BB14, a Nerve Growth Factor (NGF)-like peptide shown to be effective in reducing reactive astrogliosis and restoring synaptic homeostasis in a rat model of peripheral nerve injury (409 views)
Biotechnology Advances (ISSN: 0734-9750), 2012 Jan; 30(1): 223-232.
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Paper type: Journal Article,
Impact factor: 9.599, 5-year impact factor: 11.85
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84855746317&partnerID=40&md5=373569bf397db8f269323fef5c3174f8
Keywords: Nerve Injury, Neuro-Glial Network, Ngf-Like Peptide, Reactive Astrocytosis, Synaptic Homeostasis, Astrogliosis, Beneficial Effects, Gabaergic, Glutamate Transporters, Glutathiones, Hyperalgesia, Immunohistochemical Analysis, Molecular Changes, Nerve Growth Factor, Nerve Injuries, Neurodegenerative Disorders, Neuropathic Pain, Neuroplastic Changes, Neuroprotection, Pathological Conditions, Peptidomimetics, Peripheral Nerves, Rat Model, Sciatic Nerves, Spinal Cords, Therapeutic Agents, Dosimetry, Neurodegenerative Diseases, Physiology, Analgesic Agent, Glutamic Acid, Glycine, Biomarker, Correlation, Inhibitor, Animal, Article, Chemistry, Disease Model, Drug Effect, Immunohistochemistry, Metabolism, Peripheral Nerve Injury, Spinal Cord Injury, Sprague Dawley Rat, Peripheral Nerve Injuries, Sprague-Dawley, Spinal Cord Injuries, Synapses, Rattus,
Affiliations:
*** IBB - CNR ***
Laboratory of Morphology of Neural Networks, Department of Medicina Pubblica Clinica e Preventiva, Second University of Napoli, 80138 Napoli, Italy
Laboratory of Neuroscience R. Levi-Montalcini, Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano, Italy
PRIMM Srl, Milano, Italy
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, Department of Biological Sciences, Univ. of Napoli Federico II and Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, 80138 Napoli, Italy
References:
Not available.
Biotechnology Advances (ISSN: 0734-9750), 2012 Jan; 30(1): 223-232.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 9.599, 5-year impact factor: 11.85
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84855746317&partnerID=40&md5=373569bf397db8f269323fef5c3174f8
Keywords: Nerve Injury, Neuro-Glial Network, Ngf-Like Peptide, Reactive Astrocytosis, Synaptic Homeostasis, Astrogliosis, Beneficial Effects, Gabaergic, Glutamate Transporters, Glutathiones, Hyperalgesia, Immunohistochemical Analysis, Molecular Changes, Nerve Growth Factor, Nerve Injuries, Neurodegenerative Disorders, Neuropathic Pain, Neuroplastic Changes, Neuroprotection, Pathological Conditions, Peptidomimetics, Peripheral Nerves, Rat Model, Sciatic Nerves, Spinal Cords, Therapeutic Agents, Dosimetry, Neurodegenerative Diseases, Physiology, Analgesic Agent, Glutamic Acid, Glycine, Biomarker, Correlation, Inhibitor, Animal, Article, Chemistry, Disease Model, Drug Effect, Immunohistochemistry, Metabolism, Peripheral Nerve Injury, Spinal Cord Injury, Sprague Dawley Rat, Peripheral Nerve Injuries, Sprague-Dawley, Spinal Cord Injuries, Synapses, Rattus,
Affiliations:
*** IBB - CNR ***
Laboratory of Morphology of Neural Networks, Department of Medicina Pubblica Clinica e Preventiva, Second University of Napoli, 80138 Napoli, Italy
Laboratory of Neuroscience R. Levi-Montalcini, Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano, Italy
PRIMM Srl, Milano, Italy
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, Department of Biological Sciences, Univ. of Napoli Federico II and Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, 80138 Napoli, Italy
References:
Not available.
BB14, a Nerve Growth Factor (NGF)-like peptide shown to be effective in reducing reactive astrogliosis and restoring synaptic homeostasis in a rat model of peripheral nerve injury
Peptidomimetics hold a great promise as therapeutic agents for neurodegenerative disorders. We previously described a Nerve Growth Factor (NGF)-like peptide, now named BB14, which was found to act as a strong TrkA agonist and to be effective in the sciatic nerve injury model of neuropathic pain. In this report we present the effects of BB14 in reducing reactive astrocytosis and reverting neuroplastic changes of the glutamate/GABAergic circuitry in the lumbar spinal cord following spared nerve injury (SNI) of the sciatic nerve. Immunohistochemical analysis of spinal cord sections revealed that SNI was associated with increased microglial (Iba1) and astrocytic (GFAP) responses, indicative of reactive gliosis. These changes were paralleled by (i) decreased glial aminoacid transporters (GLT1 and GlyT1) and increased levels of (ii) neuronal glutamate transporter EAAC1, (iii) neuronal vesicular GABA transporter (vGAT) and (iv) the GABAergic neuron marker GAD65/67. A remarkable increase of the Glutamate/GABA ratio and the reduction of glutathione (GSH) levels were also indicative of modifications of glial function in neuroprotection. All these molecular changes were found to be linked to an alteration of endogenous NGF metabolism, as demonstrated by decreased levels of mature NGF, increase of proNGF and increased activity of NGF-degrading methallo-proteinases (MMPs). Biochemical alterations and SNI-related neuropathic behavior, characterized by allodynia and hyperalgesia, were reversed by 7-days i.t, administration of the NGF-like peptide BB14, as well as by increasing endogenous NGF levels by it. infusion of GM6001, a MMPs inhibitor. All together, while confirming the correlation between reactive astrogliosis and perturbation of synaptic circuitry in the SNI model of peripheral nerve injury, these data strongly support the beneficial effect of BB14 in reducing reactive astrogliosis and restoring synaptic homeostasis under pathological conditions linked to alteration of NGF availability and signaling, thereby suggesting a potential role of BB14 as a therapeutic agent. (C) 2011 Published by Elsevier Inc.
Peptidomimetics hold a great promise as therapeutic agents for neurodegenerative disorders. We previously described a Nerve Growth Factor (NGF)-like peptide, now named BB14, which was found to act as a strong TrkA agonist and to be effective in the sciatic nerve injury model of neuropathic pain. In this report we present the effects of BB14 in reducing reactive astrocytosis and reverting neuroplastic changes of the glutamate/GABAergic circuitry in the lumbar spinal cord following spared nerve injury (SNI) of the sciatic nerve. Immunohistochemical analysis of spinal cord sections revealed that SNI was associated with increased microglial (Iba1) and astrocytic (GFAP) responses, indicative of reactive gliosis. These changes were paralleled by (i) decreased glial aminoacid transporters (GLT1 and GlyT1) and increased levels of (ii) neuronal glutamate transporter EAAC1, (iii) neuronal vesicular GABA transporter (vGAT) and (iv) the GABAergic neuron marker GAD65/67. A remarkable increase of the Glutamate/GABA ratio and the reduction of glutathione (GSH) levels were also indicative of modifications of glial function in neuroprotection. All these molecular changes were found to be linked to an alteration of endogenous NGF metabolism, as demonstrated by decreased levels of mature NGF, increase of proNGF and increased activity of NGF-degrading methallo-proteinases (MMPs). Biochemical alterations and SNI-related neuropathic behavior, characterized by allodynia and hyperalgesia, were reversed by 7-days i.t, administration of the NGF-like peptide BB14, as well as by increasing endogenous NGF levels by it. infusion of GM6001, a MMPs inhibitor. All together, while confirming the correlation between reactive astrogliosis and perturbation of synaptic circuitry in the SNI model of peripheral nerve injury, these data strongly support the beneficial effect of BB14 in reducing reactive astrogliosis and restoring synaptic homeostasis under pathological conditions linked to alteration of NGF availability and signaling, thereby suggesting a potential role of BB14 as a therapeutic agent. (C) 2011 Published by Elsevier Inc.
BB14, a Nerve Growth Factor (NGF)-like peptide shown to be effective in reducing reactive astrogliosis and restoring synaptic homeostasis in a rat model of peripheral nerve injury
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BB14, a Nerve Growth Factor (NGF)-like peptide shown to be effective in reducing reactive astrogliosis and restoring synaptic homeostasis in a rat model of peripheral nerve injury
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Cirillo G, Bianco MR, Colangelo AM, Cavaliere C, Daniele DL, Zaccaro L, Alberghina L, Papa M
* Reactive astrocytosis-induced perturbation of synaptic homeostasis is restored by nerve growth factor (478 views)
Neurobiology Of Disease (ISSN: 0969-9961), 2011 Mar; 41(3): 630-639.
Impact Factor: 5.403
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* Reactive astrocytosis-induced perturbation of synaptic homeostasis is restored by nerve growth factor (478 views)
Neurobiology Of Disease (ISSN: 0969-9961), 2011 Mar; 41(3): 630-639.
Impact Factor: 5.403
View Export to BibTeX Export to EndNote
1 Records (1 excluding Abstracts).
Total impact factor: 5.403 (5.403 excluding Abstracts).
Total 5 year impact factor: 5.271 (5.271 excluding Abstracts).
Total impact factor: 5.403 (5.403 excluding Abstracts).
Total 5 year impact factor: 5.271 (5.271 excluding Abstracts).
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