Thymidine Kinase-Mediated Shut Down of Bone Morphogenetic Protein-4 Expression Allows Regulated Bone Production (411 views)
Current Gene Therapy (ISSN: 1566-5232, 1875-5631), 2013 Jun 1; 13(3): 202-210.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 4.906, 5-year impact factor: 4.087
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84878661287&partnerID=40&md5=d34b8cfd0d6edde8d279198bb905138e
Keywords: Bone Production Regulation, Fg-B4, Adenovirus Vector, Bone Morphogenetic Protein 4, Ganciclovir, Thymidine Kinase, Animal Experiment, Animal Tissue, Apoptosis, Article, Bone Densitometry, Cell Death, Cell Differentiation, Cell Infiltration, Controlled Study, Dual Energy X Ray Absorptiometry, Exostosis, Fibrosis, Histology, Human, Human Cell, Inflammation, Micro-Computed Tomography, Mouse, Nick End Labeling, Nonhuman, Ossification, Protein Expression, Upregulation, Bone And Bones, Bone Development, Gene Expression Regulation, Gene Transfer Techniques, Genetic Vectors, Nude, Osteogenesis, Quadriceps Muscle, Simplexvirus, Transgenes, Mus Musculus, Oryctolagus Cuniculus,
Affiliations:
*** IBB - CNR ***
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy
CEINGE-Biotecnologie Avanzate, Via Gaetano Salvatore 486, 80145 Napoli, Italy
Dipartimento di Scienze Biomorfologiche e Funzionali, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy
References:
Luther, G., Wagner, E.R., Zhu, G., BMP-9 Induced Osteogenic Differentiation of Mesenchymal Stem Cells: Molecular Mechanism and Therapeutic Potentia (2011) Curr Gene Ther, 11 (3), pp. 229-24
Wutzl, A., Rauner, M., Seemann, R., Bone morphogenetic proteins 2, 5, and 6 in combination stimulate osteoblasts but not osteoclasts in vitro (2010) J Orthop Res, 28 (11), pp. 1431-1439
Castro-Govea, Y., Cervantes-Kardasch, V.H., Borrego-Soto, G., Human bone morphogenetic protein 2-transduced mesenchymal stem cells improve bone regeneration in a model of mandible distraction surgery (2012) J Craniofac Surg, 23 (2), pp. 392-396
Garrison, K.R., Shemilt, I., Donell, S., Bone morphogenetic protein (BMP) for fracture healing in adults (2010) Cochrane Database Syst Rev, (6), pp. CD006950
Nauth, A., Ristevski, B., Li, R., Schemitsch, E.H., Growth factors and bone regeneration: How much bone can we expect? (2011) Injury, 42 (6), pp. 574-579
Rider, C.C., Mulloy, B., Bone morphogenetic protein and growth differentiation factor cytokine families and their protein antagonists (2010) Biochem J, 429 (1), pp. 1-12
Spiro, A.S., Beil, F.T., Schinke, T., Short-term application of dexamethasone enhances bone morphogenetic protein-7-induced ectopic bone formation in vivo (2011) J Trauma, 69 (6), pp. 1473-1480
Tachi, K., Takami, M., Sato, H., Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor-beta1 (2011) Tissue Eng Part A, 17 (5-6), pp. 597-606
El Tamer, M.K., Reis, R.L., Progenitor and stem cells for bone and cartilage regeneration (2009) J Tissue Eng Regen Med, 3 (5), pp. 327-337
Koh, J.T., Zhao, Z., Wang, Z., Lewis, I.S., Krebsbach, P.H., Franceschi, R.T., Combinatorial gene therapy with BMP2/7 enhances cranial bone regeneration (2008) J Dent Res, 87 (9), pp. 845-849
Khalafi, A., Schmid, T.M., Neu, C., Reddi, A.H., Increased accumulation of superficial zone protein (SZP) in articular cartilage in response to bone morphogenetic protein-7 and growth factors (2007) J Orthop Res, 25 (3), pp. 293-303
ten Dijke, P., Yamashita, H., Sampath, T.K., Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4 (1994) J Biol Chem, 269 (25), pp. 16985-16988
Heldin, C.H., Miyazono, K., ten Dijke, P., TGF-beta signalling from cell membrane to nucleus through SMAD proteins (1997) Nature, 390 (6659), pp. 465-471
Desai, P.P., Bell, A.J., Suk, M., Treatment of recalcitrant, multiply operated tibial nonunions with the RIA graft and rh-BMP2 using intramedullary nails (2010) Injury, 41 (SUPPL. 2), pp. S69-S71
Papanna, M.C., Al-Hadithy, N., Somanchi, B.V., The use of bone morphogenic protein-7 (OP-1) in the management of resistant nonunions in the upper and lower limb (2012) Injury, 43 (7), pp. 1135-1140
Li, J., Li, Y., Ma, S., Gao, Y., Zuo, Y., Hu, J., Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nanohydroxyapatite/ polyamide composite scaffolds in repair of mandibular defects (2010) J Biomed Mater Res A, 95 (4), pp. 973-981
Li, G., Berven, S., Simpson, H., Triffitt, J.T., Expression of BMP-4 mRNA during distraction osteogenesis in rabbits (1998) Acta Orthop Scand, 69 (4), pp. 420-425
Nakase, T., Nomura, S., Yoshikawa, H., Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing (1994) J Bone Miner Res, 9 (5), pp. 651-659
Zara, J.N., Siu, R.K., Zhang, X., High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo (2011) Tissue Eng Part A, 17 (9-10), pp. 1389-1399
Wubbenhorst, D., Dumler, K., Wagner, B., Tetracyclineregulated bone morphogenetic protein 2 gene expression in lentivirally transduced primary rabbit chondrocytes for treatment of cartilage defects (2010) Arthritis Rheum, 62 (7), pp. 2037-2046
Koh, J.T., Ge, C., Zhao, M., Use of a stringent dimerizerregulated gene expression system for controlled BMP2 delivery (2006) Mol Ther, 14 (5), pp. 684-691
Jiang, Y.X., Lu, Y., Liu, T.J., Yang, J., Chen, Y., Fang, Y.W., Using HSVTK/ GCV suicide gene therapy to inhibit lens epithelial cell proliferation for treatment of posterior capsular opacification (2011) Mol Vis, 17, pp. 291-299
Usas, A., Maciulaitis, J., Maciulaitis, R., Jakuboniene, N., Milasius, A., Huard, J., Skeletal muscle-derived stem cells: Implications for cellmediated therapies (2011) Medicina (Kaunas), 47 (9), pp. 469-479
Usas, A., Huard, J., Muscle-derived stem cells for tissue engineering and regenerative therapy (2007) Biomaterials, 28 (36), pp. 5401-5406
Peng, H., Usas, A., Gearhart, B., Olshanski, A., Shen, H.C., Huard, J., Converse relationship between in vitro osteogenic differentiation and in vivo bone healing elicited by different populations of muscle-derived cells genetically engineered to express BMP4 (2004) J Bone Miner Res, 19 (4), pp. 630-641
Shen, H.C., Peng, H., Usas, A., Ex vivo gene therapy-induced endochondral bone formation: Comparison of muscle-derived stem cells and different subpopulations of primary muscle-derived cells (2004) Bone, 34 (6), pp. 982-992
Usas, A., Ho, A.M., Cooper, G.M., Olshanski, A., Peng, H., Huard, J., Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system (2009) Tissue Eng Part A, 15 (2), pp. 285-293
Savarino, L., Baldini, N., Greco, M., The performance of polyepsilon-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4 (2007) Biomaterials, 28 (20), pp. 3101-3109
Graham, F.L., Growth of 293 cells in suspension culture (1987) J Gen Virol, 68 (Pt 3), pp. 937-940
Becker, T.C., Noel, R.J., Coats, W.S., Use of recombinant adenovirus for metabolic engineering of mammalian cells (1994) Methods Cell Biol, (43 PART A), pp. 161-189
Kempen, D.H., Lu, L., Hefferan, T.E., Enhanced bone morphogenetic protein-2-induced ectopic and orthotopic bone formation by intermittent parathyroid hormone (1-34) administration (2010) Tissue Eng Part A, 16 (12), pp. 3769-3777
Jung, M.R., Shim, I.K., Chung, H.J., Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits (2012) J Control Release, 162 (3), pp. 485-491
Li, B.C., Zhang, J.J., Xu, C., Zhang, L.C., Kang, J.Y., Zhao, H., Treatment of rabbit femoral defect by firearm with BMP-4 gene combined with TGF-beta1 (2009) J Trauma, 66 (2), pp. 450-456
Cook, S.D., Baffes, G.C., Wolfe, M.W., Sampath, T.K., Rueger, D.C., Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model (1994) Clin Orthop Relat Res, (301), pp. 302-312
Gerhart, T.N., Kirker-Head, C.A., Kriz, M.J., Healing segmental femoral defects in sheep using recombinant human bone morphogenetic protein (1993) Clin Orthop Relat Res, (293), pp. 317-326
Cook, S.D., Wolfe, M.W., Salkeld, S.L., Rueger, D.C., Effect of recombinant human osteogenic protein-1 on healing of segmental defects in non-human primates (1995) J Bone Joint Surg Am, 77 (5), pp. 734-750
Miljkovic, N.D., Cooper, G.M., Marra, K.G., Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells (2008) Osteoarthritis Cartilage, 16 (10), pp. 1121-1130
Griffin, M., Iqbal, S.A., Bayat, A., Exploring the application of mesenchymal stem cells in bone repair and regeneration (2011) J Bone Joint Surg Br, 93 (4), pp. 427-434
Kim, I.S., Cho, T.H., Lee, Z.H., Hwang, S.J., Bone Regeneration by Transplantation of Human Mesenchymal Stromal Cells in a Rabbit Mandibular Distraction Osteogenesis Model (2012) Tissue Eng Part A, 19 (1-2), pp. 66-78
Bruder, S.P., Jaiswal, N., Haynesworth, S.E., Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation (1997) J Cell Biochem, 64 (2), pp. 278-294
Esposito, M.T., Di Noto, R., Mirabelli, P., Culture conditions allow selection of different mesenchymal progenitors from adult mouse bone marrow (2009) Tissue Eng Part A, 15 (9), pp. 2525-2536
Xiang, L., Liang, C., Zhen-Yong, K., Liang-Jun, Y., Zhong-Liang, D., BMP9-induced osteogenetic differentiation and bone formation of muscle-derived stem cells (2012) J Biomed Biotechnol, p. 610952
Bikram, M., Fouletier-Dilling, C., Hipp, J.A., Endochondral bone formation from hydrogel carriers loaded with BMP2-transduced cells (2007) Ann Biomed Eng, 35 (5), pp. 796-807
Payumo, F.C., Kim, H.D., Sherling, M.A., Tissue engineering skeletal muscle for orthopaedic applications (2002) Clin Orthop Relat Res, (403 SUPPL.), pp. S228-S242
Desurmont, C., Caillaud, J.M., Emmanuel, F., Complete atherosclerosis regression after human ApoE gene transfer in ApoEdeficient/ nude mice (2000) Arterioscler Thromb Vasc Biol, 20 (2), pp. 435-442
Liu, B., Wang, N.N., Wang, Z.L., Improved nude mouse models for green fluorescence human endometriosis (2010) J Obstet Gynaecol Res, 36 (6), pp. 1214-1221
Kanaya, N., Yazawa, M., Goto-Koshino, Y., Anti-tumor effect of adenoviral vector-mediated p53 gene transfer on the growth of canine osteosarcoma xenografts in nude mice (2011) J Vet Med Sci, 73 (7), pp. 877-883
Chang, P.C., Cirelli, J.A., Jin, Q., Adenovirus encoding human platelet-derived growth factor-B delivered to alveolar bone defects exhibits safety and biodistribution profiles favorable for clinical use (2009) Hum Gene Ther, 20 (5), pp. 486-496
Hu, W.W., Ward, B.B., Wang, Z., Krebsbach, P.H., Bone regeneration in defects compromised by radiotherapy (2010) J Dent Res, 89 (1), pp. 77-81
Luo, T., Zhang, W., Shi, B., Cheng, X., Zhang, Y., Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery (2012) Clin Oral Implants Res, 23 (4), pp. 467-473
Garrison, K. R., Shemilt, I., Donell, S., Bone morphogenetic protein (BMP) for fracture healing in adults (2010) Cochrane Database Syst Rev, (6), pp. CD006950
Rider, C. C., Mulloy, B., Bone morphogenetic protein and growth differentiation factor cytokine families and their protein antagonists (2010) Biochem J, 429 (1), pp. 1-12
Spiro, A. S., Beil, F. T., Schinke, T., Short-term application of dexamethasone enhances bone morphogenetic protein-7-induced ectopic bone formation in vivo (2011) J Trauma, 69 (6), pp. 1473-1480
El Tamer, M. K., Reis, R. L., Progenitor and stem cells for bone and cartilage regeneration (2009) J Tissue Eng Regen Med, 3 (5), pp. 327-337
Koh, J. T., Zhao, Z., Wang, Z., Lewis, I. S., Krebsbach, P. H., Franceschi, R. T., Combinatorial gene therapy with BMP2/7 enhances cranial bone regeneration (2008) J Dent Res, 87 (9), pp. 845-849
Heldin, C. H., Miyazono, K., ten Dijke, P., TGF-beta signalling from cell membrane to nucleus through SMAD proteins (1997) Nature, 390 (6659), pp. 465-471
Desai, P. P., Bell, A. J., Suk, M., Treatment of recalcitrant, multiply operated tibial nonunions with the RIA graft and rh-BMP2 using intramedullary nails (2010) Injury, 41 (SUPPL. 2), pp. S69-S71
Papanna, M. C., Al-Hadithy, N., Somanchi, B. V., The use of bone morphogenic protein-7 (OP-1) in the management of resistant nonunions in the upper and lower limb (2012) Injury, 43 (7), pp. 1135-1140
Zara, J. N., Siu, R. K., Zhang, X., High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo (2011) Tissue Eng Part A, 17 (9-10), pp. 1389-1399
Koh, J. T., Ge, C., Zhao, M., Use of a stringent dimerizerregulated gene expression system for controlled BMP2 delivery (2006) Mol Ther, 14 (5), pp. 684-691
Jiang, Y. X., Lu, Y., Liu, T. J., Yang, J., Chen, Y., Fang, Y. W., Using HSVTK/ GCV suicide gene therapy to inhibit lens epithelial cell proliferation for treatment of posterior capsular opacification (2011) Mol Vis, 17, pp. 291-299
Shen, H. C., Peng, H., Usas, A., Ex vivo gene therapy-induced endochondral bone formation: Comparison of muscle-derived stem cells and different subpopulations of primary muscle-derived cells (2004) Bone, 34 (6), pp. 982-992
Usas, A., Ho, A. M., Cooper, G. M., Olshanski, A., Peng, H., Huard, J., Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system (2009) Tissue Eng Part A, 15 (2), pp. 285-293
Graham, F. L., Growth of 293 cells in suspension culture (1987) J Gen Virol, 68 (Pt 3), pp. 937-940
Becker, T. C., Noel, R. J., Coats, W. S., Use of recombinant adenovirus for metabolic engineering of mammalian cells (1994) Methods Cell Biol, (43 PART A), pp. 161-189
Kempen, D. H., Lu, L., Hefferan, T. E., Enhanced bone morphogenetic protein-2-induced ectopic and orthotopic bone formation by intermittent parathyroid hormone (1-34) administration (2010) Tissue Eng Part A, 16 (12), pp. 3769-3777
Jung, M. R., Shim, I. K., Chung, H. J., Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits (2012) J Control Release, 162 (3), pp. 485-491
Li, B. C., Zhang, J. J., Xu, C., Zhang, L. C., Kang, J. Y., Zhao, H., Treatment of rabbit femoral defect by firearm with BMP-4 gene combined with TGF-beta1 (2009) J Trauma, 66 (2), pp. 450-456
Cook, S. D., Baffes, G. C., Wolfe, M. W., Sampath, T. K., Rueger, D. C., Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model (1994) Clin Orthop Relat Res, (301), pp. 302-312
Gerhart, T. N., Kirker-Head, C. A., Kriz, M. J., Healing segmental femoral defects in sheep using recombinant human bone morphogenetic protein (1993) Clin Orthop Relat Res, (293), pp. 317-326
Cook, S. D., Wolfe, M. W., Salkeld, S. L., Rueger, D. C., Effect of recombinant human osteogenic protein-1 on healing of segmental defects in non-human primates (1995) J Bone Joint Surg Am, 77 (5), pp. 734-750
Miljkovic, N. D., Cooper, G. M., Marra, K. G., Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells (2008) Osteoarthritis Cartilage, 16 (10), pp. 1121-1130
Kim, I. S., Cho, T. H., Lee, Z. H., Hwang, S. J., Bone Regeneration by Transplantation of Human Mesenchymal Stromal Cells in a Rabbit Mandibular Distraction Osteogenesis Model (2012) Tissue Eng Part A, 19 (1-2), pp. 66-78
Bruder, S. P., Jaiswal, N., Haynesworth, S. E., Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation (1997) J Cell Biochem, 64 (2), pp. 278-294
Esposito, M. T., Di Noto, R., Mirabelli, P., Culture conditions allow selection of different mesenchymal progenitors from adult mouse bone marrow (2009) Tissue Eng Part A, 15 (9), pp. 2525-2536
Payumo, F. C., Kim, H. D., Sherling, M. A., Tissue engineering skeletal muscle for orthopaedic applications (2002) Clin Orthop Relat Res, (403 SUPPL.), pp. S228-S242
Liu, B., Wang, N. N., Wang, Z. L., Improved nude mouse models for green fluorescence human endometriosis (2010) J Obstet Gynaecol Res, 36 (6), pp. 1214-1221
Chang, P. C., Cirelli, J. A., Jin, Q., Adenovirus encoding human platelet-derived growth factor-B delivered to alveolar bone defects exhibits safety and biodistribution profiles favorable for clinical use (2009) Hum Gene Ther, 20 (5), pp. 486-496
Hu, W. W., Ward, B. B., Wang, Z., Krebsbach, P. H., Bone regeneration in defects compromised by radiotherapy (2010) J Dent Res, 89 (1), pp. 77-81
Current Gene Therapy (ISSN: 1566-5232, 1875-5631), 2013 Jun 1; 13(3): 202-210.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 4.906, 5-year impact factor: 4.087
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84878661287&partnerID=40&md5=d34b8cfd0d6edde8d279198bb905138e
Keywords: Bone Production Regulation, Fg-B4, Adenovirus Vector, Bone Morphogenetic Protein 4, Ganciclovir, Thymidine Kinase, Animal Experiment, Animal Tissue, Apoptosis, Article, Bone Densitometry, Cell Death, Cell Differentiation, Cell Infiltration, Controlled Study, Dual Energy X Ray Absorptiometry, Exostosis, Fibrosis, Histology, Human, Human Cell, Inflammation, Micro-Computed Tomography, Mouse, Nick End Labeling, Nonhuman, Ossification, Protein Expression, Upregulation, Bone And Bones, Bone Development, Gene Expression Regulation, Gene Transfer Techniques, Genetic Vectors, Nude, Osteogenesis, Quadriceps Muscle, Simplexvirus, Transgenes, Mus Musculus, Oryctolagus Cuniculus,
Affiliations:
*** IBB - CNR ***
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy
CEINGE-Biotecnologie Avanzate, Via Gaetano Salvatore 486, 80145 Napoli, Italy
Dipartimento di Scienze Biomorfologiche e Funzionali, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy
References:
Luther, G., Wagner, E.R., Zhu, G., BMP-9 Induced Osteogenic Differentiation of Mesenchymal Stem Cells: Molecular Mechanism and Therapeutic Potentia (2011) Curr Gene Ther, 11 (3), pp. 229-24
Wutzl, A., Rauner, M., Seemann, R., Bone morphogenetic proteins 2, 5, and 6 in combination stimulate osteoblasts but not osteoclasts in vitro (2010) J Orthop Res, 28 (11), pp. 1431-1439
Castro-Govea, Y., Cervantes-Kardasch, V.H., Borrego-Soto, G., Human bone morphogenetic protein 2-transduced mesenchymal stem cells improve bone regeneration in a model of mandible distraction surgery (2012) J Craniofac Surg, 23 (2), pp. 392-396
Garrison, K.R., Shemilt, I., Donell, S., Bone morphogenetic protein (BMP) for fracture healing in adults (2010) Cochrane Database Syst Rev, (6), pp. CD006950
Nauth, A., Ristevski, B., Li, R., Schemitsch, E.H., Growth factors and bone regeneration: How much bone can we expect? (2011) Injury, 42 (6), pp. 574-579
Rider, C.C., Mulloy, B., Bone morphogenetic protein and growth differentiation factor cytokine families and their protein antagonists (2010) Biochem J, 429 (1), pp. 1-12
Spiro, A.S., Beil, F.T., Schinke, T., Short-term application of dexamethasone enhances bone morphogenetic protein-7-induced ectopic bone formation in vivo (2011) J Trauma, 69 (6), pp. 1473-1480
Tachi, K., Takami, M., Sato, H., Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor-beta1 (2011) Tissue Eng Part A, 17 (5-6), pp. 597-606
El Tamer, M.K., Reis, R.L., Progenitor and stem cells for bone and cartilage regeneration (2009) J Tissue Eng Regen Med, 3 (5), pp. 327-337
Koh, J.T., Zhao, Z., Wang, Z., Lewis, I.S., Krebsbach, P.H., Franceschi, R.T., Combinatorial gene therapy with BMP2/7 enhances cranial bone regeneration (2008) J Dent Res, 87 (9), pp. 845-849
Khalafi, A., Schmid, T.M., Neu, C., Reddi, A.H., Increased accumulation of superficial zone protein (SZP) in articular cartilage in response to bone morphogenetic protein-7 and growth factors (2007) J Orthop Res, 25 (3), pp. 293-303
ten Dijke, P., Yamashita, H., Sampath, T.K., Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4 (1994) J Biol Chem, 269 (25), pp. 16985-16988
Heldin, C.H., Miyazono, K., ten Dijke, P., TGF-beta signalling from cell membrane to nucleus through SMAD proteins (1997) Nature, 390 (6659), pp. 465-471
Desai, P.P., Bell, A.J., Suk, M., Treatment of recalcitrant, multiply operated tibial nonunions with the RIA graft and rh-BMP2 using intramedullary nails (2010) Injury, 41 (SUPPL. 2), pp. S69-S71
Papanna, M.C., Al-Hadithy, N., Somanchi, B.V., The use of bone morphogenic protein-7 (OP-1) in the management of resistant nonunions in the upper and lower limb (2012) Injury, 43 (7), pp. 1135-1140
Li, J., Li, Y., Ma, S., Gao, Y., Zuo, Y., Hu, J., Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nanohydroxyapatite/ polyamide composite scaffolds in repair of mandibular defects (2010) J Biomed Mater Res A, 95 (4), pp. 973-981
Li, G., Berven, S., Simpson, H., Triffitt, J.T., Expression of BMP-4 mRNA during distraction osteogenesis in rabbits (1998) Acta Orthop Scand, 69 (4), pp. 420-425
Nakase, T., Nomura, S., Yoshikawa, H., Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing (1994) J Bone Miner Res, 9 (5), pp. 651-659
Zara, J.N., Siu, R.K., Zhang, X., High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo (2011) Tissue Eng Part A, 17 (9-10), pp. 1389-1399
Wubbenhorst, D., Dumler, K., Wagner, B., Tetracyclineregulated bone morphogenetic protein 2 gene expression in lentivirally transduced primary rabbit chondrocytes for treatment of cartilage defects (2010) Arthritis Rheum, 62 (7), pp. 2037-2046
Koh, J.T., Ge, C., Zhao, M., Use of a stringent dimerizerregulated gene expression system for controlled BMP2 delivery (2006) Mol Ther, 14 (5), pp. 684-691
Jiang, Y.X., Lu, Y., Liu, T.J., Yang, J., Chen, Y., Fang, Y.W., Using HSVTK/ GCV suicide gene therapy to inhibit lens epithelial cell proliferation for treatment of posterior capsular opacification (2011) Mol Vis, 17, pp. 291-299
Usas, A., Maciulaitis, J., Maciulaitis, R., Jakuboniene, N., Milasius, A., Huard, J., Skeletal muscle-derived stem cells: Implications for cellmediated therapies (2011) Medicina (Kaunas), 47 (9), pp. 469-479
Usas, A., Huard, J., Muscle-derived stem cells for tissue engineering and regenerative therapy (2007) Biomaterials, 28 (36), pp. 5401-5406
Peng, H., Usas, A., Gearhart, B., Olshanski, A., Shen, H.C., Huard, J., Converse relationship between in vitro osteogenic differentiation and in vivo bone healing elicited by different populations of muscle-derived cells genetically engineered to express BMP4 (2004) J Bone Miner Res, 19 (4), pp. 630-641
Shen, H.C., Peng, H., Usas, A., Ex vivo gene therapy-induced endochondral bone formation: Comparison of muscle-derived stem cells and different subpopulations of primary muscle-derived cells (2004) Bone, 34 (6), pp. 982-992
Usas, A., Ho, A.M., Cooper, G.M., Olshanski, A., Peng, H., Huard, J., Bone regeneration mediated by BMP4-expressing muscle-derived stem cells is affected by delivery system (2009) Tissue Eng Part A, 15 (2), pp. 285-293
Savarino, L., Baldini, N., Greco, M., The performance of polyepsilon-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4 (2007) Biomaterials, 28 (20), pp. 3101-3109
Graham, F.L., Growth of 293 cells in suspension culture (1987) J Gen Virol, 68 (Pt 3), pp. 937-940
Becker, T.C., Noel, R.J., Coats, W.S., Use of recombinant adenovirus for metabolic engineering of mammalian cells (1994) Methods Cell Biol, (43 PART A), pp. 161-189
Kempen, D.H., Lu, L., Hefferan, T.E., Enhanced bone morphogenetic protein-2-induced ectopic and orthotopic bone formation by intermittent parathyroid hormone (1-34) administration (2010) Tissue Eng Part A, 16 (12), pp. 3769-3777
Jung, M.R., Shim, I.K., Chung, H.J., Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits (2012) J Control Release, 162 (3), pp. 485-491
Li, B.C., Zhang, J.J., Xu, C., Zhang, L.C., Kang, J.Y., Zhao, H., Treatment of rabbit femoral defect by firearm with BMP-4 gene combined with TGF-beta1 (2009) J Trauma, 66 (2), pp. 450-456
Cook, S.D., Baffes, G.C., Wolfe, M.W., Sampath, T.K., Rueger, D.C., Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model (1994) Clin Orthop Relat Res, (301), pp. 302-312
Gerhart, T.N., Kirker-Head, C.A., Kriz, M.J., Healing segmental femoral defects in sheep using recombinant human bone morphogenetic protein (1993) Clin Orthop Relat Res, (293), pp. 317-326
Cook, S.D., Wolfe, M.W., Salkeld, S.L., Rueger, D.C., Effect of recombinant human osteogenic protein-1 on healing of segmental defects in non-human primates (1995) J Bone Joint Surg Am, 77 (5), pp. 734-750
Miljkovic, N.D., Cooper, G.M., Marra, K.G., Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells (2008) Osteoarthritis Cartilage, 16 (10), pp. 1121-1130
Griffin, M., Iqbal, S.A., Bayat, A., Exploring the application of mesenchymal stem cells in bone repair and regeneration (2011) J Bone Joint Surg Br, 93 (4), pp. 427-434
Kim, I.S., Cho, T.H., Lee, Z.H., Hwang, S.J., Bone Regeneration by Transplantation of Human Mesenchymal Stromal Cells in a Rabbit Mandibular Distraction Osteogenesis Model (2012) Tissue Eng Part A, 19 (1-2), pp. 66-78
Bruder, S.P., Jaiswal, N., Haynesworth, S.E., Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation (1997) J Cell Biochem, 64 (2), pp. 278-294
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Bruder, S. P., Jaiswal, N., Haynesworth, S. E., Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation (1997) J Cell Biochem, 64 (2), pp. 278-294
Esposito, M. T., Di Noto, R., Mirabelli, P., Culture conditions allow selection of different mesenchymal progenitors from adult mouse bone marrow (2009) Tissue Eng Part A, 15 (9), pp. 2525-2536
Payumo, F. C., Kim, H. D., Sherling, M. A., Tissue engineering skeletal muscle for orthopaedic applications (2002) Clin Orthop Relat Res, (403 SUPPL.), pp. S228-S242
Liu, B., Wang, N. N., Wang, Z. L., Improved nude mouse models for green fluorescence human endometriosis (2010) J Obstet Gynaecol Res, 36 (6), pp. 1214-1221
Chang, P. C., Cirelli, J. A., Jin, Q., Adenovirus encoding human platelet-derived growth factor-B delivered to alveolar bone defects exhibits safety and biodistribution profiles favorable for clinical use (2009) Hum Gene Ther, 20 (5), pp. 486-496
Hu, W. W., Ward, B. B., Wang, Z., Krebsbach, P. H., Bone regeneration in defects compromised by radiotherapy (2010) J Dent Res, 89 (1), pp. 77-81
Thymidine Kinase-Mediated Shut Down of Bone Morphogenetic Protein-4 Expression Allows Regulated Bone Production
Bone morphogenetic Proteins (BMPs) are growth factors also involved in ossification and chondrogenesis that have generated interest for their efficiency in inducing bone neo-synthesis. BMPs expression in engineered cells has been successful in stimulating osteoblastic differentiation and ectopic and orthotopic bone formation in vivo. We have previously shown that an adenoviral vector expressing bone morphogenetic protein type-4 (BMP-4) is able to efficiently drive bone formation in a rabbit model of discontinuous bone lesions. However, unregulated secretion of BMPs has also been implicated in bone overproduction and exostosis. We have constructed a replication-defective first generation adenoviral (FG-Ad) vector containing a cassette for the expression of BMP-4 associated with the Herpes Simplex virus thymidine kinase (TK) gene (FG-B4TK) in order to shut down BMP-4 expression and, therefore, regulate bone production. TK expression does not interfere with BMP-4 ability to induce ectopic bone formation in athymic nude mice. Administration of ganciclovir blocks ectopic bone production in quadriceps muscle transduced with the FG-B4TK with no effect on the contralateral muscle transduced with a vector expressing only BMP-4. Histological findings confirmed the pro-apoptotic activity of TK and the reduction of mineralized areas in the quadriceps transduced with FG-B4TK in mice treated with ganciclovir. We have generated a system to block BMP-4 secretion by inducing apoptosis in transduced cells therefore blocking unwanted bone formation. This system is an additional tool to generate regulated amount of bone in discontinuous bone lesions and can be easily coupled with biomaterials capable of recruiting cells and generating a local bioreactor.
Bone morphogenetic Proteins (BMPs) are growth factors also involved in ossification and chondrogenesis that have generated interest for their efficiency in inducing bone neo-synthesis. BMPs expression in engineered cells has been successful in stimulating osteoblastic differentiation and ectopic and orthotopic bone formation in vivo. We have previously shown that an adenoviral vector expressing bone morphogenetic protein type-4 (BMP-4) is able to efficiently drive bone formation in a rabbit model of discontinuous bone lesions. However, unregulated secretion of BMPs has also been implicated in bone overproduction and exostosis. We have constructed a replication-defective first generation adenoviral (FG-Ad) vector containing a cassette for the expression of BMP-4 associated with the Herpes Simplex virus thymidine kinase (TK) gene (FG-B4TK) in order to shut down BMP-4 expression and, therefore, regulate bone production. TK expression does not interfere with BMP-4 ability to induce ectopic bone formation in athymic nude mice. Administration of ganciclovir blocks ectopic bone production in quadriceps muscle transduced with the FG-B4TK with no effect on the contralateral muscle transduced with a vector expressing only BMP-4. Histological findings confirmed the pro-apoptotic activity of TK and the reduction of mineralized areas in the quadriceps transduced with FG-B4TK in mice treated with ganciclovir. We have generated a system to block BMP-4 secretion by inducing apoptosis in transduced cells therefore blocking unwanted bone formation. This system is an additional tool to generate regulated amount of bone in discontinuous bone lesions and can be easily coupled with biomaterials capable of recruiting cells and generating a local bioreactor.
Thymidine Kinase-Mediated Shut Down of Bone Morphogenetic Protein-4 Expression Allows Regulated Bone Production
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Thymidine Kinase-Mediated Shut Down of Bone Morphogenetic Protein-4 Expression Allows Regulated Bone Production
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Impact Factor: 4.782
View Export to BibTeX Export to EndNote
Iaccarino G, Ciccarelli M, Sorriento D, Cipolletta E, Cerullo V, Iovino GL, Paudice A, Elia A, Santulli G, Campanile A, Arcucci O, Pastore L, Salvatore F, Condorelli G, Trimarco B
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Impact Factor: 12.563
View Export to BibTeX Export to EndNote
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* ONYX-015 Enhances Radiation-Induced Death of Human Anaplastic Thyroid Carcinoma Cells (468 views)
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Impact Factor: 5.873
View Export to BibTeX Export to EndNote
12 Records (10 excluding Abstracts).
Total impact factor: 64.108 (53.518 excluding Abstracts).
Total 5 year impact factor: 55.854 (45.671 excluding Abstracts).
Total impact factor: 64.108 (53.518 excluding Abstracts).
Total 5 year impact factor: 55.854 (45.671 excluding Abstracts).
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