Semi-Synthesis of Labeled Proteins for Spectroscopic Applications (468 views)
Molecules (ISSN: 1420-3049, 1420-3049electronic, 1420-3049linking), 2013 Jan; 18(1): 440-465.
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Paper type: Journal Article,
Impact factor: 2.095, 5-year impact factor: 2.638
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872854831&partnerID=40&md5=ca926f404bd6ff70e014005aa0d6e5b1
Keywords: Expressed Protein Ligation, Förster Resonance Energy Transfer, Intein, Protein Labeling, Segmental Labeling, Fluorescent Dye, Peptide Fragment, Recombinant Protein, Biosynthesis, Chemistry, Fluorescence Resonance Energy Transfer, Genetics, Human, Isotope Labeling, Methodology, Nuclear Magnetic Resonance, Protein Engineering, Protein Motif, Review, Staining, Amino Acid Motifs, Biomolecular, Staining And Labeling, F Rster Resonance Energy Transfer,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy
Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, Via Mezzocannone 16, Napoli 80134, Italy
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Nilsson, B. L., Kiessling, L. L., Raines, R. T., Staudinger ligation: A peptide from a thioester and azide (2000) Org. Lett., 2, pp. 1939-1941
Muir, T. W., Semisynthesis of proteins by expressed protein ligation (2003) Annu. Rev. Biochem., 72, pp. 249-289
Muir, T. W., Sondhi, D., Cole, P. A., Expressed protein ligation: A general method for protein engineering (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 6705-6710
Xu, M. Q., Perler, F. B., The mechanism of protein splicing and its modulation by mutation (1996) EMBO J., 15, pp. 5146-5153
Flavell, R. R., Muir, T. W., Expressed protein ligation (epl) in the study of signal transduction, ion conduction, and chromatin biology (2009) Acc. Chem. Res., 42, pp. 107-116
Southworth, M. W., Amaya, K., Evans, T. C., Xu, M. Q., Perler, F. B., Purification of proteins fused to either the amino or carboxy terminus of the mycobacterium xenopi gyrase a intein (1999) Biotechniques, 27, pp. 110-114. , 116, 118-120
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Aranko, A. S., Zuger, S., Buchinger, E., Iwai, H., In vivo and in vitro protein ligation by naturally occurring and engineered split dnae inteins (2009) PLoS One, 4, pp. e5185
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Heal, W. P., Jovanovic, B., Bessin, S., Wright, M. H., Magee, A. I., Tate, E. W., Bioorthogonal chemical tagging of protein cholesterylation in living cells (2011) Chem. Commun. (Camb), 47, pp. 4081-4083
Cotton, G. J., Muir, T. W., Peptide ligation and its application to protein engineering (1999) Chem. Biol., 6, pp. R247-R256
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Molecules (ISSN: 1420-3049, 1420-3049electronic, 1420-3049linking), 2013 Jan; 18(1): 440-465.
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Paper type: Journal Article,
Impact factor: 2.095, 5-year impact factor: 2.638
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872854831&partnerID=40&md5=ca926f404bd6ff70e014005aa0d6e5b1
Keywords: Expressed Protein Ligation, Förster Resonance Energy Transfer, Intein, Protein Labeling, Segmental Labeling, Fluorescent Dye, Peptide Fragment, Recombinant Protein, Biosynthesis, Chemistry, Fluorescence Resonance Energy Transfer, Genetics, Human, Isotope Labeling, Methodology, Nuclear Magnetic Resonance, Protein Engineering, Protein Motif, Review, Staining, Amino Acid Motifs, Biomolecular, Staining And Labeling, F Rster Resonance Energy Transfer,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy
Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, Via Mezzocannone 16, Napoli 80134, Italy
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Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes. © 2013 by the authors.
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes. © 2013 by the authors.
Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
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Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
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Sciacca MFM, Monaco I, La Rosa C, Milardi D
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Chem Commun (ISSN: 1364-548xelectronic, 1359-7345linking), 2018 Apr 5; 54(29): 3629-3631.
Impact Factor: 6.169
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Avitabile C, Diaferia C, Della Ventura B, Mercurio FA, Leone M, Roviello V, Saviano M, Velotta R, Morelli G, Accardo A, Romanelli A
* Self-Assembling of Fmoc-GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates (891 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Mar 26; 24(18): 4729-4735.
Impact Factor: 5.476
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Deiana M, Mettra B, Martinez-fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K
* Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe (415 views)
J Phys Chem Lett (ISSN: 1948-7185, 1948-7185electronic, 1948-7185linking), 2017 Dec 7; 8(23): 5915-5920.
Impact Factor: 8.709
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Santagata S, Portella L, Napolitano M, Greco A, D'Alterio C, Barone MV, Luciano A, Gramanzini M, Auletta L, Arra C, Zannetti A, Scala S
* A novel CXCR4-targeted near-infrared (NIR) fluorescent probe (Peptide R-NIR750) specifically detects CXCR4 expressing tumors (386 views) (PDF 206 views)
Sci Rep (ISSN: 2045-2322, 2045-2322electronic, 2045-2322linking), 2017 May 31; 7(1): 2554-2554.
Impact Factor: 4.122
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Borchmann DE, Tarallo R, Avendano S, Falanga A, Carberry TP, Galdiero S, Weck M
* Membranotropic peptide-functionalized poly(lactide)- graft -poly(ethylene glycol) brush copolymers for intracellular delivery (434 views)
Macromolecules (ISSN: 0024-9297), 2015; 48(4): 942-949.
Impact Factor: 5.554
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Giuffrida ML, Rizzarelli E, Tomaselli GA, Satriano C, Trusso Sfrazzetto G
* A novel fully water-soluble Cu(i) probe for fluorescence live cell imaging (375 views)
Chem Commun (ISSN: 1359-7345, 1364-548x, 1364-548xelectronic), 2014; 50(69): 9835-9838.
Impact Factor: 6.834
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Doti N, Raimondo D, Sabatella M, Ruvo M
* Identification of Protease Inhibitors by a Fast Fluorimetric Assay (683 views)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2013 Jun; 54(2): 283-291.
Impact Factor: 2.275
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Arena G, Attanasio F, Zhang D, Yang Y, Bartsch RA, Sgarlata C
* Selective sensing of Hg2+ by a proton-ionizable calix[4]arene fluoroionophore (347 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 1133-1137.
Impact Factor: 3.578
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Pica A, Russo Krauss I, Castellano I, Rossi M, La Cara F, Graziano G, Sica F, Merlino A
* Exploring The Unfolding Mechanism Of Gamma-Glutamyltranspeptidases: The Case Of The Thermophilic Enzyme From Geobacillus Thermodenitrificans (566 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2012 Apr; 1824(4): 571-577.
Impact Factor: 3.733
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Musumeci D, Oliviero G, Roviello GN, Bucci EM, Piccialli G
* G-quadruplex-forming oligonucleotide conjugated to magnetic nanoparticles: Synthesis, characterization, and enzymatic stability assays (816 views)
Bioconjug Chem (ISSN: 1043-1802, 1520-4812, 1520-4812electronic), 2012 Mar 21; 23(3): 382-391.
Impact Factor: 4.58
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De Rosa L, Cortajarena AL, Romanelli A, Regan L, D'Andrea LD
* Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins (340 views)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2012; 10(2): 273-280.
Impact Factor: 3.568
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Napolitano M, Ottaiano A, Mauro F, Ieranò C, Satriano R, Pacelli R, Franco R, De Angelis V, Castello G, Scala S
* CD4(+)CD45RA(+)CXCR4(+) lymphocytes are inversely associated with progression in stages I-III melanoma patients (403 views)
Cancer Immunol Immunother (ISSN: 0340-7004, 0340-7004linking), 2010 Apr; 59(4): 511-517.
Impact Factor: 4.293
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Ciaccio C, Tundo GR, Grasso G, Spoto G, Marasco D, Ruvo M, Gioia M, Rizzarelli E, Coletta M
* Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity (455 views)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 Feb 6; 385(5): 1556-1567.
Impact Factor: 3.871
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Ziaco B, Pensato S, D'Andrea LD, Benedetti E, Romanelli A
* Semisynthesis of dimeric proteins by expressed protein ligation (310 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2008 May 15; 10(10): 1955-1958.
Impact Factor: 5.128
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Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (330 views)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
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* DNA Binding Mode Analysis of a Core-Extended Naphthalene Diimide as a Conformation-Sensitive Fluorescent Probe of G-Quadruplex Structures (10 views)
Int J Mol Sci (ISSN: 1422-0067linking), 2021 Sep 30; 22 (19 ): N/D-N/D.
Impact Factor:
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De Rosa L, Di Stasi R, Longhitano L, D'Andrea LD
* Labeling of VEGFR1D2 through oxime ligation (198 views)
Bioorg Chem (ISSN: 0045-2068linking, 1090-2120electronic), 2019 Jul 29; 91: 103160-103160.
Impact Factor: 3.926
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Sciacca MFM, Monaco I, La Rosa C, Milardi D
* The active role of Ca(2+) ions in Abeta-mediated membrane damage (325 views)
Chem Commun (ISSN: 1364-548xelectronic, 1359-7345linking), 2018 Apr 5; 54(29): 3629-3631.
Impact Factor: 6.169
View Export to BibTeX Export to EndNote
Avitabile C, Diaferia C, Della Ventura B, Mercurio FA, Leone M, Roviello V, Saviano M, Velotta R, Morelli G, Accardo A, Romanelli A
* Self-Assembling of Fmoc-GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates (891 views)
Chemistry (ISSN: 1521-3765electronic, 0947-6539linking), 2018 Mar 26; 24(18): 4729-4735.
Impact Factor: 5.476
View Export to BibTeX Export to EndNote
Deiana M, Mettra B, Martinez-fernandez L, Mazur LM, Pawlik K, Andraud C, Samoc M, Improta R, Monnereau C, Matczyszyn K
* Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe (415 views)
J Phys Chem Lett (ISSN: 1948-7185, 1948-7185electronic, 1948-7185linking), 2017 Dec 7; 8(23): 5915-5920.
Impact Factor: 8.709
View Export to BibTeX Export to EndNote
Santagata S, Portella L, Napolitano M, Greco A, D'Alterio C, Barone MV, Luciano A, Gramanzini M, Auletta L, Arra C, Zannetti A, Scala S
* A novel CXCR4-targeted near-infrared (NIR) fluorescent probe (Peptide R-NIR750) specifically detects CXCR4 expressing tumors (386 views) (PDF 206 views)
Sci Rep (ISSN: 2045-2322, 2045-2322electronic, 2045-2322linking), 2017 May 31; 7(1): 2554-2554.
Impact Factor: 4.122
View Export to BibTeX Export to EndNote
Borchmann DE, Tarallo R, Avendano S, Falanga A, Carberry TP, Galdiero S, Weck M
* Membranotropic peptide-functionalized poly(lactide)- graft -poly(ethylene glycol) brush copolymers for intracellular delivery (434 views)
Macromolecules (ISSN: 0024-9297), 2015; 48(4): 942-949.
Impact Factor: 5.554
View Export to BibTeX Export to EndNote
Giuffrida ML, Rizzarelli E, Tomaselli GA, Satriano C, Trusso Sfrazzetto G
* A novel fully water-soluble Cu(i) probe for fluorescence live cell imaging (375 views)
Chem Commun (ISSN: 1359-7345, 1364-548x, 1364-548xelectronic), 2014; 50(69): 9835-9838.
Impact Factor: 6.834
View Export to BibTeX Export to EndNote
Doti N, Raimondo D, Sabatella M, Ruvo M
* Identification of Protease Inhibitors by a Fast Fluorimetric Assay (683 views)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2013 Jun; 54(2): 283-291.
Impact Factor: 2.275
View Export to BibTeX Export to EndNote
Arena G, Attanasio F, Zhang D, Yang Y, Bartsch RA, Sgarlata C
* Selective sensing of Hg2+ by a proton-ionizable calix[4]arene fluoroionophore (347 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Jan; 405(2-3): 1133-1137.
Impact Factor: 3.578
View Export to BibTeX Export to EndNote
Pica A, Russo Krauss I, Castellano I, Rossi M, La Cara F, Graziano G, Sica F, Merlino A
* Exploring The Unfolding Mechanism Of Gamma-Glutamyltranspeptidases: The Case Of The Thermophilic Enzyme From Geobacillus Thermodenitrificans (566 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2012 Apr; 1824(4): 571-577.
Impact Factor: 3.733
View Export to BibTeX Export to EndNote
Musumeci D, Oliviero G, Roviello GN, Bucci EM, Piccialli G
* G-quadruplex-forming oligonucleotide conjugated to magnetic nanoparticles: Synthesis, characterization, and enzymatic stability assays (816 views)
Bioconjug Chem (ISSN: 1043-1802, 1520-4812, 1520-4812electronic), 2012 Mar 21; 23(3): 382-391.
Impact Factor: 4.58
View Export to BibTeX Export to EndNote
De Rosa L, Cortajarena AL, Romanelli A, Regan L, D'Andrea LD
* Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins (340 views)
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2012; 10(2): 273-280.
Impact Factor: 3.568
View Export to BibTeX Export to EndNote
Napolitano M, Ottaiano A, Mauro F, Ieranò C, Satriano R, Pacelli R, Franco R, De Angelis V, Castello G, Scala S
* CD4(+)CD45RA(+)CXCR4(+) lymphocytes are inversely associated with progression in stages I-III melanoma patients (403 views)
Cancer Immunol Immunother (ISSN: 0340-7004, 0340-7004linking), 2010 Apr; 59(4): 511-517.
Impact Factor: 4.293
View Export to BibTeX Export to EndNote
Ciaccio C, Tundo GR, Grasso G, Spoto G, Marasco D, Ruvo M, Gioia M, Rizzarelli E, Coletta M
* Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity (455 views)
J Mol Biol (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 Feb 6; 385(5): 1556-1567.
Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Ziaco B, Pensato S, D'Andrea LD, Benedetti E, Romanelli A
* Semisynthesis of dimeric proteins by expressed protein ligation (310 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2008 May 15; 10(10): 1955-1958.
Impact Factor: 5.128
View Export to BibTeX Export to EndNote
Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (330 views)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
View Export to BibTeX Export to EndNote
17 Records (17 excluding Abstracts).
Total impact factor: 74.849 (74.849 excluding Abstracts).
Total 5 year impact factor: 74.052 (74.052 excluding Abstracts).
Total impact factor: 74.849 (74.849 excluding Abstracts).
Total 5 year impact factor: 74.052 (74.052 excluding Abstracts).
468 views. Last view on Sunday 14 May 2023, 8:16:52
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