A synthetic strategy, based on expressed protein ligation, was developed to site-specific label TPR proteins with two different molecular probes

An innovative approach for the synthesis of dual modality peptide imaging probes based on the native chemical ligation approach. Hawala I, De Rosa L, Aime S, D'Andrea L D. Chemical Communications. 2020, 56, 3500-3503. DOI: 10.1039/C9CC09980H; Labeling of VEGFR1D2 through oxime ligation. De Rosa L, Di Stasi R, Longhitano L, D'Andrea L D. Bioorganic Chemmistry. 2019, 91:103160. DOI: 10.1016/j.bioorg.2019.103160; Total chemical synthesis by native chemical ligation of the all-D immunoglobulin-like domain 2 of Axl. De Rosa L, Di Stasi R, D’Andrea L D. Tetrahedron. 2019, 75, 894-905. DOI: 10.1016/j.tet.2019.01.005; Probing the Molecular Origin of Native-State Flexibility in Repeat Proteins. Cohen S S, Riven I, Cortajarena A L, De Rosa L, D'Andrea L D, Regan L, Haran G. Journal of the American Chemical Society. 2015, 137(32), 10367-73. DOI: 10.1021/jacs.5b06160; Semi-synthesis of labeled proteins for spectroscopic applications. De Rosa L, Russomanno A, Romanelli A, D'Andrea L D. Molecules. 2013;18(1):440-65. 10.3390/molecules18010440; Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins. De Rosa L, Cortajarena A L, Romanelli A, Regan L, D'Andrea L D. Organic & Biomolecular Chemistry. 2012, 10(2), 273-80. DOI: 10.1039/c1ob06397a; Semisynthesis of dimeric proteins by Expressed Protein Ligation. Ziaco B, Pensato S, D’Andrea L D, Benedetti E, Romanelli. A. Organic Letters. 2008, 10, 1955-19584. DOI: doi: 10.1021/ol800457g; Design of Stable α-Helical Arrays from an Idealized TPR Motif. Main ERG, Xiong Y, Cocco MJ, D’Andrea L D, Regan L. Structure. 2003, 11, 497-5082. DOI: 10.1016/S0969-2126(03)00076-5; TPR Proteins: The Versatile Helix. D’Andrea L D, Regan L. Trends in Biochemical. Sciences. 2003, 28, 655-6613. DOI: 10.1016/j.tibs.2003.10.007.