Molecular organization of the cullin E3 ligase adaptor KCTD11 (435 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Apr; 93(4): 715-724.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.022, 5-year impact factor: 3.424
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-79952438310&partnerID=40&md5=ae8de93cd49681613dc1846115c0b865
Keywords: Hedgehog Pathway, Light Scattering, Medulloblastoma, Protein-Protein Interactions, Structure-Function Relationships, Ubiquitination, Adaptor Protein, Cullin3, Kctd11, Ligase, Potassium Channel Tetramerization Domain 11, Unclassified Drug, Article, Biological Activity, Controlled Study, Escherichia Coli, Human, Human Cell, Nonhuman, Protein Binding, Protein Domain, Protein Expression, Protein Variant, Amino Acid Sequence, Base Sequence, Cullin Proteins, Hek293 Cells, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Isoforms, Protein Structure, Tertiary, Recombinant Proteins, Ubiquitin-Protein Ligases, Erinaceidae,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, CNR, 80134 Napoli, Italy
Department of Biological Sciences, Federico II University, 80134 Napoli, Italy
Department of Molecular Medicine, La Sapienza University, 00161 Roma, Italy
Department of Experimental Medicine la Sapienza University, 00161 Roma, Italy
References:
Li, W., Bengtson, M.H., Ulbrich, A., Matsuda, A., Reddy, V.A., Orth, A., Chanda, S.K., Joazeiro, C.A., Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle's dynamics and signaling (2008) PLoS One, 3, pp. e148
Bardwell, V.J., Treisman, R., The POZ domain: A conserved protein-protein interaction motif (1994) Genes and Development, 8 (14), pp. 1664-1677
Stogios, P.J., Prive, G.G., The BACK domain in BTB-kelch proteins (2004) Trends in Biochemical Sciences, 29 (12), pp. 634-637. , DOI 10.1016/j.tibs.2004.10.003, PII S0968000404002634
Stogios, P.J., Downs, G.S., Jauhal, J.J., Nandra, S.K., Privé, G.G., Sequence and structural analysis of BTB domain proteins (2005) Genome Biol., 6, pp. R82
Dementieva, I.S., Tereshko, V., McCrossan, Z.A., Solomaha, E., Araki, D., Xu, C., Grigorieff, N., Goldstein, S.A., Pentameric assembly of potassium channel tetramerization domain-containing protein 5 (2009) J. Mol. Biol., 387, pp. 175-191
Bayon, Y., Trinidad, A.G., De La Puerta, M.L., Del Carmen Rodriguez, M., Bogetz, J., Rojas, A., De Pereda, J.M., Alonso, A., KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases (2008) FEBS Journal, 275 (15), pp. 3900-3910. , DOI 10.1111/j.1742-4658.2008.06537.x
Van Bogaert, P., Azizieh, R., Desir, J., Aeby, A., De Meirleir, L., Laes, J.-F., Christiaens, F., Abramowicz, M.J., Mutation of a potassium channel-related gene in progressive myoclonic epilepsy (2007) Annals of Neurology, 61 (6), pp. 579-586. , DOI 10.1002/ana.21121
Elks, C.E., Loos, R.J., Sharp, S.J., Langenberg, C., Ring, S.M., Timpson, N.J., Ness, A.R., Ong, K.K., Genetic markers of adult obesity risk are associated with greater early infancyweight gain and growth (2010) PLoS Med., 7, pp. e1000284
Schwenk, J., Metz, M., Zolles, G., Turecek, R., Fritzius, T., Bildl, W., Tarusawa, E., Bettler, B., Native GABA(B) receptors are heteromultimers with a family of auxiliary subunits (2010) Nature, 465, pp. 231-235
Bartoi, T., Rigbolt, K.T., Du, D., Köhr, G., Blagoev, B., Kornau, H.C., GABAB receptor constituents revealed by tandem affinity purification from transgenic mice (2010) J. Biol. Chem., 285, pp. 20625-20633
Di Marcotullio, L., Ferretti, E., De Smaele, E., Screpanti, I., Gulino, A., Suppressorsof hedgehog signaling: Linking aberrant development of neural progenitors and tumorigenesis (2006) Mol. Neurobiol., 34, pp. 193-204
De Smaele, E., Di Marcotullio, L., Ferretti, E., Screpanti, I., Alesse, E., Gulino, A., Chromosome 17p deletion in human medulloblastoma: A missing checkpoint in the Hedgehog pathway (2004) Cell Cycle, 3 (10), pp. 1263-1266
Argenti, B., Gallo, R., Di Marcotullio, L., Ferretti, E., Napolitano, M., Canterini, S., De Smaele, E., Gulino, A., Hedgehog antagonist RENKCTD11 regulates proliferation and apoptosis of developing granule cell progenitors (2005) Journal of Neuroscience, 25 (36), pp. 8338-8346. , DOI 10.1523/JNEUROSCI.2438-05.2005
Ferretti, E., Smaele, E.D., Marcotullio, L.D., Screpanti, I., Gulino, A., Hedgehog checkpoints in medulloblastoma: The chromosome 17p deletion paradigm (2005) Trends in Molecular Medicine, 11 (12), pp. 537-545. , DOI 10.1016/j.molmed.2005.10.005, PII S147149140500239X
Mancarelli, M.M., Zazzeroni, F., Ciccocioppo, L., Capece, D., Po, A., Murgo, S., Di Camillo, R., Alesse, E., The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors (2010) Mol. Cancer, 9, p. 172
Canettieri, G., Di Marcotullio, L., Greco, A., Coni, S., Antonucci, L., Infante, P., Pietrosanti, L., Gulino, A., Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates hedgehog signalling through Gli acetylation (2010) Nat. Cell Biol., 12, pp. 132-142
Gallo, R., Zazzeroni, F., Alesse, E., Mincione, C., Borello, U., Buanne, P., D'Eugenio, R., Gulino, A., REN: A novel, developmentally regulated gene that promotes neural cell differentiation (2002) Journal of Cell Biology, 158 (4), pp. 731-740. , DOI 10.1083/jcb.200202024
Nanao, M.H., Zhou, W., Pfaffinger, P.J., Choe, S., Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal) (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8670-8675. , DOI 10.1073/pnas.1432840100
Jones, T.A., Zou, J.Y., Cowan, S.W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallogr., 47, pp. 110-119
Zheng, N., Schulman, B.A., Song, L., Miller, J.J., Jeffrey, P.D., Wang, P., Chu, C., Pavletich, N.P., Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex (2002) Nature, 416 (6882), pp. 703-709. , DOI 10.1038/416703a
Angers, S., Li, T., Yi, X., MacCoss, M.J., Moon, R.T., Zheng, N., Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery (2006) Nature, 443 (7111), pp. 590-593. , DOI 10.1038/nature05175, PII NATURE05175
Zheng, N., Wang, P., Jeffrey, P.D., Pavletich, N.P., Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases (2000) Cell, 102, pp. 533-539
Kozak, M., Initiation of translation in prokaryotes and eukaryotes (1999) Gene, 234 (2), pp. 187-208. , DOI 10.1016/S0378-1119(99)00210-3, PII S0378111999002103
Peabody, D.S., Translation initiation at non-AUG triplets in mammalian cells (1989) Journal of Biological Chemistry, 264 (9), pp. 5031-5035
Touriol, C., Bornes, S., Bonnal, S., Audigier, S., Prats, H., Prats, A.-C., Vagner, S., Generation of protein isoform diversity by alternative initiation of translation at non-AUG codons (2003) Biology of the Cell, 95 (3-4), pp. 169-178. , DOI 10.1016/S0248-4900(03)00033-9
Tang, X., Orlicky, S., Lin, Z., Willems, A., Neculai, D., Ceccarelli, D., Mercurio, F., Tyers, M., Suprafacial Orientation of the SCFCdc4 Dimer Accommodates Multiple Geometries for Substrate Ubiquitination (2007) Cell, 129 (6), pp. 1165-1176. , DOI 10.1016/j.cell.2007.04.042, PII S009286740700606X
Bardwell, V. J., Treisman, R., The POZ domain: A conserved protein-protein interaction motif (1994) Genes and Development, 8 (14), pp. 1664-1677
Stogios, P. J., Prive, G. G., The BACK domain in BTB-kelch proteins (2004) Trends in Biochemical Sciences, 29 (12), pp. 634-637. , DOI 10. 1016/j. tibs. 2004. 10. 003, PII S0968000404002634
Stogios, P. J., Downs, G. S., Jauhal, J. J., Nandra, S. K., Priv, G. G., Sequence and structural analysis of BTB domain proteins (2005) Genome Biol., 6, pp. R82
Dementieva, I. S., Tereshko, V., McCrossan, Z. A., Solomaha, E., Araki, D., Xu, C., Grigorieff, N., Goldstein, S. A., Pentameric assembly of potassium channel tetramerization domain-containing protein 5 (2009) J. Mol. Biol., 387, pp. 175-191
Elks, C. E., Loos, R. J., Sharp, S. J., Langenberg, C., Ring, S. M., Timpson, N. J., Ness, A. R., Ong, K. K., Genetic markers of adult obesity risk are associated with greater early infancyweight gain and growth (2010) PLoS Med., 7, pp. e1000284
Mancarelli, M. M., Zazzeroni, F., Ciccocioppo, L., Capece, D., Po, A., Murgo, S., Di Camillo, R., Alesse, E., The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors (2010) Mol. Cancer, 9, p. 172
Nanao, M. H., Zhou, W., Pfaffinger, P. J., Choe, S., Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal) (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8670-8675. , DOI 10. 1073/pnas. 1432840100
Jones, T. A., Zou, J. Y., Cowan, S. W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallogr., 47, pp. 110-119
Peabody, D. S., Translation initiation at non-AUG triplets in mammalian cells (1989) Journal of Biological Chemistry, 264 (9), pp. 5031-5035
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Apr; 93(4): 715-724.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 3.022, 5-year impact factor: 3.424
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-79952438310&partnerID=40&md5=ae8de93cd49681613dc1846115c0b865
Keywords: Hedgehog Pathway, Light Scattering, Medulloblastoma, Protein-Protein Interactions, Structure-Function Relationships, Ubiquitination, Adaptor Protein, Cullin3, Kctd11, Ligase, Potassium Channel Tetramerization Domain 11, Unclassified Drug, Article, Biological Activity, Controlled Study, Escherichia Coli, Human, Human Cell, Nonhuman, Protein Binding, Protein Domain, Protein Expression, Protein Variant, Amino Acid Sequence, Base Sequence, Cullin Proteins, Hek293 Cells, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Isoforms, Protein Structure, Tertiary, Recombinant Proteins, Ubiquitin-Protein Ligases, Erinaceidae,
Affiliations:
*** IBB - CNR ***
Institute of Biostructures and Bioimaging, CNR, 80134 Napoli, Italy
Department of Biological Sciences, Federico II University, 80134 Napoli, Italy
Department of Molecular Medicine, La Sapienza University, 00161 Roma, Italy
Department of Experimental Medicine la Sapienza University, 00161 Roma, Italy
References:
Li, W., Bengtson, M.H., Ulbrich, A., Matsuda, A., Reddy, V.A., Orth, A., Chanda, S.K., Joazeiro, C.A., Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle's dynamics and signaling (2008) PLoS One, 3, pp. e148
Bardwell, V.J., Treisman, R., The POZ domain: A conserved protein-protein interaction motif (1994) Genes and Development, 8 (14), pp. 1664-1677
Stogios, P.J., Prive, G.G., The BACK domain in BTB-kelch proteins (2004) Trends in Biochemical Sciences, 29 (12), pp. 634-637. , DOI 10.1016/j.tibs.2004.10.003, PII S0968000404002634
Stogios, P.J., Downs, G.S., Jauhal, J.J., Nandra, S.K., Privé, G.G., Sequence and structural analysis of BTB domain proteins (2005) Genome Biol., 6, pp. R82
Dementieva, I.S., Tereshko, V., McCrossan, Z.A., Solomaha, E., Araki, D., Xu, C., Grigorieff, N., Goldstein, S.A., Pentameric assembly of potassium channel tetramerization domain-containing protein 5 (2009) J. Mol. Biol., 387, pp. 175-191
Bayon, Y., Trinidad, A.G., De La Puerta, M.L., Del Carmen Rodriguez, M., Bogetz, J., Rojas, A., De Pereda, J.M., Alonso, A., KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases (2008) FEBS Journal, 275 (15), pp. 3900-3910. , DOI 10.1111/j.1742-4658.2008.06537.x
Van Bogaert, P., Azizieh, R., Desir, J., Aeby, A., De Meirleir, L., Laes, J.-F., Christiaens, F., Abramowicz, M.J., Mutation of a potassium channel-related gene in progressive myoclonic epilepsy (2007) Annals of Neurology, 61 (6), pp. 579-586. , DOI 10.1002/ana.21121
Elks, C.E., Loos, R.J., Sharp, S.J., Langenberg, C., Ring, S.M., Timpson, N.J., Ness, A.R., Ong, K.K., Genetic markers of adult obesity risk are associated with greater early infancyweight gain and growth (2010) PLoS Med., 7, pp. e1000284
Schwenk, J., Metz, M., Zolles, G., Turecek, R., Fritzius, T., Bildl, W., Tarusawa, E., Bettler, B., Native GABA(B) receptors are heteromultimers with a family of auxiliary subunits (2010) Nature, 465, pp. 231-235
Bartoi, T., Rigbolt, K.T., Du, D., Köhr, G., Blagoev, B., Kornau, H.C., GABAB receptor constituents revealed by tandem affinity purification from transgenic mice (2010) J. Biol. Chem., 285, pp. 20625-20633
Di Marcotullio, L., Ferretti, E., De Smaele, E., Screpanti, I., Gulino, A., Suppressorsof hedgehog signaling: Linking aberrant development of neural progenitors and tumorigenesis (2006) Mol. Neurobiol., 34, pp. 193-204
De Smaele, E., Di Marcotullio, L., Ferretti, E., Screpanti, I., Alesse, E., Gulino, A., Chromosome 17p deletion in human medulloblastoma: A missing checkpoint in the Hedgehog pathway (2004) Cell Cycle, 3 (10), pp. 1263-1266
Argenti, B., Gallo, R., Di Marcotullio, L., Ferretti, E., Napolitano, M., Canterini, S., De Smaele, E., Gulino, A., Hedgehog antagonist RENKCTD11 regulates proliferation and apoptosis of developing granule cell progenitors (2005) Journal of Neuroscience, 25 (36), pp. 8338-8346. , DOI 10.1523/JNEUROSCI.2438-05.2005
Ferretti, E., Smaele, E.D., Marcotullio, L.D., Screpanti, I., Gulino, A., Hedgehog checkpoints in medulloblastoma: The chromosome 17p deletion paradigm (2005) Trends in Molecular Medicine, 11 (12), pp. 537-545. , DOI 10.1016/j.molmed.2005.10.005, PII S147149140500239X
Mancarelli, M.M., Zazzeroni, F., Ciccocioppo, L., Capece, D., Po, A., Murgo, S., Di Camillo, R., Alesse, E., The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors (2010) Mol. Cancer, 9, p. 172
Canettieri, G., Di Marcotullio, L., Greco, A., Coni, S., Antonucci, L., Infante, P., Pietrosanti, L., Gulino, A., Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates hedgehog signalling through Gli acetylation (2010) Nat. Cell Biol., 12, pp. 132-142
Gallo, R., Zazzeroni, F., Alesse, E., Mincione, C., Borello, U., Buanne, P., D'Eugenio, R., Gulino, A., REN: A novel, developmentally regulated gene that promotes neural cell differentiation (2002) Journal of Cell Biology, 158 (4), pp. 731-740. , DOI 10.1083/jcb.200202024
Nanao, M.H., Zhou, W., Pfaffinger, P.J., Choe, S., Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal) (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8670-8675. , DOI 10.1073/pnas.1432840100
Jones, T.A., Zou, J.Y., Cowan, S.W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallogr., 47, pp. 110-119
Zheng, N., Schulman, B.A., Song, L., Miller, J.J., Jeffrey, P.D., Wang, P., Chu, C., Pavletich, N.P., Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex (2002) Nature, 416 (6882), pp. 703-709. , DOI 10.1038/416703a
Angers, S., Li, T., Yi, X., MacCoss, M.J., Moon, R.T., Zheng, N., Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery (2006) Nature, 443 (7111), pp. 590-593. , DOI 10.1038/nature05175, PII NATURE05175
Zheng, N., Wang, P., Jeffrey, P.D., Pavletich, N.P., Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases (2000) Cell, 102, pp. 533-539
Kozak, M., Initiation of translation in prokaryotes and eukaryotes (1999) Gene, 234 (2), pp. 187-208. , DOI 10.1016/S0378-1119(99)00210-3, PII S0378111999002103
Peabody, D.S., Translation initiation at non-AUG triplets in mammalian cells (1989) Journal of Biological Chemistry, 264 (9), pp. 5031-5035
Touriol, C., Bornes, S., Bonnal, S., Audigier, S., Prats, H., Prats, A.-C., Vagner, S., Generation of protein isoform diversity by alternative initiation of translation at non-AUG codons (2003) Biology of the Cell, 95 (3-4), pp. 169-178. , DOI 10.1016/S0248-4900(03)00033-9
Tang, X., Orlicky, S., Lin, Z., Willems, A., Neculai, D., Ceccarelli, D., Mercurio, F., Tyers, M., Suprafacial Orientation of the SCFCdc4 Dimer Accommodates Multiple Geometries for Substrate Ubiquitination (2007) Cell, 129 (6), pp. 1165-1176. , DOI 10.1016/j.cell.2007.04.042, PII S009286740700606X
Bardwell, V. J., Treisman, R., The POZ domain: A conserved protein-protein interaction motif (1994) Genes and Development, 8 (14), pp. 1664-1677
Stogios, P. J., Prive, G. G., The BACK domain in BTB-kelch proteins (2004) Trends in Biochemical Sciences, 29 (12), pp. 634-637. , DOI 10. 1016/j. tibs. 2004. 10. 003, PII S0968000404002634
Stogios, P. J., Downs, G. S., Jauhal, J. J., Nandra, S. K., Priv, G. G., Sequence and structural analysis of BTB domain proteins (2005) Genome Biol., 6, pp. R82
Dementieva, I. S., Tereshko, V., McCrossan, Z. A., Solomaha, E., Araki, D., Xu, C., Grigorieff, N., Goldstein, S. A., Pentameric assembly of potassium channel tetramerization domain-containing protein 5 (2009) J. Mol. Biol., 387, pp. 175-191
Elks, C. E., Loos, R. J., Sharp, S. J., Langenberg, C., Ring, S. M., Timpson, N. J., Ness, A. R., Ong, K. K., Genetic markers of adult obesity risk are associated with greater early infancyweight gain and growth (2010) PLoS Med., 7, pp. e1000284
Mancarelli, M. M., Zazzeroni, F., Ciccocioppo, L., Capece, D., Po, A., Murgo, S., Di Camillo, R., Alesse, E., The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors (2010) Mol. Cancer, 9, p. 172
Nanao, M. H., Zhou, W., Pfaffinger, P. J., Choe, S., Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family values (FamVal) (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8670-8675. , DOI 10. 1073/pnas. 1432840100
Jones, T. A., Zou, J. Y., Cowan, S. W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallogr., 47, pp. 110-119
Peabody, D. S., Translation initiation at non-AUG triplets in mammalian cells (1989) Journal of Biological Chemistry, 264 (9), pp. 5031-5035
Molecular organization of the cullin E3 ligase adaptor KCTD11
The family of human proteins containing a potassium channel tetramerization domain (KCTD) includes 21 members whose function is largely unknown. Recent reports have however suggested that these proteins are implicated in very important biological processes. KCTD11/REN, the best-characterized member of the family to date, plays a crucial role in the ubiquitination of HDAC1 by acting, in complex with Cullin3, as an E3 ubiquitin ligase. By combining bioinformatics and mutagenesis analyses, here we show that the protein is expressed in two alternative variants: a short previously characterized form (sKCTD11) composed by 232 amino acids and a longer variant (lKCTD11) which contains an N-terminal extension of 39 residues. Interestingly, we demonstrate that lKCTD11 starts with a non-canonical AUU codon. Although both sKCTD11 and lKCTD11 bear a POZ/BTB domain in their N-terminal region, this domain is complete only in the long form. Indeed, sKCTD11 presents an incomplete POZ/BTB domain. Nonetheless, sKCTD11 is still able to bind Cul3, although to much lesser extent than lKCTD11, and to perform its biological activity. The heterologous expression of sKCTD11 and lKCTD11 and their individual domains in Escherichia coli yielded soluble products as fusion proteins only for the longer form. In contrast to the closely related KCTD5 which is pentameric, the characterization of both lKCTD11 and its POZ/BTB domain by gel filtration and light scattering indicates that the protein likely forms stable tetramers. In line with this result, experiments conducted in cells show that the active protein is not monomeric. Based on these findings, homology-based models were built for lKCTD11 BTB and for its complex with Cul3. These analyses indicate that a stable lKCTD11 BTB-Cul3 three-dimensional model with a 4:4 stoichiometry can be generated. Moreover, these models provide insights into the determinants of the tetramer stability and into the regions involved in lKCTD11-Cul3 recognition. © 2011 Elsevier Masson SAS. All rights reserved.
The family of human proteins containing a potassium channel tetramerization domain (KCTD) includes 21 members whose function is largely unknown. Recent reports have however suggested that these proteins are implicated in very important biological processes. KCTD11/REN, the best-characterized member of the family to date, plays a crucial role in the ubiquitination of HDAC1 by acting, in complex with Cullin3, as an E3 ubiquitin ligase. By combining bioinformatics and mutagenesis analyses, here we show that the protein is expressed in two alternative variants: a short previously characterized form (sKCTD11) composed by 232 amino acids and a longer variant (lKCTD11) which contains an N-terminal extension of 39 residues. Interestingly, we demonstrate that lKCTD11 starts with a non-canonical AUU codon. Although both sKCTD11 and lKCTD11 bear a POZ/BTB domain in their N-terminal region, this domain is complete only in the long form. Indeed, sKCTD11 presents an incomplete POZ/BTB domain. Nonetheless, sKCTD11 is still able to bind Cul3, although to much lesser extent than lKCTD11, and to perform its biological activity. The heterologous expression of sKCTD11 and lKCTD11 and their individual domains in Escherichia coli yielded soluble products as fusion proteins only for the longer form. In contrast to the closely related KCTD5 which is pentameric, the characterization of both lKCTD11 and its POZ/BTB domain by gel filtration and light scattering indicates that the protein likely forms stable tetramers. In line with this result, experiments conducted in cells show that the active protein is not monomeric. Based on these findings, homology-based models were built for lKCTD11 BTB and for its complex with Cul3. These analyses indicate that a stable lKCTD11 BTB-Cul3 three-dimensional model with a 4:4 stoichiometry can be generated. Moreover, these models provide insights into the determinants of the tetramer stability and into the regions involved in lKCTD11-Cul3 recognition. © 2011 Elsevier Masson SAS. All rights reserved.
Molecular organization of the cullin E3 ligase adaptor KCTD11
Molecular organization of the cullin E3 ligase adaptor KCTD11
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Riccardi C, Musumeci D, Capuozzo A, Irace C, King S, Russo Krauss I, Paduano L, Montesarchio D
* "dressing up" an Old Drug: An Aminoacyl Lipid for the Functionalization of Ru(III)-Based Anticancer Agents (197 views)
Acs Biomater Sci Eng (ISSN: 2373-9878), 2018; 4(1): 163-174.
Impact Factor: 0
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Gaglione R, Smaldone G, Di Girolamo R, Piccoli R, Pedone E, Arciello A
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Impact Factor: 4.28
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Oliveri V, Bellia F, Vecchio G
* Cyclodextrin Nanoparticles Bearing 8-Hydroxyquinoline Ligands as Multifunctional Biomaterials (737 views)
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Impact Factor: 5.16
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Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
* Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions (279 views) (PDF 172 views)
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Impact Factor: 5.317
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Pirone L, Smaldone G, Esposito C, Balasco N, Petoukhov MV, Spilotros A, Svergun DI, Di Gaetano S, Vitagliano L, Pedone E
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Impact Factor: 3.112
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Barone D, Balasco N, Vitagliano L
* KCTD5 is endowed with large, functionally relevant, interdomain motions (235 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2016 Aug; 34(8): 1725-1735.
Impact Factor: 3.123
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Nicastro MC, Spigolon D, Librizzi F, Moran O, Ortore MG, Bulone D, Biagio PLS, Carrotta R
* Amyloid β-peptide insertion in liposomes containing GM1-cholesterol domains (363 views)
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Impact Factor: 2.402
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Bottini A, Wu B, Barile E, De SK, Leone M, Pellecchia M
* High-Throughput Screening (HTS) by NMR Guided Identification of Novel Agents Targeting the Protein Docking Domain of YopH (422 views)
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Impact Factor: 2.98
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Calce E, Ringhieri P, Mercurio FA, Leone M, Bugatti V, Saviano M, Vittoria V, De Luca S
* A biocompatible process to prepare hyaluronan-based material able to self-assemble into stable nano-particles (377 views)
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Impact Factor: 3.289
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Vitiello G, Falanga A, Petruk AA, Merlino A, Fragneto G, Paduano L, Galdiero S, D'Errico G
* Fusion of raft-like lipid bilayers operated by a membranotropic domain of the HSV-type I glycoprotein gH occurs through a cholesterol-dependent mechanism (381 views)
Soft Matter (ISSN: 1744-683x), 2015; 11(15): 3003-3016.
Impact Factor: 3.798
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Correale S, de Paola I, Morgillo CM, Federico A, Zaccaro L, Pallante P, Galeone A, Fusco A, Pedone E, Luque FJ, Catalanotti B
* Structural Model of the hUbA1-UbcH10 Quaternary Complex: In Silico and Experimental Analysis of the Protein-Protein Interactions between E1, E2 and Ubiquitin (434 views)
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Impact Factor: 3.234
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Saghyan AS, Simonyan HM, Petrosyan SG, Geolchanyan AV, Roviello GN, Musumeci D, Roviello V
* Thiophenyl-substituted triazolyl-thione L-alanine: asymmetric synthesis, aggregation and biological properties (489 views)
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Impact Factor: 3.293
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Balasco N, Pirone L, Smaldone G, Di Gaetano S, Esposito L, Pedone E, Vitagliano L
* Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations (366 views)
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Impact Factor: 4.882
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Tomasello MF, Sinopoli A, Attanasio F, Giuffrida ML, Campagna T, Milardi D, Pappalardo G
* Molecular and cytotoxic properties of hIAPP17-29 and rIAPP17-29 fragments: A comparative study with the respective full-length parent polypeptides (1043 views)
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Impact Factor: 3.447
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Accardo A, Ringhieri P, Szekely N, Pipich V, Luchini A, Paduano L, Tesauro D
* Structural insights on nanoparticles containing gadolinium complexes as potential theranostic (454 views)
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Impact Factor: 1.865
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Cefalo G, Massimino M, Ruggiero A, Barone G, Ridola V, Spreafico F, Potepan P, Abate ME, Mascarin M, Garrè ML, Perilongo G, Madon E, Colosimo C, Riccardi R
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Impact Factor: 6.776
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Villari V, Attanasio F, Micali N
* Control of the structural stability of α-crystallin under thermal and chemical stress: The role of carnosine (411 views)
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Impact Factor: 3.302
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Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E
* Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family (383 views)
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Impact Factor: 9.112
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Roviello GN, Roviello GN, Musumeci D, Capasso D, Di Gaetano S, Costanzo M, Pedone C
* Synthesis and supramolecular assembly of 1, 3-bis(1 '-uracilyl)-2-propanone (404 views)
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Impact Factor: 3.84
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Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone E, Fattorusso R
* Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates (389 views)
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Impact Factor: 5.696
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Accardo A, Mangiapia G, Paduano L, Morelli G, Tesauro D
* Octreotide labeled aggregates containing platinum complexes as nanovectors for drug delivery (407 views)
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Impact Factor: 1.862
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Attanasio F, Convertino M, Magno A, Caflisch A, Corazza A, Haridas H, Esposito G, Cataldo S, Pignataro B, Milardi D, Rizzarelli E
* Carnosine Inhibits Abeta(42) Aggregation By Perturbing The H-Bond Network In And Around The Central Hydrophobic Cluster (697 views)
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Impact Factor: 3.06
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (414 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (342 views)
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Impact Factor: 2.92
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Accardo A, Ringhieri P, Tesauro D, Morelli G
* Liposomes derivatized with tetrabranched neurotensin peptides via click chemistry reactions (397 views)
New J Chem (ISSN: 1144-0546), 2013; 37(11): 3528-3534.
Impact Factor: 3.159
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Roviello GN, Mottola A, Musumeci D, Bucci EM, Pedone C
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Impact Factor: 3.914
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Scarí G, Porta F, Fascio U, Avvakumova S, Dal Santo V, De Simone M, Saviano M, Leone M, Del Gatto A, Pedone C, Zaccaro L
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Impact Factor: 4.58
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Tarallo R, Accardo A, Falanga A, Guarnieri D, Vitiello G, Netti P, D'Errico G, Morelli G, Galdiero S
* Clickable functionalization of liposomes with the gH625 peptide from herpes simplex virus type i for intracellular drug delivery (457 views)
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Impact Factor: 5.925
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Ruvo M, Sandomenico A, Tudisco L, De Falco S
* Branched peptides for the modulation of protein-protein interactions: More arms are better than one? (523 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2011 Jun; 18(16): 2429-2437.
Impact Factor: 4.859
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Pirone L, Correale S, De Paola I, Zaccaro L, De Simone G, Vitagliano L, Pedone E, Di Gaetano S
* Design, synthesis and characterization of a peptide able to bind proteins of the KCTD family: implications for KCTD - cullin 3 recognition (416 views)
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Impact Factor: 1.799
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Riccio A, Mangiapia G, Giordano D, Flagiello A, Tedesco R, Bruno S, Vergara A, Mazzarella L, di Prisco G, Pucci P, Paduano L, Verde C
* Polymerization of Hemoglobins in Arctic Fish: Lycodes reticulatus and Gadus morhua (361 views)
Iubmb Life (ISSN: 1521-6543, 1521-6551), 2011 May; 63(5): 346-354.
Impact Factor: 3.514
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Falciani C, Accardo A, Brunetti J, Tesauro D, Lelli B, Pini A, Bracci L, Morelli G
* Target-Selective Drug Delivery through Liposomes Labeled with Oligobranched Neurotensin Peptides (561 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7187electronic), 2011 Apr 4; 6(4): 678-685.
Impact Factor: 3.151
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Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (674 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
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Canettieri G, Di Marcotullio L, Greco A, Coni S, Antonucci L, Infante P, Pietrosanti L, De Smaele E, Ferretti E, Miele E, Pelloni M, De Simone G, Pedone E, Gallinari P, Giorgi A, Steinkuhler C, Vitagliano L, Pedone C, Schinin ME, Screpanti I, Gulino A
* Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation (611 views)
Nature Cell Biol Nature Cell Biology (ISSN: 1476-4679, 1465-7392), 2010 Feb; 12(2): 132-142.
Impact Factor: 19.407
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Severino V, Chambery A, Di Maro A, Marasco D, Ruggiero A, Berisio R, Giansanti F, Ippoliti R, Parente A
* The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves (396 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(3): 570-579.
Impact Factor: 3.825
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Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (558 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
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Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Del Vecchio L, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M
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Impact Factor: 4.351
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Roviello GN, Musumeci D, Moccia M, Castiglione M, Cesarani A, Bucci EM, Saviano M, Pedone C, Benedetti E
* Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone (431 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 147-154.
Impact Factor: 1.807
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Molecular dynamics studies of the P pilus rod subunit PapA (485 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 192-199.
Impact Factor: 1.807
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Randazzo R, Lauceri R, Mammana A, D'Urso A, Purrello R
* Interactions of Λ and Δ enantiomers of ruthenium(II) cationic complexes with achiral anionic porphyrins (361 views)
Chirality (ISSN: 0899-0042), 2009; 21(1): 92-96.
Impact Factor: 2.677
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Merlino A, Varriale S, Coscia MR, Mazzarella L, Oreste U
* Structure and dimerization of the teleost transmembrane immunoglobulin region (347 views)
Journal Of Molecular Graphics & Modelling (ISSN: 1093-3263), 2008 Oct; 27(3): 401-407.
Impact Factor: 2.347
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Conformational states and association mechanism of Yersinia pestis Caf1 subunits (322 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2008 Sep 8; 372(4): 804-810.
Impact Factor: 2.648
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Ruvo M, Marasco D, Viparelli F, Tornatore L, Sandomenico A, Doti N, Pedone C, Benedetti E, Monti SM
* Peptide antagonists of protein-protein interactions identified by screening protein fragments derived by enzyme degradation (349 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008 Sep; 14(8): N/D-N/D.
Impact Factor: 1.654
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Accardo A, Tesauro D, Aloj L, Tarallo L, Arra C, Mangiapia G, Vaccaro M, Pedone C, Paduano L, Morelli G
* Peptide-containing aggregates as selective nanocarriers for therapeutics (391 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7187electronic), 2008 Apr; 3(4): 594-602.
Impact Factor: 3.15
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Vaccaro M, Accardo A, D'Errico G, Schillen K, Radulescu A, Tesauro D, Morelli G, Paduano L
* Peptides and gd complexes containing colloidal assemblies as tumor-specific contrast agents in MRI: Physicochemical characterization (399 views)
Biophysical Journal (ISSN: 0006-3495, 1542-0086), 2007 Sep; 93(5): 1736-1746.
Impact Factor: 4.627
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Accardo A, Tesauro D, Morelli G, Gianolio E, Aime S, Vaccaro M, Mangiapia G, Paduano L, Schillén K
* High-relaxivity supramolecular aggregates containing peptides and Gd complexes as contrast agents in MRI (423 views)
J Biol Inorg Chem (ISSN: 0949-8257, 1432-1327electronic, 0949-8257print), 2007 Feb; 12(2): 267-276.
Impact Factor: 3.325
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Autiero M, Liuzzi R, Riccio P, Roberti G
* Determination of the concentration scaling law of the scattering coefficient of water solutions of intralipid at 832 nm by comparison between collimated detection measurements and Monte Carlo simulations (311 views)
Lasers Surg Med (ISSN: 0196-8092, 0196-8092print, 0196-8092linking), 2005 Jun; 36(5): 414-422.
Impact Factor: 2.249
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Miralbell R, Lomax A, Cella L, Schneider U
* Potential reduction of the incidence of radiation-induced second cancers by using proton beams in the treatment of pediatric tumors (262 views)
Int J Radiat Oncol (ISSN: 0360-3016, 1879-355x, 1879-355xelectronic), 2002 Nov 1; 54(3): 824-829.
Impact Factor: 3.756
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* Protein Interaction Domains: structural features and drug discovery applications (part 2) (78 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2021; 28(5): 854-892.
Impact Factor: 4.184
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Tornatore L, Capece D, Sandomenico A, Verzella D, Vecchiotti D, Zazzeroni F, Ruvo M, Franzoso G
* The Screening of Combinatorial Peptide Libraries for Targeting Key Molecules or Protein-Protein Interactions in the NF-κB Pathway (8 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2366: 343-356.
Impact Factor: 1.17
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Mercurio FA, Di Natale C, Pirone L, Vincenzi M, Marasco D, De Luca S, Pedone EM, Leone M
* Exploring the ability of cyclic peptides to target SAM domains: a computational and experimental study (266 views)
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Impact Factor: 2.641
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Vincenzi M, Mercurio FA, Leone M
* Sam Domains in Multiple Diseases (237 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2020; 27(3): 450-476.
Impact Factor: 3.469
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Ferrucci V, De Antonellis P, Pennino FP, Asadzadeh F, Virgilio A, Montanaro D, Galeone A, Boffa I, Pisano I, Scognamiglio I, Navas L, Diana D, Pedone E, Gargiulo S, Gramanzini M, Brunetti A, Danielson L, Carotenuto M, Liguori L, Verrico A, Quaglietta L, Errico ME, Del Monaco V, D'Argenio V, Tirone F, Mastronuzzi A, Donofrio V, Giangaspero F, Picard D, Remke M, Garzia L, Daniels C, Delattre O, Swartling FJ, Weiss WA, Salvatore F, Fattorusso R, Chesler L, Taylor MD, Cinalli G, Zollo M
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Impact Factor: 9.915
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Riccardi C, Musumeci D, Capuozzo A, Irace C, King S, Russo Krauss I, Paduano L, Montesarchio D
* "dressing up" an Old Drug: An Aminoacyl Lipid for the Functionalization of Ru(III)-Based Anticancer Agents (197 views)
Acs Biomater Sci Eng (ISSN: 2373-9878), 2018; 4(1): 163-174.
Impact Factor: 0
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Gaglione R, Smaldone G, Di Girolamo R, Piccoli R, Pedone E, Arciello A
* Cell milieu significantly affects the fate of AApoAI amyloidogenic variants: predestination or serendipity? (387 views)
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Impact Factor: 4.28
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Oliveri V, Bellia F, Vecchio G
* Cyclodextrin Nanoparticles Bearing 8-Hydroxyquinoline Ligands as Multifunctional Biomaterials (737 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2017; 23(18): 4442-4449.
Impact Factor: 5.16
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Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
* Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions (279 views) (PDF 172 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2016 Dec 05; 22(49): 17767-17775.
Impact Factor: 5.317
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Pirone L, Smaldone G, Esposito C, Balasco N, Petoukhov MV, Spilotros A, Svergun DI, Di Gaetano S, Vitagliano L, Pedone E
* Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners (323 views) (PDF 192 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016 Sep 24; 131: 106-114.
Impact Factor: 3.112
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Barone D, Balasco N, Vitagliano L
* KCTD5 is endowed with large, functionally relevant, interdomain motions (235 views)
J Biomol Struct Dyn (ISSN: 0739-1102, 1538-0254electronic, 0739-1102linking), 2016 Aug; 34(8): 1725-1735.
Impact Factor: 3.123
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Nicastro MC, Spigolon D, Librizzi F, Moran O, Ortore MG, Bulone D, Biagio PLS, Carrotta R
* Amyloid β-peptide insertion in liposomes containing GM1-cholesterol domains (363 views)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2016; 208: 9-16.
Impact Factor: 2.402
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Bottini A, Wu B, Barile E, De SK, Leone M, Pellecchia M
* High-Throughput Screening (HTS) by NMR Guided Identification of Novel Agents Targeting the Protein Docking Domain of YopH (422 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7179linking), 2015 Nov 23; 11(8): 919-927.
Impact Factor: 2.98
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Calce E, Ringhieri P, Mercurio FA, Leone M, Bugatti V, Saviano M, Vittoria V, De Luca S
* A biocompatible process to prepare hyaluronan-based material able to self-assemble into stable nano-particles (377 views)
Rsc Adv (ISSN: 2046-2069), 2015; 5(37): 29573-29576.
Impact Factor: 3.289
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Vitiello G, Falanga A, Petruk AA, Merlino A, Fragneto G, Paduano L, Galdiero S, D'Errico G
* Fusion of raft-like lipid bilayers operated by a membranotropic domain of the HSV-type I glycoprotein gH occurs through a cholesterol-dependent mechanism (381 views)
Soft Matter (ISSN: 1744-683x), 2015; 11(15): 3003-3016.
Impact Factor: 3.798
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Correale S, de Paola I, Morgillo CM, Federico A, Zaccaro L, Pallante P, Galeone A, Fusco A, Pedone E, Luque FJ, Catalanotti B
* Structural Model of the hUbA1-UbcH10 Quaternary Complex: In Silico and Experimental Analysis of the Protein-Protein Interactions between E1, E2 and Ubiquitin (434 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2014 Nov 6; 9(11): e112082-e112082.
Impact Factor: 3.234
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Saghyan AS, Simonyan HM, Petrosyan SG, Geolchanyan AV, Roviello GN, Musumeci D, Roviello V
* Thiophenyl-substituted triazolyl-thione L-alanine: asymmetric synthesis, aggregation and biological properties (489 views)
Amino Acids (ISSN: 0939-4451, 1438-2199, 1438-2199electronic), 2014 Oct; 46(10): 2325-2332.
Impact Factor: 3.293
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Balasco N, Pirone L, Smaldone G, Di Gaetano S, Esposito L, Pedone E, Vitagliano L
* Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations (366 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014 Jul; 1844(7): 1289-1298.
Impact Factor: 4.882
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Tomasello MF, Sinopoli A, Attanasio F, Giuffrida ML, Campagna T, Milardi D, Pappalardo G
* Molecular and cytotoxic properties of hIAPP17-29 and rIAPP17-29 fragments: A comparative study with the respective full-length parent polypeptides (1043 views)
Eur J Med Chem (ISSN: 0223-5234, 1768-3254, 0223-5234linking), 2014 Jun 23; 81: 442-455.
Impact Factor: 3.447
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Accardo A, Ringhieri P, Szekely N, Pipich V, Luchini A, Paduano L, Tesauro D
* Structural insights on nanoparticles containing gadolinium complexes as potential theranostic (454 views)
Colloid Polym Sci (ISSN: 0303-402x), 2014 May; 292(5): 1121-1127.
Impact Factor: 1.865
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Cefalo G, Massimino M, Ruggiero A, Barone G, Ridola V, Spreafico F, Potepan P, Abate ME, Mascarin M, Garrè ML, Perilongo G, Madon E, Colosimo C, Riccardi R
* Temozolomide is an active agent in children with recurrent medulloblastoma/primitive neuroectodermal tumor: An Italian multi-institutional phase II trial (365 views)
Neuro-Oncology (ISSN: 1522-8517), 2014 May; 16(5): 748-753.
Impact Factor: 6.776
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Villari V, Attanasio F, Micali N
* Control of the structural stability of α-crystallin under thermal and chemical stress: The role of carnosine (411 views)
J Phys Chem B (ISSN: 1520-6106, 1520-5207, 1520-5207linking), 2014; 118(47): 13770-13776.
Impact Factor: 3.302
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Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E
* Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family (383 views)
Nucleic Acids Res (ISSN: 0305-1048, 1362-4962), 2014; 42(9): 5993-6011.
Impact Factor: 9.112
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Roviello GN, Roviello GN, Musumeci D, Capasso D, Di Gaetano S, Costanzo M, Pedone C
* Synthesis and supramolecular assembly of 1, 3-bis(1 '-uracilyl)-2-propanone (404 views)
Rsc Adv (ISSN: 2046-2069), 2014; 4(54): 28691-28698.
Impact Factor: 3.84
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Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone E, Fattorusso R
* Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates (389 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2013 Sep 9; 19(37): 12217-12220.
Impact Factor: 5.696
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Accardo A, Mangiapia G, Paduano L, Morelli G, Tesauro D
* Octreotide labeled aggregates containing platinum complexes as nanovectors for drug delivery (407 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2013 Apr; 19(4): 190-197.
Impact Factor: 1.862
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Attanasio F, Convertino M, Magno A, Caflisch A, Corazza A, Haridas H, Esposito G, Cataldo S, Pignataro B, Milardi D, Rizzarelli E
* Carnosine Inhibits Abeta(42) Aggregation By Perturbing The H-Bond Network In And Around The Central Hydrophobic Cluster (697 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2013 Mar 18; 14(5): 583-592.
Impact Factor: 3.06
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (414 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (342 views)
Chembiochem (ISSN: 1439-4227, 1439-9422, 1439-7633), 2013 Jan 2; 14(1): 100-106.
Impact Factor: 2.92
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Accardo A, Ringhieri P, Tesauro D, Morelli G
* Liposomes derivatized with tetrabranched neurotensin peptides via click chemistry reactions (397 views)
New J Chem (ISSN: 1144-0546), 2013; 37(11): 3528-3534.
Impact Factor: 3.159
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Roviello GN, Mottola A, Musumeci D, Bucci EM, Pedone C
* Synthesis and aggregation properties of a novel enzymatically resistant nucleoamino acid (423 views)
Amino Acids (ISSN: 0939-4451, 1438-2199, 1438-2199electronic), 2012 Oct; 43(4): 1465-1470.
Impact Factor: 3.914
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Scarí G, Porta F, Fascio U, Avvakumova S, Dal Santo V, De Simone M, Saviano M, Leone M, Del Gatto A, Pedone C, Zaccaro L
* Gold nanoparticles capped by a GC-containing peptide functionalized with an RGD motif for integrin targeting (561 views)
Bioconjug Chem (ISSN: 1520-4812, 1043-1802, 1520-4812electronic), 2012 Mar 21; 23(3): 340-349.
Impact Factor: 4.58
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Tarallo R, Accardo A, Falanga A, Guarnieri D, Vitiello G, Netti P, D'Errico G, Morelli G, Galdiero S
* Clickable functionalization of liposomes with the gH625 peptide from herpes simplex virus type i for intracellular drug delivery (457 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2011 Nov 4; 17(45): 12659-12668.
Impact Factor: 5.925
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Ruvo M, Sandomenico A, Tudisco L, De Falco S
* Branched peptides for the modulation of protein-protein interactions: More arms are better than one? (523 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2011 Jun; 18(16): 2429-2437.
Impact Factor: 4.859
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Pirone L, Correale S, De Paola I, Zaccaro L, De Simone G, Vitagliano L, Pedone E, Di Gaetano S
* Design, synthesis and characterization of a peptide able to bind proteins of the KCTD family: implications for KCTD - cullin 3 recognition (416 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2011 May; 17(5): 373-376.
Impact Factor: 1.799
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Riccio A, Mangiapia G, Giordano D, Flagiello A, Tedesco R, Bruno S, Vergara A, Mazzarella L, di Prisco G, Pucci P, Paduano L, Verde C
* Polymerization of Hemoglobins in Arctic Fish: Lycodes reticulatus and Gadus morhua (361 views)
Iubmb Life (ISSN: 1521-6543, 1521-6551), 2011 May; 63(5): 346-354.
Impact Factor: 3.514
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Falciani C, Accardo A, Brunetti J, Tesauro D, Lelli B, Pini A, Bracci L, Morelli G
* Target-Selective Drug Delivery through Liposomes Labeled with Oligobranched Neurotensin Peptides (561 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7187electronic), 2011 Apr 4; 6(4): 678-685.
Impact Factor: 3.151
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Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (674 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
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Canettieri G, Di Marcotullio L, Greco A, Coni S, Antonucci L, Infante P, Pietrosanti L, De Smaele E, Ferretti E, Miele E, Pelloni M, De Simone G, Pedone E, Gallinari P, Giorgi A, Steinkuhler C, Vitagliano L, Pedone C, Schinin ME, Screpanti I, Gulino A
* Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation (611 views)
Nature Cell Biol Nature Cell Biology (ISSN: 1476-4679, 1465-7392), 2010 Feb; 12(2): 132-142.
Impact Factor: 19.407
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Severino V, Chambery A, Di Maro A, Marasco D, Ruggiero A, Berisio R, Giansanti F, Ippoliti R, Parente A
* The role of the glycan moiety on the structure-function relationships of PD-L1, type 1 ribosome-inactivating protein from P. dioica leaves (396 views)
Mol Biosyst (ISSN: 1742-206x), 2010; 6(3): 570-579.
Impact Factor: 3.825
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Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (558 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
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Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Del Vecchio L, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M
* MicroRNA-199b-5p Impairs Cancer Stem Cells through Negative Regulation of HES1 in Medulloblastoma (442 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2009 Mar 24; 4(3): e4998-e4998.
Impact Factor: 4.351
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Roviello GN, Musumeci D, Moccia M, Castiglione M, Cesarani A, Bucci EM, Saviano M, Pedone C, Benedetti E
* Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone (431 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 147-154.
Impact Factor: 1.807
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Molecular dynamics studies of the P pilus rod subunit PapA (485 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 192-199.
Impact Factor: 1.807
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Randazzo R, Lauceri R, Mammana A, D'Urso A, Purrello R
* Interactions of Λ and Δ enantiomers of ruthenium(II) cationic complexes with achiral anionic porphyrins (361 views)
Chirality (ISSN: 0899-0042), 2009; 21(1): 92-96.
Impact Factor: 2.677
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Merlino A, Varriale S, Coscia MR, Mazzarella L, Oreste U
* Structure and dimerization of the teleost transmembrane immunoglobulin region (347 views)
Journal Of Molecular Graphics & Modelling (ISSN: 1093-3263), 2008 Oct; 27(3): 401-407.
Impact Factor: 2.347
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Conformational states and association mechanism of Yersinia pestis Caf1 subunits (322 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2008 Sep 8; 372(4): 804-810.
Impact Factor: 2.648
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Ruvo M, Marasco D, Viparelli F, Tornatore L, Sandomenico A, Doti N, Pedone C, Benedetti E, Monti SM
* Peptide antagonists of protein-protein interactions identified by screening protein fragments derived by enzyme degradation (349 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008 Sep; 14(8): N/D-N/D.
Impact Factor: 1.654
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Accardo A, Tesauro D, Aloj L, Tarallo L, Arra C, Mangiapia G, Vaccaro M, Pedone C, Paduano L, Morelli G
* Peptide-containing aggregates as selective nanocarriers for therapeutics (391 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7187electronic), 2008 Apr; 3(4): 594-602.
Impact Factor: 3.15
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Vaccaro M, Accardo A, D'Errico G, Schillen K, Radulescu A, Tesauro D, Morelli G, Paduano L
* Peptides and gd complexes containing colloidal assemblies as tumor-specific contrast agents in MRI: Physicochemical characterization (399 views)
Biophysical Journal (ISSN: 0006-3495, 1542-0086), 2007 Sep; 93(5): 1736-1746.
Impact Factor: 4.627
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Accardo A, Tesauro D, Morelli G, Gianolio E, Aime S, Vaccaro M, Mangiapia G, Paduano L, Schillén K
* High-relaxivity supramolecular aggregates containing peptides and Gd complexes as contrast agents in MRI (423 views)
J Biol Inorg Chem (ISSN: 0949-8257, 1432-1327electronic, 0949-8257print), 2007 Feb; 12(2): 267-276.
Impact Factor: 3.325
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Autiero M, Liuzzi R, Riccio P, Roberti G
* Determination of the concentration scaling law of the scattering coefficient of water solutions of intralipid at 832 nm by comparison between collimated detection measurements and Monte Carlo simulations (311 views)
Lasers Surg Med (ISSN: 0196-8092, 0196-8092print, 0196-8092linking), 2005 Jun; 36(5): 414-422.
Impact Factor: 2.249
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Miralbell R, Lomax A, Cella L, Schneider U
* Potential reduction of the incidence of radiation-induced second cancers by using proton beams in the treatment of pediatric tumors (262 views)
Int J Radiat Oncol (ISSN: 0360-3016, 1879-355x, 1879-355xelectronic), 2002 Nov 1; 54(3): 824-829.
Impact Factor: 3.756
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53 Records (50 excluding Abstracts).
Total impact factor: 205.84 (196.601 excluding Abstracts).
Total 5 year impact factor: 217.491 (207.624 excluding Abstracts).
Total impact factor: 205.84 (196.601 excluding Abstracts).
Total 5 year impact factor: 217.491 (207.624 excluding Abstracts).
435 views. Last view on Saturday 11 March 2023, 21:45:09
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