Evaluation of gene expression and test of new therapies by preclinical imaging in murine models
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Evaluation of gene expression and test of new therapies by preclinical imaging in murine models
Research areas: Preclinical Imaging, 

Keywords: Gene expression, murine model, gene therapy, RNA interference therapy, preclinical imaging

Contact person: Sara Gargiulo, 

Actors: Sara Gargiulo,

Description:
In the last years murine models have become interesting tools to study gene expression and to test innovative classes of targeted therapeutics. RNA-mediated gene silencing and gene therapy hold particular interest in oncology, cardiovascular research, and in the bone regeneration field. Small-animal imaging has been an area of intense worldwide development in the last years, allowing to monitor changes at the cellular and molecular level in living animals, and refine preclinical research procedures, in agreement with the 3Rs principles. We demonstrated that morphological and functional imaging techniques are complementary tools to conventional in vitro methods for reflect the inhibition of tumor growth by RNA interference therapies, or quantify ectopic bone formation induced by virus-mediated gene delivering in living animals. We showed that in these circumstances, imaging allows to perform longitudinal studies, improving the accuracy of experimental results and reduce the number of animals involved into the research.


Figures:

High resolution computed tomography in mice: 3D volume rendering of mouse hindlimbs showing ectopic bone formation in the left femoral quadricep transduced with FG-B4TK adenoviral vector.

PET-CT fusion images with 3D volume rendering and simultaneous display of areas of FLT uptake in a medulloblastoma murine model: a wider bone defect in the occipito-parietal portion of the skull is visibile in the ADV5 MOCK medulloblastoma, with higher [18F]-FLT uptake; in AdV5-199b transfected tumor, the size was smaller and radiotracer uptake was lower.

Selected papers:

Papers

S. Gargiulo*, MP. Petretta*, A. Greco, MR. Panico, M. Larobina, M. Gramanzini, GG. Schiattarella, G. Esposito, M. Petretta, A. Brunetti, A. Cuocolo, “Genetic deletion in uncoupling protein 3 augments 18F-fluorodeoxyglucose cardiac uptake in the ischemic heart”, BMC Cardiovascular Disorders 2014 Aug 8; 14:98. * equal contribution

Forzati F, Federico A, Pallante P, Colamaio M, Esposito F, Sepe R, Gargiulo S, Luciano A, Arra C, Palma G, Bon G, Bucher S, Falcioni R, Brunetti A, Battista S, Fedele M, Fusco A. CBX7 gene expression plays a negative role in adipocyte cell growth and differentiation. Biol Open. 2014 Sep 4;3(9):871-9. doi: 10.1242/bio.20147872.

Lombardo B, Rocco T, Esposito MT, Cantilena B, Gargiulo S, Greco A, Montanaro D, Brunetti A, Pastore L.Thymidine Kinase-Mediated Shut Down of Bone Morphogenetic Protein-4 Expression Allows Regulated Bone Production. Curr Gene Ther. 2013 Jan 7.

Lignitto L, Arcella A, Sepe M, Rinaldi L, Delle Donne R, Gallo A, Stefan E, Bachmann VA, Oliva MA, Storlazzi CT, L’Abbate A, Brunetti A, Gargiulo S, Gramanzini M, Insabato L, Garbi C, Gottesman ME, Feliciello A. Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth. NATURE COMMUNICATIONS 2013 | 4:1822 | DOI: 10.1038/ncomms2791 | www.nature.com/naturecommunications

Garzia L, Andolfo I, Cusanelli E, Marino N, Petrosino G, De Martino D, Esposito V, Galeone A, Navas L, Esposito S, Gargiulo S, Fattet S, Donofrio V, Cinalli G, Brunetti A, Vecchio LD, Northcott PA, Delattre O, Taylor MD, Iolascon A, Zollo M.- MicroRNA-199b-5p impairs cancer stem cells through negative regulation of HES1 in medulloblastoma. PLoS One. 2009;4(3):e4998.


Abstract

Gargiulo S, Gramanzini M, Rocco T, Lombardo B , Brunetti A. High resolution micro-computed tomography evaluation of bone morphogenetic protein-4 expression in a mouse model. EMIM 2014, June 4-6, 2014, Stadsschouwburg Antwerp, Belgium.

M.Gramanzini, S.Gargiulo, A.Greco, A. Feliciello, L. Lignitto, A. Brunetti, M.Salvatore. [18]F-FLT PET and imaging post-processing for non-invasive assessment of siRNA treatment efficacy in experimental glioblastoma. EANM '13 - Annual Congress of the European Association of Nuclear Medicine, October 19-23, 2013 Lyon, France. (European Journal of Nuclear Medicine and Molecular Imaging Volume 40, Supplement 2 DOI 10.1007/s00259-013-2535-3)

M. Gramanzini, S. Gargiulo, L. Lignitto, A. Greco, A. Feliciello, A. Brunetti . [18F]FLT preclinical PET to evaluate the role of a novel cancer-associated gene in the aggressiveness of human glioma. AISAL 2012, Roma, Italia (Comparative Medicine Vol 62, No 6, December 2012, Pages 548-553)

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Evaluation of gene expression and test of new therapies by preclinical imaging in murine models
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Last modified by Sara Gargiulo on Wednesday 29 May 2019, 12:11:01
1197 views. Last view on Monday 17 March 2025, 17:57:53