Complementary use of preclinical imaging in minipig model studies
Contact person: Sara Gargiulo,
Actors: Sara Gargiulo,
Research areas: Preclinical Imaging,
Keywords: micro CT, minipig model, assessment for osseointegrated dental implants, 3D qualitative and quantitative image analyses
Contact person: Sara Gargiulo,
Actors: Sara Gargiulo,
Description:
Improvements
in bone-implant integration are crucial in modern dental surgery. The processes
of osseointegration involve an initial mechanical interlocking between alveolar
bone and the implant body (primary implant stability) and, later, a biological
fixation through continuous bone apposition (contact osteogenesis) and
remodeling toward the implant (secondary implant stability). The introduction
of newly developed implant materials for patients care often requires the use
of preclinical models to test biocompatibility, mechanical stability and
safety. Among other possible species, minipigs are a recommended animal model
for translational research of new dental materials before clinical trials in
humans, since their bone structure and physiology are similar to that of
humans. Based on the above considerations, minipig has been selected as a large
animal model for the pivotal preclinical testing of innovative biomaterials and
implants. This model would benefit from a non-destructive imaging modality so
that mechanical and morphological endpoints can more readily be examined in the
same specimens. Currently, microcomputed tomography (μCT) is a potential candidate tool as an adjunct for biomechanical
testing and for standard histology. μCT is fast, precise and, unlike conventional
microscopic methods, does not need long time for specimen preparation and is
not limited to assessment of a restricted number of sections. High-resolution μCT allows measurements of trabecular and cortical bone and a
three-dimensional (3D) representation of bone formation at the peri-implant
region. In this way, μCT allows less use of samples necessary for both
biomechanical and morphometric studies, in accordance with the principles of
the “refinement” and “reduction” declared by Russell and Burch in 1954. In our
pilot study we examined the utility of μCT for evaluating bone-implant integration in a
minipig model, as an adjunct to conventional histomorphometry and biomechanical
testing. The performance of an
innovative hybrid nanocomposite, as a biomimetic scaffold in improving implant
primary stability in post-surgical sites, were tested. μCT increases evaluation throughput and offers the possibility for
qualitative three-dimensional recording of the bone-implant system as well as
for non-destructive evaluation of bone architecture and mineral density, in
combination with conventional analysis methods. The proposed multimodal
approach allows to improve accuracy and reproducibility for peri-implant bone
measurements and could support future investigations.
Figures:
High resolution computed tomography in minipig: A, High-resolution μCT 3-D volume rendering
of minipig tibias with implants. B, Representative axial μCT image of
minipig tibias with implant-bone interface visualization. C, Representative
photograph of minipig tibias with implant position visualization. D-E,
High-resolution μCT 3-D volume rendering of scaffold. F, High-resolution μCT
3-D volume rendering peri-implant bone.
Selected papers:
Papers
Gramanzini M*, Gargiulo S*, Zarone F, Megna R, Apicella A,
Aversa R, Salvatore M, Mancini M, Sorrentino R, Brunetti A. Combined
microcomputed tomography, biomechanical and histomorphometric analysis of the
peri-implant bone: a pilot study in minipig model. Dental Materials 2016,
doi.org/10.1016/j.dental.2016.03.025 (In
press) * equal contribution
Abstract
Gramanzini M,
Gargiulo S, Sorrentino R, Apicella A, Zarone F, Brunetti A. Micro-CT Analysis
of Peri-implant bone in minipig model. EMIM 2014, June 4-6, 2014, Stadsschouwburg
Antwerp, Belgium.
Complementary use of preclinical imaging in minipig model studies
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Complementary use of preclinical imaging in minipig model studies
Search related bibliography on PubMed
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Gramanzini M, Gargiulo S, Zarone F, Megna R, Apicella A, Aversa R, Salvatore M, Mancini M, Sorrentino R, Brunetti A
* Combined microcomputed tomography, biomechanical and histomorphometric analysis of the peri-implant bone: a pilot study in minipig model (1909 views) (PDF 198 views)
Dent Mater (ISSN: 0109-5641, 0109-5641linking), 2016 Jun; 32(6): 794-806.
Impact Factor: 4.07
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* Combined microcomputed tomography, biomechanical and histomorphometric analysis of the peri-implant bone: a pilot study in minipig model (1909 views) (PDF 198 views)
Dent Mater (ISSN: 0109-5641, 0109-5641linking), 2016 Jun; 32(6): 794-806.
Impact Factor: 4.07
View Export to BibTeX Export to EndNote
1 Records (1 excluding Abstracts).
Total impact factor: 4.07 (4.07 excluding Abstracts).
Total 5 year impact factor: 5.155 (5.155 excluding Abstracts).
Total impact factor: 4.07 (4.07 excluding Abstracts).
Total 5 year impact factor: 5.155 (5.155 excluding Abstracts).
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