Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins(332 views) De Rosa L, Cortajarena AL, Romanelli A, Regan L, D'Andrea LD
Keywords: Chemical Biology, Desired Position, Experimental Setup, Expressed Protein Ligation, High Purity, Indispensable Tools, Labeled Proteins, Life-Sciences, Molecular Probes, Peptide Synthesis, Protein Sequences, Single Molecule, Site-Specific, Synthetic Methodology, Synthetic Strategies, Wide Spectrum, Biosynthesis, Article, Chemistry, Fluorescence Resonance Energy Transfer, Protein Engineering, Staining, Staining And Labeling,
Affiliations: *** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, via Mezzocannone 16, 80134, Napoli, Italy
Department of Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT-06511, United States
Dipartimento Delle Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 16, 80134, Napoli, Italy
IMDEA-Nanociencia, Centro Nacional de Biotecnología (CNB-CSIC), Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain
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Kao, M. W. -P., Yang, L. -L., Lin, J. C. -K., Lim, T. -S., Fann, W., Chen, R. P. -Y., Strategy for efficient site-specific FRET-dye labeling of ubiquitin (2008) Bioconjugate Chemistry, 19 (6), pp. 1124-1126. , DOI 10. 1021/bc700480j
Smith, J. J., Conrad, D. W., Cuneo, M. J., Hellinga, H. W., Orthogonal site-specific protein modification by engineering reversible thiol protection mechanisms (2005) Protein Science, 14 (1), pp. 64-73. , DOI 10. 1110/ps. 04965405
Brustad, E. M., Lemke, E. A., Schultz, P. G., Deniz, A. A., A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer (2008) J. Am. Chem. Soc., 130, pp. 17664-17665
Miyake-Stoner, S. J., Miller, A. M., Hammill, J. T., Peeler, J. C., Hess, K. R., Mehl, R. A., Brewer, S. H., Probing protein folding using site-specifically encoded unnatural amino acids as FRET donors with tryptophan (2009) Biochemistry, 48, pp. 5953-5962
Antos, J. M., Chew, G. L., Guimaraes, C. P., Yoder, N. C., Grotenbreg, G. M., Popp, M. W., Ploegh, H. L., Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity (2009) J. Am. Chem. Soc., 131, pp. 10800-10801
Crochet, A. P., Kabir, M. M., Francis, M. B., Paavola, C. D., Site-selective dual modification of periplasmic binding proteins for sensing applications (2010) Biosens. Bioelectron., 26, pp. 55-61
Dawson, P. E., Muir, T. W., Clark-Lewis, I., Kent, S. B. H., Synthesis of proteins by native chemical ligation (1994) Science, 266 (5186), pp. 776-779
Muir, T. W., Sondhi, D., Cole, P. A., Expressed protein ligation: A general method for protein engineering (1998) Proceedings of the National Academy of Sciences of the United States of America, 95 (12), pp. 6705-6710. , DOI 10. 1073/pnas. 95. 12. 6705
Deniz, A. A., Laurence, T. A., Beligere, G. S., Dahan, M., Martin, A. B., Chemla, D. S., Dawson, P. E., Weiss, S., Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2 (2000) Proceedings of the National Academy of Sciences of the United States of America, 97 (10), pp. 5179-5184. , DOI 10. 1073/pnas. 090104997
Cotton, G. J., Muir, T. W., Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation (2000) Chemistry and Biology, 7 (4), pp. 253-261. , DOI 10. 1016/S1074-5521 (00) 00100-9
Yang, J., Yang, W. Y., Site-specific two-color protein labeling for FRET studies using split inteins (2009) J. Am. Chem. Soc., 131, pp. 11644-11645
Main, E. R. G., Xiong, Y., Cocco, M. J., D'Andrea, L., Regan, L., Design of stable -helical arrays from an idealized TPR motif (2003) Structure, 11 (5), pp. 497-508. , DOI 10. 1016/S0969-2126 (03) 00076-5
D'Andrea, L. D., Regan, L., TPR proteins: The versatile helix (2003) Trends in Biochemical Sciences, 28 (12), pp. 655-662. , DOI 10. 1016/j. tibs. 2003. 10. 007
Warrens, A. N., Jones, M. D., Lechler, R. I., Splicing by over-lap extension by PCR using asymmetric amplification: An improved technique for the generation of hybrid proteins of immunological interest (1997) Gene, 186 (1), pp. 29-35. , DOI 10. 1016/S0378-1119 (96) 00674-9, PII S0378111996006749
Southworth, M. W., Amaya, K., Evans, T. C., Xu, M. -Q., Perler, F. B., Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein (1999) BioTechniques, 27 (1), pp. 110-120
Peroza, E. A., Freisinger, E., Tris is a non-innocent buffer during intein-mediated protein cleavage (2008) Protein Expression and Purification, 57 (2), pp. 217-225. , DOI 10. 1016/j. pep. 2007. 10. 003, PII S1046592807002422
Shafer, D. E., Inman, J. K., Lees, A., Reaction of tris (2-carboxyethyl) phosphine (TCEP) with maleimide and - haloacyl groups: Anomalous elution of TCEP by gel filtration (2000) Analytical Biochemistry, 282 (1), pp. 161-164. , DOI 10. 1006/abio. 2000. 4609
Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins