Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion (437 views)
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220electronic), 2013; 13(1): 500-515.
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Paper type: Journal Article,
Impact factor: 2.048, 5-year impact factor: 2.457
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872966405&partnerID=40&md5=97d6a25a9aa3b6c330a789a0e3735eaa
Keywords: Hindlimb Perfusion, Laser Doppler Perfusion Imaging, Mice, Microcirculation, Standardisation, Experimental Conditions, Experimental Groups, Hind Limbs, Laser Doppler Imaging, Significant Differences, Skin Microcirculation, Diagnosis, Disease Control, Doppler Effect, Drug Therapy, Imaging Techniques, Isomers, Laser Doppler Velocimeters, Standardization, Mammals, Animal, Article, Female, Human, Laser Doppler Flowmetry, Methodology, Mouse, Physiology, Reproducibility, Vascularization, Laser-Doppler Flowmetry, Reference Standards, Reproducibility Of Results, Blood Supply,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Biomediche Avanzate, University Federico II, Via Pansini 5, 8013 Naples, Italy. adegreco@unina.it
CEINGE, Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145 Naples, Italy
Fondazione IRCCS SDN, Instituto per la Ricerca e l'alta Formazione in Diagnostica Nucleare, Via Gianturco 113, 80143 Naples, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via Tommaso De Amicis 95, 80145 Naples, Italy
E-Mails: sandralbanese@gmail.com (S.A.)
marsalva@unina.it (M.S.)
brunetti@unina.it (A.B
References:
Not available.
Sensors (ISSN: 1424-8220, 1424-3210, 1424-8220electronic), 2013; 13(1): 500-515.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 2.048, 5-year impact factor: 2.457
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84872966405&partnerID=40&md5=97d6a25a9aa3b6c330a789a0e3735eaa
Keywords: Hindlimb Perfusion, Laser Doppler Perfusion Imaging, Mice, Microcirculation, Standardisation, Experimental Conditions, Experimental Groups, Hind Limbs, Laser Doppler Imaging, Significant Differences, Skin Microcirculation, Diagnosis, Disease Control, Doppler Effect, Drug Therapy, Imaging Techniques, Isomers, Laser Doppler Velocimeters, Standardization, Mammals, Animal, Article, Female, Human, Laser Doppler Flowmetry, Methodology, Mouse, Physiology, Reproducibility, Vascularization, Laser-Doppler Flowmetry, Reference Standards, Reproducibility Of Results, Blood Supply,
Affiliations:
*** IBB - CNR ***
Dipartimento di Scienze Biomediche Avanzate, University Federico II, Via Pansini 5, 8013 Naples, Italy. adegreco@unina.it
CEINGE, Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145 Naples, Italy
Fondazione IRCCS SDN, Instituto per la Ricerca e l'alta Formazione in Diagnostica Nucleare, Via Gianturco 113, 80143 Naples, Italy
Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via Tommaso De Amicis 95, 80145 Naples, Italy
E-Mails: sandralbanese@gmail.com (S.A.)
marsalva@unina.it (M.S.)
brunetti@unina.it (A.B
References:
Not available.
Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
Background. Preclinical perfusion studies are useful for the improvement of diagnosis and therapy in dermatologic, cardiovascular and rheumatic human diseases. The Laser Doppler Perfusion Imaging (LDPI) technique has been used to evaluate superficial alterations of the skin microcirculation in surgically induced murine hindlimb ischemia. We assessed the reproducibility and the accuracy of LDPI acquisitions and identified several critical factors that could affect LDPI measurements in mice. Methods. Twenty mice were analysed. Statistical standardisation and a repeatability and reproducibility analysis were performed on mouse perfusion signals with respect to differences in body temperature, the presence or absence of hair, the type of anaesthesia used for LDPI measurements and the position of the mouse body. Results. We found excellent correlations among measurements made by the same operator (i.e., repeatability) under the same experimental conditions and by two different operators (i.e., reproducibility). A Bland-Altman analysis showed the absence of bias in repeatability (p = 0.29) or reproducibility (p = 0.89). The limits of agreement for repeatability were -0.357 and -0.033, and for reproducibility, they were -0.270 and 0.238. Significant differences in perfusion values were observed in different experimental groups. Conclusions. Different experimental conditions must be considered as a starting point for the evaluation of new drugs and strategic therapies. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Background. Preclinical perfusion studies are useful for the improvement of diagnosis and therapy in dermatologic, cardiovascular and rheumatic human diseases. The Laser Doppler Perfusion Imaging (LDPI) technique has been used to evaluate superficial alterations of the skin microcirculation in surgically induced murine hindlimb ischemia. We assessed the reproducibility and the accuracy of LDPI acquisitions and identified several critical factors that could affect LDPI measurements in mice. Methods. Twenty mice were analysed. Statistical standardisation and a repeatability and reproducibility analysis were performed on mouse perfusion signals with respect to differences in body temperature, the presence or absence of hair, the type of anaesthesia used for LDPI measurements and the position of the mouse body. Results. We found excellent correlations among measurements made by the same operator (i.e., repeatability) under the same experimental conditions and by two different operators (i.e., reproducibility). A Bland-Altman analysis showed the absence of bias in repeatability (p = 0.29) or reproducibility (p = 0.89). The limits of agreement for repeatability were -0.357 and -0.033, and for reproducibility, they were -0.270 and 0.238. Significant differences in perfusion values were observed in different experimental groups. Conclusions. Different experimental conditions must be considered as a starting point for the evaluation of new drugs and strategic therapies. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
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Repeatability, Reproducibility and Standardisation of a Laser Doppler Imaging Technique for the Evaluation of Normal Mouse Hindlimb Perfusion
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* Effects Of Some Anesthetic Agents On Skin Microcirculation Evaluated By Laser Doppler Perfusion Imaging In Mice (576 views)
Bmc Veterinary Research (ISSN: 1746-6148, 1746-6614), 2013 Dec 17; 9: 255-255.
Impact Factor: 1.743
View Export to BibTeX Export to EndNote
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Impact Factor: 1.368
View Export to BibTeX Export to EndNote
Bellia F, Pietropaolo A, Grasso G
* Formation of insulin fragments by insulin-degrading enzyme: The role of zinc(II) and cystine bridges (590 views)
J Mass Spectrom (ISSN: 1076-5174, 1096-9888), 2013; 48(2): 135-140.
Impact Factor: 2.709
View Export to BibTeX Export to EndNote
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View Export to BibTeX Export to EndNote
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5 Records (5 excluding Abstracts).
Total impact factor: 10.344 (10.344 excluding Abstracts).
Total 5 year impact factor: 11.199 (11.199 excluding Abstracts).
Total impact factor: 10.344 (10.344 excluding Abstracts).
Total 5 year impact factor: 11.199 (11.199 excluding Abstracts).
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