Functional and pharmacological characterization of a VEGF mimetic peptide on reparative angiogenesis(607 views) Finetti F, Basile A, Capasso D, Di Gaetano S, Di Stasi R, Pascale M, Turco CM, Ziche M, Morbidelli L, D'Andrea LD
Sezione di Farmacologia, Dipartimento di Biotecnologie, Università degli Studi di Siena, Via A. Moro 2, 53100 Siena, Italy
Dipartimento di Scienze Farmaceutiche e Biomediche, Università di Salerno, Via Ponte Don Melillo, 84084 Fisciano (SA), Italy
Facoltà di Scienze Biotecnologiche, Università di Napoli Federico II, Via Mezzocannone 16, 80134 Napoli, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy
Facolt di Scienze Biotecnologiche, Universit di Napoli Federico II, Via Mezzocannone 16, 80134 Napoli, Italy
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Lee, J. S., Wagoner Johnson, A. J., Murphy, W. L., A modular, hydroxyapatite-binding version of vascular endothelial growth factor (2010) Adv Mater, 22, pp. 5494-5498
Leslie-Barbick, J. E., Saik, J. E., Gould, D. J., Dickinson, M. E., West, J. L., The promotion of microvasculature formation in poly (ethylene glycol) diacrylate hydrogels by an immobilized VEGF-mimetic peptide (2011) Biomaterials, 32, pp. 5782-5789
Webber, M. J., Tongers, J., Newcomb, C. J., Marquardt, K. T., Bauersachs, J., Losordo, D. W., Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair (2011) Proc Natl Acad Sci USA, 108, pp. 13438-13443
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Functional and pharmacological characterization of a VEGF mimetic peptide on reparative angiogenesis
Vascular endothelial growth factor (VEGF) is the main regulator of physiological and pathological angiogenesis. Low molecular weight molecules able to stimulate angiogenesis have interesting medical application for example in regenerative medicine, but at present none has reached the clinic. We reported that a VEGF mimetic helical peptide, QK, designed on the VEGF helix sequence 17-25, is able to bind and activate the VEGF receptors, producing angiogenesis. In this study we evaluate the pharmacological properties of peptide QK with the aim to propose it as a VEGF-mimetic drug to be employed in reparative angiogenesis. We show that the peptide QK is able to recapitulate all the biological activities of VEGF in vivo and on endothelial cells. In experiments evaluating sprouting from aortic ring and vessel formation in an in vivo angiogenesis model, the peptide QK showed biological effects comparable with VEGF. At endothelial level, the peptide up-regulates VEGF receptor expression, activates intracellular pathways depending on VEGFR2, and consistently it induces endothelial cell proliferation, survival and migration. When added to angiogenic factors (VEGF and/or FGF-2), QK produces an improved biological action, which resulted in reduced apoptosis and accelerated in vitro wound healing. The VEGF-like activity of the short peptide QK, characterized by lower cost of production and easier handling compared to the native glycoprotein, suggests that it is an attractive candidate to be further developed for application in therapeutic angiogenesis.(C) 2012 Elsevier Inc. All rights reserved.
Functional and pharmacological characterization of a VEGF mimetic peptide on reparative angiogenesis
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Ntziachristos V, Cuénod CA, Fournier L, Balvay D, Pradel C, Siauve N, Clement O, Jouannot E, Lucidarme O, Vecchio SD, Salvatore M, Law B, Tung C-H, Jain RK, Fukumura D, Munn LL, Brown EB, Schellenberger E, Montet X, Weissleder R, Clerck ND, Postnov A * Tumor Imaging(350 views) Textbook Of In Vivo Imaging In Vertebrates (ISSN: 9780-4700), 2007 Jul 16; 1: 277-309. Impact Factor:1.148 ViewExport to BibTeXExport to EndNote