Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion (336 views)
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220electronic), 2013; 13(1): 500-515.
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Paper type: Journal Article,
Impact factor: 2.048, 5-year impact factor: 2.457
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872966405&partnerID=40&md5=97d6a25a9aa3b6c330a789a0e3735eaa
Keywords: Hindlimb Perfusion, Laser Doppler Perfusion Imaging, Mice, Microcirculation, Standardisation, Experimental Conditions, Experimental Groups, Hind Limbs, Laser Doppler Imaging, Significant Differences, Skin Microcirculation, Diagnosis, Disease Control, Doppler Effect, Drug Therapy, Imaging Techniques, Isomers, Laser Doppler Velocimeters, Standardization, Mammals, Animal, Article, Female, Human, Laser Doppler Flowmetry, Methodology, Mouse, Physiology, Reproducibility, Vascularization, Laser-Doppler Flowmetry, Reference Standards, Reproducibility Of Results, Blood Supply,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Biomediche Avanzate, University Federico II, Via Pansini 5, 8013 Naples, Italy. adegreco@unina.it
CEINGE, Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145 Naples, Italy
Fondazione IRCCS SDN, Instituto per la Ricerca e l'alta Formazione in Diagnostica Nucleare, Via Gianturco 113, 80143 Naples, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via Tommaso De Amicis 95, 80145 Naples, Italy
E-Mails: sandralbanese@gmail.com (S.A.)
marsalva@unina.it (M.S.)
brunetti@unina.it (A.B
References:
Paigen, K., Understanding the human condition: Experimental strategies in mammalian genetics (2002) ILAR J., 3, pp. 123-13
Condeelis, J., Weissleder, R., In vivo imaging in cancer (2010) Cold Spring Harb. Perspect. Biol.
Massoud, T.F., Gambhir, S.S., Molecular imaging in living subjects: Seeing fundamental biological processes in a new light (2003) Genes Dev., 5, pp. 545-580
Sarnik, S., Hofirek, I., Sochor, O., Laser Doppler fluxmetry (2007) Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 1, pp. 143-146
(2012) Laser Doppler Flowmetry-A Theoretical Framework, , http://www.imt.liu.se/bit/ldf/ldfmain.html, Available online, (accessed on 20 December)
Key, H., Jackson, P.C., Wells, P.N., New approaches to transillumination imaging (1988) J. Biomed. Eng., 2, pp. 113-118
Humeau, A., Steenbergen, W., Nilsson, H., Strömberg, T., Laser Doppler perfusion monitoring and imaging: Novel approaches (2007) Med. Biol. Eng. Comput., 5, pp. 421-435
Niiyama, H., Huang, N.F., Rollins, M.D., Cooke, J.P., Murine model of hindlimb ischemia (2009) J. Vis. Exp.
Rayssac, A., Neveu, C., Pucelle, M., van den Berghe, L., Prado-Lourenco, L., Arnal, J.F., Chaufour, X., Prats, A.C., IRES-based vector coexpressing FGF2 and Cyr61 provides synergistic and safe therapeutics of lower limb ischemia (2009) Mol. Ther., 12, pp. 2010-2019
Cho, W.G., Albuquerque, R.J., Kleinman, M.E., Tarallo, V., Greco, A., Nozaki, M., Green, M.G., De Falco, M., Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth (2009) PNAS, 17, pp. 7137-7142
Kragh, M., Quistorff, B., Kristjansen, P.E.G., Quantitative estimates of angiogenic and anti-angiogenic activity by laser Doppler flowmetry (LDF) and near infra-red spectroscopy (NIRS) (2001) Eur. J. Cancer, 37, pp. 924-929
(2011) National Research Council. Guide for the Care and Use of Laboratory Animals, , Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 8th ed.
The National Academies Press: Washington, DC, USA
Bland, J.M., Altman, D.G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 327, pp. 307-310
Yang, X.P., Liu, Y.H., Rhaleb, N.E., Kurihara, N., Kim, H.E., Carrettero, O., Echocardiographic assessment of cardiac function in conscious and anesthetized mice (1999) Am. J. Physiol. Heart Circ. Physiol., 277, pp. H1967-H1974
Constantinides, C., Mean, R., Janssen, B.J., Effects of isoflurane anesthesia on the cardiovascular function of the C57BL/6 mouse (2011) ILAR J., 52, pp. 21-31
Gargiulo, S., Greco, A., Gramanzini, M., Esposito, S., Affuso, A., Brunetti, A., Vesce, G., Mice anesthesia, analgesia, and care, part II: Special considerations for preclinical imaging studies (2012) ILAR J., , http://nas-sites.org/ilarjournal/current-issue/neurobiology-ofaddictive- behaviors/mice-anesthesia-analgesia-and-care-part-ii-special-considerati ons-for-preclinicalimaging-studies/, Available online, (accessed on 27 December 2012)
Michauld, S.E., Menard, C., Guy, L.G., Gennaro, G., Rivard, A., Inhibition of hypoxia-induced angiogenesis by cigarette smoke exposure: Impairment of the HIF-1alpha/VEGF pathway (2003) FASEB J., 10, p. 1096
Massoud, T. F., Gambhir, S. S., Molecular imaging in living subjects: Seeing fundamental biological processes in a new light (2003) Genes Dev., 5, pp. 545-580
(2012) Laser Doppler Flowmetry-A Theoretical Framework, , http: //www. imt. liu. se/bit/ldf/ldfmain. html, Available online, (accessed on 20 December)
Cho, W. G., Albuquerque, R. J., Kleinman, M. E., Tarallo, V., Greco, A., Nozaki, M., Green, M. G., De Falco, M., Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth (2009) PNAS, 17, pp. 7137-7142
(2011) National Research Council. Guide for the Care and Use of Laboratory Animals, , Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 8th ed.
Bland, J. M., Altman, D. G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 327, pp. 307-310
Yang, X. P., Liu, Y. H., Rhaleb, N. E., Kurihara, N., Kim, H. E., Carrettero, O., Echocardiographic assessment of cardiac function in conscious and anesthetized mice (1999) Am. J. Physiol. Heart Circ. Physiol., 277, pp. H1967-H1974
Michauld, S. E., Menard, C., Guy, L. G., Gennaro, G., Rivard, A., Inhibition of hypoxia-induced angiogenesis by cigarette smoke exposure: Impairment of the HIF-1alpha/VEGF pathway (2003) FASEB J., 10, p. 1096
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220electronic), 2013; 13(1): 500-515.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.048, 5-year impact factor: 2.457
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872966405&partnerID=40&md5=97d6a25a9aa3b6c330a789a0e3735eaa
Keywords: Hindlimb Perfusion, Laser Doppler Perfusion Imaging, Mice, Microcirculation, Standardisation, Experimental Conditions, Experimental Groups, Hind Limbs, Laser Doppler Imaging, Significant Differences, Skin Microcirculation, Diagnosis, Disease Control, Doppler Effect, Drug Therapy, Imaging Techniques, Isomers, Laser Doppler Velocimeters, Standardization, Mammals, Animal, Article, Female, Human, Laser Doppler Flowmetry, Methodology, Mouse, Physiology, Reproducibility, Vascularization, Laser-Doppler Flowmetry, Reference Standards, Reproducibility Of Results, Blood Supply,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Biomediche Avanzate, University Federico II, Via Pansini 5, 8013 Naples, Italy. adegreco@unina.it
CEINGE, Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145 Naples, Italy
Fondazione IRCCS SDN, Instituto per la Ricerca e l'alta Formazione in Diagnostica Nucleare, Via Gianturco 113, 80143 Naples, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via Tommaso De Amicis 95, 80145 Naples, Italy
E-Mails: sandralbanese@gmail.com (S.A.)
marsalva@unina.it (M.S.)
brunetti@unina.it (A.B
References:
Paigen, K., Understanding the human condition: Experimental strategies in mammalian genetics (2002) ILAR J., 3, pp. 123-13
Condeelis, J., Weissleder, R., In vivo imaging in cancer (2010) Cold Spring Harb. Perspect. Biol.
Massoud, T.F., Gambhir, S.S., Molecular imaging in living subjects: Seeing fundamental biological processes in a new light (2003) Genes Dev., 5, pp. 545-580
Sarnik, S., Hofirek, I., Sochor, O., Laser Doppler fluxmetry (2007) Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 1, pp. 143-146
(2012) Laser Doppler Flowmetry-A Theoretical Framework, , http://www.imt.liu.se/bit/ldf/ldfmain.html, Available online, (accessed on 20 December)
Key, H., Jackson, P.C., Wells, P.N., New approaches to transillumination imaging (1988) J. Biomed. Eng., 2, pp. 113-118
Humeau, A., Steenbergen, W., Nilsson, H., Strömberg, T., Laser Doppler perfusion monitoring and imaging: Novel approaches (2007) Med. Biol. Eng. Comput., 5, pp. 421-435
Niiyama, H., Huang, N.F., Rollins, M.D., Cooke, J.P., Murine model of hindlimb ischemia (2009) J. Vis. Exp.
Rayssac, A., Neveu, C., Pucelle, M., van den Berghe, L., Prado-Lourenco, L., Arnal, J.F., Chaufour, X., Prats, A.C., IRES-based vector coexpressing FGF2 and Cyr61 provides synergistic and safe therapeutics of lower limb ischemia (2009) Mol. Ther., 12, pp. 2010-2019
Cho, W.G., Albuquerque, R.J., Kleinman, M.E., Tarallo, V., Greco, A., Nozaki, M., Green, M.G., De Falco, M., Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth (2009) PNAS, 17, pp. 7137-7142
Kragh, M., Quistorff, B., Kristjansen, P.E.G., Quantitative estimates of angiogenic and anti-angiogenic activity by laser Doppler flowmetry (LDF) and near infra-red spectroscopy (NIRS) (2001) Eur. J. Cancer, 37, pp. 924-929
(2011) National Research Council. Guide for the Care and Use of Laboratory Animals, , Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 8th ed.
The National Academies Press: Washington, DC, USA
Bland, J.M., Altman, D.G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 327, pp. 307-310
Yang, X.P., Liu, Y.H., Rhaleb, N.E., Kurihara, N., Kim, H.E., Carrettero, O., Echocardiographic assessment of cardiac function in conscious and anesthetized mice (1999) Am. J. Physiol. Heart Circ. Physiol., 277, pp. H1967-H1974
Constantinides, C., Mean, R., Janssen, B.J., Effects of isoflurane anesthesia on the cardiovascular function of the C57BL/6 mouse (2011) ILAR J., 52, pp. 21-31
Gargiulo, S., Greco, A., Gramanzini, M., Esposito, S., Affuso, A., Brunetti, A., Vesce, G., Mice anesthesia, analgesia, and care, part II: Special considerations for preclinical imaging studies (2012) ILAR J., , http://nas-sites.org/ilarjournal/current-issue/neurobiology-ofaddictive- behaviors/mice-anesthesia-analgesia-and-care-part-ii-special-considerati ons-for-preclinicalimaging-studies/, Available online, (accessed on 27 December 2012)
Michauld, S.E., Menard, C., Guy, L.G., Gennaro, G., Rivard, A., Inhibition of hypoxia-induced angiogenesis by cigarette smoke exposure: Impairment of the HIF-1alpha/VEGF pathway (2003) FASEB J., 10, p. 1096
Massoud, T. F., Gambhir, S. S., Molecular imaging in living subjects: Seeing fundamental biological processes in a new light (2003) Genes Dev., 5, pp. 545-580
(2012) Laser Doppler Flowmetry-A Theoretical Framework, , http: //www. imt. liu. se/bit/ldf/ldfmain. html, Available online, (accessed on 20 December)
Cho, W. G., Albuquerque, R. J., Kleinman, M. E., Tarallo, V., Greco, A., Nozaki, M., Green, M. G., De Falco, M., Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth (2009) PNAS, 17, pp. 7137-7142
(2011) National Research Council. Guide for the Care and Use of Laboratory Animals, , Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 8th ed.
Bland, J. M., Altman, D. G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 327, pp. 307-310
Yang, X. P., Liu, Y. H., Rhaleb, N. E., Kurihara, N., Kim, H. E., Carrettero, O., Echocardiographic assessment of cardiac function in conscious and anesthetized mice (1999) Am. J. Physiol. Heart Circ. Physiol., 277, pp. H1967-H1974
Michauld, S. E., Menard, C., Guy, L. G., Gennaro, G., Rivard, A., Inhibition of hypoxia-induced angiogenesis by cigarette smoke exposure: Impairment of the HIF-1alpha/VEGF pathway (2003) FASEB J., 10, p. 1096
Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
Background. Preclinical perfusion studies are useful for the improvement of diagnosis and therapy in dermatologic, cardiovascular and rheumatic human diseases. The Laser Doppler Perfusion Imaging (LDPI) technique has been used to evaluate superficial alterations of the skin microcirculation in surgically induced murine hindlimb ischemia. We assessed the reproducibility and the accuracy of LDPI acquisitions and identified several critical factors that could affect LDPI measurements in mice. Methods. Twenty mice were analysed. Statistical standardisation and a repeatability and reproducibility analysis were performed on mouse perfusion signals with respect to differences in body temperature, the presence or absence of hair, the type of anaesthesia used for LDPI measurements and the position of the mouse body. Results. We found excellent correlations among measurements made by the same operator (i.e., repeatability) under the same experimental conditions and by two different operators (i.e., reproducibility). A Bland-Altman analysis showed the absence of bias in repeatability (p = 0.29) or reproducibility (p = 0.89). The limits of agreement for repeatability were -0.357 and -0.033, and for reproducibility, they were -0.270 and 0.238. Significant differences in perfusion values were observed in different experimental groups. Conclusions. Different experimental conditions must be considered as a starting point for the evaluation of new drugs and strategic therapies. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Background. Preclinical perfusion studies are useful for the improvement of diagnosis and therapy in dermatologic, cardiovascular and rheumatic human diseases. The Laser Doppler Perfusion Imaging (LDPI) technique has been used to evaluate superficial alterations of the skin microcirculation in surgically induced murine hindlimb ischemia. We assessed the reproducibility and the accuracy of LDPI acquisitions and identified several critical factors that could affect LDPI measurements in mice. Methods. Twenty mice were analysed. Statistical standardisation and a repeatability and reproducibility analysis were performed on mouse perfusion signals with respect to differences in body temperature, the presence or absence of hair, the type of anaesthesia used for LDPI measurements and the position of the mouse body. Results. We found excellent correlations among measurements made by the same operator (i.e., repeatability) under the same experimental conditions and by two different operators (i.e., reproducibility). A Bland-Altman analysis showed the absence of bias in repeatability (p = 0.29) or reproducibility (p = 0.89). The limits of agreement for repeatability were -0.357 and -0.033, and for reproducibility, they were -0.270 and 0.238. Significant differences in perfusion values were observed in different experimental groups. Conclusions. Different experimental conditions must be considered as a starting point for the evaluation of new drugs and strategic therapies. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
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Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
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Bmc Veterinary Research (ISSN: 1746-6148, 1746-6614), 2013 Dec 17; 9: 255-255.
Impact Factor: 1.743
View Export to BibTeX Export to EndNote
Mancini M, Di Donato O, Saldalamacchia G, Liuzzi R, Rivellese A, Salvatore M
* Contrast-enhanced ultrasound evaluation of peripheral microcirculation in diabetic patients: effects of cigarette smoking (441 views)
Radiol Med (ISSN: 1826-6983, 0033-8362, 1826-6983electronic), 2013 Mar; 118(2): 206-214.
Impact Factor: 1.368
View Export to BibTeX Export to EndNote
Bellia F, Pietropaolo A, Grasso G
* Formation of insulin fragments by insulin-degrading enzyme: The role of zinc(II) and cystine bridges (460 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2013; 48(2): 135-140.
Impact Factor: 2.709
View Export to BibTeX Export to EndNote
D'Urso L, Grasso G, Messina E, Bongiorno C, Scuderi V, Scalese S, Puglisi O, Spoto G, Compagnini G
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5 Records (5 excluding Abstracts).
Total impact factor: 10.344 (10.344 excluding Abstracts).
Total 5 year impact factor: 11.199 (11.199 excluding Abstracts).
Total impact factor: 10.344 (10.344 excluding Abstracts).
Total 5 year impact factor: 11.199 (11.199 excluding Abstracts).
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