Physic Science, Nat'l. Inst. of Fisica della Materia, Napoli, Italy
References:
Not available.
J Photochem Photobiol B (ISSN: 1011-1344, 1011-1344print, 1011-1344linking), 2002 Nov; 68(2-3): 147-155.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.573, 5-year impact factor: 2.892
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0036866519&partnerID=40&md5=5344360a81e8162ed9babd7ac889871d
Keywords: 5-Aminolaevulinic Acid, Melanogenesis, Photodynamic Therapy, Visible Light, Aminolevulinic Acid, Photosensitizing Agent, Protoporphyrin, Adult, Article, Colorimetry, Concentration (parameters), Condyloma, Controlled Study, Cream, Drug Effect, Drug Formulation, Drug Penetration, Electron Microscopy, Erythema, Female, Fluorescence Analysis, Histopathology, Human, Human Experiment, Human Tissue, Hyperpigmentation, Immunohistochemistry, In Vivo Study, Irradiation, Melanocyte, Normal Human, Priority Journal, Protective Clothing, Psoriasis, Radiation Dose, Skin Cancer, Skin Discoloration, Skin Edema, Skin Penetration, Verruca Vulgaris, Chemically Induced Disorder, Middle Aged, Photochemotherapy, Radiation Exposure, Topical Drug Administration, Ultrastructure,
Affiliations:
*** IBB - CNR ***
Department of Dermatology, University Federico II, Via S. Pansini 5, 80131 Napoli, Italy
Dept. of Biomorphol./Function. Sci., Section of Pathology, University Federico II, Napoli, Italy
Department of Physic Science, Nat'l. Inst. of Fisica della Materia, Napoli, Italy
References:
Not available.
Hyperpigmentation induced by topical 5-aminolaevulinic acid plus visible light
Photodynamic therapy (PDT) with 5-aminolaevulinic acid (ALA) is an alternative tool for the treatment of superficial non-melanoma skin cancers. Recently ALA-PDT has been employed with encouraging results also for warts, condylomata and psoriasis. In this study the effects of topical ALA plus irradiation with visible light on intact human skin have been evaluated. Five skin areas (A, B, C, D, and E) on the inner upper part of the arms of five healthy volunteers (skin types III and IV) were treated with (A) ALA 20% in base cream without irradiation, (B) only the vehicle (base cream) without ALA, (C, D and E) ALA cream at the concentrations of 5, 10 and 20%, respectively; all treatments were applied with an occlusive dressing. Four hours after ALA or vehicle application areas B, C, D and E were irradiated with a fixed dose of 40 J/cm2. ALA penetration through the intact skin was evaluated by in vivo fluorescence determination. The effects on healthy skin were evaluated by clinical and chromometric examinations, light microscopy, immunohistochemistry and electron microscopy. Results: (1) in vivo fluorescence demonstrated that ALA is able to penetrate through the intact skin, when applied with occlusive dressing and induces a classical fluorescence peak due to Protoporphyrin IX (PpIX) formation, which is the active photosensitiser. (2) Skin areas receiving ALA plus irradiation showed erythema and swelling just after the irradiative session and hyperpigmentation 48-72 h later. (3) Colourimetric data confirmed significant skin colour changes: values a* (representing the erythematous changes) increased only on the skin areas where ALA+irradiation were applied and during the 48 h after irradiation, thereafter a* began to decrease; values L* (pigmentation) increased during the 2 weeks following treatment. (4) Histopathological, immunohistochemical (S100, HMB-45) and electron microscopic findings showed an absolute increment of the number of melanocytes, which appeared clearly activated. In conclusion the application of ALA cream followed by irradiation is able to induce a pigmentation response in healthy human skin, at least in skin types III and IV. This melanocytic activation could have a potential for the treatment of skin disorders characterised by hypopigmentation. © 2002 Elsevier Science B.V. All rights reserved.
Photodynamic therapy (PDT) with 5-aminolaevulinic acid (ALA) is an alternative tool for the treatment of superficial non-melanoma skin cancers. Recently ALA-PDT has been employed with encouraging results also for warts, condylomata and psoriasis. In this study the effects of topical ALA plus irradiation with visible light on intact human skin have been evaluated. Five skin areas (A, B, C, D, and E) on the inner upper part of the arms of five healthy volunteers (skin types III and IV) were treated with (A) ALA 20% in base cream without irradiation, (B) only the vehicle (base cream) without ALA, (C, D and E) ALA cream at the concentrations of 5, 10 and 20%, respectively; all treatments were applied with an occlusive dressing. Four hours after ALA or vehicle application areas B, C, D and E were irradiated with a fixed dose of 40 J/cm2. ALA penetration through the intact skin was evaluated by in vivo fluorescence determination. The effects on healthy skin were evaluated by clinical and chromometric examinations, light microscopy, immunohistochemistry and electron microscopy. Results: (1) in vivo fluorescence demonstrated that ALA is able to penetrate through the intact skin, when applied with occlusive dressing and induces a classical fluorescence peak due to Protoporphyrin IX (PpIX) formation, which is the active photosensitiser. (2) Skin areas receiving ALA plus irradiation showed erythema and swelling just after the irradiative session and hyperpigmentation 48-72 h later. (3) Colourimetric data confirmed significant skin colour changes: values a* (representing the erythematous changes) increased only on the skin areas where ALA+irradiation were applied and during the 48 h after irradiation, thereafter a* began to decrease; values L* (pigmentation) increased during the 2 weeks following treatment. (4) Histopathological, immunohistochemical (S100, HMB-45) and electron microscopic findings showed an absolute increment of the number of melanocytes, which appeared clearly activated. In conclusion the application of ALA cream followed by irradiation is able to induce a pigmentation response in healthy human skin, at least in skin types III and IV. This melanocytic activation could have a potential for the treatment of skin disorders characterised by hypopigmentation. © 2002 Elsevier Science B.V. All rights reserved.
Hyperpigmentation induced by topical 5-aminolaevulinic acid plus visible light
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Hyperpigmentation induced by topical 5-aminolaevulinic acid plus visible light
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* Selective Photo-Assisted Eradication of Triple-Negative Breast Cancer Cells through Aptamer Decoration of Doped Conjugated Polymer Nanoparticles (38 views)
Pharmaceutics (ISSN: 1999-4923linking, 1999-4923electronic), 2022 Mar 12; 14(3): N/D-N/D.
Impact Factor: 6.321
View Export to BibTeX Export to EndNote
Guaragna A, Roviello GN, D'Errico S, Paolella C, Palumbo G, D'Alonzo D
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Impact Factor: 2.347
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FOLEY S, JONES G, LAND E J, LIUZZI R, MCGARVEY D, PERRY M, USCOTT T G
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Impact Factor: 1.918
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3 Records (3 excluding Abstracts).
Total impact factor: 10.586 (10.586 excluding Abstracts).
Total 5 year impact factor: 2.212 (2.212 excluding Abstracts).
Total impact factor: 10.586 (10.586 excluding Abstracts).
Total 5 year impact factor: 2.212 (2.212 excluding Abstracts).
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