Semi-Synthesis of Labeled Proteins for Spectroscopic Applications (546 views)
Molecules (ISSN: 1420-3049, 1420-3049electronic, 1420-3049linking), 2013 Jan; 18(1): 440-465.
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Paper type: Journal Article,
Impact factor: 2.095, 5-year impact factor: 2.638
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872854831&partnerID=40&md5=ca926f404bd6ff70e014005aa0d6e5b1
Keywords: Expressed Protein Ligation, Förster Resonance Energy Transfer, Intein, Protein Labeling, Segmental Labeling, Fluorescent Dye, Peptide Fragment, Recombinant Protein, Biosynthesis, Chemistry, Fluorescence Resonance Energy Transfer, Genetics, Human, Isotope Labeling, Methodology, Nuclear Magnetic Resonance, Protein Engineering, Protein Motif, Review, Staining, Amino Acid Motifs, Biomolecular, Staining And Labeling, F Rster Resonance Energy Transfer,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy
Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, Via Mezzocannone 16, Napoli 80134, Italy
References:
Not available.
Molecules (ISSN: 1420-3049, 1420-3049electronic, 1420-3049linking), 2013 Jan; 18(1): 440-465.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.095, 5-year impact factor: 2.638
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872854831&partnerID=40&md5=ca926f404bd6ff70e014005aa0d6e5b1
Keywords: Expressed Protein Ligation, Förster Resonance Energy Transfer, Intein, Protein Labeling, Segmental Labeling, Fluorescent Dye, Peptide Fragment, Recombinant Protein, Biosynthesis, Chemistry, Fluorescence Resonance Energy Transfer, Genetics, Human, Isotope Labeling, Methodology, Nuclear Magnetic Resonance, Protein Engineering, Protein Motif, Review, Staining, Amino Acid Motifs, Biomolecular, Staining And Labeling, F Rster Resonance Energy Transfer,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy
Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, Via Mezzocannone 16, Napoli 80134, Italy
References:
Not available.
Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes. © 2013 by the authors.
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes. © 2013 by the authors.
Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
| ▼ Chemical protein synthesis and modification for biomedical applications |
Semi-Synthesis of Labeled Proteins for Spectroscopic Applications
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Impact Factor: 2.598
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Impact Factor: 3.926
View Export to BibTeX Export to EndNote
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Impact Factor: 5.476
View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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Impact Factor: 5.554
View Export to BibTeX Export to EndNote
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Impact Factor: 2.275
View Export to BibTeX Export to EndNote
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Impact Factor: 3.578
View Export to BibTeX Export to EndNote
Pica A, Russo Krauss I, Castellano I, Rossi M, La Cara F, Graziano G, Sica F, Merlino A
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Impact Factor: 3.733
View Export to BibTeX Export to EndNote
Musumeci D, Oliviero G, Roviello GN, Bucci EM, Piccialli G
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Bioconjug Chem (ISSN: 1043-1802, 1520-4812, 1520-4812electronic), 2012 Mar 21; 23(3): 382-391.
Impact Factor: 4.58
View Export to BibTeX Export to EndNote
De Rosa L, Cortajarena AL, Romanelli A, Regan L, D'Andrea LD
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Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2012; 10(2): 273-280.
Impact Factor: 3.568
View Export to BibTeX Export to EndNote
Napolitano M, Ottaiano A, Mauro F, Ieranò C, Satriano R, Pacelli R, Franco R, De Angelis V, Castello G, Scala S
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Impact Factor: 4.293
View Export to BibTeX Export to EndNote
Ciaccio C, Tundo GR, Grasso G, Spoto G, Marasco D, Ruvo M, Gioia M, Rizzarelli E, Coletta M
* Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity (477 views)
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Impact Factor: 3.871
View Export to BibTeX Export to EndNote
Ziaco B, Pensato S, D'Andrea LD, Benedetti E, Romanelli A
* Semisynthesis of dimeric proteins by expressed protein ligation (318 views)
Org Lett (ISSN: 1523-7060, 1523-7052, 1523-7052electronic), 2008 May 15; 10(10): 1955-1958.
Impact Factor: 5.128
View Export to BibTeX Export to EndNote
Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (364 views)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
View Export to BibTeX Export to EndNote
17 Records (17 excluding Abstracts).
Total impact factor: 77.447 (77.447 excluding Abstracts).
Total 5 year impact factor: 76.669 (76.669 excluding Abstracts).
Total impact factor: 77.447 (77.447 excluding Abstracts).
Total 5 year impact factor: 76.669 (76.669 excluding Abstracts).
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